Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite
A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employ...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/2687341 |
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| author | Sabriye Yusan Mokhlesur M. Rahman Nasir Mohamad Tengku M. Arrif Ahmad Zubaidi A. Latif Mohd Aznan M. A. Wan Sani B. Wan Nik |
| author_facet | Sabriye Yusan Mokhlesur M. Rahman Nasir Mohamad Tengku M. Arrif Ahmad Zubaidi A. Latif Mohd Aznan M. A. Wan Sani B. Wan Nik |
| author_sort | Sabriye Yusan |
| collection | DOAJ |
| description | A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 × 10−5 to 2 × 10−3 M. Under optimal conditions, the biosensor exhibits high sensitivity (0.34 µA·mM−1), low detection limit (1 × 10−4 M), high affinity to glucose (Km = 0.02 mM), and also good reproducibility (R.S.D. 2.8%, n=10) and a stability of about ten days when stored dry at +4°C. Besides, the effects of pH value, scan rate, mediator effects on the glucose current, and electroactive interference of the biosensor were also discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility. |
| format | Article |
| id | doaj-art-05f1d8c3b2bb4316a3d501727c6d3313 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-05f1d8c3b2bb4316a3d501727c6d33132025-02-03T06:04:56ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/26873412687341Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous CompositeSabriye Yusan0Mokhlesur M. Rahman1Nasir Mohamad2Tengku M. Arrif3Ahmad Zubaidi A. Latif4Mohd Aznan M. A.5Wan Sani B. Wan Nik6Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, MalaysiaInstitute for Community Development & Quality of Life (i-CODE), Universiti Sultan Zainal Abidin, 21300 Kuala Nerus, Terengganu, MalaysiaInstitute for Community Development & Quality of Life (i-CODE), Universiti Sultan Zainal Abidin, 21300 Kuala Nerus, Terengganu, MalaysiaInstitute for Community Development & Quality of Life (i-CODE), Universiti Sultan Zainal Abidin, 21300 Kuala Nerus, Terengganu, MalaysiaFaculty of Medicine, Universiti Sultan Zainal Abidin, 21300 Kuala Nerus, Terengganu, MalaysiaFaculty of Medicine, International Islamic University Malaysia, 25200 Kuantan, MalaysiaSchool of Ocean Engineering, Universiti Malaysia Terengganu, 21300 Kuala Nerus, Terengganu, MalaysiaA new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 × 10−5 to 2 × 10−3 M. Under optimal conditions, the biosensor exhibits high sensitivity (0.34 µA·mM−1), low detection limit (1 × 10−4 M), high affinity to glucose (Km = 0.02 mM), and also good reproducibility (R.S.D. 2.8%, n=10) and a stability of about ten days when stored dry at +4°C. Besides, the effects of pH value, scan rate, mediator effects on the glucose current, and electroactive interference of the biosensor were also discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.http://dx.doi.org/10.1155/2018/2687341 |
| spellingShingle | Sabriye Yusan Mokhlesur M. Rahman Nasir Mohamad Tengku M. Arrif Ahmad Zubaidi A. Latif Mohd Aznan M. A. Wan Sani B. Wan Nik Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite Journal of Analytical Methods in Chemistry |
| title | Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite |
| title_full | Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite |
| title_fullStr | Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite |
| title_full_unstemmed | Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite |
| title_short | Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite |
| title_sort | development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the se mcm 41 mesoporous composite |
| url | http://dx.doi.org/10.1155/2018/2687341 |
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