Diagnostic Fragment-Ion-Based for Rapid Identification of Chlorogenic Acids Derivatives in Inula cappa Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry
Inula cappa (Buch.-Ham. ex D. Don) DC has been used in traditional Chinese medicine to treat malaria, dysentery, and hepatitis. Previous studies have shown that chlorogenic acid is the effective ingredient of plants in this family. And the research of the chlorogenic acid in Inula cappa will help to...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/6393246 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561333083570176 |
|---|---|
| author | Jie Peng Jing Xie Silin Shi Lilan Luo Kailin Li Pei Xiong Wei Cai |
| author_facet | Jie Peng Jing Xie Silin Shi Lilan Luo Kailin Li Pei Xiong Wei Cai |
| author_sort | Jie Peng |
| collection | DOAJ |
| description | Inula cappa (Buch.-Ham. ex D. Don) DC has been used in traditional Chinese medicine to treat malaria, dysentery, and hepatitis. Previous studies have shown that chlorogenic acid is the effective ingredient of plants in this family. And the research of the chlorogenic acid in Inula cappa will help to further improve the effective resource utilization rate of this plant. Therefore, it is necessary to establish an accurate method to characterize the chlorogenic acid components in Inula cappa. In this study, a simple, fast, and sensitive UHPLC-Q-Exactive Orbitrap mass spectrometry method was established, which can simultaneously analyze known and unknown ingredients in a short time (within 30 minutes) in Inula cappa. According to the diagnosis fragmentation ions, retention time, and bibliography, 68 chlorogenic acid derivatives were identified in Inula cappa. The results of this experiment lay the foundation for the active substances and quality control of Inula cappa and provide a theoretical basis for whether Inula cappa can be an alternative to the endangered wild medicinal materials of the same family. |
| format | Article |
| id | doaj-art-05cd207440b44e0389a8953d112d8df4 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-05cd207440b44e0389a8953d112d8df42025-02-03T01:25:13ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732021-01-01202110.1155/2021/63932466393246Diagnostic Fragment-Ion-Based for Rapid Identification of Chlorogenic Acids Derivatives in Inula cappa Using UHPLC-Q-Exactive Orbitrap Mass SpectrometryJie Peng0Jing Xie1Silin Shi2Lilan Luo3Kailin Li4Pei Xiong5Wei Cai6School of Pharmaceutical Sciences, Hunan Province Key Laboratory for Antibody-Based Drug and Intelligent Delivery System, Hunan University of Medicine, Huaihua 418000, ChinaDepartment of Rehabilitation Medicine and Health Care of Hunan Medical College, Hunan University of Medicine, Huaihua 418000, ChinaSchool of Pharmaceutical Sciences, Hunan Province Key Laboratory for Antibody-Based Drug and Intelligent Delivery System, Hunan University of Medicine, Huaihua 418000, ChinaSchool of Pharmaceutical Sciences, Hunan Province Key Laboratory for Antibody-Based Drug and Intelligent Delivery System, Hunan University of Medicine, Huaihua 418000, ChinaSchool of Pharmaceutical Sciences, Hunan Province Key Laboratory for Antibody-Based Drug and Intelligent Delivery System, Hunan University of Medicine, Huaihua 418000, ChinaSchool of Pharmaceutical Sciences, Hunan Province Key Laboratory for Antibody-Based Drug and Intelligent Delivery System, Hunan University of Medicine, Huaihua 418000, ChinaSchool of Pharmaceutical Sciences, Hunan Province Key Laboratory for Antibody-Based Drug and Intelligent Delivery System, Hunan University of Medicine, Huaihua 418000, ChinaInula cappa (Buch.-Ham. ex D. Don) DC has been used in traditional Chinese medicine to treat malaria, dysentery, and hepatitis. Previous studies have shown that chlorogenic acid is the effective ingredient of plants in this family. And the research of the chlorogenic acid in Inula cappa will help to further improve the effective resource utilization rate of this plant. Therefore, it is necessary to establish an accurate method to characterize the chlorogenic acid components in Inula cappa. In this study, a simple, fast, and sensitive UHPLC-Q-Exactive Orbitrap mass spectrometry method was established, which can simultaneously analyze known and unknown ingredients in a short time (within 30 minutes) in Inula cappa. According to the diagnosis fragmentation ions, retention time, and bibliography, 68 chlorogenic acid derivatives were identified in Inula cappa. The results of this experiment lay the foundation for the active substances and quality control of Inula cappa and provide a theoretical basis for whether Inula cappa can be an alternative to the endangered wild medicinal materials of the same family.http://dx.doi.org/10.1155/2021/6393246 |
| spellingShingle | Jie Peng Jing Xie Silin Shi Lilan Luo Kailin Li Pei Xiong Wei Cai Diagnostic Fragment-Ion-Based for Rapid Identification of Chlorogenic Acids Derivatives in Inula cappa Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry Journal of Analytical Methods in Chemistry |
| title | Diagnostic Fragment-Ion-Based for Rapid Identification of Chlorogenic Acids Derivatives in Inula cappa Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry |
| title_full | Diagnostic Fragment-Ion-Based for Rapid Identification of Chlorogenic Acids Derivatives in Inula cappa Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry |
| title_fullStr | Diagnostic Fragment-Ion-Based for Rapid Identification of Chlorogenic Acids Derivatives in Inula cappa Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry |
| title_full_unstemmed | Diagnostic Fragment-Ion-Based for Rapid Identification of Chlorogenic Acids Derivatives in Inula cappa Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry |
| title_short | Diagnostic Fragment-Ion-Based for Rapid Identification of Chlorogenic Acids Derivatives in Inula cappa Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry |
| title_sort | diagnostic fragment ion based for rapid identification of chlorogenic acids derivatives in inula cappa using uhplc q exactive orbitrap mass spectrometry |
| url | http://dx.doi.org/10.1155/2021/6393246 |
| work_keys_str_mv | AT jiepeng diagnosticfragmentionbasedforrapididentificationofchlorogenicacidsderivativesininulacappausinguhplcqexactiveorbitrapmassspectrometry AT jingxie diagnosticfragmentionbasedforrapididentificationofchlorogenicacidsderivativesininulacappausinguhplcqexactiveorbitrapmassspectrometry AT silinshi diagnosticfragmentionbasedforrapididentificationofchlorogenicacidsderivativesininulacappausinguhplcqexactiveorbitrapmassspectrometry AT lilanluo diagnosticfragmentionbasedforrapididentificationofchlorogenicacidsderivativesininulacappausinguhplcqexactiveorbitrapmassspectrometry AT kailinli diagnosticfragmentionbasedforrapididentificationofchlorogenicacidsderivativesininulacappausinguhplcqexactiveorbitrapmassspectrometry AT peixiong diagnosticfragmentionbasedforrapididentificationofchlorogenicacidsderivativesininulacappausinguhplcqexactiveorbitrapmassspectrometry AT weicai diagnosticfragmentionbasedforrapididentificationofchlorogenicacidsderivativesininulacappausinguhplcqexactiveorbitrapmassspectrometry |