Anti-Inflammatory Effect of IL-37b in Children with Allergic Rhinitis
Background. Interleukin-37 (IL-37), a newly described member of IL-1family, functioned as a fundamental inhibitor of innate inflammatory and immune responses, especially its isoform IL-37b. Objective. This study was undertaken to evaluate the expression and regulation of IL-37b in children with alle...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/746846 |
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| author | Wenlong Liu Li Deng Yanqiu Chen Changzhi Sun Jie Wang Lifeng Zhou Huabin Li Renzhong Luo |
| author_facet | Wenlong Liu Li Deng Yanqiu Chen Changzhi Sun Jie Wang Lifeng Zhou Huabin Li Renzhong Luo |
| author_sort | Wenlong Liu |
| collection | DOAJ |
| description | Background. Interleukin-37 (IL-37), a newly described member of IL-1family, functioned as a fundamental inhibitor of innate inflammatory and immune responses, especially its isoform IL-37b. Objective. This study was undertaken to evaluate the expression and regulation of IL-37b in children with allergic rhinitis (AR). Methods. Forty children with AR and twenty-five normal controls were included. The relationship between IL-37b and Th1/2 cytokines production in serum and nasal lavage was examined by enzyme-linked immunosorbent assay (ELISA). Peripheral blood mononuclear cells (PBMCs) were purified for in vitro regulation experiment of IL-37b. Intranasal mometasone furoate was given in AR children and IL-37b change after one-month treatment was detected using ELISA. Results. We observed significantly decreased IL-37b expression levels in both serum and nasal lavage compared to controls. IL-37b was negatively correlated with Th2 cytokines. Our results also showed that IL-37b downregulated Th2 cytokine expressed by PBMCs and this modulation was through mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathway. We also found that intranasal mometasone furoate therapy can promote nasal IL-37b expression. Conclusion. IL-37b may be involved in Th2 cytokine regulation in AR and its expression was related to the efficacy of intranasal steroid therapy. |
| format | Article |
| id | doaj-art-04a1e3bc25c54c02abbc040f650958b2 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-04a1e3bc25c54c02abbc040f650958b22025-02-03T05:53:30ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/746846746846Anti-Inflammatory Effect of IL-37b in Children with Allergic RhinitisWenlong Liu0Li Deng1Yanqiu Chen2Changzhi Sun3Jie Wang4Lifeng Zhou5Huabin Li6Renzhong Luo7Department of Otolaryngology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical College, No. 9 Jinsui Road, Guangzhou 510623, ChinaDepartment of Otolaryngology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical College, No. 9 Jinsui Road, Guangzhou 510623, ChinaDepartment of Otolaryngology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical College, No. 9 Jinsui Road, Guangzhou 510623, ChinaDepartment of Otolaryngology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical College, No. 9 Jinsui Road, Guangzhou 510623, ChinaDepartment of Otolaryngology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical College, No. 9 Jinsui Road, Guangzhou 510623, ChinaDepartment of Otolaryngology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical College, No. 9 Jinsui Road, Guangzhou 510623, ChinaDepartment of Otolaryngology, Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, No. 1665 Kongjiang Road, Shanghai 200092, ChinaDepartment of Otolaryngology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical College, No. 9 Jinsui Road, Guangzhou 510623, ChinaBackground. Interleukin-37 (IL-37), a newly described member of IL-1family, functioned as a fundamental inhibitor of innate inflammatory and immune responses, especially its isoform IL-37b. Objective. This study was undertaken to evaluate the expression and regulation of IL-37b in children with allergic rhinitis (AR). Methods. Forty children with AR and twenty-five normal controls were included. The relationship between IL-37b and Th1/2 cytokines production in serum and nasal lavage was examined by enzyme-linked immunosorbent assay (ELISA). Peripheral blood mononuclear cells (PBMCs) were purified for in vitro regulation experiment of IL-37b. Intranasal mometasone furoate was given in AR children and IL-37b change after one-month treatment was detected using ELISA. Results. We observed significantly decreased IL-37b expression levels in both serum and nasal lavage compared to controls. IL-37b was negatively correlated with Th2 cytokines. Our results also showed that IL-37b downregulated Th2 cytokine expressed by PBMCs and this modulation was through mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathway. We also found that intranasal mometasone furoate therapy can promote nasal IL-37b expression. Conclusion. IL-37b may be involved in Th2 cytokine regulation in AR and its expression was related to the efficacy of intranasal steroid therapy.http://dx.doi.org/10.1155/2014/746846 |
| spellingShingle | Wenlong Liu Li Deng Yanqiu Chen Changzhi Sun Jie Wang Lifeng Zhou Huabin Li Renzhong Luo Anti-Inflammatory Effect of IL-37b in Children with Allergic Rhinitis Mediators of Inflammation |
| title | Anti-Inflammatory Effect of IL-37b in Children with Allergic Rhinitis |
| title_full | Anti-Inflammatory Effect of IL-37b in Children with Allergic Rhinitis |
| title_fullStr | Anti-Inflammatory Effect of IL-37b in Children with Allergic Rhinitis |
| title_full_unstemmed | Anti-Inflammatory Effect of IL-37b in Children with Allergic Rhinitis |
| title_short | Anti-Inflammatory Effect of IL-37b in Children with Allergic Rhinitis |
| title_sort | anti inflammatory effect of il 37b in children with allergic rhinitis |
| url | http://dx.doi.org/10.1155/2014/746846 |
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