IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease
Although interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet. The objective of this study was to investigate the association of IL17A and IL17F genes po...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/571231 |
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| author | Renata Gonçalves Resende Jeane de Fátima Correia-Silva Tarcília Aparecida Silva Ulisses Eliezer Salomão Luciano Marques-Silva Érica Leandro Marciano Vieira Walderez Ornelas Dutra Ricardo Santiago Gomez |
| author_facet | Renata Gonçalves Resende Jeane de Fátima Correia-Silva Tarcília Aparecida Silva Ulisses Eliezer Salomão Luciano Marques-Silva Érica Leandro Marciano Vieira Walderez Ornelas Dutra Ricardo Santiago Gomez |
| author_sort | Renata Gonçalves Resende |
| collection | DOAJ |
| description | Although interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet. The objective of this study was to investigate the association of IL17A and IL17F genes polymorphisms and IL-17A and IL-17F levels with cGVHD. IL-17A expression was also investigated in CD4+ T cells of patients with systemic cGVHD. For Part I of the study, fifty-eight allo-HSCT recipients and donors were prospectively studied. Blood samples were obtained to determine IL17A and IL17F genes polymorphisms. Cytokines levels in blood and saliva were assessed by ELISA at days +35 and +100 after HSCT. In Part II, for the immunophenotypic evaluation, eight patients with systemic cGVHD were selected and the expression of IL-17A was evaluated. We found association between recipient AA genotype with systemic cGVHD. No association was observed between IL-17A levels and cGVHD. Lower IL-17A levels in the blood were associated with AA genotype. In flow cytometry analysis, decreased expression of IL-17A was observed in patients with cGVHD after stimulation. In conclusion, IL-17A may have an important role in the development of systemic cGVHD. |
| format | Article |
| id | doaj-art-04820285052b4bb99a5bbdfc4e91255f |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-04820285052b4bb99a5bbdfc4e91255f2025-02-03T01:10:54ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/571231571231IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host DiseaseRenata Gonçalves Resende0Jeane de Fátima Correia-Silva1Tarcília Aparecida Silva2Ulisses Eliezer Salomão3Luciano Marques-Silva4Érica Leandro Marciano Vieira5Walderez Ornelas Dutra6Ricardo Santiago Gomez7Serviço de Estomatologia, Hospital Municipal Odilon Behrens, Rua Formiga 50, 31110-430 Belo Horizonte, MG, BrazilCentro Universitário Newton Paiva, Faculdade de Odontologia, Avenida Presidente Carlos Luz 220, 31230-010 Belo Horizonte, MG, BrazilDepartamento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Odontologia Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilCiodonto-Universidade FACSETE, Rua Itália Pontelo 50, 35700-170 Sete Lagoas, MG, BrazilLaboratório Interdisciplinar de Pesquisa Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Avenida Alfredo Balena 190, 30130-10 Belo Horizonte, MG, BrazilDepartamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilAlthough interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet. The objective of this study was to investigate the association of IL17A and IL17F genes polymorphisms and IL-17A and IL-17F levels with cGVHD. IL-17A expression was also investigated in CD4+ T cells of patients with systemic cGVHD. For Part I of the study, fifty-eight allo-HSCT recipients and donors were prospectively studied. Blood samples were obtained to determine IL17A and IL17F genes polymorphisms. Cytokines levels in blood and saliva were assessed by ELISA at days +35 and +100 after HSCT. In Part II, for the immunophenotypic evaluation, eight patients with systemic cGVHD were selected and the expression of IL-17A was evaluated. We found association between recipient AA genotype with systemic cGVHD. No association was observed between IL-17A levels and cGVHD. Lower IL-17A levels in the blood were associated with AA genotype. In flow cytometry analysis, decreased expression of IL-17A was observed in patients with cGVHD after stimulation. In conclusion, IL-17A may have an important role in the development of systemic cGVHD.http://dx.doi.org/10.1155/2014/571231 |
| spellingShingle | Renata Gonçalves Resende Jeane de Fátima Correia-Silva Tarcília Aparecida Silva Ulisses Eliezer Salomão Luciano Marques-Silva Érica Leandro Marciano Vieira Walderez Ornelas Dutra Ricardo Santiago Gomez IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease Mediators of Inflammation |
| title | IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease |
| title_full | IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease |
| title_fullStr | IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease |
| title_full_unstemmed | IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease |
| title_short | IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease |
| title_sort | il 17 genetic and immunophenotypic evaluation in chronic graft versus host disease |
| url | http://dx.doi.org/10.1155/2014/571231 |
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