Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits
Growth is a priority trait from the point of view of genetic improvement. Molecular markers linked to quantitative trait loci (QTL) have been regarded as useful for marker-assisted selection in complex traits as growth. Polymorphisms have been studied in five candidate genes influencing growth in gi...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/369802 |
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| author | Irma Sánchez-Ramos Ismael Cross Jaroslav Mácha Gonzalo Martínez-Rodríguez Vladimir Krylov Laureana Rebordinos |
| author_facet | Irma Sánchez-Ramos Ismael Cross Jaroslav Mácha Gonzalo Martínez-Rodríguez Vladimir Krylov Laureana Rebordinos |
| author_sort | Irma Sánchez-Ramos |
| collection | DOAJ |
| description | Growth is a priority trait from the point of view of genetic improvement. Molecular markers linked to quantitative trait loci (QTL) have been regarded as useful for marker-assisted selection in complex traits as growth. Polymorphisms have been studied in five candidate genes influencing growth in gilthead seabream (Sparus aurata): the growth hormone (GH), insulin-like growth factor-1 (IGF-1), myostatin (MSTN-1), prolactin (PRL), and somatolactin (SL) genes. Specimens evaluated were from a commercial broodstock comprising 131 breeders (from which 36 males and 44 females contributed to the progeny). In all samples eleven gene fragments, covering more than 13,000 bp, generated by PCR-RFLP, were analyzed; tests were made for significant associations between these markers and growth traits. ANOVA results showed a significant association between MSTN-1 gene polymorphism and growth traits. Pairwise tests revealed several RFLPs in the MSTN-1 gene with significant heterogeneity of genotypes among size groups. PRL and MSTN-1 genes presented linkage disequilibrium. The MSTN-1 gene was mapped in the centromeric region of a medium-size acrocentric chromosome pair. |
| format | Article |
| id | doaj-art-045a3090ceea439caa8b0089b62590d7 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-045a3090ceea439caa8b0089b62590d72025-02-03T01:11:48ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/369802369802Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth TraitsIrma Sánchez-Ramos0Ismael Cross1Jaroslav Mácha2Gonzalo Martínez-Rodríguez3Vladimir Krylov4Laureana Rebordinos5Laboratorio de Genética, Universidad de Cádiz, Poligono Río San Pedro s/n, 11510 Puerto Real, SpainLaboratorio de Genética, Universidad de Cádiz, Poligono Río San Pedro s/n, 11510 Puerto Real, SpainDepartment of Cell Biology, Faculty of Science, Charles University in Prague, Prague 2, Viničná 7, 12843 Prague, Czech RepublicInstituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas, República Saharaui, no. 2, 11510 Puerto Real, SpainDepartment of Cell Biology, Faculty of Science, Charles University in Prague, Prague 2, Viničná 7, 12843 Prague, Czech RepublicLaboratorio de Genética, Universidad de Cádiz, Poligono Río San Pedro s/n, 11510 Puerto Real, SpainGrowth is a priority trait from the point of view of genetic improvement. Molecular markers linked to quantitative trait loci (QTL) have been regarded as useful for marker-assisted selection in complex traits as growth. Polymorphisms have been studied in five candidate genes influencing growth in gilthead seabream (Sparus aurata): the growth hormone (GH), insulin-like growth factor-1 (IGF-1), myostatin (MSTN-1), prolactin (PRL), and somatolactin (SL) genes. Specimens evaluated were from a commercial broodstock comprising 131 breeders (from which 36 males and 44 females contributed to the progeny). In all samples eleven gene fragments, covering more than 13,000 bp, generated by PCR-RFLP, were analyzed; tests were made for significant associations between these markers and growth traits. ANOVA results showed a significant association between MSTN-1 gene polymorphism and growth traits. Pairwise tests revealed several RFLPs in the MSTN-1 gene with significant heterogeneity of genotypes among size groups. PRL and MSTN-1 genes presented linkage disequilibrium. The MSTN-1 gene was mapped in the centromeric region of a medium-size acrocentric chromosome pair.http://dx.doi.org/10.1100/2012/369802 |
| spellingShingle | Irma Sánchez-Ramos Ismael Cross Jaroslav Mácha Gonzalo Martínez-Rodríguez Vladimir Krylov Laureana Rebordinos Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits The Scientific World Journal |
| title | Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits |
| title_full | Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits |
| title_fullStr | Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits |
| title_full_unstemmed | Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits |
| title_short | Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits |
| title_sort | assessment of tools for marker assisted selection in a marine commercial species significant association between mstn 1 gene polymorphism and growth traits |
| url | http://dx.doi.org/10.1100/2012/369802 |
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