Responses of Phospholipase D and Antioxidant System to Mechanical Wounding in Postharvest Banana Fruits
Banana fruits are susceptible to mechanical damage. The present study was to investigate the responses of phospholipase D (PLD) and antioxidant system to mechanical wounding in postharvest banana fruits. During 16 d storage at 25°C and 90% relative humidity, PLD activity in wounded fruits was signif...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2017/8347306 |
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| author | Li Li Xuemei He Jian Sun Changbao Li Dongning Ling Jinfeng Sheng Fengjin Zheng Guoming Liu Jiemin Li Yayuan Tang Ping Yi Ming Xin Zhichun Li Zhugui Zhou |
| author_facet | Li Li Xuemei He Jian Sun Changbao Li Dongning Ling Jinfeng Sheng Fengjin Zheng Guoming Liu Jiemin Li Yayuan Tang Ping Yi Ming Xin Zhichun Li Zhugui Zhou |
| author_sort | Li Li |
| collection | DOAJ |
| description | Banana fruits are susceptible to mechanical damage. The present study was to investigate the responses of phospholipase D (PLD) and antioxidant system to mechanical wounding in postharvest banana fruits. During 16 d storage at 25°C and 90% relative humidity, PLD activity in wounded fruits was significantly higher than that in control (without artificial wounding fruits). The higher value of PLD mRNA was found in wounded fruits than in control. PLD mRNA expression reached the highest peak on day 4 in both groups, but it was 2.67 times in wounded fruits compared to control at that time, indicating that PLD gene expression was activated in response to wounding stress. In response to wounding stress, the higher lipoxygenase (LOX) activity was observed and malondialdehyde (MDA) production was accelerated. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) in wounded fruits were significantly higher than those in control. The concentrations of reactive oxygen species (ROS) such as superoxide anion (O2•-) and hydrogen peroxide (H2O2) in fruits increased under mechanical wounding. The above results provided a basis for further investigating the mechanism of postharvest banana fruits adapting to environmental stress. |
| format | Article |
| id | doaj-art-0444bbf7a8ac4cebb46a10b109405800 |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-0444bbf7a8ac4cebb46a10b1094058002025-02-03T01:28:56ZengWileyJournal of Food Quality0146-94281745-45572017-01-01201710.1155/2017/83473068347306Responses of Phospholipase D and Antioxidant System to Mechanical Wounding in Postharvest Banana FruitsLi Li0Xuemei He1Jian Sun2Changbao Li3Dongning Ling4Jinfeng Sheng5Fengjin Zheng6Guoming Liu7Jiemin Li8Yayuan Tang9Ping Yi10Ming Xin11Zhichun Li12Zhugui Zhou13Agro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaAgro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning 530007, ChinaBanana fruits are susceptible to mechanical damage. The present study was to investigate the responses of phospholipase D (PLD) and antioxidant system to mechanical wounding in postharvest banana fruits. During 16 d storage at 25°C and 90% relative humidity, PLD activity in wounded fruits was significantly higher than that in control (without artificial wounding fruits). The higher value of PLD mRNA was found in wounded fruits than in control. PLD mRNA expression reached the highest peak on day 4 in both groups, but it was 2.67 times in wounded fruits compared to control at that time, indicating that PLD gene expression was activated in response to wounding stress. In response to wounding stress, the higher lipoxygenase (LOX) activity was observed and malondialdehyde (MDA) production was accelerated. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) in wounded fruits were significantly higher than those in control. The concentrations of reactive oxygen species (ROS) such as superoxide anion (O2•-) and hydrogen peroxide (H2O2) in fruits increased under mechanical wounding. The above results provided a basis for further investigating the mechanism of postharvest banana fruits adapting to environmental stress.http://dx.doi.org/10.1155/2017/8347306 |
| spellingShingle | Li Li Xuemei He Jian Sun Changbao Li Dongning Ling Jinfeng Sheng Fengjin Zheng Guoming Liu Jiemin Li Yayuan Tang Ping Yi Ming Xin Zhichun Li Zhugui Zhou Responses of Phospholipase D and Antioxidant System to Mechanical Wounding in Postharvest Banana Fruits Journal of Food Quality |
| title | Responses of Phospholipase D and Antioxidant System to Mechanical Wounding in Postharvest Banana Fruits |
| title_full | Responses of Phospholipase D and Antioxidant System to Mechanical Wounding in Postharvest Banana Fruits |
| title_fullStr | Responses of Phospholipase D and Antioxidant System to Mechanical Wounding in Postharvest Banana Fruits |
| title_full_unstemmed | Responses of Phospholipase D and Antioxidant System to Mechanical Wounding in Postharvest Banana Fruits |
| title_short | Responses of Phospholipase D and Antioxidant System to Mechanical Wounding in Postharvest Banana Fruits |
| title_sort | responses of phospholipase d and antioxidant system to mechanical wounding in postharvest banana fruits |
| url | http://dx.doi.org/10.1155/2017/8347306 |
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