Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig
Olfactory receptors (ORs) are members of the transmembrane G protein-coupled receptor superfamily, playing a crucial role in odor recognition, which further mediates crucial biological processes in mammals. In sows, androstenone can trigger sexual behaviors through olfaction, but the underlying mech...
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2024-12-01
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| author | Peidong Yang Tingting Luo Shuqi Yang Anjing Zhang Yuan Tang Li Chen Jinyong Wang Yongju Zhao Zhining Zhong Xuemin Li Ziyin Han Yupei Zhang Yue Tang Jideng Ma Long Jin Keren Long Mingzhou Li Lu Lu |
| author_facet | Peidong Yang Tingting Luo Shuqi Yang Anjing Zhang Yuan Tang Li Chen Jinyong Wang Yongju Zhao Zhining Zhong Xuemin Li Ziyin Han Yupei Zhang Yue Tang Jideng Ma Long Jin Keren Long Mingzhou Li Lu Lu |
| author_sort | Peidong Yang |
| collection | DOAJ |
| description | Olfactory receptors (ORs) are members of the transmembrane G protein-coupled receptor superfamily, playing a crucial role in odor recognition, which further mediates crucial biological processes in mammals. In sows, androstenone can trigger sexual behaviors through olfaction, but the underlying mechanism remains to be explored. To efficiently and accurately screen pig olfactory receptors responding to androstenone and the key structure determinant, we adapted the high-throughput RNA-seq strategy to screen the altered genes upon androstenone treatment in the olfactory epithelium of pigs, yielding 1397 downregulated genes. Of which, 15 OR genes and 49 OR-like genes were candidate androstenone-responsive genes, and 5 ORs (OR2D2, OR8D1, OR8D2, OR10Z1 and OR7D4) were proven as responsible for androstenone-mediated olfaction in vitro. Among the five ORs, pig OR7D4 has the highest level of androstenone response. To further find the structural determinant, we performed ligand-binding cavity analysis on pig OR7D4 with androstenone, predicted seven potential structural sites and further confirmed that F178 and T203 are the key sites for recognizing androstenone. Nevertheless, the natural non-synonymous mutation M133V (rs696400829) of pig OR7D4 was proven to significantly impair the respondence to androstenone. This is the first time the ORs responding to androstenone in pigs and the key structural determinant of pig OR7D4 were identified, which highlights the significance of investigating the role of OR7D4 in pig reproduction performance in the future. |
| format | Article |
| id | doaj-art-03de1c7f495b431e831eb82eb674056b |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-03de1c7f495b431e831eb82eb674056b2025-01-24T13:27:23ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-12-014711310.3390/cimb47010013Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic PigPeidong Yang0Tingting Luo1Shuqi Yang2Anjing Zhang3Yuan Tang4Li Chen5Jinyong Wang6Yongju Zhao7Zhining Zhong8Xuemin Li9Ziyin Han10Yupei Zhang11Yue Tang12Jideng Ma13Long Jin14Keren Long15Mingzhou Li16Lu Lu17State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaDepartment of Pig Production, Chongqing Academy of Animal Science, Chongqing 402460, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaDepartment of Pig Production, Chongqing Academy of Animal Science, Chongqing 402460, ChinaDepartment of Pig Production, Chongqing Academy of Animal Science, Chongqing 402460, ChinaCollege of Animal Science and Technology, Southwest University, Chongqing 402460, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 610000, ChinaOlfactory receptors (ORs) are members of the transmembrane G protein-coupled receptor superfamily, playing a crucial role in odor recognition, which further mediates crucial biological processes in mammals. In sows, androstenone can trigger sexual behaviors through olfaction, but the underlying mechanism remains to be explored. To efficiently and accurately screen pig olfactory receptors responding to androstenone and the key structure determinant, we adapted the high-throughput RNA-seq strategy to screen the altered genes upon androstenone treatment in the olfactory epithelium of pigs, yielding 1397 downregulated genes. Of which, 15 OR genes and 49 OR-like genes were candidate androstenone-responsive genes, and 5 ORs (OR2D2, OR8D1, OR8D2, OR10Z1 and OR7D4) were proven as responsible for androstenone-mediated olfaction in vitro. Among the five ORs, pig OR7D4 has the highest level of androstenone response. To further find the structural determinant, we performed ligand-binding cavity analysis on pig OR7D4 with androstenone, predicted seven potential structural sites and further confirmed that F178 and T203 are the key sites for recognizing androstenone. Nevertheless, the natural non-synonymous mutation M133V (rs696400829) of pig OR7D4 was proven to significantly impair the respondence to androstenone. This is the first time the ORs responding to androstenone in pigs and the key structural determinant of pig OR7D4 were identified, which highlights the significance of investigating the role of OR7D4 in pig reproduction performance in the future.https://www.mdpi.com/1467-3045/47/1/13pigolfactory receptorsandrostenonetranscriptomeOR7D4 |
| spellingShingle | Peidong Yang Tingting Luo Shuqi Yang Anjing Zhang Yuan Tang Li Chen Jinyong Wang Yongju Zhao Zhining Zhong Xuemin Li Ziyin Han Yupei Zhang Yue Tang Jideng Ma Long Jin Keren Long Mingzhou Li Lu Lu Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig Current Issues in Molecular Biology pig olfactory receptors androstenone transcriptome OR7D4 |
| title | Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig |
| title_full | Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig |
| title_fullStr | Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig |
| title_full_unstemmed | Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig |
| title_short | Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig |
| title_sort | identification of olfactory receptors responding to androstenone and the key structure determinant in domestic pig |
| topic | pig olfactory receptors androstenone transcriptome OR7D4 |
| url | https://www.mdpi.com/1467-3045/47/1/13 |
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