First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection Status
Small ruminant lentiviruses (SRLVs) infect sheep, causing a multiorganic disease called maedi-visna or ovine progressive pneumonia, which significantly affects the production and welfare of sheep, generating serious economic losses. Although not all infected animals develop fully symptomatic disease...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/14/1/16 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587756661899264 |
|---|---|
| author | Magdalena Materniak-Kornas Katarzyna Piórkowska Katarzyna Ropka-Molik Adrianna Dominika Musiał Joanna Kowalik Anna Kycko Jacek Kuźmak |
| author_facet | Magdalena Materniak-Kornas Katarzyna Piórkowska Katarzyna Ropka-Molik Adrianna Dominika Musiał Joanna Kowalik Anna Kycko Jacek Kuźmak |
| author_sort | Magdalena Materniak-Kornas |
| collection | DOAJ |
| description | Small ruminant lentiviruses (SRLVs) infect sheep, causing a multiorganic disease called maedi-visna or ovine progressive pneumonia, which significantly affects the production and welfare of sheep, generating serious economic losses. Although not all infected animals develop fully symptomatic disease, they constantly spread the virus in the flock. Since the infection is incurable and no vaccine is available, another approach is necessary to control SRLV infections. In recent years, an alternative for culling infected animals has become the approach based on identifying genetic markers for selecting SRLV-resistant individuals. Recent reports revealed several candidates, including gene encoding transmembrane protein 154 (TMEM154). Several single nucleotide polymorphisms (SNPs) are found within this gene in sheep of different breeds, but only some can be considered as resistant markers. This study aimed to investigate the presence of single polymorphic sites in TMEM154 gene in sheep of selected Polish flocks and assess their association with the infection and proviral load in the context of susceptibility to SRLV infection. In total 107 sheep, representing three breeds, were screened for their SRLV infection status by serological and PCR testing. All these animals were also genotyped to characterize the presence of SNPs in TMEM154 gene and estimate their potential of being the SRLV-resistance marker. The frequency of identified alleles differed among breeds. Moreover, the positive association between TMEM154 genotype and SRLV status was found for E35K polymorphism and two polymorphic sites in 5′UTR in one of analyzed breed. However, when the relationship between SNPs and SRLV proviral load was analyzed, five had a strong association, considering the whole population of tested sheep. Presented data, for the first time, identified the presence of SNPs in TMEM154 gene in sheep housed in Polish flocks and suggested that selecting SRLV-resistant animals based on this analysis might be possible, but further validation in a larger group of sheep is required. |
| format | Article |
| id | doaj-art-03c4e7c854d94be49806d5378c974bf4 |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-03c4e7c854d94be49806d5378c974bf42025-01-24T13:44:36ZengMDPI AGPathogens2076-08172024-12-011411610.3390/pathogens14010016First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection StatusMagdalena Materniak-Kornas0Katarzyna Piórkowska1Katarzyna Ropka-Molik2Adrianna Dominika Musiał3Joanna Kowalik4Anna Kycko5Jacek Kuźmak6Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, PolandDepartment of Animal Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, PolandDepartment of Animal Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, PolandDepartment of Animal Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, PolandDepartment of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, PolandDepartment of Pathology, National Veterinary Research Institute, 24-100 Pulawy, PolandDepartment of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, PolandSmall ruminant lentiviruses (SRLVs) infect sheep, causing a multiorganic disease called maedi-visna or ovine progressive pneumonia, which significantly affects the production and welfare of sheep, generating serious economic losses. Although not all infected animals develop fully symptomatic disease, they constantly spread the virus in the flock. Since the infection is incurable and no vaccine is available, another approach is necessary to control SRLV infections. In recent years, an alternative for culling infected animals has become the approach based on identifying genetic markers for selecting SRLV-resistant individuals. Recent reports revealed several candidates, including gene encoding transmembrane protein 154 (TMEM154). Several single nucleotide polymorphisms (SNPs) are found within this gene in sheep of different breeds, but only some can be considered as resistant markers. This study aimed to investigate the presence of single polymorphic sites in TMEM154 gene in sheep of selected Polish flocks and assess their association with the infection and proviral load in the context of susceptibility to SRLV infection. In total 107 sheep, representing three breeds, were screened for their SRLV infection status by serological and PCR testing. All these animals were also genotyped to characterize the presence of SNPs in TMEM154 gene and estimate their potential of being the SRLV-resistance marker. The frequency of identified alleles differed among breeds. Moreover, the positive association between TMEM154 genotype and SRLV status was found for E35K polymorphism and two polymorphic sites in 5′UTR in one of analyzed breed. However, when the relationship between SNPs and SRLV proviral load was analyzed, five had a strong association, considering the whole population of tested sheep. Presented data, for the first time, identified the presence of SNPs in TMEM154 gene in sheep housed in Polish flocks and suggested that selecting SRLV-resistant animals based on this analysis might be possible, but further validation in a larger group of sheep is required.https://www.mdpi.com/2076-0817/14/1/16small ruminant lentiviruses (SRLVs)sheepgenotypingTMEM154single nucleotide polymorphism |
| spellingShingle | Magdalena Materniak-Kornas Katarzyna Piórkowska Katarzyna Ropka-Molik Adrianna Dominika Musiał Joanna Kowalik Anna Kycko Jacek Kuźmak First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection Status Pathogens small ruminant lentiviruses (SRLVs) sheep genotyping TMEM154 single nucleotide polymorphism |
| title | First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection Status |
| title_full | First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection Status |
| title_fullStr | First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection Status |
| title_full_unstemmed | First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection Status |
| title_short | First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection Status |
| title_sort | first report of snps detection in tmem154 gene in sheep from poland and their association with srlv infection status |
| topic | small ruminant lentiviruses (SRLVs) sheep genotyping TMEM154 single nucleotide polymorphism |
| url | https://www.mdpi.com/2076-0817/14/1/16 |
| work_keys_str_mv | AT magdalenamaterniakkornas firstreportofsnpsdetectionintmem154geneinsheepfrompolandandtheirassociationwithsrlvinfectionstatus AT katarzynapiorkowska firstreportofsnpsdetectionintmem154geneinsheepfrompolandandtheirassociationwithsrlvinfectionstatus AT katarzynaropkamolik firstreportofsnpsdetectionintmem154geneinsheepfrompolandandtheirassociationwithsrlvinfectionstatus AT adriannadominikamusiał firstreportofsnpsdetectionintmem154geneinsheepfrompolandandtheirassociationwithsrlvinfectionstatus AT joannakowalik firstreportofsnpsdetectionintmem154geneinsheepfrompolandandtheirassociationwithsrlvinfectionstatus AT annakycko firstreportofsnpsdetectionintmem154geneinsheepfrompolandandtheirassociationwithsrlvinfectionstatus AT jacekkuzmak firstreportofsnpsdetectionintmem154geneinsheepfrompolandandtheirassociationwithsrlvinfectionstatus |