Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes
Articular chondrocytes are responsible for the maintenance of healthy articulations; indeed, dysregulation of their functions, including the production of matrix proteins and matrix-remodeling proteases, may result in fraying of the tissue and development of osteoarthritis (OA). To explore transcrip...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/318793 |
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| author | Maria Mesuraca Olimpio Galasso Leonardo Guido Emanuela Chiarella Stefania Scicchitano Renaud Vatrinet Giovanni Morrone Heather M. Bond Giorgio Gasparini |
| author_facet | Maria Mesuraca Olimpio Galasso Leonardo Guido Emanuela Chiarella Stefania Scicchitano Renaud Vatrinet Giovanni Morrone Heather M. Bond Giorgio Gasparini |
| author_sort | Maria Mesuraca |
| collection | DOAJ |
| description | Articular chondrocytes are responsible for the maintenance of healthy articulations; indeed, dysregulation of their functions, including the production of matrix proteins and matrix-remodeling proteases, may result in fraying of the tissue and development of osteoarthritis (OA). To explore transcriptional mechanisms that contribute to the regulation of chondrocyte homeostasis and may be implicated in OA development, we compared the gene expression profile of a set of zinc finger proteins potentially linked to the control of chondrocyte differentiation and/or functions (ZNF423, ZNF470, ZNF521, and ZNF780B) in chondrocytes from patients affected by OA and from subjects not affected by OA. This analysis highlighted a significantly lower expression of the transcript encoding ZNF423 in chondrocytes from OA, particularly in elderly patients. Interestingly, this decrease was mirrored by the similarly reduced expression of PPARγ, a known target of ZNF423 with anti-inflammatory and chondroprotective properties. The ZNF521 mRNA instead was abundant in all primary chondrocytes studied; the RNAi-mediated silencing of this gene significantly altered the COL2A/COL1 expression ratio, associated with the maintenance of the differentiated phenotype, in chondrocytes cultivated in alginate beads. These results suggest a role for ZNF423 and ZNF521 in the regulation of chondrocyte homeostasis and warrant further investigations to elucidate their mechanism of action. |
| format | Article |
| id | doaj-art-02b904ec994b40b4b0d51b73c796eeb7 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-02b904ec994b40b4b0d51b73c796eeb72025-02-03T01:27:31ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/318793318793Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular ChondrocytesMaria Mesuraca0Olimpio Galasso1Leonardo Guido2Emanuela Chiarella3Stefania Scicchitano4Renaud Vatrinet5Giovanni Morrone6Heather M. Bond7Giorgio Gasparini8Laboratory of Molecular Haematopoiesis and Stem Cell Biology, Department of Experimental and Clinical Medicine, University of Catanzaro Magna Græcia, University Campus “Salvatore Venuta”, Germaneto, 88100 Catanzaro, ItalyOrthopedic and Trauma Surgery, Department of Medical and Surgical Sciences, University of Catanzaro Magna Græcia, University Campus “Salvatore Venuta”, Germaneto, 88100 Catanzaro, ItalyOrthopedic and Trauma Surgery, Department of Medical and Surgical Sciences, University of Catanzaro Magna Græcia, University Campus “Salvatore Venuta”, Germaneto, 88100 Catanzaro, ItalyLaboratory of Molecular Haematopoiesis and Stem Cell Biology, Department of Experimental and Clinical Medicine, University of Catanzaro Magna Græcia, University Campus “Salvatore Venuta”, Germaneto, 88100 Catanzaro, ItalyLaboratory of Molecular Haematopoiesis and Stem Cell Biology, Department of Experimental and Clinical Medicine, University of Catanzaro Magna Græcia, University Campus “Salvatore Venuta”, Germaneto, 88100 Catanzaro, ItalyOrthopedic and Trauma Surgery, Department of Medical and Surgical Sciences, University of Catanzaro Magna Græcia, University Campus “Salvatore Venuta”, Germaneto, 88100 Catanzaro, ItalyLaboratory of Molecular Haematopoiesis and Stem Cell Biology, Department of Experimental and Clinical Medicine, University of Catanzaro Magna Græcia, University Campus “Salvatore Venuta”, Germaneto, 88100 Catanzaro, ItalyLaboratory of Molecular Haematopoiesis and Stem Cell Biology, Department of Experimental and Clinical Medicine, University of Catanzaro Magna Græcia, University Campus “Salvatore Venuta”, Germaneto, 88100 Catanzaro, ItalyOrthopedic and Trauma Surgery, Department of Medical and Surgical Sciences, University of Catanzaro Magna Græcia, University Campus “Salvatore Venuta”, Germaneto, 88100 Catanzaro, ItalyArticular chondrocytes are responsible for the maintenance of healthy articulations; indeed, dysregulation of their functions, including the production of matrix proteins and matrix-remodeling proteases, may result in fraying of the tissue and development of osteoarthritis (OA). To explore transcriptional mechanisms that contribute to the regulation of chondrocyte homeostasis and may be implicated in OA development, we compared the gene expression profile of a set of zinc finger proteins potentially linked to the control of chondrocyte differentiation and/or functions (ZNF423, ZNF470, ZNF521, and ZNF780B) in chondrocytes from patients affected by OA and from subjects not affected by OA. This analysis highlighted a significantly lower expression of the transcript encoding ZNF423 in chondrocytes from OA, particularly in elderly patients. Interestingly, this decrease was mirrored by the similarly reduced expression of PPARγ, a known target of ZNF423 with anti-inflammatory and chondroprotective properties. The ZNF521 mRNA instead was abundant in all primary chondrocytes studied; the RNAi-mediated silencing of this gene significantly altered the COL2A/COL1 expression ratio, associated with the maintenance of the differentiated phenotype, in chondrocytes cultivated in alginate beads. These results suggest a role for ZNF423 and ZNF521 in the regulation of chondrocyte homeostasis and warrant further investigations to elucidate their mechanism of action.http://dx.doi.org/10.1155/2014/318793 |
| spellingShingle | Maria Mesuraca Olimpio Galasso Leonardo Guido Emanuela Chiarella Stefania Scicchitano Renaud Vatrinet Giovanni Morrone Heather M. Bond Giorgio Gasparini Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes Mediators of Inflammation |
| title | Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes |
| title_full | Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes |
| title_fullStr | Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes |
| title_full_unstemmed | Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes |
| title_short | Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes |
| title_sort | expression profiling and functional implications of a set of zinc finger proteins znf423 znf470 znf521 and znf780b in primary osteoarthritic articular chondrocytes |
| url | http://dx.doi.org/10.1155/2014/318793 |
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