Protocol for long-term ex vivo cultivation and imaging of Drosophila imaginal discs
Summary: Drosophila wing and eye-antennal imaginal discs are excellent models for studying tissue development and cell behavior. Here, we present a protocol for long-term cultivation and imaging of wing and eye-antennal imaginal discs. We describe the mounting, dissection, and ex vivo cultivation of...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002825 |
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| Summary: | Summary: Drosophila wing and eye-antennal imaginal discs are excellent models for studying tissue development and cell behavior. Here, we present a protocol for long-term cultivation and imaging of wing and eye-antennal imaginal discs. We describe the mounting, dissection, and ex vivo cultivation of imaginal discs. We further detail 4D time-lapse image acquisition with minimized phototoxicity and image restoration using a machine-learning-based algorithm. This protocol is suitable for studying dynamic cell behaviors with single-cell resolution during normal and perturbed development.For complete details on the use and execution of this protocol, please refer to Sui and Dahmann.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |