An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging

Abstract Bioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo. With recent improvements in BLI brightness from the engineering of bioluminescent enzymes (luciferases) and substrates (luciferins), o...

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Bibliographic Details
Main Authors: Chao Gao, Yan Wu, Connor Fitzgerald, Hui Wang, Tim Ugo, Tetsuo Uyeda, Wenhui Zhou, Yichi Su, Thomas A. Kirkland, Michael Z. Lin
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-97366-9
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