Cloning and expression of African swine fever virus K205R and B602L genes inf. COLI
A molecular cloning of African swine fever virus k205r and b602l genes in £ coli was carried out. The expression and purification conditions ensuring high yield of recombinant proteins were optimized. Dissolved recombinant proteins were purified by metal-chelate affinity chromatography using Ni-NTA-...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2018-04-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/191 |
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| _version_ | 1832086785433272320 |
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| author | A. V. Scherbakov A. S. Yakovleva A. M. Timina M. R. Yakupov |
| author_facet | A. V. Scherbakov A. S. Yakovleva A. M. Timina M. R. Yakupov |
| author_sort | A. V. Scherbakov |
| collection | DOAJ |
| description | A molecular cloning of African swine fever virus k205r and b602l genes in £ coli was carried out. The expression and purification conditions ensuring high yield of recombinant proteins were optimized. Dissolved recombinant proteins were purified by metal-chelate affinity chromatography using Ni-NTA-agarose («Qiagen»). Recombinant antigens are biologically safe, easier to prepare and ensure higher ELISA specificity. The purified protein yield from 100 ml off. coli culture was 1,5 mg for pK205R and 2 mg for pB602L. |
| format | Article |
| id | doaj-art-01a098b701494b52905585290ba7c90d |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-01a098b701494b52905585290ba7c90d2025-02-06T09:52:05ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-01023135191Cloning and expression of African swine fever virus K205R and B602L genes inf. COLIA. V. Scherbakov0A. S. Yakovleva1A. M. Timina2M. R. Yakupov3FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirA molecular cloning of African swine fever virus k205r and b602l genes in £ coli was carried out. The expression and purification conditions ensuring high yield of recombinant proteins were optimized. Dissolved recombinant proteins were purified by metal-chelate affinity chromatography using Ni-NTA-agarose («Qiagen»). Recombinant antigens are biologically safe, easier to prepare and ensure higher ELISA specificity. The purified protein yield from 100 ml off. coli culture was 1,5 mg for pK205R and 2 mg for pB602L.https://veterinary.arriah.ru/jour/article/view/191african swine fever viruspk205r and pb602l recombinant proteinsexpression |
| spellingShingle | A. V. Scherbakov A. S. Yakovleva A. M. Timina M. R. Yakupov Cloning and expression of African swine fever virus K205R and B602L genes inf. COLI Ветеринария сегодня african swine fever virus pk205r and pb602l recombinant proteins expression |
| title | Cloning and expression of African swine fever virus K205R and B602L genes inf. COLI |
| title_full | Cloning and expression of African swine fever virus K205R and B602L genes inf. COLI |
| title_fullStr | Cloning and expression of African swine fever virus K205R and B602L genes inf. COLI |
| title_full_unstemmed | Cloning and expression of African swine fever virus K205R and B602L genes inf. COLI |
| title_short | Cloning and expression of African swine fever virus K205R and B602L genes inf. COLI |
| title_sort | cloning and expression of african swine fever virus k205r and b602l genes inf coli |
| topic | african swine fever virus pk205r and pb602l recombinant proteins expression |
| url | https://veterinary.arriah.ru/jour/article/view/191 |
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