Vitamin D Receptor, Retinoid X Receptor, Ki-67, Survivin, and Ezrin Expression in Canine Osteosarcoma
Canine osteosarcoma (OS) is an aggressive malignant bone tumor. Prognosis is primarily determined by clinical parameters. Vitamin D has been postulated as a novel therapeutic option for many malignancies. Upon activation, vitamin D receptors (VDRs) combine with retinoid receptor (RXR) forming a hete...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2012/761034 |
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| _version_ | 1832561954551496704 |
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| author | John Davies Heather Heeb Rama Garimella Kimberly Templeton David Pinson Ossama Tawfik |
| author_facet | John Davies Heather Heeb Rama Garimella Kimberly Templeton David Pinson Ossama Tawfik |
| author_sort | John Davies |
| collection | DOAJ |
| description | Canine osteosarcoma (OS) is an aggressive malignant bone tumor. Prognosis is primarily determined by clinical parameters. Vitamin D has been postulated as a novel therapeutic option for many malignancies. Upon activation, vitamin D receptors (VDRs) combine with retinoid receptor (RXR) forming a heterodimer initiating a cascade of events. Vitamin D's antineoplastic activity and its mechanism of action in OS remain to be clearly established.
Expression of VDR, RXR, Ki-67, survivin, and ezrin was studied in 33 archived, canine OS specimens. VDR, RXR, survivin, and ezrin were expressed in the majority of cases. There was no statistically significant difference in VDR expression in relationship with tumor grade, type, or locations or animal breed, age, and/or sex. No significant association (p=0.316) between tumor grade and Ki-67 expression was found; in particular, no difference in Ki-67 expression between grades 2 and 3 OSs was found, while a negative correlation was noted between Ki-67 and VDR expression (ρ=−0.466), a positive correlation between survivin and RXR expression was found (p=0.374). A significant relationship exists between VDR and RXR expression in OSs and proliferative/apoptosis markers. These results establish a foundation for elucidating mechanisms by which vitamin D induces antineoplastic activity in OS. |
| format | Article |
| id | doaj-art-017c6715936a4a0b959f4532f3036354 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-017c6715936a4a0b959f4532f30363542025-02-03T01:23:46ZengWileyVeterinary Medicine International2090-81132042-00482012-01-01201210.1155/2012/761034761034Vitamin D Receptor, Retinoid X Receptor, Ki-67, Survivin, and Ezrin Expression in Canine OsteosarcomaJohn Davies0Heather Heeb1Rama Garimella2Kimberly Templeton3David Pinson4Ossama Tawfik5BluePearl Veterinary Partners, Overland Park, KS 66210, USABluePearl Veterinary Partners, Overland Park, KS 66210, USAOncology Division, Department of Medicine, Kansas University Medical Center, Kansas City, KS 66160, USADepartment of Orthopedic Surgery, Kansas University Medical Center, Kansas City, KS 66160, USADepartment of Pathology and Laboratory Medicine, Kansas University Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USADepartment of Pathology and Laboratory Medicine, Kansas University Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USACanine osteosarcoma (OS) is an aggressive malignant bone tumor. Prognosis is primarily determined by clinical parameters. Vitamin D has been postulated as a novel therapeutic option for many malignancies. Upon activation, vitamin D receptors (VDRs) combine with retinoid receptor (RXR) forming a heterodimer initiating a cascade of events. Vitamin D's antineoplastic activity and its mechanism of action in OS remain to be clearly established. Expression of VDR, RXR, Ki-67, survivin, and ezrin was studied in 33 archived, canine OS specimens. VDR, RXR, survivin, and ezrin were expressed in the majority of cases. There was no statistically significant difference in VDR expression in relationship with tumor grade, type, or locations or animal breed, age, and/or sex. No significant association (p=0.316) between tumor grade and Ki-67 expression was found; in particular, no difference in Ki-67 expression between grades 2 and 3 OSs was found, while a negative correlation was noted between Ki-67 and VDR expression (ρ=−0.466), a positive correlation between survivin and RXR expression was found (p=0.374). A significant relationship exists between VDR and RXR expression in OSs and proliferative/apoptosis markers. These results establish a foundation for elucidating mechanisms by which vitamin D induces antineoplastic activity in OS.http://dx.doi.org/10.1155/2012/761034 |
| spellingShingle | John Davies Heather Heeb Rama Garimella Kimberly Templeton David Pinson Ossama Tawfik Vitamin D Receptor, Retinoid X Receptor, Ki-67, Survivin, and Ezrin Expression in Canine Osteosarcoma Veterinary Medicine International |
| title | Vitamin D Receptor, Retinoid X Receptor, Ki-67, Survivin, and Ezrin Expression in Canine Osteosarcoma |
| title_full | Vitamin D Receptor, Retinoid X Receptor, Ki-67, Survivin, and Ezrin Expression in Canine Osteosarcoma |
| title_fullStr | Vitamin D Receptor, Retinoid X Receptor, Ki-67, Survivin, and Ezrin Expression in Canine Osteosarcoma |
| title_full_unstemmed | Vitamin D Receptor, Retinoid X Receptor, Ki-67, Survivin, and Ezrin Expression in Canine Osteosarcoma |
| title_short | Vitamin D Receptor, Retinoid X Receptor, Ki-67, Survivin, and Ezrin Expression in Canine Osteosarcoma |
| title_sort | vitamin d receptor retinoid x receptor ki 67 survivin and ezrin expression in canine osteosarcoma |
| url | http://dx.doi.org/10.1155/2012/761034 |
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