A novel Arthrobotrys species: Taxonomic characterization, nematicidal activity, and multi-omics insights into nematode predation by Mengting Gao, Zhaoqi Yan, Zexin Liu, Yunxia Jiang, Tengteng Liu, Xingjun Miao, Meixue Dai, Tanay Bose, Runlei Chang

“…During a survey of bark beetle-associated fungi, a nematode-trapping fungus was isolated from an empty beetle gallery in Pinus thunbergii. ITS sequence analysis suggested it represented a novel species. …”
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Effects of tryptamine on duckweed growth by Qiqi Di, Wenqian Han, Yujie Han, Sizheng Liu, Yi Hu, Ziyang Qu, Yumeng Jiang, Weibo Sun, Ting Qiu, Lin Yang

“…Moreover, transcriptomic analysis revealed an upregulation of auxin (IAA) metabolism-related enzymes by Try addition. …”
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A spatiotemporal atlas of mouse gastrulation and early organogenesis to explore axial patterning and project in vitro models onto in vivo space by Luke T.G. Harland, Tim Lohoff, Noushin Koulena, Nico Pierson, Constantin Pape, Farhan Ameen, Jonathan Griffiths, Bart Theeuwes, Nicola K. Wilson, Anna Kreshuk, Wolf Reik, Jennifer Nichols, Long Cai, John C. Marioni, Berthold Göttgens, Shila Ghazanfar

“…Summary: During gastrulation, mouse epiblast cells form the three germ layers that establish the body plan and initiate organogenesis. While single-cell atlases have advanced our understanding of lineage diversification, spatial aspects of differentiation remain poorly defined. …”
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Gibberellin-3 induced dormancy and suppression of flower bud formation in pitaya (Hylocereus polyrhizus) by Kamran Shah, Xiaoyue Zhu, Tiantian Zhang, Jiayi Chen, Jiaxuan Chen, Yonghua Qin

“…Conversely, FB formation was associated with increased levels of AUX, dhZR, ZR, iPA, ABA, and JA, and decreased GA3 and GA4 levels. Transcriptomic analysis revealed batches of differentially expressed genes (DEGs) associated with phytohormone signal transduction, aligning with observed hormone changes. …”
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Exploring the role of TIGIT in patients with Small Cell Lung Cancer as a novel predictor of prognosis and immunotherapy response by Li Liu, Peng Wu, Bingzhi Wang, Jiyan Dong, Chaoqi Zhang, Wenchao Liu, Jianming Ying

“…Immune infiltration analysis revealed a strong positive correlation between TIGIT expression and immune score in multiple cohorts. …”
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Bioprinted Patient‐Derived Organoid Arrays Capture Intrinsic and Extrinsic Tumor Features for Advanced Personalized Medicine by Jonghyeuk Han, Hye‐Jin Jeong, Jeonghan Choi, Hyeonseo Kim, Taejoon Kwon, Kyungjae Myung, Kyemyung Park, Jung In Park, Samuel Sánchez, Deok‐Beom Jung, Chang Sik Yu, In Ho Song, Jin‐Hyung Shim, Seung‐Jae Myung, Hyun‐Wook Kang, Tae‐Eun Park

“…This model faithfully reproduces critical TME attributes, including elevated matrix stiffness (≈7.5 kPa) and hypoxic conditions found in CRC. Transcriptomic and immunofluorescence microscopy analysis reveal that Eba‐PDOs more accurately represent actual tissues compared to traditional PDOs. …”
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SENP1-Sirt3 axis regulates type II alveolar epithelial cell activity to confer resistance against oxidative damage in lung tissue by Mingming Zhang, Xin Lin, Jianli He, Yong Zuo, Qiuju Fan, Innocent Agida, Hongsheng Tan, Caiying Zhu, Jinke Cheng, Tianshi Wang

“…Additionally, SENP1-Sirt3 activation attenuated inflammation and fibrosis in lung tissue. Transcriptomic analysis linked the axis to enhanced Wnt signaling and lipid metabolism pathways, promoting AT2 stemness. …”
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Emerging biomarkers in amyotrophic lateral sclerosis: from pathogenesis to clinical applications by Farah Anjum, Farah Anjum, Maha Bakhuraysah, Maha Bakhuraysah, Abdulaziz Alsharif, Abdulaziz Alsharif, Taj Mohammad, Anas Shamsi, Md. Imtaiyaz Hassan

“…Current biomarker detection methods, such as invasive cerebrospinal fluid (CSF) analysis or advanced imaging, are impractical for routine use, particularly in late-stage ALS. …”
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Serum LEAP2 Levels Across the Spectrum of Metabolic Dysfunction-Associated Fatty Liver Disease: A Potential Noninvasive Biomarker for Severity Stratification by Huang X, Deng Z, Li X, Yan S, Zhong K, Yuan F, Liu L, Deng C, Liu T, Zhao R, Buhe A, Li T, Zhao H

“…The diagnostic performance of LEAP2 was evaluated through receiver operating characteristic (ROC) curve analysis. Additionally, Hepatic LEAP2 transcriptomic data from public Gene Expression Omnibus (GEO) datasets (GSE126848, GSE135251) were analyzed for validation.Results: Seventy-four participants (24 HC, 24 SS, 26 MASH) were analyzed. …”
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DNA methylation as a contributor to dysregulation of STX6 and other frontotemporal Lobar degeneration genetic risk-associated loci by Naiomi Rambarack, Katherine Fodder, Megha Murthy, Christina Toomey, Rohan de Silva, Peter Heutink, Jack Humphrey, Towfique Raj, Tammaryn Lashley, Conceição Bettencourt

“…We then leveraged FTLD transcriptomic and proteomic datasets to investigate possible downstream effects of DNA methylation changes. …”
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Potential physiological mechanisms behind variation in rainbow trout (Oncorhynchus mykiss) to biosynthesize EPA and DHA when reared on plant oil replacement feeds by Ken Overturf, Jason Abernathy, Dietmar Kültz, Jacob Bledsoe, Shawn Narum, Thomas Welker

“…The high performing group averaged 8.86 % for EPA+DHA muscle fatty acid ratios, the average group was 6.33 %, and the low performing group was 4.84 %. Transcriptomic and proteomic analysis of liver and muscle tissue was used to evaluate differences between these groups to identify potential mechanisms responsible for the trait. …”
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CPT1A/HIF-1α positive feedback loop induced fatty acid oxidation metabolic pathway contributes to the L-ascorbic acid-driven angiogenesis in breast cancer by Xiao Ma, Baojian Zhang, Xuezhe Yin, ShiPeng Yang, Zhenhua Lin, Yang Yang, Xianchun Zhou

“…Following this, the reciprocal regulatory relationship between CPT1A and HIF-1α was elucidated using transcriptomic studies, ubiquitination analysis, and dual-luciferase assays. …”
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Adaptation to Arginine Deprivation Leads to a More Aggressive, Therapy-Resistant Phenotype in HNSCC Cells by Oleg Chen, Olena Vovk, Nikita Polishchuk, Oksana Mayevska, Galyna Shuvayeva, Melike Demir, Vasyl Lukiyanchuk, Leoni A. Kunz-Schughart, Anna Dubrovska, Oleh Stasyk

“…This subline was compared to the parental SAS cell lines for its relative clonogenic proliferation, aggregation, adhesion, and migration capacities. The transcriptomic changes were assessed by RNA sequencing. …”
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Ex vivo-generated lymphoid progenitors encompass both T cell and innate lymphoid cell fates by Pierre Gaudeaux, Pierre Gaudeaux, Juliette Paillet, Juliette Paillet, Monah Abou Alezz, Ranjita Devi Moirangthem, Ranjita Devi Moirangthem, Sara Cascione, Marta Martin Corredera, Marta Martin Corredera, Anne-Catherine Dolens, Anne-Catherine Dolens, Katrien De Mulder, Katrien De Mulder, Imke Velghe, Imke Velghe, Bart Vandekerckhove, Bart Vandekerckhove, Marieke Lavaert, Marieke Lavaert, Noémie Robil, Aurélien Corneau, Aurélien Corneau, Hanem Sadek, Pauline Rault, Akshay Joshi, Pierre de la Grange, Frank J. T. Staal, Frank J. T. Staal, Tom Taghon, Tom Taghon, Olivier Negre, Andrea Ditadi, Isabelle André, Tayebeh-Shabi Soheili

“…In vivo studies demonstrated that adoptive transfer of these progenitors accelerates T cell reconstitution following thymic engraftment.MethodTo further explore the full therapeutic potential of this cell product, we performed a comprehensive molecular and phenotypic characterization using single cell RNA sequencing and mass cytometry analysis.ResultsOur analysis revealed the presence of distinct cell subsets within the cellular product characterized mainly by commitment to lymphoid lineages. …”
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Cancer-associated foam cells hamper protective T cell immunity and favor tumor progression in human colon carcinogenesis by Barbara Vergani, Massimo Milione, Marco Vitellaro, Giulio Ferrero, Veronica Huber, Luca Lalli, Agata Cova, Paola Frati, Flavio Arienti, Licia Rivoltini, Simonetta Guarrera, Laura Cattaneo, Luca Sorrentino, Elena Daveri, Elena Casiraghi, Marta Zorza, Manuela Gariboldi, Patrizia Pasanisi, Daniele Morelli, Paola A Corsetto, Angela M Rizzo, Martina Stroscia, Vincenzo Lagano, Giovanna Sabella, Biagio E Leone

“…Multiparametric high-resolution flow cytometry analysis and bulk RNAseq of CRC tissue were conducted to evaluate the phenotype and transcriptomic program of immune cell infiltrate in relation to FC accumulation. …”
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Perilla frutescens Leaf-Derived Extracellular Vesicle-Like Particles Carry Pab-miR-396a-5p to Alleviate Psoriasis by Modulating IL-17 Signaling by Yali Liu, Shanmin Tao, Zhengwei Zhang, Tianjiao Li, Haoran Wang, Jiankang Mu, Yunke Wu, Ziheng He, Cheng Zhang, Dominique Jasmin Lunter, Peng Cao

“…This effect was attributed to reduced oxidative stress, modulation of Treg cells, and promotion of keratinocyte apoptosis. Transcriptomic analysis revealed enrichment of the interleukin-17 (IL-17) signaling pathway, a major driver of psoriasis, while small RNA sequencing identified pab-miR396a-5p, an endogenous microRNA (miRNA) within PLEVPs, as a key regulator. …”
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Integrative spatial omics reveals distinct tumor-promoting multicellular niches and immunosuppressive mechanisms in Black American and White American patients with TNBC by Qian Zhu, Akhila Balasubramanian, Jaya Ruth Asirvatham, Megha Chatterjee, Badrajee Piyarathna, Jaspreet Kaur, Nada Mohamed, Ling Wu, Stacy Wang, Niloufar Pourfarrokh, Paula Danika Binsol, Mahak Bhargava, Uttam Rasaily, Yitian Xu, Junjun Zheng, Deborah Jebakumar, Arundhati Rao, Carolina Gutierrez, Angela R. Omilian, Carl Morrison, Gokul M. Das, Christine Ambrosone, Erin H. Seeley, Shu-hsia Chen, Yi Li, Eric Chang, Xiaoxian Li, Elizabeth Baker, Ritu Aneja, Xiang H.-F. Zhang, Arun Sreekumar

“…Our analysis revealed that the TME in BA patients is marked by a network of endothelial cells, macrophages, and mesenchymal-like cells, which correlates with reduced patient survival. …”
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Micheliolide Alleviates Hepatic Fibrosis by Inhibiting Autophagy in Hepatic Stellate Cells via the TrxR1/2-Mediated ROS/MEK/ERK Pathway by Yi Liu, Ling Yao, Yuanyuan Liu, Yunheng Yang, Ailing Liang, Honglin He, Yao Lei, Wenfu Cao, Zhiwei Chen

“…Additionally, MCL significantly inhibited LX-2 cell activation by suppressing cell proliferation, extracellular matrix (ECM) production, and autophagy, while activating the MEK/ERK pathway. Moreover, MCL elevated intracellular and mitochondrial reactive oxygen species (ROS) levels, reduced mitochondrial membrane potential, and altered mitochondrial morphology. …”
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Fibrocartilage repair involves chronic cellular senescence in a rat model of bone marrow stimulation by Luke Childress, Landon B. Gatrell, Hong Wu, Elisabeth Ferreira, Intawat Nookaew, Ryan M. Porter

“…Repair tissue was characterized during the first months after marrow stimulation by histology as well as bulk and single cell transcriptomic analysis. Results: Multiple senescence markers were measured within repair tissue during the first months of repair, in contrast to adjacent, intact articular cartilage. …”
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Stereotyped Subclones Revealed by High‐Density Single‐Cell Lineage Tracing Support Robust Development by Xiaoyu Zhang, Zizhang Li, Jingyu Chen, Wenjing Yang, Xingxing He, Peng Wu, Feng Chen, Ziwei Zhou, Chenze Ren, Yuyan Shan, Xiewen Wen, Vassily A. Lyubetsky, Leonid Yu. Rusin, Xiaoshu Chen, Jian‐Rong Yang

“…This question is approached using a well‐established in vitro directed differentiation model recapitulating the in vivo development of lung progenitor cells from human embryonic stem cells. An integrated analysis of high‐density cell lineage trees (CLTs) and single‐cell transcriptomes of differentiating colonies enabled the resolution of known cell types and developmental hierarchies. …”
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