Orf132: a critical gene for LSDV replication and its role in Er stress-related apoptosis by Yongtao Wang, Shiwei Zhang, Hailing Li, Yali Feng, Ying Zhang

“…Compared with the wild-type LSDV, the replication capacity of LSDV-ΔOrf132 was reduced approximately tenfold, indicating that Orf132 is critical for viral replication. Transcriptomic analysis of infected MDBK cells revealed significant alterations in endoplasmic reticulum (ER) protein processing and unfolded protein response (UPR) pathways following Orf132 deletion. qRT-PCR validation showed marked upregulation of ER stress markers, including Grp78, Chop, and Gadd34. …”
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Astragaloside IV accelerates hematopoietic reconstruction by improving the AMPK/PGC1α-mediated mitochondrial function in hematopoietic stem cells by Ling Zhang, Wanqi Xu, Yueying Zeng, Long Wang, Jiesi Luo, Xiaogang Zhou, Qibing Mei, Dalian Qin, Anguo Wu, Jianming Wu, Feihong Huang

“…Further investigation revealed that AS-IV increased the proportion of HSC in both bone marrow and spleen while improving their mitochondrial function. Transcriptomic sequencing and Western blot analysis identified the AMPK/PGC1α signaling pathway as the key mechanism underlying AS-IV-mediated mitochondrial enhancement. …”
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Time-resolved oxidative signal convergence across the algae–embryophyte divide by Tim P. Rieseberg, Armin Dadras, Tatyana Darienko, Sina Post, Cornelia Herrfurth, Janine M. R. Fürst-Jansen, Nils Hohnhorst, Romy Petroll, Stefan A. Rensing, Thomas Pröschold, Sophie de Vries, Iker Irisarri, Ivo Feussner, Jan de Vries

“…Stress on land is unique in its dynamics, entailing swift and drastic changes in light and temperature. While we know that land plants share with their closest streptophyte algal relatives key components of the genetic makeup for dynamic stress responses, their concerted action is little understood. …”
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Epigenetic insights into physiological resilience: Multigenerational readouts of CO2-induced seawater acidification effects on fish embryos by Tzu-Yen Liu, Jia-Jiun Yan, Ying-Jey Guh, Oki Hayasaka, Li-Yih Lin, Pung-Pung Hwang, Guan-Chung Wu, Ming-Tsung Chung, Yung-Che Tseng

“…Metabolic responses were generation-specific: F0 embryos displayed decreased ammonium excretion, while F1 and F2 maintained stable profiles. Transcriptomic analysis revealed generational changes in neurotransmission, ion regulation, and epigenetic pathways. …”
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Genomic subtypes of non-muscle-invasive bladder cancer: guiding immunotherapy decision-making for patients exposed to aristolochic acid by Yun Peng, Yuxuan Song, Caipeng Qin, Mengting Ding, Zixiong Huang, Fei Wang, Yuchao HuangFu, Luping Yu, Yiqing Du, Tao Xu

“…We performed whole-exome sequencing (WES) and RNA sequencing (RNA-seq) to obtain detailed genomic and transcriptomic data. We subjected these datasets to genomic analysis and subtype analysis to gain valuable insights into NMIBC. …”
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Saikosaponin A Mediates the Anti-Acute Myeloid Leukemia Effect via the P-JNK Signaling Pathway Induced by Endoplasmic Reticulum Stress by Sun XH, Chai YH, Bai XT, Li HX, Yang PP, Xi YM

“…RNA sequencing was performed to identify differentially expressed genes and enriched signaling pathways. Western blot analysis was utilized to confirm the involvement of ERS and activation of the MAPK-p-JNK signaling pathway. …”
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Single-cell characterization of the immune heterogeneity of pulmonary hypertension identifies novel targets for immunotherapy by Pan Jiang, Huai Huang, Mengshi Xie, Zilong Liu, Lijing Jiang, Hongyu Shi, Xiaodan Wu, Shengyu Hao, Shanqun Li

“…We presented an in-depth analysis of T cell subsets, myeloid cell heterogeneity and a comprehensive analysis of SMCs and FBs subsets in PAH. …”
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Oil trait and multi-omic analyses reveal the regulatory network of triacylglycerol and fatty acid accumulation in hexaploid Camellia oleifera across different harvesting stages by Jugang Wang, Jian Song, Xiaomin Gao, Jiali Wang, Tianyu Huang, Manmei Zeng, Zhoujun Zhu, Min Liu, Zuhua Wang

“…Results Integrated analysis of oil traits and multi-omics data demonstrated that premature harvesting (1 week before the recommended date) decreased oil production and enhanced saturated FAs composition, while postponed harvesting (1 week after the recommended date) preserved oil yield and increased oleic and linolenic acid contents. …”
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Causal relationships between alterations in shear stress-related genes and aneurysmal subarachnoid hemorrhage by Xiaoxin Wu, Yuanyuan Dai, Xingyang Niu, Minghao Zhang, Jiaoxing Li, Yi Xie, Wenli Sheng, Fei Ye

“…Functional experiments using siRNA-mediated knockdown in HUVECs showed that siKCNN4 increased cell proliferation and disrupted endothelial barriers, while siUGCG enhanced tube formation ability but reduced migration. …”
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Screened of long non-coding RNA related to wool development and fineness in Gansu alpine fine-wool sheep by Zhaohua He, Fangfang Zhao, Hongxian Sun, Jiang Hu, Jiqing Wang, Xiu Liu, Mingna Li, Zhiyun Hao, Zhidong Zhao, Bingang Shi, Feiyan Liu, Shaobin Li

“…Furthermore, a comprehensive analysis revealed 164 differentially expressed lncRNAs along with 146 potential target genes linked to these lncRNAs in the skin tissues from groups C and F. …”
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Brown adipose tissue-derived extracellular vesicles regulate hepatocyte mitochondrial activity to alleviate high-fat diet-induced jawbone osteoporosis in mice by Kai Zhang, Kai Zhang, Sha Zhang, Sha Zhang, Sha Zhang, Guorong Deng, Guangxiang He, Guangxiang He, Yuan Yuan, Yu Fu, Yihan Liu, Zhen Gong, Liang Kong, Chenxi Zheng

“…The use of metabolic regulators represents a promising therapeutic strategy for OP management. While brown adipose tissue-derived extracellular vesicles (BEV) exhibit metabolic regulatory potential, their capacity to mitigate LMD-associated OP remains unclear.MethodsA high-fat diet (HFD)-induced LMD mouse model was established to identify the JOP phenotype through micro-computed tomography (micro-CT) and transcriptomic profiling. …”
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Exploring therapeutic paradigm focusing on genes, proteins, and pathways to combat leprosy and tuberculosis: A network medicine and drug repurposing approach by Mohd Imran, Ahmed S. Alshrari, Mariah N. Hafiz, Mohammed M. Jawad, Abida Khan, Fadiyah Jadid Alanazi, Syed Mohammed Basheeruddin Asdaq

“…This study integrates transcriptomic analysis cheminformatics and molecular dynamics simulations to identify molecular mechanisms and potential therapeutic targets. …”
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70% ethanol preserves mycobacterial RNA from cultures more efficiently than GTC-TCEP by Lena Krausser, Magalie Van Dyck-Lippens, Ramata Balde, Rabab Reenaers, Reem Al Mubarak, Leen Rigouts, Nicholas D. Walter, Martin I. Voskuil, Annelies Van Rie, Bouke C. de Jong, Sofie M. Braet

“…Based on these results, we recommend 70% ethanol over GTC-TCEP for RNA preservation of mycobacterial cultures. While freeze-thawing, short-term high-temperature and − 20 °C long-term storage results are promising, this inexpensive, widely available buffer needs further validation prior to applying it for RNA-based analysis in clinical samples.…”
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Polysaccharides derived from Rosa rugosa cv. Plena ameliorate colorectal cancer by regulating intestinal microbiota composition and lipid metabolism pathway by Chunyu Dai, Xuan Zheng, Jingxin Zhu, Hongxing Zhang, Junhua Jin, Xiaona Pang, Xiangyang Li, Yuanhong Xie

“…In CRC mice, RPP1 administration reduced colonic polyp formation and ameliorated colon shortening while normalizing elevated serum IL-1β and TNF-α levels. …”
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Fungal Coculture: Unlocking the Potential for Efficient Bioconversion of Lignocellulosic Biomass by Rafael Icaro Matos Vieira, Alencar da Silva Peixoto, Antonielle Vieira Monclaro, Carlos André Ornelas Ricart, Edivaldo Ximenes Ferreira Filho, Robert Neil Gerard Miller, Taísa Godoy Gomes

“…Recent advancements in -omics technologies, including genomics, transcriptomics, and proteomics, have facilitated detailed analysis of fungal metabolism, uncovering novel secondary metabolites and enzymatic pathways activated under specific growth conditions. …”
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Distinct microRNA signatures define sporadic PSP-RS and PD in patient-derived midbrain organoids by Desirèe Valente, Clara Zannino, Stefania Scalise, Davide Bressan, Valeria Lucchino, Ermes Filomena, Giorgia Lucia Benedetto, Ida Lazzinnaro, Mariagrazia Talarico, Andrea Quattrone, Fulvio Chiacchiera, Graziano Pesole, Aldo Quattrone, Elvira Immacolata Parrotta, Giovanni Cuda

“…These 3D organoids recapitulated disease-relevant pathologies, including tau hyperphosphorylation in PSP-RS and α-synuclein aggregation in PD. Transcriptomic analysis revealed dynamic, disease-specific miRNA signatures. …”
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Novel Hyperplastic Expansion of White Adipose Tissue Underlies the Metabolically Healthy Obese Phenotype of Male LFABP Null Mice by Anastasia Diolintzi, Yinxiu Zhou, Angelina Fomina, Yifei Sun, Seema Husain, Labros S. Sidossis, Susan K. Fried, Judith Storch

“…Transcriptomic analysis of SAT revealed that <i>Lfabp</i> deletion alters the expression of multiple pathways that modulate adipose expansion and function including cholesterol biosynthesis, adipogenesis, and extracellular matrix remodeling. …”
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A KIF20A-based thermosensitive hydrogel vaccine effectively potentiates immune checkpoint blockade therapy for hepatocellular carcinoma by Xingyang Zhao, Feichao Xuan, Zirong Li, Xiangyi Yin, Xiaojun Zeng, Jiali Chen, Chihua Fang

“…This study confirmed KIF20A as a promising immunogenic antigen through transcriptomic mRNA sequencing analysis in the context of HCC. …”
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Divergent B-cell and cytotoxic TNK cell activation signatures in HLA-B27-associated ankylosing spondylitis and acute anterior uveitis by Eisa Mahyari, Sean Davin, Kimberly Ogle, Emma Fale-Olsen, Carley Shaut, Tammy M. Martin, Jasvinder S. Ahuja, Eric Suhler, Atul Deodhar, James T. Rosenbaum, James T. Rosenbaum, Tejpal Gill, Tejpal Gill

“…Using cellular indexing of transcriptomes and epitopes (CITE-Seq) in a well-characterized cohort of 25 subjects—including AS (HLA-B27pos), AS+AAU (HLA-B27pos), AAU (HLA-B27pos), HCs (HLA-B27pos), and HCs (HLA-B27neg); N = 5/group—we identified transcriptomic differences at the single-cell level, along with differentially expressed cell surface markers. …”
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Pretreated exosomes by electrical stimulation accelerate bone regeneration by Jialu Chen, Jian Chen, Jiahao Chen, Renjie Lu, Ziyuan Liu, Yang Zhang, Chi Zhang

“…Mechanistically, proteomic analysis revealed an enrichment of proteins involved in “Oxidative Phosphorylation” regulation within Elec-exo, and transcriptomic analysis indicated the activation of Pl3k-Akt and MAPK bone formation-related signaling pathways in the effector cells. …”
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