<i>Tn</i>P and AHR-CYP1A1 Signaling Crosstalk in an Injury-Induced Zebrafish Inflammation Model by Geonildo Rodrigo Disner, Thales Alves de Melo Fernandes, Milton Yutaka Nishiyama-Jr, Carla Lima, Emma Wincent, Monica Lopes-Ferreira

“…This study aimed to elucidate <i>Tn</i>P’s molecular mechanisms on the AHR–cytochrome P450, family 1 (CYP1) pathway upon injury-induced inflammation in wild-type (WT) and <i>Ahr2</i>-knockdown (KD) zebrafish larvae through transcriptomic analysis and <i>Cyp1a</i> reporters. <i>Tn</i>P, while unable to directly activate AHR, potentiated AHR activation by the high-affinity ligand 6-Formylindolo [3,2-b]carbazole (FICZ), implying a role as a CYP1A inhibitor, confirmed by in vitro studies. …”
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The Effect of <i>G0S2</i> Gene Knockout on the Proliferation, Apoptosis, and Differentiation of Chicken Preadipocytes by Yantao Li, Boyu Wang, Zhaochuan Wang, Jintian Wen, Tianle Zhou, Jiahao Tang, Zhenhui Li

“…<i>G0S2</i> deletion significantly affected the expression of important genes related to lipid metabolism, cell cycle control, and signaling pathways, based on transcriptomic analysis. In conclusion, our findings suggest that <i>G0S2</i> performs a critical role in regulating chicken preadipocyte differentiation, proliferation, and apoptosis. …”
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Molecular and Biological Characterization of Novel Mitovirus Infecting Phytopathogenic Fungus Ustilaginoidea virens by He Zhenrui, Zhao Wenhua, Cheng Baoping, Yang Mei, Yang Yingqing, Zhu Yiming, Zhou Erxun

“…Activation or inhibition of autophagy through chemical experiments demonstrated that UvMV1 enhances viral titers by inducing autophagy in U. virens, while inhibition of autophagy results in decreased UvMV1 titers. …”
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Epigenetic modifier alpha-ketoglutarate modulates aberrant gene body methylation and hydroxymethylation marks in diabetic heart by Rohini Dhat, Dattatray Mongad, Sivarupa Raji, Silpa Arkat, Nitish R. Mahapatra, Nishant Singhal, Sandhya L. Sitasawad

“…Hyperglycemic myocardial microenvironment significantly alters chromatin architecture and the transcriptome, resulting in aberrant activation of signaling pathways in a diabetic heart. …”
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Effect of m⁶A Recognition Protein YTHDC1 on Skeletal Muscle Growth by Huijun Huang, Geyan Lu, Liyao Xiao, Baohua Tan, Yuming Yang, Linjun Hong, Zicong Li, Gengyuan Cai, Ting Gu

“…Furthermore, the transcriptome sequencing analysis revealed that YTHDC1 might modulate skeletal muscle development by regulating alternative splicing of genes, including <i>Akap13</i>, <i>Smarca2</i>, <i>Tnnt3</i>, and <i>Neb</i>. …”
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Study on the Regulatory Mechanisms of Carapace Marking Formation in <i>Marsupenaeus japonicus</i> by Panpan Wang, Jiawei Zhu, Huanyu Chen, Qingyuan Hu, Zhenxiang Chen, Wenjia Li, Ting Yang, Jin Zhu, Binlun Yan, Huan Gao, Chaofan Xing

“…In a previous study, we used comparative transcriptome technology to screen some functional genes related to body color regulation. …”
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FimC binds to the promoter region of agn43 to modulate autoaggregation by Zhihao Wang, Xiangpeng Niu, Ningyuan Zhong, Lanfang Kong, Saqib Nawaz, Haiyang Zhang, Wei Jiang, Yuanyuan Liu, Jian Tu, Xiangan Han

“…Transcriptomic analysis showed that the deletion of fimC caused significant changes in the gene transcription levels in a variety of pathways, such as flagellar synthesis, biofilm formation, quorum sensing, and bis-(3′-5′)-cyclic diguanylic acid (c-di-GMP) metabolism. …”
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An ovarian insulin-like peptide specifically regulates energy allocation and oocyte development in nutrition-restricted cockroaches by Yuan Luo, Wen-Xin Hou, Shan-Shan Zhao, Yun-Long Cheng, Ke-Jia Zhang, Liang-Guan Lin, Sheng Li, Xiao-Jin Pei

“…Results In this study, we demonstrate that the ovary plays a crucial role in promoting vitellogenin biosynthesis and oocyte development under nutrition-restricted conditions through hemiovariectomy. Employing transcriptome analysis, RNAi screening, and fluorescence in situ hybridization, we identified an ovarian-enriched insulin-like peptide gene (BgILP2) that is dramatically upregulated during low nutrition conditions. …”
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Zinc sulfate improves insulin resistance, oxidative stress and apoptosis in liver tissues of PCOS rats through the NF-κB pathway by Yi-fan Kang, Jia-yi Zhao, Jian-rong Liu

“…Furthermore, zinc sulfate alleviated severe hepatocellular apoptosis and mitochondrial damage observed in PCOS rats. Transcriptomic analysis indicated that zinc sulfate primarily mitigated PCOS-related liver damage via the cholesterol synthesis pathway, and experimental validation demonstrated that zinc sulfate inhibited oxidative stress and apoptosis in liver cells through the NF-κB pathway.ConclusionOur study demonstrates that zinc sulfate ameliorates liver oxidative stress and apoptosis in PCOS by modulating the NF-κB pathway, offering a novel therapeutic approach for managing PCOS-associated liver diseases.…”
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GWAS and RNA-seq reveal novel loci and genes of low-nitrogen tolerance in cucumber (Cucumis sativus L.) by Huaxiang Wu, Huiming Yan, Bowen Li, Yike Han, Yike Han, Nan Liu, Mengyu Fan, Yahan Liu, Mingjie Lyu, Shengli Du, Shengli Du, Shengli Du, Aimin Wei, Aimin Wei

“…Through GWAS, we identified 29 QTNs harboring 196 candidate genes, while RNA sequencing (RNA-seq) revealed 3,765 differentially expressed genes (DEGs). 24 were identified by both methods. …”
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qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (<i>Litsea coreana</i>) by Qianli Dai, Min Lu, Ximeng Yang, Chenggong Lei, Feiyi Huang, Xueping Hu, Xin Huang, Xiaolong Nie, Daojing Chen, Sicheng Huang, Hengxing Zhu

“…To identify stable reference genes for qRT-PCR analysis across different developmental stages and color variations of tender leaves in <i>Litsea coreana</i>, seven candidate reference genes were selected based on existing transcriptome data. qRT-PCR was performed on tender leaves of <i>L. coreana</i> at various stages and under different color conditions. …”
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Differential regulation of calcium-NFAT signaling pathway by Akt isoforms: unraveling effector dynamics and exhaustion of cytotoxic T lymphocytes in tumor microenvironment by Hung-Chih Yang, Li-Rung Huang, Ulrike Protzer, Wen-Ling Chen, Yong-Lin Chang, Su-Fang Lin, Masanori Isogawa

“…We subsequently conducted western blotting, flow cytometry, and RNA sequencing analysis to assess their Akt expression, expression of immune checkpoints, antitumor/antivirus functionalities, and transcriptome. …”
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Branched-chain amino acids induce hyperammonemia via gut-liver axis-mediated ammonia overproduction in laying hens by Huafeng Jian, Mengru Xu, Fang Liu, Libo Zhang, Qiuming Huang, Jiangang Ma, Xiaoting Zou, Xinyang Dong

“…Compared with Ctrl AA, serum arginine was dramatically decreased by High BCAA (P = 0.005), whereas serum ammonia level was significantly elevated by High Ile, High Leu + Ile, and High BCAA (P = 0.002). Hepatic transcriptome analysis found that the expression of SLC7A2, CPS1, and ARG2 were downregulated in High BCAA group (P < 0.05), which was accompanied by inhibition of the urea cycle and upregulation of SCAP expression (P = 0.024) compared with Ctrl AA. …”
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Construction of a stromal cell-related prognostic signature based on a 101-combination machine learning framework for predicting prognosis and immunotherapy response in triple-nega... by Fanrong Li, Congnan Jin, Yacheng Pan, Zheng Zhang, Liying Wang, Jieqiong Deng, Yifeng Zhou, Yifeng Zhou, Binbin Guo, Shenghua Zhang, Shenghua Zhang

“…Cell types preferentially distributed in TNBC were identified using odds ratios (OR). Bulk transcriptome data were analyzed using Weighted correlation network analysis (WGCNA) to identify myCAF-, VSMC-, and Pericyte-related genes (MVPRGs). …”
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Synthesis of biosafe spirulina-derived carbon dots and their fluorescence imaging in zebrafish by Yue Wang, Zhizhi Gu, Jingyi Dong, Cunguang Liu, Jie Zhu, Zixu Wang, Jiaying Li, Meichen Lu, Jian Han, Shengnan Cao, Zhuang Xue, Yan Chen, Liyong Chen, Wei Wang

“…In adult zebrafish, distinct fluorescent signals are observed in the eye, nerve, and intestinal tissues. Transcriptome analysis revealed that CDs modulated metabolic pathways and extracellular matrix organization without inducing significant toxicity. …”
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Assessing the effects of imidacloprid exposure on the microbiota of Binodoxys communis (Hymenoptera: Braconidae) and gene expression by Qiqing Yu, Weijiao Liu, Likang Zhao, Xiangzhen Zhu, Punniyakotti Elumalai, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Liang Jin, Junyu Luo, Jinjie Cui, Xueke Gao

“…Following treatment with imidacloprid, transcriptomic analysis identified 1263 significantly differentially expressed genes. …”
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Expression characteristics and functional identification of PpD2 gene in peach (Prunus persica) by XIE Hefang, ZHANG Yajia, ZHANG Junjie, LIAN Xiaodong, ZHANG Haipeng, CHENG Jun, WANG Wei, HOU Nan, WANG Lei, ZHENG Xianbo, FENG Jiancan, WANG Xiaobei, TAN Bin

“…[Results] The results revealed that the annual Okubo exhibited a significantly larger branch angle and higher endogenous BR content in axillary buds compared to Sahonglongzhu. Transcriptome analysis showed that the expression of the BR synthesis gene PpD2 was significantly higher in Okubo than in Sahonglongzhu. …”
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Modulation of virulence and metabolic profiles in Klebsiella pneumoniae under indole-mediated stress response by Xueyao Fang, Yanhui Chen, Longhua Hu, Shumin Gu, Junqi Zhu, Yaping Hang, Xingwei Cao, Yanping Xiao, Hong Luo, Chuwen Zhao, Lianhua Xiao, Qiaoshi Zhong

“…To elucidate the underlying molecular mechanisms, transcriptome analysis revealed that indole exposure in K. pneumoniae led to the upregulation of genes associated with pili formation and iron acquisition systems, while simultaneously inducing oxidative stress responses. …”
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Multigeneration Sublethal Chlorantraniliprole Treatment Disrupts Nutritional Metabolism and Inhibits Growth, Development, and Reproduction of <i>Phthorimaea absoluta</i> by Lun Li, Zunzun Jia, Kaiyun Fu, Xinhua Ding, Weihua Jiang, Xiaowu Wang, Tursun. Ahmat, Jiahe Wu, Yutong Wen, Xiaoqin Ye, Wenchao Guo, Hongying Hu

“…The age-stage, two-sex life table analysis revealed that the preadult development time and mean generation time were significantly prolonged, while population reproduction and pupal weight were reduced. …”
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IL-6/GATA2/SERPINE1 pathway is implicated in regulating cellular senescence after acute kidney injury by Hongshuang Su, Xiaoxi Lin, Ayinuer Paredong, Ayinuer Paredong, Congcong Yao, Yan Zhang, Mengke Geng, Yuqian Guan, Lichao Gong, Feng Jiang, Qi Lv, Songtao Shou, Heng Jin

“…Additionally, a dual-luciferase reporter gene assay was conducted to validate the binding effect of SERPINE1 and GATA2.ResultsTranscriptome Analysis revealed that genes including GATA2 and SERPINE1 were downregulated in HK-2 cells following tocilizumab treatment. …”
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