Therapeutic Target Discovery for Multiple Myeloma: Identifying Druggable Genes via Mendelian Randomization by Shijun Jiang, Fengjuan Fan, Qun Li, Liping Zuo, Aoshuang Xu, Chunyan Sun

“…<b>Methods</b>: To identify potential druggable genes associated with MM, we performed Mendelian randomization (MR) analysis. Causal candidates were further validated using a single-tissue transcriptome-wide association study (TWAS), and colocalization analysis was conducted to assess shared genetic signals between gene expression and disease risk. …”
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Revitalizing the Epigenome of Adult Jaw Periosteal Cells: Enhancing Diversity in iPSC-Derived Mesenchymal Stem Cells (iMSCs) by Felix Umrath, Valerie Wendt, Gilles Gasparoni, Yasser Narknava, Jörn Walter, Bernd Lethaus, Josefin Weber, Victor Carriel, Meltem Avci-Adali, Dorothea Alexander

“…Using an optimized protocol, we generated iMSCs with a remarkable osteogenic potential while exhibiting lower expression levels of the senescence markers p16 and p21 compared to the original JPCs. …”
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γ-Aminobutyric Acid Alleviates Salinity-Induced Impairments in Rice Plants by Improving Photosynthesis and Upregulating Osmoprotectants and Antioxidants by Jiaxin Feng, Tianyang Zhou, Yibiao Gu, Chenchen Shu, Kuanyu Zhu, Weiyang Zhang, Hao Zhang, Lijun Liu, Zhiqin Wang, Junfei Gu, Jianchang Yang

“…These helped maintain photosynthesis under saline conditions while reducing water consumption.…”
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Transcriptional and cytokine signatures of Mycobacterium abscessus complex pulmonary disease during disease progression and treatment. by Ayesa Syenina, Yi Hern Tan, Danny Jian Hang Tng, Sandy Xue Qi Sim, Valerie Shyn Yun Chew, Jia Xin Yee, Eugenia Ziying Ong, Eng Eong Ooi, Jenny Guek Hong Low, Dorothy Hui Lin Ng

“…<h4>Methodology/principal findings</h4>To address the gaps in clinically relevant indicators, we profiled whole blood transcriptome and 45 plasma proteins of MABC-PD patients across different disease and treatment phases. …”
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Exogenous L-Cysteine and Its Transport Through CtaP Play a Role in Biofilm Formation, Swimming Motility, and Swarming Motility of <i>Listeria monocytogenes</i> by Mahide Muge Yilmaz Topcam, Nattanicha Prayoonwiwat, Carolina Bruschi, Kimon Andreas G. Karatzas

“…To evaluate the role of cysteine and CtaP in biofilm formation and motility, transcriptome analysis, comparing WT and Δ<i>ctaP</i> in basal and L-cysteine-supplemented (1.57 and 3.67 mM) DM, was conducted at 37 °C. …”
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Deciphering aging-associated prognosis and heterogeneity in gastric cancer through a machine learning-driven approach by Jiang Li, Chuanlai Yang, Yunxiao Zhang, Xiaoning Hong, Mingye Jiang, Zhongxu Zhu, Jiang Li

“…High AAI scores associated with poor prognosis and indicated potential benefits from adjuvant chemotherapy, while showing resistance to immunotherapy. Single-cell transcriptome analysis revealed that AAI was enriched in monocyte cells within the tumor microenvironment. …”
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A miR-219-5p-bmal1b negative feedback loop contributes to circadian regulation in zebrafish by Lianxin Wu, Meng Zhao, Xifeng Chen, Han Wang

“…Cell transfection and in Vivo assays show that miR219-5p inhibits bmal1b through binding to its 3’UTR. Further, transcriptome analysis of miR-219-5p knockdown zebrafish adult brain reveals possible roles of miR-219-5p in phototransduction and neuroactive ligand-receptor interaction. …”
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Lysionotin promoted apoptosis of hepatocellular carcinoma cells via inducing autophagy by Xiaoxue Wang, Weiwei Zhong, Qin Wang, Peng Song, Xia Lin, Bohan Li, Yancun Yin, Chunyan Yang, Minjing Li

“…Results RNA sequencing analysis, electron microscopy and laser confocal microscopy revealed that lysionotin initiate autophagy in liver cancer cells. …”
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Digital spatial profiling of segmental outflow regions in trabecular meshwork reveals a role for ADAM15. by Jennifer A Faralli, Mark S Filla, Yong-Feng Yang, Ying Ying Sun, Kassidy Johns, Kate E Keller, Donna M Peters, Donna M Peters

“…In this study we used a spatial transcriptomics approach to identify genes specifically associated with either high or low outflow regions in the trabecular meshwork (TM) that could potentially affect aqueous humor outflow in vivo. …”
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A Novel Ferroptosis-Related Gene Prognosis Signature and Identifying Atorvastatin as a Potential Therapeutic Agent for Hepatocellular Carcinoma by Ling Wang, Xiaoqin He, Yang Shen, Jiayu Chen, Yukai Chen, Zhuolin Zhou, Ximing Xu

“…Using various regression and survival analysis techniques, we developed a prognostic model based on four core genes and evaluated its predictive potential. …”
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Fusobacterium nucleatum as an oral bacteria promotes the carcinogenesis of laryngeal cancer via upregulating YWHAZ by Yiyue Ren, Jing Ye, Liuhong Shi, Kehao Le, Yifan Wang

“…Background and purpose F. nucleatum, a gram-negative oral bacteria, is abundant in laryngeal cancer (LC). While specific 14-3-3 proteins act as LC oncogenes, the link between F. nucleatum and 14-3-3 proteins dysregulation remains unknown.Materials and methods Transcriptome data from 520 HNSC patients were obtained from TCGA. …”
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Strigolactones optimise plant water usage by modulating vessel formation by Jiao Zhao, Dongbo Shi, Kiara Kaeufer, Changzheng Song, Dominik Both, Anna Lea Thier, Hui Cao, Linus Lassen, Xiaocai Xu, Yuki Hamamura, Laura Luzzietti, Tom Bennett, Kerstin Kaufmann, Thomas Greb

“…Here, using single-cell transcriptome analysis in Arabidopsis thaliana, we reveal that the strigolactone (SL) signalling pathway negatively regulates vessel element formation, impacting plant water usage. …”
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Enhancement of carrimycin production in Streptomyces spiramyceticus 54-IA by the reporter-guided selection method with traditional mutagenesis by Kemeng Li, Jianlu Dai, Yue Chang, Tianyi Hao, Weiqing He

“…The high-yield mutant F2R-15 was about 13 times higher than that of 54-IA strain, and its titer reached 1010 ± 30 µg/mL. A comparative analysis of the transcriptome profiles between the F2R-15 mutant and 54-IA strains revealed 158 significantly differentially expressed genes. …”
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Hypoxic transcriptional phenotype and cellular ultrastructural changes of tumor-associated macrophages in gliomas by FAN Haizhen, FAN Haizhen, WANG Lixia, CHENG Yue

“…RT-qPCR and transcriptome sequencing were employed to analyze transcriptional changes in TAMs under normoxic and hypoxic conditions. …”
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Role of Maternal Obesity in Offspring Cardiovascular Development and Congenital Heart Defects by Ashleigh McMullan, James B. Zwierzynski, Nina Jain, Laura S. Haneline, Weinian Shou, Kok Lim Kua, Swetansu K. Hota, Matthew D. Durbin

“…Embryos were generated and their developing hearts analyzed. Transcriptome analysis was performed using single‐nucleus and bulk RNA sequencing. …”
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Identification of the metabolic protein ATP5MF as a potential therapeutic target of TNBC by Kaiyan Chen, Yingchun Wu, Linfeng Xu, Changyong Wang, Jinqiu Xue

“…Methods For this research, the scRNA data was obtained from the GEO database, while the transcriptome data was obtained from the TCGA and METABRIC databases. …”
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<i>Polygonatum sibiricum</i> Polysaccharides Alleviate LPS-Induced Liver Injury in Chicks by Suppressing Inflammation and Oxidative Stress Through the PPAR Signaling Pathway by Yang Li, Jian Li, Xiaowang Liu, Zhili Cheng, Nana Gao, Jungang Kang, Xiaodan Wang

“…During this period, the LPS and PSP groups were intraperitoneally injected with 1500 μg/kg LPS on days 14, 16, 18, and 20, while the CON group received normal saline. On day 21, organs were collected for analysis. …”
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Gestational high-sucrose diet mediated vascular hyper-contractility in mesenteric arteries from offspring by Xinying Liu, Meng Liu, Chunxia Wang, Liting Duan, Qinggui Ren, Shuli Jiang, Jing Han, Hongwei Fu, Xiao Sun, Dongmei Man, Xueqin Feng

“…Prenatal HS mediated higher vasoconstriction/vascular sensitivity induced by phenylephrine (PE) and 5-Hydroxytryptamine (5-HT). RNA-Seq analysis revealed that the genes crystallin alpha B (CYRAB) and heat shock protein family E member 1 (HSPE1) were upregulated, while the gene adenomatous polyposis coli downregulated 1 (APCDD1) was downregulated in HS group, confirmed at mRNA and protein expression levels. …”
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Knockout of bcas3 gene causes neurodevelopment defects in zebrafish by Huihui Liu, Nianyi Sun, Zhenxing Liu, Jinze Li, Xianqin Zhang

“…Locomotor behaviors, including performance in the light-dark test, novel tank test, mirror test, shoaling test, and social test, were assessed through video tracing and quantitative analysis of movement parameters. Transcriptome sequencing analysis was used to identify dysregulated pathways associated with development process. …”
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The Spatial‐Temporal Alternative Splicing Profile Reveals the Functional Diversity of FXR1 Isoforms in Myogenesis by Wei Wang, Xinhao Fan, Weiwei Liu, Yuxin Huang, Shuhong Zhao, Yalan Yang, Zhonglin Tang

“…Transcriptome analysis suggests that specifically knocking‐down Fxr1E15+ isoform in myoblasts modulates differentiation by influencing gene expression and splicing of specific targets. …”
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