-
1181
TPS genes expression pattern and terpenoids content in the leaves and flowers of Chimonanthus praecox and C. salicifolius
Published 2025-06-01“…We identified 63 and 48 TPS genes from the genomes of C. praecox and C. salicifolius. Transcriptome analysis showed that TPS-b subfamily genes (related to monoterpene synthesis) showed high expression levels in flowers and leaves, while TPS-a subfamily genes (related to sesquiterpene synthesis) showed strong expression in leaves. …”
Get full text
Article -
1182
Effects of sun shading on anthocyanin components and the expression of genes related to anthocyanin synthesis in Marselan grape
Published 2025-01-01“…During the complete veraison stage and shading during the harvesting stage, the expression levels of PAL, CHS3, F3’H, F3’5’H, UFGT, GST4 and MYB90 genes were upregulated compared to other treatments during the harvesting stage. qRT-PCR analysis revealed that among the 15 screened genes, the expression levels of CHI, F3H, F3’5’H, F3’H, DFR, LDOX, UFGT and OMT showed inconsistent trends with transcriptome sequencing in each treatment, while the changes in other genes were consistent with transcriptome sequencing results. …”
Get full text
Article -
1183
Screening of ferroptosis genes related to the prognosis of cervical cancer and construction of a prognostic model
Published 2025-04-01“…Methods Ferroptosis genes were obtained from FerrDb database, and cervical cancer related data were obtained from The Genome-Wide Association Study Catalog database and The Cancer Genome Atlas database. Transcriptome-Wide Association Study, colocalization analysis and differential expression analysis were conducted to screen out candidate ferroptosis genes; Gene Ontology functional and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were conducted on candidate genes. …”
Get full text
Article -
1184
Genome-wide identification and expression of the HB gene family during overwintering in Korla pear (Pyrus sinkiangensis)
Published 2025-01-01“…Subcellular localization analysis showed that six members (PsHB90, PsHB54, PsHB15, PsHB48, PsHB36 and PsHB12) were localized in the chloroplast, while the others were localized in the nucleus. …”
Get full text
Article -
1185
m6A-lncRNA landscape highlights reduced levels of m6A modification in glioblastoma as compared to low-grade glioma
Published 2025-05-01“…LGG dispersed between C1 and C2 clusters while GB stayed mainly in C1. Clinical feature association analysis between m6A clusters showed the tendency of m6A to be associated with higher malignancy grade (p = 0.053), while significant association was observed with higher Ki-67 proliferation index (p = 0.04), and tumor location (p < 0.01). …”
Get full text
Article -
1186
Genome‑wide identification of the cotton FAR gene family reveals GhFAR3 as a positive regulator of Verticillium dahliae resistance
Published 2025-07-01“…Promoter cis-element analysis and transcriptome profiling revealed that while most FAR genes were downregulated under abiotic stress, many were notably upregulated in response to hormone treatments and Verticillium dahliae infection. …”
Get full text
Article -
1187
Establishment of rat model for aspiration pneumonia and potential mechanisms
Published 2025-06-01“…Techniques included rat weight measurement, tracheal intubation, pulmonary function monitoring, lung tissue sampling with HE staining and scoring, bronchoalveolar lavage fluid (BALF) sampling, protein and inflammatory cytokine analysis via BCA and ELISA, BALF pH determination, Evans Blue dye assessment, blood gas analysis, FITC‐dextran leakage, Western blotting, electron microscopy, survival analysis, and transcriptome sequencing with bioinformatics. …”
Get full text
Article -
1188
Genome-wide exonic small interference RNA-mediated gene silencing regulates sexual reproduction in the homothallic fungus Fusarium graminearum.
Published 2017-02-01“…Various ascomycete fungi possess sex-specific molecular mechanisms, such as repeat-induced point mutations, meiotic silencing by unpaired DNA, and unusual adenosine-to-inosine RNA editing, for genome defense or gene regulation. Using a combined analysis of functional genetics and deep sequencing of small noncoding RNA (sRNA), mRNA, and the degradome, we found that the sex-specifically induced exonic small interference RNA (ex-siRNA)-mediated RNA interference (RNAi) mechanism has an important role in fine-tuning the transcriptome during ascospore formation in the head blight fungus Fusarium graminearum. …”
Get full text
Article -
1189
Evolution of canonical circadian clock genes underlies unique sleep strategies of marine mammals for secondary aquatic adaptation.
Published 2025-03-01“…Remarkably, in vitro assays showed that cetacean-specific mutations increased the nuclear localization of zebrafish clocka, and enhanced the transcriptional activation activity of Clocka and Bmal1a. In vivo, transcriptome analysis showed that the overexpression of the cetacean-specific mutant clocka (clocka-mut) caused the upregulation of the wakefulness-promoting glutamatergic genes and the differential expression of multiple genes associated with sleep regulation. …”
Get full text
Article -
1190
Cuminaldehyde downregulates folate metabolism and membrane proteins to inhibit growth of Penicillium digitatum in citrus fruit
Published 2024-03-01“…These were confirmed by RT‐qPCR analysis. Reduced expression of proteins integral to mETC complexes signaled possible damage to inner mitochondrial membrane. …”
Get full text
Article -
1191
TET3 is a positive regulator of mitochondrial respiration in Neuro2A cells.
Published 2024-01-01“…We report the results of an unbiased transcriptome analysis of the neuroblastoma-derived cell line; Neuro2A, in which Tet3 was silenced. …”
Get full text
Article -
1192
A Fragment Insertion of <i>AgDFR</i> Results in a White Flower Phenotype in <i>Arundina graminifolia</i> (Orchidaceae)
Published 2025-05-01“…Notably, <i>AgDFR</i> expression remained undetectable across all white-flower developmental stages. Sequence analysis demonstrated identical 3030 bp promoter regions of <i>AgDFR</i> between two variants, while white-flower <i>AgDFR</i> coding sequences contained over 107 bp insertion after the 330th nucleotide, causing premature translation termination. …”
Get full text
Article -
1193
Transcriptional profiling of Chlamydia trachomatis and its host in an ex vivo endocervical primary cell culture system using dual RNA sequencing
Published 2025-06-01“…Inclusion-membrane genes peaked at 1hpi, while hemolysin-like and polymorphic membrane protein genes were upregulated at 24hpi. …”
Get full text
Article -
1194
Insights into the Underlying Mechanism of the <i>Piriformospora indica</i>-Enhanced Drought Tolerance in Blueberry
Published 2025-05-01“…To explore the underlying mechanism, comparative root transcriptome analysis of well-watered (WW) and DS-treated CK and PI blueberry plants was conducted. …”
Get full text
Article -
1195
Deubiquitinase OTUD7B stabilizes HNF4α to alleviate pressure overload-induced cardiac hypertrophy by regulating fatty acid oxidation and inhibiting ferroptosis
Published 2025-03-01“…Mechanistically, transcriptomic analysis identified OTUD7B plays a role in the regulation of fatty acid metabolism and pathological cardiac hypertrophy. …”
Get full text
Article -
1196
Assessment of Genetic Diversity by Morphological, Biochemical, and Molecular Markers in <i>Gloriosa superba</i> Ecotypes Collected from Different Agro-Climatic Zones in India
Published 2025-06-01“…Intermediate CV values (10.05% to 18.75%) were observed in eight traits, while three traits (days to flowering, days to 50% flowering, and colchicine content) had low variation (<5%). …”
Get full text
Article -
1197
The Glutamatergic System Regulates Feather Pecking Behaviors in Laying Hens Through the Gut–Brain Axis
Published 2025-04-01“…Correlation analysis indicated that <i>GRIN2A</i> and <i>SLC17A6</i> are positively associated with arginine levels in the duodenum, while <i>Romboutsia</i> in the duodenum is negatively correlated with arginine. …”
Get full text
Article -
1198
AMF30a promotes survival and function of human corneal endothelial cells by regulating TGF-β/ROCK/HIPPO pathway
Published 2025-08-01“…AMF30a modulated the HIPPO signaling pathway by increasing YAP phosphorylation and reducing its nuclear translocation, while downregulating ERK1/2 activation. Transcriptome analysis identified differentially expressed genes (DEGs) associated with AMF30a treatment, highlighting pathways related to GPCR signaling and protein targeting. …”
Get full text
Article -
1199
Identification of telomere maintenance-driven molecular subtypes in hepatocellular carcinoma: implications for prognosis and targeted therapy via KPNA2
Published 2025-04-01“…Despite previous publications highlighting the correlation between individual TM-related genes and HCC, comprehensive exploration and systematic analysis of these genes are still lacking. Methods Through the analysis of transcriptome data, we identified two distinct clusters (termed telomere maintenance-associated clusters, or TCs) that exhibited marked heterogeneity in clinical traits and the tumor microenvironment. …”
Get full text
Article -
1200
Bojungikki-Tang Augments Pembrolizumab Efficacy in Human PBMC-Injected H460 Tumor-Bearing Mice
Published 2024-09-01“…Additionally, RNA sequencing, transcriptome analysis, and quantitative PCR analysis were performed on tumor tissues to investigate the molecular mechanisms underlying the observed effects. …”
Get full text
Article