Overexpression of the ABA Synthesis Gene OsABA2 Enhances Seed Storability in Rice by Yongxiang Liao, Pengfei Jiang, Mingming Zhang, Fangli Jin, Mengyuan Li, Jiazhi Dong, Daiming Guo, Yingxiu Liao, Asif Ali, Saira Saleem, Xiangyang Zhang, Qinggui Jiang, Su Liu, Hongyu Zhang, Xiaoqiong Chen, Kangxi Du, Hao Zhou, Duo Xia, Peizhou Xu, Xianjun Wu

“…The germination rate of OsABA2-OE lines was significantly higher, while the electrical conductivity of the seed leachate was lower compared to the wild type (WT), indicating improved seed vigor. …”
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An Integrated Approach Utilizing Single-Cell and Bulk RNA-Sequencing for the Identification of a Mitophagy-Associated Genes Signature: Implications for Prognostication and Therapeu... by Yuke Zhang, Li Ding, Zhijin Zhang, Liliang Shen, Yadong Guo, Wentao Zhang, Yang Yu, Zhuoran Gu, Ji Liu, Aimaitiaji Kadier, Jiang Geng, Shiyu Mao, Xudong Yao

“…Target genes were narrowed down at the bulk transcriptome level to construct a PARD prognosis prediction nomogram. …”
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A novel module of MaMADS31-MaBZR2 confers negative regulation of banana fruit ripening by Chaojie Wu, Wei Wei, Danling Cai, Zengxiang Lin, Yingying Yang, Jia Si, Wei Shan, Jianye Chen, Wangjin Lu, Jianfei Kuang

“…Fruit ripening, which is modulated by the up- and downregulation of numerous genes, is a sophisticated physiological event determining consumer acceptability. While many positive regulators have been known to regulate fruit ripening, relatively less information is associated with the negative regulators in the process. …”
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Comparative genomics uncovers organellar genome structural divergence in Caryophyllales and reveals widespread non-coding transcription in Bougainvillea glabra organellar by Shuo Zhang, Shengxin Chang, Xinge Lin, Shisong Xu, Qingyun Leng, Haiyan Li, Hernán Ariel López, Junmei Yin, Zhiqiang Wu, Junhai Niu

“…In this study, the organellar genomes and transcriptome of Bougainvillea glabra (Nyctaginaceae) were sequenced and assembled using PacBio sequencing and strand-specific RNA sequencing, respectively. …”
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Comprehensive analysis of the multifaceted role of ITGAV in digestive system cancer progression and immune infiltration by Xinyue Shi, Jingyu Zang, Qi Gu, Mengmeng Zhang, Handi Sun, Lijun Yang, Jiahui Cheng, Ruonan Wang, Han Mao, Aitong Xu, Xin Wang, Yu Xiao, Jialing Cai, Fang Han, Depeng Yang, Yu Li, Huan Nie

“…In vitro, results showed that the knockdown of ITGAV significantly inhibited the migratory movement ability of hepatocellular carcinoma and pancreatic cancer cells, while its overexpression significantly promoted the migration of the above cells. …”
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Temporal changes in the protein cargo of extracellular vesicles and resultant immune reprogramming after severe burn injury in humans and mice by Micah L. Willis, Micah L. Willis, Roland F. Seim, Laura E. Herring, Angie L. Mordant, Thomas S. Webb, Gilbert R. Upchurch, Ashish K. Sharma, Bruce A. Cairns, Philip A. Efron, Philip A. Efron, Shannon M. Wallet, Leon G. Coleman, Leon G. Coleman, Robert Maile, Robert Maile

“…Using unbiased immune transcriptome and bioinformatic causal network analyses, the immunomodulatory effects of these EVs were assessed in human THP-1 macrophages. …”
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Enhanced fibrotic potential of COL1A1hiNR4A1low fibroblasts in ischemic heart revealed by transcriptional dynamics heterogeneity analysis at both bulk and single-cell levels by Cheng Luo, Cheng Luo, Cheng Luo, Baoping Tan, Luoxiang Chu, Liqiang Chen, Xinglong Zhong, Yangyang Jiang, Yuluan Yan, Fanrui Mo, Hong Wang, Fan Yang, Fan Yang

“…Cluster2 exhibited the highest gene activity in terms of fibrosis-related transcriptome dynamics. The characteristic gene expression profile of this FB subset indicated a specific upregulation of COL1A1 and several pro-fibrotic factors, including CCDC102B, GUCY1A3, TEX41, NREP, TCAP, and WISP, while showing a downregulation of NR4A1, an endogenous inhibitor of the TGF-β pathway. …”
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The research on cycloastragenol in the treatment of brain metastases from lung cancer: mechanistic exploration of radiotherapy sensitization and amelioration of brain injury by Yanyan Tao, Jingwen Chang, Jingwen Chang, Xinyi Zhu, Xinyi Zhu, Jingjing Han, Jingjing Han, Xinru Wang, Xinru Wang, Yun Sheng, Yun Sheng, Ziyi Sun, Ziyi Sun, Fang Liu, Fang Liu, Yu Tao, Yu Tao, Hongyan Wu, Chen Yu, Hao Liu, Hao Liu, Fangtian Fan, Fangtian Fan

“…Neuroinflammatory responses in brain tissues were detected via immunofluorescence and qPCR. Transcriptome sequencing and network pharmacology were utilized to identify potential targets and mechanisms, while molecular docking validated interactions between CAG and key targets. …”
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CircFBLN2 regulates duck myoblast proliferation and differentiation through miR-22-5p and MEF2C interaction by Shuibing Liu, Jintao Wu, Hongxia Jiang, Ya'nan Zhou, Xuwen Huang, Yuxiang Wang, Zhanbin Xie, Zurong Liao, Zhenxvan Ding, Jing Liu, Xiaolong Hu, Huirong Mao, Sanfeng Liu, Biao Chen

“…Our findings indicated that circFBLN2 inhibits DPM proliferation while promoting their differentiation. Conversely, when miR-22-5p was silenced and overexpressed, it exhibited opposing effects by promoting the proliferation of DPMs and inhibiting their differentiation. …”
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Multiomics profiling reveals the benefits of gamma-delta (γδ) T lymphocytes for improving the tumor microenvironment, immunotherapy efficacy and prognosis in cervical cancer by Xiaofeng Wang, Chen Li, Jie Chen, Zhiqiang Wu, Zhen Zhang, Junyi Li, Lu Yu, Yong Guan, Yuhan Zhang, Yuanjie Cao, Yancheng Liu, Hui Bai, Shaochuan Liu, Miaomiao Gao, Chenglu Lu, Zhen Tao, Zhiyong Yuan

“…Recent studies have indicated that γδ T cells play contrasting roles in tumor microenvironments—promoting tumor progression in some cancers (eg, gallbladder and leukemia) while suppressing it in others (eg, lung and gastric). γδ T cells are mainly enriched in peripheral mucosal tissues. …”
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Identification of heat shock transcription factor gene families in sunflower and their expression profiles under abiotic stress(向日葵热激转录因子基因家族鉴定及其在非生物胁迫下的表达分析)... by 宋慧芳(SONG Huifang), 薛宏宇(XUE Hongyu), 王茜(WANG Xi), 卢俊洁(LU Junjie), 李晨雨(LI Chenyu), 刘阿克(LIU Ake)

“…Furthermore, the expression patterns of HaHSF genes were observed in various tissues and under hormone or abiotic stress conditions by analyzing transcriptome analysis. HaHSF12, HaHSF22, HaHSF27, and HaHSF33 showed responsiveness to exogenous plant hormones such as abscisic acid, salicylic acid; while HaHSF17, HaHSF18, and HaHSF33 might be involved in conferring abiotic stress resistance on sunflower seedlings. …”
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LncRNA PELATON regulates Th2 cell differentiation by miR-10b-5p/GATA3 axis in allergic rhinitis by Zhen Liu, MD, Yumei Li, PhD, Xiangkun Zhao, MM, Qi Sun, MD, Yu Zhang, MD, Yaqi Wang, MM, Han Fang, MD, Yujuan Yang, MD, Yakui Mou, MD, Xicheng Song, MD

“…Modulation of miR-10b-5p and GATA3 expression by PELATON was studied via transcriptome sequencing, dual-luciferase reporter assays, and in vitro experiments. …”
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CircSLC22A3 inhibits the invasion and metastasis of ESCC via the miR-19b-3p/TRAK2 axis and by reducing the stability of m6A-modified ACSBG1 mRNA by Yingjie Pan, Hang Yang, Jiayi Zhang, Ruolan Zhang, Yun Liu, Jun Bie, Qiaoling Chen, Yan Qiao, Kang Liu, Guiqin Song

“…Methods CircSLC22A3 expression in ESCC tissues and cells was analyzed using transcriptome sequencing and RT-qPCR. Its circular structure was validated through Sanger sequencing, agarose gel electrophoresis, RNase R digestion, and random priming assays. …”
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IL1RAP is an immunotherapeutic target for normal karyotype triple-mutated acute myeloid leukemia by Arnaud Métois, Marie-Eve Bordeleau, Louis Theret, Azadeh Hajmirza, Ossama Moujaber, Jean-François Spinella, Jalila Chagraoui, Nadine Mayotte, Isabel Boivin, Éric Audemard, Léo Aubert, Véronique Lisi, Banafsheh Khakipoor, Azer Farah, Éric Bonneil, Alma Robert, Julie Lippens, Anna Moraitis, François Béliveau, Albert Feghaly, Geneviève Boucher, Richard Marcotte, Patrick Gendron, Pierre Thibault, Sébastien Lemieux, Guillaume Richard-Carpentier, Vincent-Philippe Lavallée, Josée Hébert, Philippe P. Roux, Guy Sauvageau

“…We demonstrate that IL1RAP is expressed at the surface of primitive AML cells reminiscent of leukemic stem cells in NKt-AML primary human AML specimens, while showing relatively low expression levels in normal bone marrow HSCs. …”
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Lycium barbarum glycopeptide ameliorates aging phenotypes and enhances cardiac metabolism by activating the PINK1/Parkin-mediated mitophagy pathway in D-galactose-induced mice by Tianchan Peng, Jian Xiang, Yun Tian, Xiaogen Tang, Lina Wang, Lijuan Gao, Oscar Junhong Luo, Li’an Huang, Guobing Chen

“…Methods: Mice were subcutaneously injected with D-galactose (500 mg/kg/day) for 12 weeks to induce aging, while LbGp was orally administered (100 mg/kg/day) throughout the study. …”
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Translating Muscle RNAseq Into the Clinic for the Diagnosis of Muscle Diseases by Alba Segarra‐Casas, Cristina Domínguez‐González, Daniel Natera‐de Benito, Solange Kapetanovic, Aurelio Hernández‐Laín, Berta Estévez‐Arias, Laura Llansó, Carlos Ortez, Cristina Jou, Itxaso Martí‐Carrera, Arístides López‐Márquez, Maria José Rodríguez, Laura González‐Mera, Velina Nedkova, Roberto Fernández‐Torrón, Benjamín Rodríguez‐Santiago, Cecília Jimenez‐Mallebrera, Raul Juntas‐Morales, Adolfo López‐de Munain, Jordi Surrallés, Andrés Nascimento, Eduard Gallardo, Montse Olivé, Pia Gallano, Lidia González‐Quereda

“…Here, we implemented transcriptome analysis of muscle biopsies as a complementary diagnostic tool for patients with muscle disease but no definitive genetic diagnosis after exome sequencing. …”
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Evaluating metagenomics and targeted approaches for diagnosis and surveillance of viruses by Sarah Buddle, Leysa Forrest, Naomi Akinsuyi, Luz Marina Martin Bernal, Tony Brooks, Cristina Venturini, Charles Miller, Julianne R. Brown, Nathaniel Storey, Laura Atkinson, Timothy Best, Sunando Roy, Sian Goldsworthy, Sergi Castellano, Peter Simmonds, Heli Harvala, Tanya Golubchik, Rachel Williams, Judith Breuer, Sofia Morfopoulou, Oscar Enrique Torres Montaguth

“…Protocols were designed to retain the host transcriptome, since this could help confirm the absence of infectious agents. …”
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Deciphering human endogenous retrovirus expression in colorectal cancers: exploratory analysis regarding prognostic value in liver metastasesResearch in context by Julien Viot, Romain Loyon, Nawfel Adib, Pierre Laurent-Puig, Aurélien de Reyniès, Fabrice André, Franck Monnien, Thierry André, Magali Svrcek, Anthony Turpin, Zohair Selmani, Laurent Arnould, Laura Guyard, Nicolas Gilbert, Anthony Boureux, Olivier Adotevi, Angélique Vienot, Syrine Abdeljaoued, Dewi Vernerey, Christophe Borg, Daniel Gautheret

“…Summary: Background: Human Endogenous RetroVirus (HERV) expression in tumours reflects epigenetic dysregulation of cancer and an oncogenic factor through promoter/enhancer action on genes. While more than 50% of colorectal cancers develop liver metastases, HERV has not been studied in this context. …”
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GmBSK1-GmGSK1-GmBES1.5 regulatory module controls heat tolerance in soybean by Ze-Hao Hou, Yuan Gao, Jia-Cheng Zheng, Meng-Jie Zhao, Ying Liu, Xiao-Yu Cui, Zhi-Yong Li, Ji-Tong Wei, Tai-Fei Yu, Lei Zheng, Yuan-Chen Jiao, Shu-Hui Yang, Jia-Min Hao, Jun Chen, Yong-Bin Zhou, Ming Chen, Lijuan Qiu, You-Zhi Ma, Zhao-Shi Xu

“…Furthermore, we identified an interaction between GmGSK1 and GmBES1.5, while GmGSK1 inhibits the transcriptional activity of GmBES1.5. …”
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The involvement of Argonaute molecule PIWI in the antiviral immunity of oyster Crassostrea gigas by Yuhao Jin, Xue Qiao, Yuqing Zeng, Yiqing Wang, Lilin Hou, Xinyu Zhao, Sicong Wang, Lingling Wang, Linsheng Song

“…Both rCgPIWI and rCgPIWI-PAZ demonstrated significant binding activity to ssRNA in vitro, while no binding activity to dsRNA. Additionally, rCgPIWI and rCgPIWI-PIWI exhibited substantial RNase activity, efficiently cleaving ssRNA in vitro. …”
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