Effect of polystyrene micro/nanoplastics on mesenchymal phenotypic transformation in testicular Sertoli cells by CAI Jing, WEN Yixian, ZHANG Huilian

“…Cell viability and proliferative capacity were evaluated using CCK-8 assay and EdU incorporation, while cell migration was assessed using transwell and cell scratch assays. …”
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snRNA-seq reveals subcutaneous white adipose tissue remodeling upon return to thermoneutrality after cold stimulation by Yusha Yang, Yusha Yang, Guanyu Zhang, Ting Yi, Ting Yi, Shuran Yang, Shuran Yang, Shuai Wu, Yongqiang Zhang, Li Zhang, Xi Li, Xiuxuan Wu, Xiuxuan Wu, Jun Li, Danfeng Yang

“…Subsequently, we employed single-nucleus RNA sequencing (snRNA-seq) to comprehensively characterize iWAT cellular dynamics during cold stimulation and the subsequent two-week period after the return to thermoneutrality.ResultsOur findings revealed that while iWAT phenotypically reverts to a white state after 2 weeks of rewarming, as evidenced by structural, functional, and bulk transcriptomic characteristics, significant cold-induced molecular and cellular signatures persist. …”
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Molecular characterization and expression profiles of cytochrome P450 reductase gene in Sogatella furcifera (Hemiptera: Delphacidae) by YU Hang, LIU Su, ZHU Qingzi, ZHOU Wenwu, LIANG Qingmei, SHI Xiaoxiao, ZHU Zijie, ZHU Zengrong

“…The expression of SfCPR was activated soon (6 h) after treatment with buprofezin and imidacloprid, while the response of SfCPR expression to deltamethrin was relatively slow (12 h).In conclusion, this work is the first report about the cDNA sequence information, secondary structure and transcription profiles of CPR gene in the S. furcifera. …”
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Cloning and Bioinformatics Analysis of Vitellogenin Genes and Its Receptor Genes in Thalassodes immissaria by Guolin ZHU, Qiong YAO, Wenjing LI, Shu XU, Kai LIU, Shuanggang DUAN

“…【Objective】Vitellogenin (Vg) and its receptor (VgR) play key roles in insect reproduction by mediating nutrient transport during oocyte vitellogenesis.This study aimed to clone Vg and VgR from the Thalassodes immissaria, a notorious defoliator in lichee, and analyzed their bioinformatics characteristics and expression patterns across developmental stages to infer their roles in reproductive regulation.【Method】Using transcriptome data of T.immissaria, the full-length cDNA sequences of TiVg and TiVgR were cloned via PCR.Bioinformatics tools, such as ORF Finder, SignalP 5.0, ProtParam, SMART, TMHMM 2.0, and MEGA v5.05, were used for sequence analysis.Expression profiles of TiVg and TiVgR across different developmental stages and different tissues were quantified using transcriptomic TPM values and qRT-PCR.…”
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OTUD1 regulates cytokine expression and related pathways in goose fatty liver by promoting deubiquitination of its target proteins by Xiaoyi Zhou, Ya Xing, Yuqing Wang, Mengqing Lv, Pei Zhang, Suyan Zhu, Jing Ge, Long Liu, Minmeng Zhao, Haizhou Gong, Daoqing Gong, Tuoyu Geng

“…In goose primary hepatocytes, palmitic acid and oleic acid both increased the protein levels of OTUD1, while glucose and insulin inhibited the expression of the protein. …”
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TLR3-overexpressing umbilical cord mesenchymal stromal cells suppress immune responses to attenuate high-risk corneal transplantation rejection by Yaqi Cheng, Huaxin Chen, Simin Gu, Weihua Li, Huan Yu, Jianqiang Zhang, Huini Zhang, Jiayi Lin, Haocheng Zhu, Youyu Liu, Wenqiong Li, Ting Fu, Haoyu Zeng, Tao Wang, Shiqi Ling

“…Methods New Zealand rabbits that underwent high-risk corneal transplantation were treated with UC-MSC-coated contact lenses (MSCohi-O), while blank lenses and untreated grafts served as controls. …”
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In Vitro Investigation of Statin Effects on Genes Associated with Severe COVID-19 in Cancerous and Non-Cancerous Cells by Adriana Kapustová, Patrik Macášek, Bibiána Baďurová, Jana Melegová, Silvie Rimpelová, Jan Kubovčiak, Jana Šáchová, Miluše Hradilová, Michal Kolář, Libor Vítek, Tomáš Ruml, Helena Gbelcová

“…<b>Conclusions:</b> Statins act on multiple pathways simultaneously, some of which support COVID-19 development, while others suppress it.…”
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Structural and regulatory determinants of flagellar motility in Rhodobacterales—the archetypal flagellum of Phaeobacter inhibens DSM 17395 by Jürgen Tomasch, Pascal Bartling, John Vollmers, Lars Wöhlbrand, Michael Jarek, Manfred Rohde, Henner Brinkmann, Heike M. Freese, Ralf Rabus, Jörn Petersen

“…Our phylogenetically broad taxon sampling of more than 300 genomes revealed that the most common FGC, the fla1-type, was probably already present in the common ancestor of Rhodobacterales and was strictly vertically inherited, while the other two FGC types, fla2 and fla3, were spread via horizontal operon transfers. …”
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<i>AtAUEs</i>, a Small Family of ABA Up-Regulated EAR Motif-Containing Proteins Regulate ABA Responses in Arabidopsis by Wei Wang, Xutong Wang, Xiaoyu Liu, Yating Wang, Yingying Li, Saddam Hussain, Xiaoxiao Jing, Siyu Chen, Shucai Wang

“…We found that the single and double mutants displayed wild-type responses to ABA treatment, while the <i>ataue123</i> triple mutants showed increased sensitivity in seed germination and cotyledon greening assays but decreased sensitivity to ABA treatment in root elongation assays. …”
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Epigenome profiling reveals distinctive regulatory features and cis-regulatory elements in pepper by Hongji Yang, Guorong Yu, Zhidong Lv, Tonghui Li, Xi Wang, Ying Fu, Zhangsheng Zhu, Guangjun Guo, Hang He, Ming Wang, Guochen Qin, Feng Liu, Zhenhui Zhong, Yan Xue

“…Abstract Background Pepper (Capsicum annuum) is one of the earliest and most widely cultivated vegetable crops worldwide. While the large and complex genome of pepper severely hampered the understanding of its functional genome, it also indicates a rich yet unexplored reservoir of regulatory elements (REs). …”
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MARCKSL1 interacted with F‐actin to promote esophageal squamous cell carcinoma mobility by modulating the formation of invadopodia by Yue Zhao, Xiufeng Xie, Lusong Tian, Fang Liu, Yulin Sun, Haizhen Lu, Xiaohang Zhao, Yousheng Mao

“…Transcriptomic analysis showed that MARCKSL1 mediated the mobility and migration of ESCC cells. …”
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α-Mangostin prevents diabetic cardiomyopathy by inhibiting oxidative damage and lipotoxicity through the AKT–FOXO1–CD36 pathway by Xue Bai, Ziqian Zhang, Miao Zhang, Jiaojiao Xu, Keting Dong, Qian Du, Lei Chen, Ping Ma, Jianhong Yang

“…Molecular mechanisms were investigated through transcriptome analysis, quantitative PCR (RT-qPCR), and Western blotting. …”
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Interplay between oncolytic measles virus, macrophages and cancer cells induces a proinflammatory tumor microenvironment by Camille Chatelain, Laurine Berland, Marion Grard, Nicolas Jouand, Judith Fresquet, Joëlle Nader, Ugo Hirigoyen, Tacien Petithomme, Chantal Combredet, Elvire Pons-Tostivint, Delphine Fradin, Lucas Treps, Christophe Blanquart, Nicolas Boisgerault, Frédéric Tangy, Jean-François Fonteneau

“…These macrophages inhibit GFP expression in tumor cells harboring a defect in IFN-I production and a functional signaling downstream of the IFN-I receptor, while having minimal effects on GFP expression in tumor cells with defect of responsiveness to IFN-I. …”
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Multiomic analysis of monocyte-derived alveolar macrophages in idiopathic pulmonary fibrosis by Miaomiao Zhang, Jinghao Zhang, Haisheng Hu, Yuan Zhou, ZhiWei Lin, Hui Jing, Baoqing Sun

“…Abstract Background Monocyte-derived alveolar macrophages (Mo_AMs) are increasingly recognised as potential pathogenic factors for idiopathic pulmonary fibrosis (IPF). While scRNAseq analysis has proven valuable in the transcriptome profiling of Mo_AMs, the integration analysis of multi-omics may provide additional dimensions of understanding of these cellular populations. …”
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E2F1-induced autocrine IL-6 inflammatory loop mediates cancer-immune crosstalk that predicts T cell phenotype switching and therapeutic responsiveness by Alf Spitschak, Prabir Dhar, Krishna P. Singh, Rosaely Casalegno Garduño, Shailendra K. Gupta, Shailendra K. Gupta, Julio Vera, Luca Musella, Nico Murr, Anja Stoll, Brigitte M. Pützer, Brigitte M. Pützer

“…Clinical data from TCGA demonstrated that elevated E2F1, STAT3, and IL-6 correlate with infiltration of Th2, while simultaneously blocking Th1 in primary and metastatic melanomas. …”
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Highly efficient generation of mature megakaryocytes and functional platelets from human embryonic stem cells by Chuxin Chen, Ning Wang, Xueyan Zhang, Yingjie Fu, Zhiyong Zhong, Haibin Wu, Yaming Wei, Yuyou Duan

“…Furthermore, 95% of platelets showed CD42b+CD62p+ phenotype upon the stimulation with ADP and TRAP-6, while 50% of platelets exhibited the ability to bind PAC-1, indicating that hESC-derived platelets possessed the in vitro functionality. …”
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Single-nucleus RNA sequencing reveals the specific molecular signatures of myeloid cells responding to brain injury after microglial replacement by Xiao-Yi Xiong, Xiao-Yi Xiong, Xiao-Yi Xiong, Haicheng Yuan, Ying Mu, Yi He, Fang Xie, Xiao-Shuang Feng, Jia-Xin Xie, Xin-Ru Pan, Yu-Fei Wang, Jian Gong, Xiaoming Zheng, Peng-Fei Wang

“…Then, a single-nucleus transcriptomic analysis of acutely injured brains in aged mice with microglial replacement was performed.ResultsWe observed similarities but strong divergence in the composition and molecular change features of myeloid cells between the replacement (Rep) and control (Con) groups, indicating retention of the core transcriptome and development of differential genes in myeloid cells after microglial replacement in response to brain injury. …”
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Profiling of lincRNAs and differential regulatory mechanisms in response to nanoplastic toxicity at environmentally relevant concentrations in Caenorhabditis elegans by Xiaoxiao Zhang, Chenxi Zhou, Zifan Wu, Xinyi Jiang, Qinlin Wu, Yuhan An, Zihan Yu, Yunfei Liu, Long Miao, Xing Liu, Man Qu, He Chen

“…Compared to wild-type nematodes, linc-7, linc-9, linc-13, linc-21, linc-24, linc-49, linc-61, and linc-169 KO nematodes exhibited increased sensitivity to the toxicity of PS-NPs, while linc-11 and linc-50 nematodes showed resistance. …”
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Enhancing infectious intestinal disease diagnosis through metagenomic and metatranscriptomic sequencing of 1000 human diarrhoeal samples by Edward Cunningham-Oakes, Blanca M. Perez-Sepulveda, Yan Li, Jay C. D. Hinton, Charlotte A. Nelson, K. Marie McIntyre, Maya Wardeh, Sam Haldenby, Richard Gregory, Miren Iturriza-Gómara, Christiane Hertz-Fowler, Sarah J. O’Brien, Nigel A. Cunliffe, Alistair C. Darby, on behalf of the INTEGRATE consortium

“…Compared with metagenomics, metatranscriptomics had increased sensitivity of detection for four pathogens, while metagenomics was more effective for detecting five pathogens. …”
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Insights into the role of MSLN-positive circulating tumor cell as an auxiliary diagnostic biomarker in epithelial ovarian cancer by Hang Xu, Min Wang, Shuting Wu, Qinke Li, Jinlong Wang, Siying Zhang, Qiongming Liu, Mengting Wang, Ruifang Li, Zhiyuan Hu, Zhiyuan Hu, Yi Liu, Zhu Yang

“…This study aimed to identify and validate novel auxiliary diagnostic markers for EOC.MethodsThrough integrated analysis of transcriptome sequencing data and single-cell RNA sequencing from public databases, we identified mesothelin (MSLN) as an EOC-specific target. …”
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