The first step for understanding the molecular mechanism of the antifibrotic effect of inhaling 25(OH)-vitamin D3 and 1,25(OH)2-vitamin D3 in the murine model of hypersensitivity p... by Marta Kinga Lemieszek, Michał Chojnacki, Iwona Paśnik, Alicja Wilczyńska, Wiktoria Gawryś, Jakub Anisiewicz, Ilona Leśniowska, Michał Kiełbus

“…The study was conducted in VD3-deficient mice, while VD3-sufficient mice were used as the main control. …”
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Transcription regulation of African swine fever virus: dual role of M1249L by Dongming Zhao, Nan Wang, Xiaoying Feng, Zhenjiang Zhang, Kongen Xu, Tao Zheng, Yunge Yang, Xuemei Li, Xianjin Ou, Rui Zhao, Zihe Rao, Zhigao Bu, Yutao Chen, Xiangxi Wang

“…Through analyses of dynamic transcriptome, vRNAP-associated components and cell-based assay, the critical role of M1249L in viral transcription regulation has been highlighted. …”
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E2F1/CDK5/DRP1 axis mediates microglial mitochondrial division and autophagy in the pathogenesis of cerebral ischemia‐reperfusion injury by Ya‐Jing Yuan, Tingting Chen, Yan‐Ling Yang, Hao‐Nan Han, Li‐Ming Xu

“…Conclusions Activation of E2F1‐mediated CDK5 drives mitochondrial division while inhibiting mitophagy in microglia, triggering inflammation, neuronal apoptosis, and exacerbating CIRI damage. …”
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Platelet-derived extracellular vesicles induced through different activation pathways drive melanoma progression by functional and transcriptional changes by Zeynep Tavukcuoglu, Umar Butt, Alessandra V. S. Faria, Johannes Oesterreicher, Wolfgang Holnthoner, Saara Laitinen, Mari Palviainen, Pia R-M Siljander

“…Conclusions Our study revealed both shared and distinct, cancer-promoting functions of PEVs, which contributed to the transcriptome and metastatic capabilities of the melanoma spheroids. …”
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A novel gene signature for predicting outcome in colorectal cancer patients based on tumor cell-endothelial cell interaction via single-cell sequencing and machine learning by Lina Pang, Qingxia Sun, Wenyue Wang, Mingjie Song, Ying Wu, Xin Shi, Xiaonan Shi

“…Specifically, Malignant Cluster01 subpopulations primarily served as signal senders, while Tip-like endothelial cells acted as receivers in PARs signaling. …”
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Unraveling the Multilayered Regulatory Networks of miRNAs and PhasiRNAs in <i>Ginkgo biloba</i> by Qixuan Wei, Ang Xu, Anqi Zhao, Lisha Shi, Qi Wang, Xiaoming Yang, Meiling Ming, Liangjiao Xue, Fuliang Cao, Fangfang Fu

“…We conducted an in-depth analysis of deep sequencing data from the gymnosperm <i>Ginkgo biloba</i>, encompassing sRNA, transcriptome, and degradome libraries. Our analysis identified a total of 746 miRNAs and 654 phasiRNA precursor (PHAS) loci, with 526 (80%) of the PHAS loci predicted to be triggered by 515 miRNAs (69%). …”
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Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice by Anna Schmidt, Jana Fuchs, Mark Dedden, Katharina Kocher, Christine Schülein, Julian Hübner, Ana Vieira Antão, Pascal Irrgang, Friederike Oltmanns, Vera Viherlehto, Natascha Leicht, Ralf Joachim Rieker, Carol Geppert, Uwe Appelt, Sebastian Zundler, Kilian Schober, Dennis Lapuente, Matthias Tenbusch

“…Here we compare TRM populations induced by intranasal adenoviral vector vaccines encoding hemagglutinin and nucleoprotein (NP) with those induced by an H1N1 infection in BALB/c mice. While vaccine-induced TRM express high levels of CD103 and persist longer in the lung parenchyma, short-lived, H1N1-induced TRM have a transcriptome associated with higher cytotoxic potential and distinct transcriptional profile as shown by single-cell RNA sequencing. …”
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Functional analysis of NtPDX2 in Nicotiana tabacum L. associated with stem development by Li Xu, Yuxin Cui, Jiaxin Xing, Qili Mi, Zhixing Wang, Xujing Wang, Wanli Zeng, Haiying Xiang, Jiarui Jiang, Lele Deng, Kunmiao Wang, Jiangtao Yang, Qian Gao

“…By conducting both constitutive overexpression and homozygous knockout studies of the NtPDX2, we observed a significant increase in vitamin B6 content in the stem tissues of overexpressing plants (up to 150%), while knockout plants showed a decrease to 60%. This led to changes in agronomic traits such as plant height and stem thickness in tobacco plants. …”
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Modulation of RNA processing genes during sleep-dependent memory by Yongjun Li, Nitin S Chouhan, Shirley L Zhang, Rebecca S Moore, Sara B Noya, Joy Shon, Zhifeng Yue, Amita Sehgal

“…To better understand the mechanisms underlying sleep and memory consolidation initiation, we analyzed the transcriptome of ap α′/β′ neurons 1 hr after appetitive memory conditioning. …”
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The Mechanism of Aniline Blue Degradation by Short-Chain Dehydrogenase (<i>SDRz</i>) in <i>Comamonas testosteroni</i> by Chuanzhi Zhang, Yong Huang, Jiaxin He, Lei He, Jinyuan Zhang, Lijing Yu, Elshan Musazade, Edmund Maser, Guangming Xiong, Miao Xu, Liquan Guo

“…The knockout strain CT-ΔSDRz exhibited a 1.27-fold reduction in the degradation efficiency, compared to CT1 strain after 12 h; while the expression strain E-SDRz showed a 1.24-fold increase compared to <i>Escherichia coli</i> DH5α after 12 h. …”
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Adventitial fibroblasts direct smooth muscle cell-state transition in pulmonary vascular disease by Slaven Crnkovic, Helene Thekkekara Puthenparampil, Shirin Mulch, Valentina Biasin, Nemanja Radic, Jochen Wilhelm, Marek Bartkuhn, Ehsan Bonyadi Rad, Alicja Wawrzen, Ingrid Matzer, Ankita Mitra, Ryan D Leib, Bence Miklos Nagy, Anita Sahu-Osen, Francesco Valzano, Natalie Bordag, Matthias Evermann, Konrad Hoetzenecker, Andrea Olschewski, Senka Ljubojevic-Holzer, Malgorzata Wygrecka, Kurt Stenmark, Leigh M Marsh, Vinicio de Jesus Perez, Grazyna Kwapiszewska

“…IPAH-PASMC exhibit increased glycosaminoglycan production and downregulation of contractile machinery, while IPAH-PAAF display a hyperproliferative phenotype. …”
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Identification of osteoarthritis-associated chondrocyte subpopulations and key gene-regulating drugs based on multi-omics analysis by Ting Hao, Zhiwei Pei, Sile Hu, Zhenqun Zhao, Wanxiong He, Jing Wang, Liuchang Jiang, jirigala Ariben, Lina Wu, Xiaolong Yang, Leipeng Wang, Yonggang Wu, Xiaofeng Chen, Qiang Li, Haobo Yang, Siqin Li, Xing Wang, Mingqi Sun, Baoxin Zhang

“…To begin, single-cell and transcriptome datasets were obtained from the Gene Expression Omnibus (GEO) database. …”
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Assessing the effects of imidacloprid exposure on the microbiota of Binodoxys communis (Hymenoptera: Braconidae) and gene expression by Qiqing Yu, Weijiao Liu, Likang Zhao, Xiangzhen Zhu, Punniyakotti Elumalai, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Liang Jin, Junyu Luo, Jinjie Cui, Xueke Gao

“…Following treatment with imidacloprid, transcriptomic analysis identified 1263 significantly differentially expressed genes. …”
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Neural correlates differ between crystallized and fluid intelligence in adolescents by Bowen Qiu, Rui Qian, Baorong Gu, Zichao Li, Zhifan Chen, Xinyi Xu, Huaijin Gao, Yiwei Chen, Ruoke Zhao, Ruike Chen, Yuqi Zhang, Lingxuan Zhang, Zhiyong Zhao, Mingyang Li, Dan Wu

“…We further investigated the molecular mechanisms underlying fluid and crystallized intelligence by correlating the characteristic MRI markers with spatial transcriptome profiles and PET imaging. Results showed that fluid intelligence had significant associations with serotonin and glutamate system, while crystallized intelligence was related to serotonin, dopamine and acetylcholine system. …”
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Morphological and Physiological Responses of Tomatoes to Weak Light under Artificial Climate Chamber Conditions by Weijie XU, Yingxue HU, Chao GONG, Baojuan SUN, Zhou HENG, Tao LI

“…Under weak light stress, the activities of SOD, CAT, and POD in 'GC303' leaves were significantly reduced compared with 'LA2838', while MDA showed no significant difference, indicating a stronger peroxide scavenging ability. …”
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Genome-Wide Identification of the <i>DOG1</i> Gene Family in Pepper (<i>Capsicum annuum</i>) and Its Expression Profiles During Seed Germination by Zhichao Zhao, Jingbo Sun, Feng Zhang, Chunjuan Dong

“…Moreover, upon abscisic acid treatment, six <i>CaDOG1</i> genes exhibited upregulation, while in response to ethylene, four <i>CaDOG1</i> genes exhibited downregulation. …”
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Multi-omics investigation reveals unique markers in Klebsiella pneumoniae compared to closely related species by Lena-Sophie Swiatek, Kristin Surmann, Elias Eger, Justus U. Müller, Manuela Gesell Salazar, Stefan E. Heiden, Guido Werner, Nils-Olaf Hübner, Jürgen A. Bohnert, Karsten Becker, Uwe Völker, Michael Schwabe, Katharina Schaufler, Katharina Schaufler

“…Transcriptome and proteome analyses in SHU revealed different regulatory patterns between KP and KV strains, with metabolic responses playing a pivotal role. …”
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Immunoregulatory programs in anti‐N‐methyl‐D‐aspartate receptor encephalitis identified by single‐cell multi‐omics analysis by Xinhui Li, Yicong Xu, Weixing Zhang, Zihao Chen, Dongjie Peng, Wenxu Ren, Zhongjie Tang, Huilu Li, Jin Xu, Yaqing Shu

“…Abstract Background Anti‐N‐methyl‐D‐aspartate receptor encephalitis (anti‐NMDARE) is a prevalent type of autoimmune encephalitis caused by antibodies targeting the NMDAR's GluN1 subunit. While significant progress has been made in elucidating the pathophysiology of autoimmune diseases, the immunological mechanisms underlying anti‐NMDARE remain elusive. …”
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IGF2BP3 Modulates mRNA Splicing and Stability to Promote Trophoblast Progression via Interaction with PDE3A and Suppression by miR-196a-5p in Preeclampsia by Chunyan Li, Pingpo Ming, Cuifang Fan, Jiao Chen, Jing Yang

“…IGF2BP3 overexpression markedly enhanced HTR-8/SVneo trophoblast proliferation, migration, and invasion while suppressing ROS activation. RNA-seq, RIP-seq, and RNA stability assays revealed that IGF2BP3 directly interacts with and enhances the stability of PDE3A mRNA. …”
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Local Inflammation Precedes Diaphragm Wasting and Fibrotic Remodelling in a Mouse Model of Pancreatic Cancer by Daria Neyroud, Andrew C. D'Lugos, Enrique J. Trevino, Chandler S. Callaway, Jacqueline Lamm, Orlando Laitano, Brittney Poole, Michael R. Deyhle, Justina Brantley, Lam Le, Andrew R. Judge, Sarah M. Judge

“…These biological processes were supported by changes in the diaphragm transcriptome. Ingenuity pathway analysis predicted factors involved in inflammatory responses to tissue injury, including TGF‐β1, angiotensin and PDGF BB, as top upstream regulators activated in diaphragms prior to and throughout cachexia progression, while PGC‐1α and the insulin receptor were among the top upstream regulators predicted to be suppressed. …”
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