Exploring the response of yellow lupine (Lupinus luteus L.) root to drought mediated by pathways related to phytohormones, lipid, and redox homeostasis by Sebastian Burchardt, Małgorzata Czernicka, Agata Kućko, Wojciech Pokora, Małgorzata Kapusta, Krzysztof Domagalski, Katarzyna Jasieniecka-Gazarkiewicz, Jacek Karwaszewski, Emilia Wilmowicz

“…Oxidative stress elevates the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT), while the conversion of FFAs into TAGs provides protection against ROS. …”
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Abnormal eye movement, brain regional homogeneity in schizophrenia and clinical high-risk individuals and their associated gene expression profiles by Zhaobin Chen, Yangpan Ou, Yudan Ding, Ying Wang, Huabing Li, Feng Liu, Ping Li, Dongsheng Lv, Yong Liu, Bing Lang, Jingping Zhao, Wenbin Guo

“…FSZ exhibited a wide range of eye movement abnormalities across multiple tasks, while certain abnormalities were already present in CHR. …”
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Zinc sulfate improves insulin resistance, oxidative stress and apoptosis in liver tissues of PCOS rats through the NF-κB pathway by Yi-fan Kang, Jia-yi Zhao, Jian-rong Liu

“…Histological examination of ovarian and liver tissues was performed using hematoxylin and eosin (HE) staining, while hepatic lipid accumulation was evaluated by Oil Red O staining. …”
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Wip1 inhibitor CCT007093 alleviates immune exhaustion of lymphocytes via p65 NF-κB and YY1 in chronic hepatitis B virus infection in mice by Yu-Syuan You, Wan-Ting Chang, Chia-Lang Hsu, Hui-Ying Wang, Yan-Fong Lu, Yan-Fong Lu, InKyeom Kim, InKyeom Kim, InKyeom Kim, Shiang-Jong Tzeng

“…Our detailed analyses, spanning promoter and transcriptome evaluations, uncovered p65 NF-κB as the primary activator of T cells and B cells, while Ying Yang 1 (YY1) emerged as the key regulator, orchestrating the down-regulation of PD-1 and FcγRIIB gene transcription in response to CCT007093.DiscussionOur study highlights the prowess of chemical checkpoint inhibitors, exemplified by CCT007093, in alleviating immune exhaustion in HBV-infected mice, particularly by enhancing adaptive immunity.…”
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Whole blood RNA-seq analysis reveals the immunomodulatory effects of a supplemental multi-strain direct-fed microbial in the diet of newly weaned beef steers1 by Adekunle Ajiboye, Samanthia Johnson, Modoluwamu Idowu, Yarahy Leal, Taylor Sidney, Godstime Taiwo, Ibukun M. Ogunade, Christopher Ashwell

“…We examined the effects of a blend of live Saccharomyces cerevisiae, multiple probiotic bacteria, and their fermentation products on the whole blood transcriptome of newly weaned beef steers during a 56-d receiving period. …”
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GWAS and RNA-seq reveal novel loci and genes of low-nitrogen tolerance in cucumber (Cucumis sativus L.) by Huaxiang Wu, Huiming Yan, Bowen Li, Yike Han, Yike Han, Nan Liu, Mengyu Fan, Yahan Liu, Mingjie Lyu, Shengli Du, Shengli Du, Shengli Du, Aimin Wei, Aimin Wei

“…Through GWAS, we identified 29 QTNs harboring 196 candidate genes, while RNA sequencing (RNA-seq) revealed 3,765 differentially expressed genes (DEGs). 24 were identified by both methods. …”
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A Nextflow pipeline for T-cell receptor repertoire reconstruction and analysis from RNA sequencing data by Teresa Rubio, Maria Chernigovskaya, Susanna Marquez, Cristina Marti, Paula Izquierdo-Altarejos, Amparo Urios, Carmina Montoliu, Vicente Felipo, Ana Conesa, Victor Greiff, Sonia Tarazona

“…However, many non-targeted RNA-seq experiments also contain reads of TCR genes, which could be leveraged for TCR expression analysis while reducing sample requirements and costs. Moreover, a step-by-step pipeline for the processing of transcriptome RNA-seq reads to deliver immune repertoire data is missing, and these types of analyses are usually not included in RNA-seq studies of immunological conditions. …”
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Identification and validation of HOXC6 as a diagnostic biomarker for Ewing sarcoma: insights from machine learning algorithms and in vitro experiments by Yonghua Pang, Jiahui Liang, Jiahui Liang, Yakai Deng, Weinan Chen, Yunyan Shen, Jing Li, Xin Wang, Zhiyao Ren

“…Two-thirds of both the tumor and normal samples from the combined cohort were randomly selected for the training cohort, while the remaining one-third served as the internal validation cohort. …”
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Major facility superfamily sugar transporter protein SsMFSST1 regulates Sporisorium scitamineum mating, pathogenicity, and sugar transport/absorption by Yi Zhang, Yongding He, Meixin Yan, Shilong Zhang, Sisi Zhou, Wankuan Shen

“…The research results showed that the sexual mating ability of the knockout mutants significantly decreased compared with the wild type, while the sexual mating ability of the complementary mutants was almost restored to the level of the wild type. …”
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qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (<i>Litsea coreana</i>) by Qianli Dai, Min Lu, Ximeng Yang, Chenggong Lei, Feiyi Huang, Xueping Hu, Xin Huang, Xiaolong Nie, Daojing Chen, Sicheng Huang, Hengxing Zhu

“…<i>RPL</i> and <i>ACT</i> were the most stable genes across different leaf developmental stages, while <i>ACT</i> and <i>EF1-α</i> showed the highest stability across different leaf colors. …”
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Identification and Validation of Key Genes Involved in the Coupling of Mitochondria-Associated Endoplasmic Reticulum Membrane in Hemorrhoidal Disease by Mao L, Rao Z, Wang Y, Yang J, He J, Zheng Z, Chen L

“…Additionally, MUC16 was significantly overexpressed in patients with HD, while DEFA5 exhibited down-regulation compared to controls.Conclusion: This study identifies MUC16 and DEFA5 as key genes associated with HD and MAM and presents a predictive nomogram with high accuracy. …”
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Genetically supported causal genes for rheumatoid arthritis: Mendelian randomization and co‐localization analyses by Yuanyuan Niu, Fan Su, Simin Chen, Jingnan Wang, Shuoyang Zhang, Ruiru Li, Yu Kuang, Liuqin Liang, Youjun Xiao, Hanshi Xu

“…Results At a Bonferroni significance level of p < 2.70 × 10−6, the Mendelian randomization analysis revealed that 20 genes increased the risk of RA, while 16 genes showed a marginally protective effect. …”
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Analysis of CYP701A1 genes in gossypium species and functional characterization through gene silencing by Zhao Liang, Di Jiachun, Guo Qi, Xu Zhenzhen, Zhao Jun, Xu Peng, Xu Jianwen, Liu Jianguang, Shen Xinlian, Chen Xusheng

“…Conversely, salicylic acid content decreased, while the precursor for ethylene synthesis, 1-aminocyclopropane-1-carboxylic acid (ACC), remained relatively stable. …”
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Type 2C Protein Phosphatase MoPtc6 Plays Critical Roles in the Development and Virulence of <i>Magnaporthe oryzae</i> by Frankline Otieno Jagero, Abah Felix, Yakubu Saddeeq Abubakar, Meilian Chen, Wilfred M. Anjago, Hatungimana Mediatrice, Nkurikiyimfura Oswald, Osakina Aron, Wei Tang, Zonghua Wang, Jules Biregeya

“…Western blotting assay demonstrated that the phosphorylation level of MoOsm1 was decreased while MoMps1 was increased in the MoPtc6 deletion mutant, and comparative transcriptome assay revealed a higher number of expressed genes between mutant and wild type. …”
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Differential regulation of calcium-NFAT signaling pathway by Akt isoforms: unraveling effector dynamics and exhaustion of cytotoxic T lymphocytes in tumor microenvironment by Hung-Chih Yang, Li-Rung Huang, Ulrike Protzer, Wen-Ling Chen, Yong-Lin Chang, Su-Fang Lin, Masanori Isogawa

“…Conversely, Akt1 signaling triggered calcium influx and subsequent nuclear factor of activated T cells (NFAT) activation, while Akt2 signaling suppressed calcium influx, preventing excessive NFAT expression and nuclear translocation. …”
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NUDT21 lactylation reprograms alternative polyadenylation to promote cuproptosis resistance by Jinlong Lin, Yixin Yin, Jinghua Cao, Yiyang Zhang, Jiewei Chen, Rixin Chen, Bingxu Zou, Cijun Huang, Yongrui Lv, Shuidan Xu, Han Yang, Peng Lin, Dan Xie

“…While lactate is a well-established metabolic signal in cancer progression, its role in APA regulation remains unclear. …”
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Low light reduces saffron corm yield by inhibiting starch synthesis by Weijing Yang, Weijing Yang, Xin Li, Xin Li, Fei Chang, Fei Chang, Xue Qiu, Xulong Huang, Xulong Huang, Zhan Feng, Zhan Feng, Jie Yan, Jie Yan, Qinghua Wu, Qinghua Wu, Feiyan Wen, Feiyan Wen, Jin Pei, Jin Pei, Tao Zhou, Tao Zhou

“…LL also affected phytohormones concentrations, with increased levels of indole-3-acetic acid (IAA) and gibberellin (GA1) in LL leaves and daughter corms, and decreased abscisic acid (ABA) levels. Transcriptome and quantitative PCR analyses showed that LL upregulated the expression of genes involved in glycolysis and the tricarboxylic acid cycle in leaves, while downregulating CsSUS, CsINV1, CsAGPS1, CsZEP, and CsNCED, which are key to sucrose hydrolysis, starch synthesis, and ABA biosynthesis. …”
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Expression characteristics and functional identification of PpD2 gene in peach (Prunus persica) by XIE Hefang, ZHANG Yajia, ZHANG Junjie, LIAN Xiaodong, ZHANG Haipeng, CHENG Jun, WANG Wei, HOU Nan, WANG Lei, ZHENG Xianbo, FENG Jiancan, WANG Xiaobei, TAN Bin

“…Okubo is a typical standard cultivar with large branch angles and dense branching, while Sahonglongzhu is a pillar-type cultivar with smaller branch angles and fewer secondary branches. …”
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Analysis of abscisic acid-insensitive gene (ABI) sequence and phylogenetic relationship in genus Fagopyrum by WANG Yan, LIANG Chenggang, CHEN Qingqing, SHI Taoxiong, CHEN Qijiao, MENG Ziye, DENG Jiao, HUANG Juan, CHEN Qingfu

“…The ABI UniGene sequence of buckwheat was isolated from the previously performed transcriptome sequencing data (accession number: SRS1350375). …”
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Depletion of tumor-derived CXCL5 improves T cell infiltration and anti-PD-1 therapy response in an obese model of pancreatic cancer by Prabhakar Chalise, Richard McKinnon Walsh, Joseph Ambrose, Jarrid L Jack, Austin E Eades, Bailey A Bye, Mariana Tannus Ruckert, Fanuel Messaggio, Appolinaire A Olou, Dong Pei, Michael N VanSaun

“…CXC-ligands are a family of cytokines responsible for stimulating these receptors; while typically secreted by activated immune cells, fibroblasts, and even adipocytes, they are also secreted by immune-evasive cancer cells. …”
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