The Effect of <i>G0S2</i> Gene Knockout on the Proliferation, Apoptosis, and Differentiation of Chicken Preadipocytes by Yantao Li, Boyu Wang, Zhaochuan Wang, Jintian Wen, Tianle Zhou, Jiahao Tang, Zhenhui Li

“…To explore the involvement of <i>G0S2</i> in chicken preadipocyte growth and development, transcriptome sequencing was performed. The results demonstrated that <i>G0S2</i> was successfully deleted using the CRISPR/Cas9 system. …”
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Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization by Athena Boutou, Ilias Roufagalas, Katerina Politopoulou, Spyros Tastsoglou, Maya Abouzeid, Giorgos Skoufos, Laia Verdu de Juan, Jeong Hun Ko, Vasiliki Kyrargyri, Artemis G. Hatzigeorgiou, Christopher J. Barnum, Raymond J. Tesi, Jan Bauer, Hans Lassmann, Michael R. Johnson, Lesley Probert

“…Here, we show that microglia-specific deletion of tumor necrosis factor receptor 1 (TNFR1) and pharmacological inhibition of soluble TNF (solTNF) or downstream interleukin-1 receptor (IL-1R) allow maturation of highly activated disease-associated microglia with increased size and myelin phagocytosis capacity that accelerate cortical remyelination and motor recovery. Single-cell transcriptomic analysis of cortex at disease onset reveals that solTNF inhibition enhances reparative IL-10-responsive while preventing damaging IL-1-related signatures of disease-associated microglia. …”
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NKAPL facilitates transcription pause-release and bridges elongation to initiation during meiosis exit by Zhenlong Kang, Chen Xu, Shuai Lu, Jie Gong, Ruoyu Yan, Gan Luo, Yuanyuan Wang, Qing He, Yifei Wu, Yitong Yan, Baomei Qian, Shenglin Han, Zhiwen Bu, Jinwen Zhang, Xian Xia, Liang Chen, Zhibin Hu, Mingyan Lin, Zheng Sun, Yayun Gu, Lan Ye

“…Genetic variants in NKAPL are associated with azoospermia in humans, while mice carrying an NKAPL frameshift mutation (M349fs) show defective meiotic exit and transcriptomic changes similar to NKAPL depletion. …”
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Molecular and Biological Characterization of Novel Mitovirus Infecting Phytopathogenic Fungus Ustilaginoidea virens by He Zhenrui, Zhao Wenhua, Cheng Baoping, Yang Mei, Yang Yingqing, Zhu Yiming, Zhou Erxun

“…Activation or inhibition of autophagy through chemical experiments demonstrated that UvMV1 enhances viral titers by inducing autophagy in U. virens, while inhibition of autophagy results in decreased UvMV1 titers. …”
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Epigenetic modifier alpha-ketoglutarate modulates aberrant gene body methylation and hydroxymethylation marks in diabetic heart by Rohini Dhat, Dattatray Mongad, Sivarupa Raji, Silpa Arkat, Nitish R. Mahapatra, Nishant Singhal, Sandhya L. Sitasawad

“…Hyperglycemic myocardial microenvironment significantly alters chromatin architecture and the transcriptome, resulting in aberrant activation of signaling pathways in a diabetic heart. …”
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Cytochrome P450 2E1 aggravates DXR-induced myocardial injury through imbalanced mitochondrial OPA1 by Jiaxin Ma, Yaheng Wang, Huijiao Lv, Yu Lei, Feifei Guan, Wei Dong, He Wang, Lianfeng Zhang, Dan Lu

“…Results CYP2E1 overexpression accelerated, while CYP2E1 knockout inhibited, myocardial injury in DXR-induced cardiomyopathy and isoprenaline-induced hypertrophic cardiomyopathy. …”
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Proteomic analysis reveals chromatin remodeling as a potential therapeutical target in neuroblastoma by Zan Liu, Zitong Zhao, Longlong Xie, Zhenghui Xiao, Ming Li, Yong Li, Ting Luo

“…Results Our proteomic analysis reveals that pathways associated with the cell cycle and DNA replication are significantly upregulated in NB, while oxidative phosphorylation, pyruvate metabolism, and the TCA cycle are notably downregulated compared to GNB and GN. …”
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Widespread splicing changes in human brain development and aging by Pavel Mazin, Jieyi Xiong, Xiling Liu, Zheng Yan, Xiaoyu Zhang, Mingshuang Li, Liu He, Mehmet Somel, Yuan Yuan, Yi‐Ping Phoebe Chen, Na Li, Yuhui Hu, Ning Fu, Zhibin Ning, Rong Zeng, Hongyi Yang, Wei Chen, Mikhail Gelfand, Philipp Khaitovich

“…Abstract While splicing differences between tissues, sexes and species are well documented, little is known about the extent and the nature of splicing changes that take place during human or mammalian development and aging. …”
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Effect of m⁶A Recognition Protein YTHDC1 on Skeletal Muscle Growth by Huijun Huang, Geyan Lu, Liyao Xiao, Baohua Tan, Yuming Yang, Linjun Hong, Zicong Li, Gengyuan Cai, Ting Gu

“…Our results showed that YTHDC1 significantly promoted myogenic differentiation while inhibiting myoblast proliferation in C2C12 cells, and the results of our in vivo experiment showed that interfering with YTHDC1 led to a significant enhancement of muscle growth in mice. …”
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Integrative analysis of epigenetic subtypes in acute myeloid Leukemia: A multi-center study combining machine learning for prognostic and therapeutic insights. by Jincan Li, Shengyue Wang

“…High-risk patients exhibited distinct immune profiles with elevated M2 macrophages (1.8-fold) and Tregs (2.3-fold), while low-risk patients showed enhanced NK cell activity (2.1-fold). …”
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Gut microbiota dysbiosis orchestrates vitiligo-related oxidative stress through the metabolite hippuric acid by Qingrong Ni, Lin Xia, Ye Huang, Xiaoying Yuan, Weijie Gu, Yueqi Chen, Yijin Wang, Meng Nian, Shengxi Wu, Hong Cai, Jing Huang

“…Results Here, we compared transcriptome data of vitiligo lesions and normal skin and identified a high expression of oxidative stress-related genes in vitiligo lesions. …”
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Comprehensive Genome-Wide Characterization of L-Type Lectin Receptor-like Kinase (L-LecRLK) Genes in Wheat (<i>Triticum aestivum</i> L.) and Their Response to Abiotic Stress by Wan Zhao, Fuyan Zhang, Jiahuan Wang, Shuai Fang, Zhongjie Cheng, Xuhui Ma, Jialin Fan, Zhaoshi Xu, Xiaojie Chen

“…Gene duplication analysis revealed that both whole-genome/segmental and tandem duplications contributed to family expansion, while Ka/Ks ratio analysis suggested that the genes have undergone strong purifying selection. …”
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Study on the Regulatory Mechanisms of Carapace Marking Formation in <i>Marsupenaeus japonicus</i> by Panpan Wang, Jiawei Zhu, Huanyu Chen, Qingyuan Hu, Zhenxiang Chen, Wenjia Li, Ting Yang, Jin Zhu, Binlun Yan, Huan Gao, Chaofan Xing

“…In a previous study, we used comparative transcriptome technology to screen some functional genes related to body color regulation. …”
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FimC binds to the promoter region of agn43 to modulate autoaggregation by Zhihao Wang, Xiangpeng Niu, Ningyuan Zhong, Lanfang Kong, Saqib Nawaz, Haiyang Zhang, Wei Jiang, Yuanyuan Liu, Jian Tu, Xiangan Han

“…However, its regulatory role in agn43-mediated autoaggregation remains unclear.MethodsBy constructing fimC gene mutant strains, the autoaggregation, motility, biofilm formation, and the adhesion and invasion ability to HD-11 cells were examined. The transcriptome and the electrophoretic mobility shift assay (EMSA) were used to screen and verify the regulation of fimC on downstream genes.ResultsThe results demonstrated that the lack of fimC, but not fimbriae, significantly increased autoaggregation (p &lt; 0.001) while promoting the transcription of agn43 (p &lt; 0.01). …”
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Ethyl β-carboline-3-carboxylate targets PRDX5/c-Jun axis for novel therapeutic strategy against cervical cancer by Wan-Qiu Xiao, Dong-Hun Lee, Hakjin Kim, Hui-Na Zhang, Yao-Yuan Feng, Ying-Hua Jin, Mei-Hua Jin, Ying-Hao Han, Taeho Kwon, Hu-Nan Sun

“…Notably, β-CCE treatment promotes apoptosis through c-Jun up-regulation, with PRDX5 knockdown enhancing this effect while its overexpression provides protection. In xenograft mouse models, β-CCE treatment significantly suppressed tumor growth and enhanced anti-tumor immune responses, particularly in PRDX5-depleted conditions, without apparent systemic toxicity. …”
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OsNUDX23 regulates early seed germination by modulating ROS balance and starch metabolism in rice by Xing Dai, Xing Dai, Wenwen Kong, Feiyan Wu, Zijie Pan, Lei Gao, Beixin Mo, Yu Yu, Weigui Luo

“…Reactive Oxygen Species (ROS) play a dual role during seed germination: at low concentrations, they function as signaling molecules to facilitate germination, while at high levels, they can induce oxidative damages. …”
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Genome concentration limits cell growth and modulates proteome composition in Escherichia coli by Jarno Mäkelä, Alexandros Papagiannakis, Wei-Hsiang Lin, Michael Charles Lanz, Skye Glenn, Matthew Swaffer, Georgi K Marinov, Jan M Skotheim, Christine Jacobs-Wagner

“…In bacteria, ribosome concentration is known to be a constraining factor of cell growth rate, while gene concentration is usually assumed not to be limiting. …”
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Epitranscriptomic Analysis of the Ventral Hippocampus in a Mouse Model of Post-Traumatic Stress Disorder Following Deep Brain Stimulation Treatment of the Basolateral Amygdala by Mingxi Ma, Hao Fan, Hui Zhang, Yao Yin, Yizheng Wang, Yan Gao

“…Gene ontology (GO) enrichment analysis revealed that FS altered methylation in metabolic, developmental, and cytoskeletal pathways, while BLA DBS targeted metabolic, cell cycle, and neuroplasticity-related genes. …”
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Transcriptional Sequencing and Gene Expression Analysis of Various Genes in Fruit Development of Three Different Black Pepper (Piper nigrum L.) Varieties by Choy Yuen Khew, Jennifer Ann Harikrishna, Wei Yee Wee, Ee Tiing Lau, Siaw San Hwang

“…Comparison of flower and fruit transcriptome data from the three different black pepper varieties revealed a large number of DEGs between flower and fruit of the SA variety. …”
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An ovarian insulin-like peptide specifically regulates energy allocation and oocyte development in nutrition-restricted cockroaches by Yuan Luo, Wen-Xin Hou, Shan-Shan Zhao, Yun-Long Cheng, Ke-Jia Zhang, Liang-Guan Lin, Sheng Li, Xiao-Jin Pei

“…Repression of BgILP2 impairs vitellogenesis in the fat body through downregulation of p-AKT and p-ERK levels while simultaneously disrupting juvenile hormone synthesis, ultimately leading to delayed oocyte development under nutrient restriction. …”
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