MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11 by Yi Li, Jiayuan Fang, Yingying Jiao, Qinchuan Lv, Xingyu Xiao, Shuo Zheng, Xi Chen, Jie Song, Xunming Zhang, Libo Zhang, Ze Ma, Changhong Li, Linlin Hao

“…Results The analysis of porcine BodyMap transcriptome data revealed differential expression of miRNA 21-5p in skeletal muscle and adipose tissue. …”
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Genome-wide characterization and expression profiling of MADS-box family genes during organ development and drought stress in Camelina sativa L by Somayeh Tahmasebi, Parisa Jonoubi, Mohammad Majdi, Ahmad Majd, Parviz Heidari

“…The predicted molecular weights (MW) of the CsMADS-box proteins displayed a range from 17.01 to 94.06 kDa, while the isoelectric points (pI) were observed to span from 4.13 to 10.09. …”
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Neuronal CDK5RAP3 deficiency leads to encephalo-dysplasia via upregulation of N-glycosylases and glycogen deposition by Fanghui Chen, Minghui Xiang, Zhipeng Wang, Fan Yang, Junzhi Zhou, Zihan Deng, Susu Wang, Ping Li, Jieqi Tew, Wei Zhang, Honglin Li, Yong Teng, Xiaobin Zhu, Yafei Cai

“…Morphological analysis demonstrated that neuronal CDK5RAP3 deficiency leads to increased SLC17A6 and N-glycosylase (RPN1 and ALG2) protein expression, and while causing endoplasmic reticulum (ER) stress. …”
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Expression of sex-related genes and effects of dmrt1 siRNA-silencing on sex differentiation in juvenile yellow perch by Ding-Kun Xie, Han-Ping Wang, Rafidah Othman, Hong Yao, Paul O’Bryant, Dean Rapp

“…Then an experiment was conducted to determine the effects of dmrt1 siRNA-silencing on sex differentiation of juvenile YP. Transcriptome analysis showed that dmrt1 were mainly expressed in gonad tissue with their levels being significantly higher in the male groups respectively comparing to each other; foxl2 was dominantly expressed in gonads showing a significantly higher expression in big female groups than the male and small female groups. cyp19a1 was dominantly expressed in gonads, while cyp19a2 was dominantly expressed in brain, and both female groups exhibiting a significantly higher expression than the male groups. sox9a was mainly expressed in brain and gonad tissue with the male groups displaying a significantly higher expression in gonad than the female groups. …”
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Genome-wide identification of invertase genes in sweetpotato and its response to nitrogen and planting densities by Zheng-yu Hu, Pei-xin He, Yu-jie Li, Hong-Juan Liu, Rafiq Ahmad, Izhar Ali, Cheng-cheng Si

“…Gene Ontology functional and protein interaction network analysis suggested that IbCWIN1/2/5/6 potentially interact with HKL1/3, HXK2/3/4, and other proteins, significantly influencing carbohydrate metabolic functions and biological processes in sweetpotato. Transcriptome data revealed that IbCIN2 and IbVIN3 were highly expressed in fibrous root (FR) and potential storage root (PSR), while IbVIN4 exhibited high expression levels in storage roots (SR), and IbCWIN2 was highly expressed in both FR and SR. …”
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SK4 potentially modulates the alternative splicing profile associated with papillary thyroid cancer development in BHT101 cells by Alimujiang Wupuer, Xuekelaiti Kuerban, Gong Ye

“…Recent studies demonstrated the potassium channel protein SK4 participates in the progression of many cancers, while it lacks the molecular mechanism study for SK4 function. …”
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QTL mapping provides new insights into emamectin benzoate resistance in salmon lice, Lepeophtheirus salmonis by Armin Sturm, Greta Carmona-Antoñanzas, Joseph L. Humble, Claudia Croton, Sally Boyd, Rapule Mphuti, John B. Taggart, David I. Bassett, Ross D. Houston, Karim Gharbi, James E. Bron, Michaël Bekaert

“…The avermectin emamectin benzoate (EMB) is widely used for salmon delousing. While resistance to EMB is widespread in Atlantic populations of L. salmonis, the molecular mechanisms of resistance remain to be elucidated. …”
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Role of Maternal Obesity in Offspring Cardiovascular Development and Congenital Heart Defects by Ashleigh McMullan, James B. Zwierzynski, Nina Jain, Laura S. Haneline, Weinian Shou, Kok Lim Kua, Swetansu K. Hota, Matthew D. Durbin

“…The global prevalence of obesity is rising, and while maternal obesity is a known risk factor for fetal congenital heart disease, the specific mechanisms are largely unexplored. …”
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Functional characterization of COMT genes in Chinese white pear (Pyrus bretschneideri) and their role in lignin synthesis by Xiaofeng Feng, Xiaofeng Feng, Xianang Wu, Xianang Wu, Jiayi Hong, Jiayi Hong, Zihui Guo, Zihui Guo, Yasmin Khan, Yasmin Khan, Dongyi Wei, Dongyi Wei, Honghong Fan, Honghong Fan, Yongping Cai, Yongping Cai

“…These genes exhibit five conserved motifs, and promoter analysis indicates potential hormonal regulation. Transcriptome data showed that PbrCOMT1 is the predominant COMT gene in ‘Dangshan Su’ fruit and is likely crucial for lignin synthesis. …”
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QTL-based dissection of three key quality attributes in maize using double haploid populations by Zitian He, Jianping Wang, Jianping Wang, Jialei Li, Jianwei Li, Lei Chen, Xiaolei Zhang, Xiaolei Zhang

“…Understanding the genetic basis of these quality traits significantly contributes to improving maize yield and optimizing end-use quality. While previous studies have explored the genetic basis of these traits, further investigation into the quantitative trait loci (QTL) responsible for variations in starch content, oil content, and lysine content still requires additional attention.MethodsDouble haploid (DH) populations were developed via a nested association mapping (NAM) design. …”
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Identification of the metabolic protein ATP5MF as a potential therapeutic target of TNBC by Kaiyan Chen, Yingchun Wu, Linfeng Xu, Changyong Wang, Jinqiu Xue

“…Methods For this research, the scRNA data was obtained from the GEO database, while the transcriptome data was obtained from the TCGA and METABRIC databases. …”
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Photothermal therapy improves the efficacy of topical immunotherapy against melanoma by Lei Shi, Fuhe Zhang, Jia Yan, Min Luo, Kaili Liu, Pei Liu, Guorong Yan, Chunxiao Li, Yutong Yang, Qingyu Zeng, Guolong Zhang, Wei R. Chen, Xiuli Wang

“…Additionally, the population of mature dendritic cells, CD4+ T cells, and CD8+ T cells were increased, while myeloid-derived suppressor cells were downregulated after LIT. …”
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Genome characterization of a newly discovered grapevine leafroll-associated virus S, in the genus ampelovirus by high-throughput sequencing by Malyaj R. Prajapati, Nitika Gupta, Mailem Yazing Shimray, Jayesh Gehlot, Abhinav Tiwari, Pooja Thapa, Damini Diksha, Somnath Kadappa Holkar, Pallavi Jagannath Mahajan, Sujoy Saha, Virendra Kumar Baranwal, Susheel Kumar Sharma

“…Background: Transcriptome data obtained from plant samples often contain significant numbers of reads originating from viral genomes, which are typically co-isolated during the RNA extraction process. …”
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Early-life exercise extends healthspan but not lifespan in mice by Mengya Feng, Min Li, Jing Lou, Guiling Wu, Tian Gao, Fangqin Wu, Yanzhen Tan, Nini Zhang, Yong Zhao, Lin Zhao, Jia Li, Changhong Shi, Xing Zhang, Jiankang Liu, Feng Gao

“…Multiple-organ transcriptome analyses identified enhanced fatty acid metabolism in skeletal muscles as a major feature in aged mice that underwent early-life exercise. …”
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DNA methylation memory of pancreatic acinar-ductal metaplasia transition state altering Kras-downstream PI3K and Rho GTPase signaling in the absence of Kras mutation by Emily K.W. Lo, Adrian Idrizi, Rakel Tryggvadottir, Weiqiang Zhou, Wenpin Hou, Hongkai Ji, Patrick Cahan, Andrew P. Feinberg

“…During ADM, digestive enzyme-producing acinar cells acquire a transient ductal epithelium-like phenotype while maintaining their geographical acinar organization. …”
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Mechanisms of sorafenib-induced cardiotoxicity: ER stress induces upregulation of ATF3, leading to downregulation of NDUFS1 expression and mitochondrial dysfunction by Ming Yan, Ming Yan, Ming Yan, Cheng Peng, Cheng Peng, Xueyan Lang, Xueyan Lang, Yilan Li, Yilan Li, Yao Zhang, Yao Zhang

“…Further enrichment analysis identified several related pathways, while RT-PCR and immunoblotting confirmed ATF3 upregulation in H9C2 cells. …”
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Investigation of the Effects of Salinity Exposure on Immune Defense, Morphology, and Gene Expression in the Gills of <i>Macrobrachium nipponense</i> by Shubo Jin, Rong Zhou, Hongtuo Fu, Wenyi Zhang, Hui Qiao, Yiwei Xiong, Sufei Jiang

“…Lysosome, apoptosis, amino sugar, and nucleotide sugar metabolism and the cGMP-PKG signaling pathway are immune-related metabolic pathways, while pancreatic secretion is an energy-metabolism-related metabolic pathway, suggesting that the immune response and energy metabolism play essential roles in the regulation of saline acclimation in this species. …”
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Spatial and single cell mapping of castleman disease reveals key stromal cell types and cytokine pathways by David Smith, Anna Eichinger, Éanna Fennell, Zijun Y. Xu-Monette, Andrew Rech, Julia Wang, Eduardo Esteva, Arta Seyedian, Xiaoxu Yang, Mei Zhang, Dan Martinez, Kai Tan, Minjie Luo, Katherine J. Young, Paul G. Murray, Christopher Park, Boris Reizis, Vinodh Pillai

“…MCD is characterized by increased TRC while UCD shows increased B-reticular cells (BRC). …”
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Genome-Wide Identification of <i>UGT</i> Genes and Analysis of Their Expression Profiles During Fruit Development in Walnut (<i>Juglans regia</i> L.) by Danhua Shi, Jinyu Yang, Gengyang Li, Yuanting Zhou, Pei Yao, Yanyu Shi, Jieyun Tian, Xiaojun Zhang, Qunlong Liu

“…Furthermore, motif annotation confirmed that motifs 1, 2, and 3 represented conserved structural features within <i>UGT</i> proteins, while interestingly, around 56.5% of walnut <i>UGT</i> members lacked introns. …”
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Dietary effects of astaxanthin on gonadal development in female broodstock of Macrobrachium rosenbergii by Mingwei Tao, Jie Wei, Clement de Cruz, Junyi Wang, Houkuan Du, Hangxian Zhou, Qiyou Xu

“…The AS62 group exhibited the highest gonadosomatic index and estradiol levels, while vitellogenin levels were elevated in AS62 and AS140 (P < 0.05). …”
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