The systematic analysis of genes related to butyrate metabolism suggests that CDKN3 could serve as a promising therapeutic target for lung adenocarcinoma treatment by Yanchao Luan, Chao Liang, Qingsong Han, Xueqin Zhou, Na Yang, li Zhao

“…Additionally, the risk score showed a positive correlation with TIDE and Exclusion score, while showing a negative correlation with Dysfunction score. …”
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Insights into the senescent mechanisms of harvested strawberry fruit at the physiological, molecular and metabolic levels by Boqian Yan, Ye Wang, Yuan Bai, Ziyang Liu, Huijun Liu, Xiangning Chen, Yuanyue Shen, Liusheng Duan

“…Therefore, we performed a series of morphological, physiological and biochemical, as well as transcriptome and proteome analyses using the widely-cultivated strawberry 'Benihoppe' during 0−8 d at room temperature. …”
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Smoking-Associated Changes in Gene Expression in Coronary Artery Disease Patients Using Matched Samples by Mohammed Merzah, Szilárd Póliska, László Balogh, János Sándor, Szilvia Fiatal

“…Next-generation sequencing was employed using the NextSeq 500 system to generate high-throughput sequencing data for transcriptome profiling. The differentially expressed genes were analyzed using the R programming language. …”
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A gene expression atlas of Nicotiana tabacum across various tissues at transcript resolution by Shizhou Yu, Jufen Wan, Jufen Wan, Tenghang Xu, Tenghang Xu, Jie Zhang, Linggai Cao, Jie Liu, Hongfeng Liu, Xueliang Ren, Zhixiao Yang

“…Alternative splicing (AS) expands the transcriptome diversity by selectively splicing exons and introns from pre-mRNAs to generate different protein isoforms. …”
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Role of LncRNA MRPS28 in Secondary Hair Follicle Development of Cashmere Goats by Youjun Rong, Rong Ma, Qing Ma, Bingjie Ma, Xuxu Bao, Yiming Zhang, Le Wang, Fangzheng Shang, Ruijun Wang, Rui Su, Yu Wang, Yanjun Zhang

“…Additionally, it can inhibit the proliferation and migration of dermal fibroblasts while promoting their apoptosis. Further mechanistic studies have shown that lncRNA MRPS28 acts as a competing endogenous RNA, sponging chi-miR-145-5p, thereby relieving its inhibitory effect on the target gene <em>NUAK1</em>. …”
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Targeting NF-kappaB-inducing kinase shapes B-cell homeostasis in myasthenia gravis by Xiaoyu Huang, Zhouao Zhang, Zhouyi Wang, Tiancheng Luo, Mingjin Yang, Xinyan Guo, Xue Du, Tianyu Ma, Yong Zhang

“…Therefore, we aimed to identify potential novel therapeutic targets that preserve part of B cell function while inhibiting antibody-secreting cells (ASCs). …”
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Evidence from a Comprehensive Bioinformatics Analysis Point to Possible Therapeutic Targets for Vitiligo by Wang Y, Yao T, Lin Y, Wu J

“…Colocalization analysis indicated that these targets share common variants with vitiligo, while PheW-MR analysis suggested that targeting these proteins would not result in significant side effects. …”
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Downregulation of hsa-miR-135b-5p Inhibits Cell Proliferation, Migration, and Invasion in Colon Adenocarcinoma by Yunxin Zhang, Wentao Zhang, Wenlong Xia, Junwei Xia, Haishan Zhang

“…A total of 3384 DEmRNAs were screened, and enrichment analysis showed that the upregulated mRNAs were enriched in 25 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and 326 Gene Ontology Biological Processes (GO-BPs) while the downregulated mRNAs were enriched in 20 KEGG pathways and 276 GO-BPs. …”
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Transcriptional regulation of ETV5 by mitogen-activated protein kinase via ETS-1 in human pancreatic cancer cells by Aya Noguchi, Masanobu Kimura, Yuriko Saiki, Tomohiko Ishikawa, Takashi Kokumai, Yuko Omori, Yusuke Ono, Yusuke Miszukami, Masaharu Ishida, Masamichi Mizuma, Kei Nakagawa, Michiaki Unno, Toru Furukawa

“…Investigation of functional significances of ETS variant transcription factor 5 (ETV5) expression in the pancreatic cancer cells revealed that ETV5 was associated with resistance to gemcitabine; while no significance in proliferation, migration, and invasion. …”
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PIEZO1 promotes psoriasis-like skin inflammation in mice via NF-κB/IL-17 signaling pathway activation by Wen Li, Kan Ze, Xufeng He, Lili Yang, Huimin Zhang, Weian Yuan, Wuqing Wang

“…Loss of PIEZO1 in vitro inhibited keratinocyte proliferation and migration, while inducing apoptosis. Transcriptome sequencing and subsequent analyses revealed that PIEZO1 knockdown modulates the NF-kB signaling pathway and associated inflammatory genes. …”
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Targeting PDK1: A novel approach to combat hypoxia‐induced epithelial‐mesenchymal transition in chronic rhinosinusitis with nasal polyps by Sicen Pan, Mengyan Zhuang, Xiangdong Wang, Qinqin Zhang, Ting He, Ying Li, Jian Jiao, Luo Zhang

“…PDK1 overexpression or lactate stimulation also triggered EMT, while PDK1 inhibition attenuated hypoxia‐induced EMT in HNECs. …”
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Preoperative short-term fasting in paediatric cancer care is safe, feasible and activates protective responses by D.S.J. Komninos, C.A.J. Oudmaijer, R.A. Ozinga, K. Smit, J.N.M. Ijzermans, J.H.J. Hoeijmakers, R.C. Minnee, M.M. van den Heuvel-Eibrink, W.P. Vermeij

“…This study aims to explore the safety and feasibility of incorporating STF into the treatment regimen for children with renal cancer, while simultaneously showing a protective transcriptomic signature. …”
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Mitigating microplastic toxicity: How particle size and degrading bacteria influence Cucumis sativus L. seedlings by Shuyi Chen, Peng Wu, Hao Ren, Guohua Shen, Zhuang Xiong, Wenlin Zhao, Xiang Guo, Peng Xiang, Junqiang Xu, Qiang Li

“…Transcriptome analysis further revealed that the PS-MPs treatment affected the biosynthesis process of cucumber seedlings, while treatment with degrading bacteria significantly increased root synthesis and metabolism in the plant. …”
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The Molecular Mechanism by Which miR-211-5p Regulates the Proliferation and Differentiation of Preadipocytes in Meat Rabbits by Targeting TPK1 by Xiaoxiao Zhang, Meigui Wang, Tao Tang, Jing Zhou, Wenqiang Sun, Xianbo Jia, Jie Wang, Hengwei Yu, Songjia Lai

“…RT-qPCR, western blot (WB), and Oil Red O staining assays showed that overexpression of miR-211-5p promotes the maturation and differentiation of precursor adipocytes in meat rabbits, while inhibition of miR-211-5p expression inhibits the maturation and differentiation of precursor adipocytes in rabbits. (3) Through transcriptome sequencing, a total of 147 differentially expressed genes were identified. …”
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RNA multi-omics in single cells reveal rhythmical RNA reshaping during human and mouse oocyte maturation by Huan Yao, Danru Zhang, Haixia Jin, Yanjie Guo, Yan Liu, Shengnan Wang, Tong Li, Shenli Yuan, Gang Lu, Yingpu Sun

“…Abstract Background Omics technologies are widely applied in assisted reproductive technology (ART), such as embryo selection, investigation of infertility causes, and mechanisms underlying reproductive cell development. While RNAomics has shown great potential in investigating the physiology and pathology in female reproductive system, its applications are still not fully developed. …”
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Identification and expression analysis of ACBPs family members of Hippophae rhamnoides subsp. sinensis Rousi in response to lead stress by CHEN Ke, LI Xinjuan, ZHANG Tian, REN Qiandan, SUN Jing, ZHOU Wu

“…Protein 3D structure predictions revealed that Group 3 proteins were the most complex with high model similarity; Group 1 proteins were simpler but also similar; Group 4 proteins showed low similarity and large differences. Transcriptome analysis and qRT-PCR validation indicated that, except for HrLACBPs1, the other genes had lower expression levels at 2000 mg·kg-1 lead compared to controls, while their expression increased at 5000 mg·kg-1 lead. …”
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Effect and mechanism of sodium butyrate on the activation of Epstein-Barr virus in Epstein-Barr virus-positive gastric cancer cells by TIAN Zhaoxin, CHEN Yuying, LIU Wen

“…Methods EBV-positive gastric cancer cells (AGS-EBV cells) were divi-ded into groups A, B, and C, and the cells in group A were cultured routinely for 12 hours, while those in groups B and C were treated with NaB for 12 and 24 hours respectively. …”
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Unveiling the Temporal Dynamics and Molecular Regulation Profiles of Neutrophil Extracellular Traps Following Spinal Cord Injury by Li J, Chang C, Li Y, Cui S, Bai J, Zhang C, Wang X, Li K, Jian F

“…WGCNA identified SCI-related modules, while GSVA assessed NET formation dynamics. Publicly available single-cell RNA sequencing data from the GEO database determined cell-specific expression patterns of key NRGs. …”
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Investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in Lavandula angustifolia Mill. under cold stress by Yuchen Liang, Yuchen Liang, Yuchen Liang, Yuchen Liang, Yinan Liu, Yinan Liu, Yu Wang, Yu Wang, Ruijiao Yang, Ruijiao Yang, Zening Yuan, Zening Yuan, Hui Li, Hui Li

“…To investigate the mechanisms underlying cold tolerance in L. angustifolia and support economic development, we measured the sugar content and performed transcriptome analysis at temperatures of 30°C (control), 20°C, 10°C, and 0°C. …”
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Effects of Genetic Polymorphism in the <i>IFI27</i> Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells by Xinyi Jiang, Zhihui Zhao, Xuanxu Chen, Fengshuai Miao, Jing Li, Haibin Yu, Ping Jiang, Ziwei Lin

“…Haplotype frequency analysis showed that H5H5 was associated with lower milk fat content (<i>p</i> < 0.05), while milk from H5H6 animals had a higher fat content (<i>p</i> < 0.05). …”
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