Autoantibodies Targeting Vinculin Reveal Novel Insight into the Mechanisms of Autoimmune Podocytopathies by Hanyan Meng, Dongjie Wang, Chen Zheng, Chao Zhou, Xinrui Mao, Jinglan Gu, Xiaohui Qiao, Fei Liu, Jingjing Wang, Haidong Fu, Jianhua Mao, Qing Ye

“…Mouse experiments revealed that injecting anti-vinculin antibodies or recombinant vinculin protein induced proteinuria and podocyte injury in the immunized mice, and the renal phenotype closely resembled the pathological characteristics of minimal change disease. Transcriptome sequencing of renal tissues revealed up-regulation of inflammation, immune responses, cytokine activities, and B cell activation pathways in the immunized mice, while cytoskeleton-related functions were down-regulated. …”
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Scorpion venom peptides enhance immunity and survival in Litopenaeus vannamei through antibacterial action against Vibrio parahaemolyticus by Ling Zeng, Yulin Sun, Hualin Zhang, Xiangxi Yi, Ran Du, Ziming Chen, Qi Wang

“…Furthermore, intestinal transcriptome profiling was performed to identify the activated immune pathways.ResultsAll peptides exhibited membrane-targeting activity: BmKn2-7 showed superior outer membrane penetration and depolarization, while BmKn1 was more effective in inner membrane disruption and ROS induction. …”
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Multi-level regulation of hindgut homeostasis by volatile fatty acid administration in dairy goats: linking microbial metabolism to epithelial inflammation and barrier function by Yongkang Zhen, Ali Rahmat, Chong Zhang, Jiaqi Lin, Jianjun Ma, Yuhong Zhong, Mengzhi Wang

“…SB reduced serum malondialdehyde levels (P = 0.042), while SA and SP increased intestinal interleukin-10 concentration compared with CON (P < 0.001). …”
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Decoding ARF4 and EIF5B-Based Prognostic Signatures and Immune Landscape Following Insufficient Radiofrequency Ablation in Hepatocellular Carcinoma: Through Multi-Omics and Experim... by Zhang Y, Lu Y, Zheng S, Luo W, Tan M, Luo B

“…Experimental validations confirmed the protective role of ARF4 and EIF5B in IRFA outcomes, while knockdown experiments suggested their involvement in promoting cell proliferation, migration, invasion, and EMT in IRFA models, potentially through pathways like P53 and Transforming Growth Factor Beta(TGF-β) signaling pathway activation as indicated by transcriptome sequencing.Conclusion: ARF4 and EIF5B have demonstrated promising potential as biomarkers influencing patient prognosis following RFA in HCC. …”
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Injectable and Assembled Calcium Sulfate/Magnesium Silicate 3D Scaffold Promotes Bone Repair by In Situ Osteoinduction by Wei Zhu, Tianhao Zhao, Han Wang, Guangli Liu, Yixin Bian, Qi Wang, Wei Xia, Siyi Cai, Xisheng Weng

“…In vivo results confirmed enhanced trabecular density and bone volume compared to controls, indicating effective bone repair and regeneration. (4) Conclusions: The Mg@Ca scaffold offers a promising therapeutic approach for ONFH, providing a minimally invasive solution for bone defect repair while stimulating natural bone regeneration. Its injectable and self-setting properties ensure precise filling of bone defects, making it suitable for clinical applications.…”
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Impaired hippocampal neurogenesis associated with regulatory ceRNA network in a mouse model of postoperative cognitive dysfunction by Jingrun Lin, Xiaoqiu Zhu, Xuan Li, Yu Hong, Yaohui Liang, Siqi Chen, Chenzhuo Feng, Lin Cao

“…The surgery group underwent laparotomy under 1.5% isoflurane anesthesia, while controls received no intervention. Cognitive function was assessed 7–10 days post-surgery using open field, Y-maze, and novel object recognition tests. …”
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Role and mechanism of action of nuclear receptor coactivator 4 in the development of acquired resis-tance to targeted inhibitors in pancreatic cancer with KRAS G12C mutation by CHANG Hao, WANG Ya'nan, LIU Shihai, WANG Jing

“…Res-MIA cells were divided into groups a and d, while Res-MIA-KD cells were divided into groups b and c; the cells in groups c and d were treated with adagrasib (2 μmol/L), while those in groups a and b received no drug treatment. …”
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Inflammasome sensor NLRP1 controls rat macrophage susceptibility to Toxoplasma gondii. by Kimberly M Cirelli, Gezahegn Gorfu, Musa A Hassan, Morton Printz, Devorah Crown, Stephen H Leppla, Michael E Grigg, Jeroen P J Saeij, Mahtab Moayeri

“…We show here that rat strain differences in Toxoplasma infected macrophage sensitivity to pyroptosis, IL-1β/IL-18 processing, and inhibition of parasite proliferation are perfectly correlated with NLRP1 sequence, while inversely correlated with sensitivity to anthrax LT-induced cell death. …”
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The regulatory roles of small nucleolar RNAs within their host locus by Étienne Fafard-Couture, Stéphane Labialle, Michelle S Scott

“…Besides this canonical role, several snoRNAs are now known to regulate diverse levels of gene expression. While these functions are carried out in trans by mature snoRNAs, evidence has also been emerging of regulatory roles of snoRNAs in cis, either within their genomic locus or as longer transcription intermediates during their maturation. …”
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mRNA stability fine-tunes gene expression in the developing cortex to control neurogenesis. by Lucas D Serdar, Jacob R Egol, Brad Lackford, Brian D Bennett, Guang Hu, Debra L Silver

“…We use SLAM-seq to measure RNA half-lives transcriptome-wide across multiple stages of cortical development. …”
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Identification of stable reference genes for qRT-PCR in Stropharia rugosoannulata using mRNA-sequencing data. by Xiaohua Zhang, Xuesong Li, Rong Hua, Yuan Fang, Tingsong Yue, Jianying Li, Yuxun Lu, Wansong Yue, Zhanghui Gao, Shaoxiong Liu, Dafeng Sun

“…The results indicated that UBP exhibited the highest stability across various developmental stages of red and yellow S. rugosoannulata, while RPB2 and GAPDH showed the least stability. …”
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Mycoviruses: trends in plant-fungus-mycovirus interactions and ‘biocontrol’ prospects in agriculture and the environment by Elias Mjaika NDIFON, Gilbert Nchongboh CHOFONG

“…For instance, grazer viruses completely oxidize the organic content of their host into carbon dioxide and inorganic nutrients, while lytic viruses release the organic material from their hosts without modification. …”
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Neighbourhood continuity is not required for correct testis gene expression in Drosophila. by Lisa A Meadows, Yuk Sang Chan, John Roote, Steven Russell

“…We compare gene expression in individuals carrying inverted chromosomes with their non-inverted but otherwise identical progenitors using whole-transcriptome microarray analysis, validating these data with specific quantitative real-time PCR assays. …”
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Toxicity Responses from Tributyltin Chloride on Haarder (<i>Planiliza haematocheila</i>) Livers: Oxidative Stress, Energy Metabolism Dysfunction, and Apoptosis by Changsheng Zhao, Anning Suo, Dewen Ding, Wencheng Song

“…Furthermore, we discovered that blood reactive oxygen species rose while total blood cell count decreased. As malondialdehyde levels rose, total antioxidant capacity and antioxidant enzyme activity (superoxide dismutase, catalase, and glutathione peroxidase) were markedly reduced. …”
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Integrative genomic analysis identifies isoleucine and CodY as regulators of Listeria monocytogenes virulence. by Lior Lobel, Nadejda Sigal, Ilya Borovok, Eytan Ruppin, Anat A Herskovits

“…Intracellular bacterial pathogens are metabolically adapted to grow within mammalian cells. While these adaptations are fundamental to the ability to cause disease, we know little about the relationship between the pathogen's metabolism and virulence. …”
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Intratracheal Aerosolization of <i>Nocardia farcinica</i> in Mice Optimizes Bacterial Distribution and Enhances Pathogenicity Compared to Intranasal Inoculation and Intratracheal I... by Bingqian Du, Ziyu Song, Jirao Shen, Jiang Yao, Shuai Xu, Xiaotong Qiu, Min Yuan, Zhenjun Li

“…Intranasal inoculation (IN) is the traditional approach for constructing a <i>Nocardia</i>-induced pneumonia mice model, while it usually only results in limited local bacterial infection in the lungs. …”
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Nurr1 deficiency impairs autophagy-lysosomal function through GBA-dependent transcriptional regulation in Parkinson’s disease pathogenesis by Cheng Cheng, Cheng Cheng, Huijia Yang, Lulu Tian, Yang Ni, Congcong Jia, Weidong Le, Qingshan Wang

“…Transcriptome sequencing and analysis was utilized to confirm the altered pathways and differentially expression genes associated with ALP. …”
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Multi-omics data reveal that SAA1 + fibroblasts exacerbate periodontitis by regulating macrophage inflammation and chemotaxis by Li Li, Yuting Yang, Fang Dai, Libin Deng, Meixiu Jiang, Chenjiang He, Ting Long, Kaiqiang Yang, Xinbo Yang, Li Song

“…Methods Through integrative analysis of single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, and bulk transcriptome datasets from periodontal tissues, we systematically characterized the spatial architecture and intercellular communication networks within the inflammatory periodontal microenvironment, identifying a functionally serum amyloid A1 + fibroblasts (SAA1 + Fib) that critically drives disease progression. …”
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A three-component signaling system plays multiple roles in the pathogenicity of Xanthomonas citri subsp. citri by Xiaoyan Zhao, Xiaoyun Chen, Junjie Lei, Jiaying Feng, Yunyang Dong, Yu Shi, Linghui Xu, Junxia Wang

“…The subcellular localization of XAC1991, XAC1992, and XAC1994 was visualized using Leica microscopy, while the flagellar structure of each mutant strain was examined by transmission electron microscopy. …”
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Transcription of a centromere-enriched retroelement and local retention of its RNA are significant features of the CENP-A chromatin landscape by B. J. Chabot, R. Sun, A. Amjad, S. J. Hoyt, L. Ouyang, C. Courret, R. Drennan, L. Leo, A. M. Larracuente, L. J. Core, R. J. O’Neill, B. G. Mellone

“…Abstract Background Centromeres depend on chromatin containing the conserved histone H3 variant CENP-A for function and inheritance, while the role of centromeric DNA repeats remains unclear. …”
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