Exploring the Efficacy and Potential Mechanisms of Topical <i>Periplaneta americana</i> (L.) Extract in Treating Androgenetic Alopecia in a Mouse Model: A Systems Pharmacology and... by Tangfei Guan, Xin Yang, Canhui Hong, Peiyun Xiao, Yongshou Yang, Chenggui Zhang, Zhengchun He

“…Concurrently, PA-011 activated the proliferative potential of hair follicle stem cells, as demonstrated by upregulated expression of the cell proliferation marker Ki67, and activated the Wnt/β-catenin signaling pathway in DHT-induced AGA mice. Transcriptomic and metabolomic analyses revealed multi-target effects of PA-011, including modulation of PI3K-Akt/MAPK pathways, pentose phosphate metabolism, and amino acid biosynthesis. 16S rRNA sequencing and metagenomic analysis showed that AGA disrupts skin microbial homeostasis, while PA-011 intervention normalized the microbiota composition. …”
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Differentially expressed ncRNAs as key regulators in infection of human bronchial epithelial cells by the SARS-CoV-2 Delta variant by Glory Ranches, Hubert Hackl, Viktoria Zaderer, Melanie Ploner, Wilfried Posch, Doris Wilflingseder, Kai Kummer, Alexander Hüttenhofer

“…In primary human bronchial epithelial cells, we investigated changes in the small RNA transcriptome induced by Delta variant infection. Thereby, we uncovered differential expression of a specific set of microRNAs (miRNAs), PIWI-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), vault RNAs, Y RNAs, and long intergenic non-coding RNAs (lincRNAs), which inhibit apoptosis while promoting cell proliferation and viral infection. …”
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Spatiotemporal dynamic changes of meningeal microenvironment influence meningeal lymphatic function following subarachnoid hemorrhage: from inflammatory response to tissue remodeli... by Bingrui Zhu, Changming Liu, Ming Luo, Jiarui Chen, Sixuan Tian, Tiantong Zhan, Yibo Liu, Haocheng Zhang, Zhen Wang, Jianmin Zhang, Yuanjian Fang, Sheng Chen, Xiaoyu Wang

“…Methods We employed bioinformatics analysis of single-cell RNA sequencing and spatial transcriptomics to characterize the spatiotemporal dynamic changes in the early meningeal microenvironment post-SAH. …”
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Exosomes originating from neural stem cells undergoing necroptosis participate in cellular communication by inducing TSC2 upregulation of recipient cells following spinal cord inju... by Shiming Li, Jianfeng Li, Guoliang Chen, Tao Lin, Penghui Zhang, Kuileung Tong, Ningning Chen, Shaoyu Liu

“…We previously demonstrated that inhibiting neural stem cells necroptosis enhances functional recovery after spinal cord injury. While exosomes are recognized as playing a pivotal role in neural stem cells exocrine function, their precise function in spinal cord injury remains unclear. …”
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Recent advances in biomarker detection of oral squamous cell carcinoma by Lan Liu, Xiaowu Zhong, Yue Zhong, Lihua Li

“…This work aims to comprehensively investigate and evaluate the prospective applications and future developmental potential of these technologies while offering an extensive examination of oral squamous cell cancer biomarker research…”
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Unveiling the role of TCF19 in intervertebral disc degeneration with single-cell and bulk RNA sequencing by Xianwei He, Liwei Wu, Hao Zhou

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Genetic predisposition to immune dysregulation and extracellular matrix remodeling in cardiac arrhythmia reveals potential mediation by SPP1+ macrophages by Jie-Yuan Jin, Jie-Yuan Jin, Shuai Guo, Yao Deng, Ya-Qin Chen, Chen Liang, Yu-Jie Jiang, Wang Zhao, Rong Xiang

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Interpretable machine learning-guided single-cell mapping deciphers multi-lineage pancreatic dysregulation in type 2 diabetes by Xueqin Xie, Changchun Wu, Yuhe Yang, Wei Su, Fuying Dao, Jian Huang, Zheng Shi, Hao Lyu, Hao Lin

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Impact of <i>Ectropis grisescens</i> Warren (Lepidoptera: Geometridae) Infestation on the Tea Plant Rhizosphere Microbiome and Its Potential for Enhanced Biocontrol and Plant Healt... by He Liu, Wei Chen, Xiaohong Fang, Dongliang Li, Yulin Xiong, Wei Xie, Qiulian Chen, Yingying You, Chenchen Lin, Zhong Wang, Jizhou Wang, Danni Chen, Yanyan Li, Pumo Cai, Chuanpeng Nie, Yongcong Hong

“…These samples were analyzed using transcriptomic and high-throughput sequencing of 16S rRNA techniques. …”
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Inflammatory factor CCL2 enhances the interaction between monocyte-macrophage cells and liver parenchymal cells to promote liver inflammation and fibrosis in biliary atresia by Xin Li, Shaowen Liu, Tengfei Li, Qianhui Yang, Yuqiang Chen, Yu Meng, Zhiru Wang, Yilin Zhao, Shujian Zhang, Liang Ge, Alimujiang Abudureyimu, Jianghua Zhan

“…Single-cell RNA sequencing (scRNA-seq), spatial transcriptomics (ST), and Mendelian randomization (MR) were integrated for analysis. …”
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Human Extravillous Trophoblasts Require SRC-2 for Sustained Viability, Migration, and Invasion by Vineet K. Maurya, Pooja Popli, Bryan C. Nikolai, David M. Lonard, Ramakrishna Kommagani, Bert W. O’Malley, John P. Lydon

“…Sustained viability, migration, and invasion are essential cellular properties for embryonic extravillous trophoblasts to execute their roles in placental development and function, while derailment of these cellular processes is linked to placental disorders. …”
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DRomics, a workflow to exploit dose-response omics data in ecotoxicology by Delignette-Muller, Marie Laure, Siberchicot, Aurélie, Larras, Floriane, Billoir, Elise

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Cell-specific transcriptional signatures of vascular cells in Alzheimer’s disease: perspectives, pathways, and therapeutic directions by Soumilee Chaudhuri, Minyoung Cho, Julia C. Stumpff, Paula J. Bice, Özkan İş, Nilüfer Ertekin-Taner, Andrew J. Saykin, Kwangsik Nho

“…Recent advances in high throughput single-cell transcriptomics technology have enabled the study of the human brain vasculature at an unprecedented depth. …”
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Physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in Harbin(哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选)... by 王琪瑶(WANG Qiyao), 李丹青(LI Danqing), 郭俊宏(GUO Junhong), 陈晓璇(CHEN Xiaoxuan), 陈炀(CHEN Yang), 夏宜平(XIA Yiping), 张佳平(ZHANG Jiaping), 刘志洋(LIU Zhiyang)

“…We investigated the physiological and molecular responses of their rhizomes to cold stress under freezing conditions through physiological observations, transcriptome sequencing, and gene expression analysis. …”
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Antiaging Effect of 2-<i>O</i>-β-D-Glucopyranosyl Ascorbic Acid Derived from <i>Lycium barbarum</i> L. Through Modulating the IIS Pathway and Gut Microbiota in <i>Caenorhabditis el... by Jiayue Fang, Wei Dong, Jingqian Zheng, Boxuan Han, Yuying Zhang, Jianing Wang, Xiaoxiong Zeng

“…It significantly extended their lifespan, enhanced stress resistance, reduced lipofuscin accumulation, and improved their healthspan, while strengthening antioxidant defenses. Transcriptomic analysis identified the insulin/insulin-like growth factor (IGF)-1 signaling pathway as a key regulator, with quantitative real-time polymerase chain reaction confirming the upregulation of longevity-associated genes. …”
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Integrative multi-omics analysis to gain new insights into COVID-19 by Setegn Eshetie, Karmel W. Choi, Elina Hyppönen, Beben Benyamin, S. Hong Lee

“…Abstract Multidimensional host and viral factors determine the clinical course of COVID-19. While the virology of the disease is well studied, investigating host-related factors, including genome, transcriptome, metabolome, and exposome, can provide valuable insights into the underlying pathophysiology. …”
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Comprehensive Analysis of the Influence of Technical and Biological Variations on De Novo Assembly of RNA-Seq Datasets by Gonzalez Sergio Alberto, Rivarola Maximo, Ribone Andres, Lew Sergio, Paniego Norma

“…While methods and algorithms for transcriptome assembly are continually being developed and published, the quality of de novo assemblies using short reads depends on the complexity of the transcriptome and is limited by several types of errors. …”
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Distinct immune responses associated with vaccination status and protection outcomes after malaria challenge. by Damian A Oyong, Fergal J Duffy, Maxwell L Neal, Ying Du, Jason Carnes, Katharine V Schwedhelm, Nina Hertoghs, Seong-Hwan Jun, Helen Miller, John D Aitchison, Stephen C De Rosa, Evan W Newell, M Juliana McElrath, Suzanne M McDermott, Kenneth D Stuart

“…In-depth single-cell profiling of cell subsets that respond to CHMI in mock-vaccinated individuals showed a predominantly inflammatory transcriptome response. Whole blood transcriptome analysis revealed that gene sets associated with type I and II interferon and NK cell responses were increased in prior to CHMI while T and B cell signatures were decreased as early as one day following CHMI in protected vaccinees. …”
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A Survey of the ATP-Binding Cassette (ABC) Gene Superfamily in the Salmon Louse (Lepeophtheirus salmonis). by Greta Carmona-Antoñanzas, Stephen N Carmichael, Jan Heumann, John B Taggart, Karim Gharbi, James E Bron, Michaël Bekaert, Armin Sturm

“…Searches of both genome and transcriptome allowed the identification of a total of 33 genes / transcripts coding for ABC proteins, of which 3 were represented only in the genome and 4 only in the transcriptome. …”
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The parental non-equivalence of imprinting control regions during mammalian development and evolution. by Reiner Schulz, Charlotte Proudhon, Timothy H Bestor, Kathryn Woodfine, Chyuan-Sheng Lin, Shau-Ping Lin, Marine Prissette, Rebecca J Oakey, Déborah Bourc'his

“…Paternal and maternal imprints have a similar quantitative impact on the embryonic transcriptome. However, transcriptional effects of maternal ICRs are qualitatively focused on the fetal-maternal interface, while paternal ICRs weakly affect non-convergent biological processes, with little consequence for viability at 8.5dpc. …”
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