Molecular characterization and functional analysis of CYP315 A1 in ovarian development in Macrobrachium nipponense by Sufei Jiang, Wenyi Zhang, Jufeng Jiang, Mengying Zhang, Jisheng Wang, Yiwei Xiong, Hui Qiao, Hongtuo Fu

“…Expression in mature ovaries increased sharply (P < 0.01), while it was relatively low in other stages (P < 0.05). …”
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Perilla frutescens Leaf-Derived Extracellular Vesicle-Like Particles Carry Pab-miR-396a-5p to Alleviate Psoriasis by Modulating IL-17 Signaling by Yali Liu, Shanmin Tao, Zhengwei Zhang, Tianjiao Li, Haoran Wang, Jiankang Mu, Yunke Wu, Ziheng He, Cheng Zhang, Dominique Jasmin Lunter, Peng Cao

“…This effect was attributed to reduced oxidative stress, modulation of Treg cells, and promotion of keratinocyte apoptosis. Transcriptomic analysis revealed enrichment of the interleukin-17 (IL-17) signaling pathway, a major driver of psoriasis, while small RNA sequencing identified pab-miR396a-5p, an endogenous microRNA (miRNA) within PLEVPs, as a key regulator. …”
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Tumor microenvironment assessment-based signatures for predicting response to immunotherapy in non-small cell lung cancer by Jiani Wu, Yuanyuan Wang, Zhenhua Huang, Jingjing Wu, Huiying Sun, Rui Zhou, Wenjun Qiu, Zilan Ye, Yiran Fang, Xiatong Huang, Jianhua Wu, Jianping Bin, Yulin Liao, Min Shi, Jiguang Wang, Wangjun Liao, Dongqiang Zeng

“…The IKCscore exhibits promise as a bioindicator that can predict the efficacy of both immunotherapy and immunotherapy-based combination therapies, while providing guidance for personalized therapeutic strategies for advanced NSCLC patients.…”
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Micheliolide Alleviates Hepatic Fibrosis by Inhibiting Autophagy in Hepatic Stellate Cells via the TrxR1/2-Mediated ROS/MEK/ERK Pathway by Yi Liu, Ling Yao, Yuanyuan Liu, Yunheng Yang, Ailing Liang, Honglin He, Yao Lei, Wenfu Cao, Zhiwei Chen

“…Additionally, MCL significantly inhibited LX-2 cell activation by suppressing cell proliferation, extracellular matrix (ECM) production, and autophagy, while activating the MEK/ERK pathway. Moreover, MCL elevated intracellular and mitochondrial reactive oxygen species (ROS) levels, reduced mitochondrial membrane potential, and altered mitochondrial morphology. …”
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Gut microbiota-derived indoleacetic acid attenuates neuroinflammation and neurodegeneration in glaucoma through ahr/rage pathway by Ning Wang, Chengyang Sun, Yijie Yang, Dandan Zhang, Lulu Huang, Chenrui Xu, Minghan Wang, Mengmeng Xu, Tongtong Yan, Yue Wu, Li Xu, Yahan Ju, Hao Sun, Wenyi Guo

“…Abstract Background Gut microbiota has emerged as a promising therapeutic target for neurodegenerative disorders through regulation of neuroinflammatory responses, while its role in optic nerve degeneration remains incompletely characterized. …”
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Fibrocartilage repair involves chronic cellular senescence in a rat model of bone marrow stimulation by Luke Childress, Landon B. Gatrell, Hong Wu, Elisabeth Ferreira, Intawat Nookaew, Ryan M. Porter

“…Conclusions: Senescent cells, including neochondrocytes, are abundant within inferior repair tissue following marrow stimulation. While their fibrogenic SASP is associated with poor healing, their removal after senescence induction does not improve repair tissue quality, possibly due to the lack of a replacement chondrogenic population.…”
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The Role of L-Sorbose Metabolism in Enhancing Fitness and Virulence in Escherichia coli Under Acidic Conditions by Qiu T, Chen X, Deng X, Zhang G, Wen S, Wu J, Sun D, Li T, Yan B, Dai M, Zhong LL, Tian GB

“…A ΔsorD mutant, with deletion of a key gene in L-Sorbose catabolism, was generated, and its growth, viability, virulence factors, proton motive force (PMF), oxidative stress levels, and transcriptomic profiles under acidic pH (3.5– 5.5) were assessed. …”
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GPD2 inhibition impairs coagulation function via ROS/NF-κB/P2Y12 pathway by Jiajie Chen, Guifeng Xu, Zhipeng Xie, Shaoxia Xie, Wenwei Luo, Shilong Zhong, Weihua Lai

“…However, the therapeutic efficacy of ticagrelor is compromised by interindividual variability in bleeding risk. Notably, while inherited genetic variations account for part of this heterogeneity, the dynamic regulatory role of modifiable epigenetic mechanisms—particularly DNA methylation in mediating platelet reactivity—remains inadequately characterized, presenting a critical knowledge gap in optimizing precision antiplatelet strategies. …”
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Balancing LncRNA H19 and miR‐675 Bioconversion as a Key Regulator of Embryonic Myogenesis Under Maternal Obesity by Yao Gao, Md Nazmul Hossain, Liang Zhao, Xiangdong Liu, Yanting Chen, Jeanene Marie Deavila, Mei‐Jun Zhu, Gordon K. Murdoch, Min Du

“…Methods Single‐cell transcriptomic sequencing and GeoMx spatial RNA sequencing were performed to identify the differentially expressed genes between embryos from MO and control (CT) mice. …”
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BRAF V600E mutation associated with papillary high-grade serous ovarian cystadenocarcinoma in a 22q11.2DS patient by Maria Antonietta Castaldi, Nadia Petrillo, Carmine Selleri, Monica Ianniello, Anna Maria Della Corte, Eloisa Evangelista, Luigia De Falco, Roberto Sirica, Marika Casillo, Alessia Caleo, Alessandro Caputo, Pio Zeppa, Salvatore Giovanni Castaldi, Pasqualina Scala, Bianca Serio, Giovanni Savarese, Valentina Giudice

“…Chromosome 22 abnormalities, including del(22q) and duplications, are genetic multisystemic disorders, most commonly affecting cardiovascular, immune, and gastrointestinal systems, while tumors are rarely reported. Methods: In this case report, we described a patient with 22q11.2 deletion syndrome who developed a papillary high-grade serous ovarian cystadenocarcinoma, and targeted- and whole-exome sequencing for genomic alterations identification and RNA-sequencing for transcriptomics analysis were performed. …”
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Cold storage of human precision-cut lung slices in TiProtec preserves cellular composition and transcriptional responses and enables on-demand mechanistic studies by M. Camila Melo-Narvaez, Fee Gölitz, Eshita Jain, Janine Gote-Schniering, Mircea Gabriel Stoleriu, Wilhelm Bertrams, Bernd Schmeck, Ali Önder Yildirim, Ursula Rauen, Timo Wille, Mareike Lehmann

“…Moreover, TiProtec downregulated pathways associated with cell death, inflammation, and hypoxia while activating pathways protective against oxidative stress. …”
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Whole-Genome Resequencing Analysis of Copy Number Variations Associated with Athletic Performance in Grassland-Thoroughbred by Wenqi Ding, Wendian Gong, Tugeqin Bou, Lin Shi, Yanan Lin, Xiaoyuan Shi, Zheng Li, Huize Wu, Manglai Dugarjaviin, Dongyi Bai

“…After merging overlapping CNVs, 982 copy number variation regions (CNVRs) were recognized, among which the racing horse group (RH) had 29 unique CNVRs, while the non-racing horse group (NR) had 4 unique CNVRs. …”
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Genome-wide identification and functional characterization of LBD gene family in four Cymbidium species (Orchidaceae) and potential regulatory role of CsiLBD27 in floral developmen... by Yonglu Wei, Zengyu Lin, Jianpeng Jin, Wei Zhu, Jie Gao, Jie Li, Qi Xie, Chuqiao Lu, Genfa Zhu, Fengxi Yang

“…In Cymbidium ensifolium, 16 CenLBD genes were differentially expressed, while in Cymbidium mannii, 9 CmaLBD gene expression exist obvious circadian rhythm. …”
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Effect of broccoli extract supplement on carcass traits and lipid metabolism in Holstein steers by Shuangshuang Chen, Xinyu Zhang, Tao Zhu, Die Tang, Mingxing Wen, Chuang Tang, Ling Hou, Zhiyao Zeng, Shanfeng Tong, Xuelong Li, Lu Lu, Keren Long, Quanhui Peng, Anan Jiang, Jideng Ma

“…However, broccoli extract supplementation significantly improved feed intake, lying time, rumination rate, and net meat yield, while reducing subcutaneous fat percentage (P &lt; 0.05). 16S rRNA sequencing revealed increased rumen microbial diversity in the 18 g group. …”
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Cellular hierarchy framework based on single-cell and bulk RNA sequencing reveals fatty acid metabolic biomarker MYDGF as a therapeutic target for ccRCC by Ning Wang, Ziyu Xu, Lina Zhang, Lina Zhang, Yanfang Lu, Yanfang Lu, Yanliang Wang, Yanliang Wang, Lei Yan, Lei Yan, Huixia Cao, Huixia Cao, Limeng Wang, Limeng Wang, Fengmin Shao, Fengmin Shao

“…Nevertheless, the effect of FAM reprogramming on the heterogeneity and prognosis of ccRCC individuals remains insufficiently understood.MethodsWe utilized single-cell sequencing and spatial transcriptomics to investigate the heterogeneity of FAM in ccRCC comprehensively. …”
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Identification of GiOMT gene family in Glycyrrhiza inflata bat and expression analysis under UV-B stresses by Caijuan Li, Hao Li, Yufan Liang, Rong Miao, Ziliang Liu, Sijin Chen, Dan Zhang, Cuiling Wang, Jiling Wu, Xiangyan Zhou, Mengfei Li

“…Phylogenetic relationships showed that the OMT genes of three species, G. inflata, Arabidopsis and alfalfa, were distributed in three taxa, while the GiOMT genes were distributed in taxa I and II. …”
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The context-dependent epigenetic and organogenesis programs determine 3D vs. 2D cellular fitness of MYC-driven murine liver cancer cells by Jie Fang, Shivendra Singh, Brennan Wells, Qiong Wu, Hongjian Jin, Laura J Janke, Shibiao Wan, Jacob A Steele, Jon P Connelly, Andrew J Murphy, Ruoning Wang, Andrew Davidoff, Margaret Ashcroft, Shondra M Pruett-Miller, Jun Yang

“…3D cellular-specific epigenetic and transcriptomic reprogramming is critical to organogenesis and tumorigenesis. …”
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A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming by Ziying Wu, Xiewei Lin, Yao Ying, Gaowei Fan, Junyao Shi, Xiaoqing Zheng, Ben Hu, Hungchen Che, Huiyang Chen, Weilong Yang, Xindi Fan, Ke Mo, Junming Wu, Zhien Lan, Zhiqiang Yu, Shengtao Wang, Chunhui Cui

“…Here, we utilized single‐cell transcriptomics of clinical CRC samples identified NOX4+ (NADPH oxidase 4 positive) cancer‐associated fibroblasts (CAFs) and CXCR4+ (C-X-C motif chemokine receptor 4 positive)/GPX4+ (glutathione peroxidase 4 positive) tumor cells as critical drivers of metastasis. …”
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Cadmium induced ferroptosis and inflammation in sheep via targeting ACSL4/NF-κB axis by Zimeng Ma, Zimeng Ma, Shuo Yan, Shuo Yan, Huimin Zhang, Huimin Zhang, Ruilin Du, Ruilin Du, Xinyue Cheng, Xinyue Cheng, Siqin Bao, Siqin Bao, Xihe Li, Xihe Li, Xihe Li, Yongli Song, Yongli Song

“…Notably, sodium octanoate exhibited potent anti-inflammatory effects through specific binding to ACSL4, a critical ferroptosis regulatory protein, this interaction ameliorated oxidative stress and inflammation cascades while demonstrating therapeutic potential for cadmium-induced inflammation.DiscussionOur findings establish the ACSL4/NF-κB axis as a central mechanism in cadmium-induced pathology, highlighting sodium octanoate as a potential therapeutic intervention for pollutant-induced intestinal disorders.…”
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Single‐cell dissection reveals immunosuppressive F13A1+ macrophage as a hallmark for multiple primary lung cancers by Chenglin Yang, Jiahao Qu, Jingting Wu, Songhua Cai, Wenyi Liu, Youjun Deng, Yiran Meng, Liuqing Zheng, Lishen Zhang, Li Wang, Xiaotong Guo

“…Conclusions This study unveils shared molecular mechanismsbetween MPLCs and SPLCs, while identifying MPLC‐specific cellular and molecularfeatures, such as the role of F13A1+ macrophages. …”
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