Bioprinted Patient‐Derived Organoid Arrays Capture Intrinsic and Extrinsic Tumor Features for Advanced Personalized Medicine by Jonghyeuk Han, Hye‐Jin Jeong, Jeonghan Choi, Hyeonseo Kim, Taejoon Kwon, Kyungjae Myung, Kyemyung Park, Jung In Park, Samuel Sánchez, Deok‐Beom Jung, Chang Sik Yu, In Ho Song, Jin‐Hyung Shim, Seung‐Jae Myung, Hyun‐Wook Kang, Tae‐Eun Park

“…This method achieves an uniform size and shape within PDOs from the same patient while preserving distinct morphological features among those from different individuals. …”
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Pulsed electromagnetic stimulation promotes neuronal maturation by up-regulating cholesterol biosynthesis by Ping Chen, Jingyi Li, Vsevolod Telezhkin, Yu Gu, Min Tao, Liping Guo, Simin Song, Rihe Dong, Xianyang Luo, Yan Wang, Qian Liu, Weiming Tian, Weihua Meng, Wei Hong, Bing Song

“…However, neurons derived from transplanted stem cells often exhibit low survival rates and remain in an immature state. While pulsed electromagnetic fields (PEMF) may enhance neuronal differentiation, the extent of this effect and its molecular mechanisms remain poorly characterized. …”
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The ERBB2 c.1795C>T, p.Arg599Cys variant is associated with left ventricular outflow tract obstruction defects in humans by Minna Ampuja, Sabina Ericsson, Ilkka Paatero, Iftekhar Chowdhury, Jenna Villman, Martin Broberg, Amanda Ramste, Diego Balboa, Tiina Ojala, Jessica X. Chong, Michael J. Bamshad, James R. Priest, Markku Varjosalo, Riikka Kivelä, Emmi Helle

“…Proband-derived human induced pluripotent stem cell cardiomyocytes (hiPS-CMs) and endothelial cells (hiPS-ECs) were used for transcriptomic analyses. While phosphorylation of the ERBB2 p.Arg599Cys receptor was not altered, the variant affected dramatically the binding partners of the protein, indicating mislocalization of the mutant ERBB2 from plasma membrane to endoplasmic reticulum. …”
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Early precursor-derived pituitary gland tissue-resident macrophages play a pivotal role in modulating hormonal balance by Henna Lehtonen, Heli Jokela, Julian Hofmann, Lauriina Tola, Arfa Mehmood, Florent Ginhoux, Burkhard Becher, Melanie Greter, Gennady G. Yegutkin, Marko Salmi, Heidi Gerke, Pia Rantakari

“…Microglia-like macrophages (ml-MACs) are enriched in the posterior pituitary, while other pitMØs in the anterior pituitary exhibit close interactions with hormone-secreting cells. …”
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Improved VPS4B O-GlcNAc modification triggers lipid droplets transferring from adipocytes to nasopharyngeal carcinoma cells by Haimeng Yin, Ying Shan, Qin Zhu, Ling Yuan, Feng Ju, Yu Shi, Yumo Han, Rui Wu, Tian Xia, Kaiwen Zhang, Yiwen You, Bo You

“…NPC cells internalize LDs directly via macropinocytosis, while H-exo induces oxidative stress and membrane fluidity in adipocytes, leading to LD release. …”
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Role of riboflavin biosynthesis gene duplication and transporter in Aeromonas salmonicida virulence in marine teleost fish by Hajarooba Gnanagobal, Trung Cao, Ahmed Hossain, Ignacio Vasquez, Setu Chakraborty, Joy Chukwu-Osazuwa, Danny Boyce, María Jesus Espinoza, Víctor Antonio García-Angulo, Javier Santander

“…Monocistronic mRNA ribA, ribB and ribE2 encode for their corresponding functional riboflavin biosynthetic enzyme. While the product of ribBA conserved the RibB function, it lacked the RibA function. …”
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Unraveling the Heterogeneity of CD8+ T-Cell Subsets in Liver Cirrhosis: Implications for Disease Progression by Kepu Zheng, Leiyang Dai, Shengning Zhang, Yingpeng Zhao, Wang Li, Yang Gao, Yuanyi Mang, Lingfeng Jiao, Yu Tang, Jianghua Ran

“…Methods: This study analyzed transcriptomic and single-cell sequencing data to elucidate CD8+ T-cell heterogeneity and implications in cirrhosis. …”
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Integrated single cell and bulk RNA sequencing analyses reveal the impact of tryptophan metabolism on prognosis and immunotherapy in colon cancer by Yanyan Hu, Ximo Xu, Hao Zhong, Chengshen Ding, Sen zhang, Wei Qin, Enkui Zhang, Duohuo Shu, Mengqin Yu, Naijipu Abuduaini, Xiao Yang, Bo Feng, Jianwen Li

“…Transcriptomic sequencing findings were corroborated by single-cell sequencing from Shanghai Ruijin Hospital. …”
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Enhancing Monacolin K and GABA Biosynthesis in <i>Monascus pilosus</i> via <i>GAD</i> Overexpression: Multi-Omics Elucidation of Regulatory Mechanisms by Wenlan Mo, Yiyang Cai, Simei Huang, Lishi Xiao, Yanfang Ye, Bin Yang, Chan Zhang, Zhiwei Huang

“…Among the 20 PCR-positive transformants obtained, 13 strains exhibited MK production increases of 12.84–52.50% compared to the parental strain, while 17 strains showed GABA production increases of 17.47–134.14%. …”
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Characterization of subcutaneous and visceral de-differentiated fat cells by Yan Li, Houyu Zhang, Carlos F. Ibáñez, Meng Xie

“…We also show that high-fat diet (HFD) feeding enhances DFAT cell colony formation and re-differentiation into adipocytes, while switching from HFD to chow diet (CD) only reverses their re-differentiation. …”
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Salidroside can protect against ferroptosis in cardiomyocytes and may be related to the regulation of GGT1 by Tianhang Feng, Jing Shi, Jinghua Zhao, Qin Zhao, Tao Wang, Sha Wan, Chen Fan, Sijia Wang, Chunyou Lai, Yutong Yao

“…In MI mice, SAL downregulated GGT1 expression while restoring ferroptosis-related biomarkers: upregulating GPX4 and reducing SLC7A11/LC3II levels. …”
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Transient pharmacological inhibition of SUMOylation during pregnancy induces craniofacial malformations in offspring mice by Jorge Mata-Garrido, Isabella Zafferri, Alice Nordlinger, Yann Loe-Mie, Anne Dejean, Jack-Christophe Cossec

“…Post-translational modification by the ubiquitin-related SUMO protein acts as a chromatin barrier to cell fate conversions. While SUMOylation deficiency is incompatible with mammalian embryonic development, haploinsufficiency for the SUMOylation machinery's E1 enzyme, UBA2, leads to various phenotypic traits in humans, including craniofacial malformations and aplasia cutis congenita. …”
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Engineering and application of multiepitope recombinant proteins to enhance resistance to Botrytis cinerea in tomatoes: a new paradigm for creating plant immune activators by Xiaxia Man, Huang You, Zhiqiang Cheng, Junhao Li, Dunchao Yao, Haofeng Wang, Zhihong Diao, Xiaosong Yu, Wei Wu, Cheng Zhou, Yan Huang, Jinbo Shen, Xiaohong Zhuang, Yi Cai

“…In further in vivo experiments, SlRP5 alleviated B. cinerea-induced membrane damage by reducing MDA and REC levels, while simultaneously enhancing the activities of antioxidant enzymes such as SOD, CAT, and POD, thereby mitigating the excess ROS generated by infection. …”
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Alanine metabolism mediates energy allocation of the brown planthopper to adapt to resistant rice by Kai Lin, Lei Yue, Longyu Yuan, Kui Kang, Yibing Zhang, Rui Pang, Wenqing Zhang

“…Introduction: During the adaptation to host plant resistance, herbivorous insects faced the challenge of overcoming plant defenses while ensuring their own development and reproductive success. …”
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SENP1-Sirt3 axis regulates type II alveolar epithelial cell activity to confer resistance against oxidative damage in lung tissue by Mingming Zhang, Xin Lin, Jianli He, Yong Zuo, Qiuju Fan, Innocent Agida, Hongsheng Tan, Caiying Zhu, Jinke Cheng, Tianshi Wang

“…This intervention increased AT2 cell proliferation and differentiation into alveolar type I cells while reducing Keratin 8 (KRT8)+ transitional cell number, a profibrotic population. …”
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Intraindividual epigenetic heterogeneity underlying phenotypic subtypes of advanced prostate cancer by Kei Mizuno, Sheng-Yu Ku, Varadha Balaji Venkadakrishnan, Martin K. Bakht, Michael Sigouros, Joanna Chan, Anna Trigos, Jordan H. Driskill, Jyothi Manohar, Abigail King, Adam G. Presser, Min Jin Kim, Alok K. Tewari, Henry W. Long, David Quigley, Toni K. Choueiri, Steven Balk, Sarah Hill, Juan Miguel Mosquera, David Einstein, Shahneen Sandhu, Mary-Ellen Taplin, Himisha Beltran

“…These include cases that retain expression of luminal markers and those that lose hormone dependence and acquire neuroendocrine features. While there are distinct transcriptomic and epigenomic differences between castration-resistant adenocarcinoma and neuroendocrine prostate cancer, the extent of overlap and degree of diversity across tumor metastases in individual patients has not been fully characterized. …”
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Identification and phylogenetic analysis of Nairobi sheep disease virus from Haemaphysalis longicornis ticks in Shandong Province, China by Yunxiao Wang, Ruiling Zhang, Xiurong Wang, Xudong Zhang, Zhong Zhang, Michael J. Carr, Guangfu Yu, Hong Zhou, Weifeng Shi

“…The SDWL07 and SDWL16 strains were detected from unfed ticks, while SDWL08 was detected from cattle-feeding ticks. …”
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A genome-wide association study in 10,000 individuals links plasma N-glycome to liver disease and anti-inflammatory proteins by Sodbo Sharapov, Anna Timoshchuk, Olga Zaytseva, Denis E. Maslov, Anna Soplenkova, Elizaveta E. Elgaeva, Evgeny S. Tiys, Massimo Mangino, Clemens Wittenbecher, Lennart Karssen, Maria Timofeeva, Arina Nostaeva, Frano Vuckovic, Irena Trbojević-Akmačić, Tamara Štambuk, Sofya Feoktistova, Nadezhda A. Potapova, Viktoria Voroshilova, Frances Williams, Dragan Primorac, Jan Van Zundert, Michel Georges, Karsten Suhre, Massimo Allegri, Nishi Chaturvedi, Malcolm Dunlop, Matthias B. Schulze, Tim Spector, Yakov A. Tsepilov, Gordan Lauc, Yurii S. Aulchenko

“…Most of them are synthesized, glycosylated, and secreted to the bloodstream by liver and lymphoid tissues. While associations with N-glycosylation are implicated in the rising number of liver, cardiometabolic, and immune diseases, little is known about the genetic regulation of this process. …”
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Effect of immune infiltration intensity on the efficacy of neoadjuvant immunotherapy for esophageal cancer by Yong Zhang, Xinyao Xu, Xinyao Xu, Xiaorong Mu, Xiaorong Mu, Juzheng Wang, Jipeng Zhang, Guangyu Xiang, Jiahe Li, Chunlong Zheng, Huaiyu Wang, Qiang Lu

“…However, the effectiveness of these treatments is limited by immune infiltration in the tumor microenvironment.MethodsWe analyzed single-cell transcriptomic data from 22 patients with resectable ESCC, collected before and after neoadjuvant therapy. …”
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Soluble TIM-3, likely produced by myeloid cells, predicts resistance to immune checkpoint inhibitors in metastatic clear cell renal cell carcinoma by Ivan Pourmir, Nadine Benhamouda, Thi Tran, Hugo Roux, Joséphine Pineau, Alain Gey, Andyara Munoz, Nesrine Mabrouk, Nicolas Epaillard, Virginie Verkarre, Yann-Alexandre Vano, Eric Tartour, Stéphane Oudard

“…It shows distinct associations with survival on anti-PD-1 vs anti-PD-1 + anti-CTLA-4: under nivolumab monotherapy, sTIM-3-high patients have a significantly reduced survival compared to sTIM-3-low patients, while they have similar survival probabilities under N + I. sTIM-3 is independent from other clinical and biological factors. …”
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