Orf132: a critical gene for LSDV replication and its role in Er stress-related apoptosis by Yongtao Wang, Shiwei Zhang, Hailing Li, Yali Feng, Ying Zhang

“…It plays an essential role in the virus’s life cycle, and its deletion significantly impairs viral replication while inducing ER stress-related apoptosis.…”
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Development of a four autophagy-related gene signature for active tuberculosis diagnosis by Baoyan Ren, Feng Jia, Qixun Fang, Jingping Xu, Jingping Xu, Kangfeng Lin, Kangfeng Lin, Renhui Huang, Zhenqiong Liu, Xingan Xing, Xingan Xing

“…RT-qPCR validation in whole blood samples confirmed elevated expression in ATB, while an SVM model achieved promising differentiation (AUC 0.86 for ATB vs. …”
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Heme oxygenase 1 confers gilteritinib resistance in FLT3-ITD acute myeloid leukemia in a STAT6-dependent manner by Tianzhuo Zhang, Danna Wei, Yun Zhan, Zhengmei Long, Tingting Lu, Peng Zhao, Rui Gao, Qian Kang, Luxin Zhang, Min Liu, Xueying Yang, Jishi Wang

“…Methods RNA sequencing was conducted to assess transcriptomic changes in three pairs of AML cells after regulating the expression of HO1. …”
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Temporal changes in the proanthocyanidins to anthocyanins ratio during dormancy associate with bloom time variations in peach by Protiva Rani Das, Md Tabibul Islam, Jianyang Liu, Zongrang Liu, Chris Dardick, Sherif M. Sherif

“…Late-bloom cultivars had higher PA levels during endodormancy, while early-bloom cultivars had higher ACN levels during ecodormancy. …”
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Omega-3 Fatty Acids Regulate Mammary Gland Lipogenesis and Development via Gαs-Mediated cAMP–EPAC Signaling Pathway by Baofeng Li, Senlin Su, Siyu Yuan, Dongpang Chen, Qianzi Zhang, Qihui Li, Xiaoai Lin, Xiaohuan Liang, Wutai Guan, Shihai Zhang

“…We discovered that the GPR120–Gαs–cyclic adenosine monophosphate (cAMP)–exchange protein directly activated by cAMP (EPAC) signaling axis is critical for lipogenesis and migration in mammary epithelial cells. Through transcriptomic analyses, the EPAC–CCCTC-binding factor (CTCF)–peroxisome proliferator-activated receptor γ (PPARγ)/CCAAT enhancer-binding protein α (C/EBPα) pathway was identified to primarily govern lipogenesis, while the EPAC–C-X-C motif chemokine ligand 14 (CXCL14)/C-X-C chemokine receptor type 4 (CXCR4) autocrine loop regulates migration of mammary epithelial cells. …”
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Integrative bioinformatics and machine learning identify iron metabolism genes MAP4, GPT, and HIRIP3 as diagnostic biomarkers and therapeutic targets in Alzheimer’s disease by Xiaoqiong An, Xiangguang Zeng, Zhenzhen Yi, Manni Cao, Yijia Wang, Wenfeng Yu, Wenfeng Yu, Zhenkui Ren, Zhenkui Ren

“…In APP/PS1 mice, MAP4 decreased, while GPT and HIRIP3 increased.DiscussionThis analysis highlights these genes as diagnostic biomarkers and therapeutic targets, connecting iron balance, neuroinflammation, and metabolic problems in AD. …”
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Ophiopogonin D improves pancreatic islet cell dedifferentiation to treat diabetes by regulating the GRP78/ROS/PDX1 signaling pathway by Haoxiang Sun, Ruixiang Tan, Yongzhi Sun, Yimeng Li, Ying Xie, Cheng Zhang, Jianping Song, Wei Zhu, Jiuyao Zhou, Changsheng Deng, Manxue Mei

“…Furthermore, Op D inhibited ER stress, decreased GRP78 expression, reduced NGN3 levels, elevated PDX1, NKX6.1, and MAFA expression, and decreased oxidative stress-related products (ROS, MDA) while increasing SOD and GSH levels.DiscussionThese findings demonstrate that Op D can improve β-cell dedifferentiation by modulating the GRP78/ROS/PDX1 pathway to inhibit ER stress.…”
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Osteoclast-independent osteocyte dendrite defects in mice bearing the osteogenesis imperfecta-causing Sp7 R342C mutation by Jialiang S. Wang, Katelyn Strauss, Caroline Houghton, Numa Islam, Sung-Hee Yoon, Tatsuya Kobayashi, Daniel J. Brooks, Mary L. Bouxsein, Yingshe Zhao, Cristal S. Yee, Tamara N. Alliston, Marc N. Wein

“…Abstract Osteogenesis imperfecta (OI) is a group of diseases caused by defects in type I collagen processing which result in skeletal fragility. While these disorders have been regarded as defects in osteoblast function, the role of matrix-embedded osteocytes in OI pathogenesis remains largely unknown. …”
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The glutamate receptor gene GLR3.3: A bridge of calcium-mediated root development in poplar by Yi An, Ya Geng, Yu Liu, Xiao Han, Lichao Huang, Wei Zeng, Jin Zhang, Mengzhu Lu

“…In this study, we analyzed the morphological and transcriptomic variations of 84K poplar (Populus alba × P. glandulosa) in response to different calcium concentrations and found that low Ca2+ (1 mmol · L−1) promoted lateral root development, while deficiency (0.1 mmol · L−1 Ca2+) inhibited lateral root development. …”
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Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis by Tingji Yang, Bo Fang, Yan Chen, Dan Bao, Jiang Zhang, Peiwen Liu, Zhiwei Duan, Yuxuan He, Xingxu Zhao, Quan-Wei Zhang, Wei-Tao Dong, Yong Zhang

“…When ARPC3/ARPC4 was inhibited using CK666, HSP70 expression further increased compared to the LPS group, while inflammatory factors, apoptosis rates, and apoptosis-related protein expression were notably reduced. …”
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Neutrophil Myo5c gene downregulation is associated with postoperative organ dysfunction following pediatric cardiac surgery with cardiopulmonary bypass by Wiriya Maisat, Wiriya Maisat, Wiriya Maisat, Wiriya Maisat, Sumiti Sandhu, Samuel Kim, Hanna Van Pelt, Sek Won Kong, Sek Won Kong, Juan Ibla, Juan Ibla, Koichi Yuki, Koichi Yuki, Koichi Yuki, Koichi Yuki

“…As neutrophils play a major role in the perioperative setting and act as double-edge swords to the host, we examined neutrophil transcriptomic profiles in pediatric patients undergoing cardiac surgery with CPB.MethodsWe enrolled into this study from May 31, 2022, to February 22, 2023.Results32% developed postoperative complications, mainly in the respiratory and cardiovascular systems. …”
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Divergent therapeutic and prognostic impacts of immunogenic features in undifferentiated pleomorphic sarcoma and myxofibrosarcoma by Siddh van Oost, Debora M. Meijer, Zeynep B. Erdem, Marieke E. IJsselsteijn, Jessica Roelands, Suk Wai Lam, Melissa S. Boejharat, Brendy E. W. M. van den Akker, Ruud van der Breggen, Inge H. Briare-de Bruijn, Lukas J. A. C. Hawinkels, Anouk A. Kruiswijk, Manon van der Ploeg, Pauline M. Wijers-Koster, Rick L. Haas, Michiel A. J. van den Sande, Noel F. C. C. de Miranda, Judith V. M. G. Bovee

“…UPS and MFS demonstrated immunogenic features compared to other soft tissue sarcomas, with subsets of UPS and MFS demonstrating high T cell infiltration, while UPS demonstrated a higher infiltration by myeloid cells as compared to MFS. …”
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Distinct microRNA signatures define sporadic PSP-RS and PD in patient-derived midbrain organoids by Desirèe Valente, Clara Zannino, Stefania Scalise, Davide Bressan, Valeria Lucchino, Ermes Filomena, Giorgia Lucia Benedetto, Ida Lazzinnaro, Mariagrazia Talarico, Andrea Quattrone, Fulvio Chiacchiera, Graziano Pesole, Aldo Quattrone, Elvira Immacolata Parrotta, Giovanni Cuda

“…Notably, miR-5683, miR-873-5p, miR-219b-5p, and miR-219a-2-3p were enriched in PSP-RS, while PD organoids showed increased expression of miR-1-3p and miR-133b. …”
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Effects of different fiber levels of energy feeds on rumen fermentation and the microbial community structure of grazing sheep by Xiaoyun Zhang, Xulei Liu, Kaili Xie, Yueting Pan, Fuyao Liu, Fujiang Hou

“…Low-fiber-energy feeds significantly increased the relative abundance of Actinobacteria, while the relative abundances of Cyanobacteria, Ruminococcaceae_UCG_010, Ruminococcaceae_NK4A214_group, and Elusimicrobium significantly decreased, adjusting the relationship between the flora toward cooperation. …”
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Prognostic and tumor microenvironmental features of gastric cancer revealed by macrophage polarization and protein lactylation-related genes by Zifan Xu, Zifan Xu, Zi Lei, Shilan Peng, Sha Li, Dehui Kong, Hongqiong Duan, Man Zhang, Guomiao Su, Guoqing Pan

“…ERCC6L is located on chromosome X, while MYB is located on chromosome 6. Gene-gene interaction network analysis revealed interactions between prognostic genes and other key genes. …”
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Integrative genomic and bioinformatic prioritization of drug repurposing candidates for prostate cancer by Lalu Muhammad Irham, Wirawan Adikusuma, Arief Rahman Afief, Sabiah Khairi, Rockie Chong, Syarifatul Mufidah, Rahmat Dani Satria, Eko Mugiyanto, Darmawi Darmawi, Danang Prasetyaning Amukti, Brilliant Citra Wirsahada, Petrina Theda Philothra, Indra Jaya

“…Molecular docking analysis further supported these findings, revealing that estradiol-benzoate and estradiol-cypionate have strong predicted binding affinities for ESR2, while selumetinib robustly interacts with MAP2K1. …”
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Unique N-glycosylation signatures in human iPSC derived microglia activated by Aβ oligomer and lipopolysaccharide by Xinyu Tang, Ryan Lee Schindler, Jacopo Di Lucente, Armin Oloumi, Jennyfer Tena, Danielle Harvey, Carlito B. Lebrilla, Angela M. Zivkovic, Lee-Way Jin, Izumi Maezawa

“…Here, we study comprehensively combined transcriptomic and glycomic profiles using human induced pluripotent stem cells-derived microglia (hiMG). …”
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MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration by Ana Patrícia Graça, Vadim Nikitushkin, Mark Ellerhorst, Cláudia Vilhena, Tilman E Klassert, Andreas Starick, Malte Siemers, Walid K Al-Jammal, Ivan Vilotijevic, Hortense Slevogt, Kai Papenfort, Gerald Lackner

“…Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily, remained functionally uncharacterized. …”
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SHED-derived exosome-mimetics promotes rotator cuff tendon-bone healing via macrophage immunomodulation through NF-κB suppression and autophagy activation by Yanwei He, Junzhe Sheng, Fashun Liu, Fangqi Li, Shihao Lu, Wenbo Chen, Li Yang, Peng Zhou, Zan Chen, Shiyi Chen, Zhiwen Luo, Junming Sun

“…In vivo, GM@PDA&EMs reduced M1 macrophage infiltration, enhanced osteocalcin and VEGF expression, and improved biomechanical strength. Transcriptomic analysis of macrophages revealed SHED-EMs suppressed NF-κB signaling, while subsequent experiments demonstrated autophagy activation (increased LC3II/I, decreased P62) and reduced inflammation (lower TNF-α/iNOS). …”
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UV-B induced expression of wax synthesis gene LbCYP96A15 in leaves of goji plants (Lycium barbarum) to improve powdery mildew resistance by Xiao Zhang, Jie Li, Xia Wen, Xin-bing Wang, Deng-pan Shen, Li-wei Ding, Jing He

“…Concurrently, the incidence of powdery mildew rose from 7.85 to 21.7% and 72.92%, while the disease index increased from 14.21 to 27.23 and 78.40, respectively. …”
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