Multifunctional DNA hydrogels with light-triggered gas-therapy and controlled G-Exos release for infected wound healing by Yuyun Ye, Yan Liu, Shengchao Ma, Xipeng Li, Wei Wang, Xu Chen, Judun Zheng, Zhijin Fan, Yideng Jiang, Yuhui Liao

“…In vivo experiments demonstrated that LGAH significantly accelerates S. aureus-infected wound healing by modulating the wound microenvironment and promoting tissue regeneration. Transcriptomic analysis revealed that LGAH down-regulates gene expression in inflammation and immune response signaling pathways while up-regulating genes related to energy metabolism. …”
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Evaluation of pyroptosis-associated genes in endometrial cancer utilizing a 101-combination machine learning framework and multi-omics data by Li Juan Huang, Chen Liu, Lin Chen, Min Tang, Shi Tong Zhan, Feng Chen, An Yi Teng, Li Na Zhou, Wei Lin Sang, Ye Yang

“…Pyroptosis, a pro-inflammatory form of programmed cell death, plays dual roles in cancer but remains poorly understood in the context of EC and its immune microenvironment.MethodsWe identified pyroptosis-associated genes (PAGs) and applied a 101-combination machine learning framework to construct and validate a robust prognostic model using TCGA bulk RNA-seq and single-cell transcriptomic data. Immune infiltration was assessed using CIBERSORT and Tumor Immune Dysfunction and Exclusion (TIDE), while CellChat was employed to investigate pyroptosis-related cell–cell communication. …”
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The SOX11:SMARCA4 complex is a driver of oncogenic transcriptional programs in mantle cell lymphoma by Anna De Bolòs, Maria Carreras-Caballé, Marta Sureda-Gómez, Marta-Leonor Rodríguez, Stella Charalampopoulou, Robert Albero, Alba Maiques-Diaz, Heribert Playa-Albinyana, Guillem Clot, Cristina López, Dolors Colomer, José I Martin-Subero, Elías Campo, David Reverter, Josep Villanueva, Virginia Amador

“…The transcription factor SOX11 is aberrantly expressed in conventional aggressive MCL, while it is not or weakly expressed in the leukemic non-nodal MCL subtype with a predominantly indolent clinical evolution. …”
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Synergistic effects of ectoine and biostimulants combinations on tomato seedling growth and heat stress resilience by Yanyan Chen, Weimin Xu, Chuankun Han, Miao Zhang, Ying Chen, Yujia Ma, Xinjun Zhang, Huhu Liu, Wenna Zhang

“…Transcriptomic analysis revealed significant differences in differentially expressed genes (DEGs) among treatments. …”
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Microbial genetic variation impacts host eco-immunological strategies and microparasite fitness in Lyme borreliae-reptile system by Tristan A. Nowak, Carly Fernandes, Jill Malfetano, Erica Lasek-Nesselquist, Matthew Combs, Klemen Strle, Russell L. Burke, Yi-Pin Lin

“…In the strategy of “resistance”, host responses are induced to decrease microparasite replication while the “tolerance” strategy allows hosts coexistence with microparasites by minimizing responses to avoid immune-mediated damage. …”
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ApoE2 affects insulin signaling in the hippocampus and spatial cognition of aged mice in a sex-dependent manner by Yu Wang, Hanchen Liu, Yijuan Ye, Wenting Fang, Anlan Lin, Xiaoman Dai, Qinyong Ye, Xiaochun Chen, Jing Zhang

“…Abstract Apolipoprotein E (APOE) has garnered significant attention as one of the most influential genetic risk factors for Alzheimer’s disease (AD). While the pathogenic role of APOE4 in sporadic AD has been extensively studied, research on the protective effects of the APOE2 genotype and its underlying mechanisms remains limited. …”
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Proton motive force generated by microbial rhodopsin promotes extracellular electron transfer by Wenqi Ding, Tong Lin, Yun Yang, Wen-Wei Li, Shaoan Cheng, Hao Song

“…Nevertheless, the impact of PMF on the EET efficiency remains ambiguous, while the microbial rhodopsin offers a simple and efficient avenue for non-photosynthetic cells to harness PMF. …”
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Human cytomegalovirus UL82 promotes cell cycle progression of colorectal cancer by upregulating AGR2 by Haitao Ren, Bing Wang, Lanni Wang, Ye Shi, Ruini Li, Chaoyi Jiang, Jingxin Feng, Jiahao Wang, Hanru Yao, Linhua Lan, Guohui Gao, Liyi Li, Guangxin Xiang, Feng Xu, Xiaoqun Zheng

“…Additionally, UL82 enhanced the expression of the oncogene AGR2, while knockdown of AGR2 abolished the proliferative effect of UL82. …”
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Exploring therapeutic paradigm focusing on genes, proteins, and pathways to combat leprosy and tuberculosis: A network medicine and drug repurposing approach by Mohd Imran, Ahmed S. Alshrari, Mariah N. Hafiz, Mohammed M. Jawad, Abida Khan, Fadiyah Jadid Alanazi, Syed Mohammed Basheeruddin Asdaq

“…Methods: Transcriptomic datasets were analyzed to identify dysregulated genes and pathways. …”
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A multivariate cell-based liquid biopsy for lung nodule risk stratification: Analytical validation and early clinical evaluation by Jason D. Berndt, Fergal J. Duffy, Mark D. D'Ascenzo, Leslie R. Miller, Yijun Qi, G. Adam Whitney, Samuel A. Danziger, Anil Vachani, Pierre P. Massion, Stephen A. Deppen, Robert J. Lipshutz, John D. Aitchison, Jennifer J. Smith

“…In the platform, standardized cells are exposed to small volumes of patient serum, and the resulting transcriptomic response is analyzed using machine learning tools to develop disease classifiers. …”
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The janus face of astrocytes in multiple sclerosis: Balancing protection and pathology by Ahmad A. Abulaban, Hayder M. Al-kuraishy, Ali I. Al-Gareeb, Ali K. Albuhadily, Mustafa M. Shokr, Athanasios Alexiou, Marios Papadakis, Gaber El-Saber Batiha

“…Multiple sclerosis (MS) is a chronic autoimmune disorder characterized by demyelination and neurodegeneration in the central nervous system (CNS), predominantly affecting young adults with a notable female predominance. While the pathogenesis of MS involves complex interactions between peripheral immune cells and CNS glia, astrocytes—the most abundant glial cells—play a dual role in disease progression. …”
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CYP1B1-AS1 regulates CYP1B1 to promote Coxiella burnetii pathogenesis by inhibiting ROS and host cell death by Aryashree Arunima, Seyednami Niyakan, Samantha M. Butler, Sabrina D. Clark, Anna Pinson, Doyoung Kwak, Elizabeth Di Russo Case, Xiaoning Qian, Paul de Figueiredo, Erin J. van Schaik, James E. Samuel

“…Promoter assays confirm their co-regulation via a shared bidirectional promoter, while aryl hydrocarbon receptor (AHR)-lucia luciferase and nuclear translocation assays demonstrate that Cb infection activates AHR, driving their transcription. …”
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Genome wide identification of LcC2DPs gene family in Lotus corniculatus provides insights into regulatory network in response to abiotic stresses by Guangfen Yang, Yujie Liu, Zouxian Gong, Siya Chen, Juanying Wang, Li Song, Shihui Liu

“…NAC and C2H2 are vital for drought response, while bZIP and MYB-related are important for cold response. …”
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Dissecting endothelial cell heterogeneity with new tools by Jing Zhong, Rong-rong Gao, Xin Zhang, Jia-xin Yang, Yang Liu, Jinjin Ma, Qi Chen

“…Intra-organ heterogeneity encompasses arterio-venous and tip-stalk endothelial cell specialization, while inter-organ heterogeneity refers to organ-specific transcriptomic profiles and functions. …”
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Reprogramming Hairy Root Cultures: A Synthetic Biology Framework for Precision Metabolite Biosynthesis by Chang Liu, Naveed Ahmad, Ye Tao, Hamad Hussain, Yue Chang, Abdul Wakeel Umar, Xiuming Liu

“…In addition, by pointing out essential research areas such as optimizing culture conditions, increasing metabolite yields, and scaling up production, this work strengthens the significance of hairy root cultures in meeting the demand for high-value products while ensuring sustainable resource utilization. …”
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Diallyl disulfide attenuates hydrogen peroxide-induced oxidative damage of ovine rumen epithelial cells through the nuclear factor erythroid-2 related factor 2 signaling pathway by Rui Pang, Qingyue Zhang, Yuxin Wang, Xiaona Dong, Li Huang, Yingying Tang, Chunhuan Ren, Yanfeng Xue, Zijun Zhang, Wen Zhu

“…Results showed that DADS remarkably enhanced superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) (P < 0.05) while reducing ROS and malonaldehyde production (P < 0.05) in H2O2-treated RECs. …”
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Age-associated differences in mucosal and systemic host responses to SARS-CoV-2 infection by Jillian H. Hurst, Aditya A. Mohan, Trisha Dalapati, Ian A. George, Jhoanna N. Aquino, Debra J. Lugo, Trevor S. Pfeiffer, Javier Rodriguez, Alexandre T. Rotta, Nicholas A. Turner, Thomas W. Burke, Micah T. McClain, Ricardo Henao, C. Todd DeMarco, Raul Louzao, Thomas N. Denny, Kyle M. Walsh, Zhaohui Xu, Asuncion Mejias, Octavio Ramilo, Christopher W. Woods, Matthew S. Kelly

“…Peripheral blood responses among SARS-CoV-2-infected children and adolescents are dominated by interferon pathways, while upregulation of myeloid activation, inflammatory, and coagulation pathways is observed only in adults. …”
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Blocking CXCR4+ CD4+ T cells reprograms Treg-mediated immunosuppression via modulating the Rho-GTPase/NF-κB signaling axis by Canhui Cao, Miaochun Xu, Ting Peng, Xiaojie Liu, Shitong Lin, Yashi Xu, Tian Chu, Shiyi Liu, Ping Wu, Bai Hu, Wencheng Ding, Li Li, Ding Ma, Peng Wu

“…Abstract Background While clinical trials have shown that CXCR4 antagonists can enhance the efficacy of cancer immunotherapy, the molecular mechanisms by which CXCR4 modulates the tumor microenvironment remain poorly understood. …”
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Molecular and spatial analysis of tertiary lymphoid structures in Sjogren’s syndrome by Saba Nayar, Jason D. Turner, Saba Asam, Eanna Fennell, Matthew Pugh, Serena Colafrancesco, Onorina Berardicurti, Charlotte G. Smith, Joe Flint, Ana Teodosio, Valentina Iannizzotto, David H. Gardner, Joel van Roon, Ilya Korsunsky, Dawn Howdle, Roche Fibroblast Network Consortium, Andreas P. Frei, Kara G. Lassen, Simon J. Bowman, Wan-Fai Ng, Adam P. Croft, Andrew Filer, Benjamin A. Fisher, Christopher D. Buckley, Francesca Barone

“…Abstract Tertiary lymphoid structures play important roles in autoimmune and non-autoimmune conditions. While many of the molecular mechanisms involved in tertiary lymphoid structure formation have been identified, the cellular sources and temporal and spatial relationship remain unknown. …”
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Physiological Responses and Histopathological Changes in Narrow-Clawed Crayfish (<i>Pontastacus leptodactylus</i>) Under Acute Thermal Stress by Xia Zhu, Bin Li, Yuzhen Liu, Shujian Chen, Yangfang Ye, Ronghua Li, Weiwei Song, Changkao Mu, Chunlin Wang, Ce Shi

“…Similarly, the T25 group displayed increased MDA and CAT levels but decreased T-AOC. Comparative transcriptomic analysis demonstrated that differentially expressed genes (DEGs) in the control vs. …”
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