Mechanistic insights into 5-aminolevulinic acid photodynamic therapy for acne vulgaris: targeting lipogenesis via the OLR1-Wnt/β-catenin pathway by Jia Yan, Linglin Zhang, Qingyu Zeng, Yitao Qian, Ke Li, Xiaojing Liu, Yun Wu, Yu Yan, Haiyan Zhang, Szeman Cheung, Jia Liu, Ronald Sroka, Xiuli Wang, Lei Shi

“…Abstract Acne vulgaris, a prevalent chronic inflammatory skin disorder, is often characterized by hyperactive sebaceous glands and excessive sebum production, presenting a significant therapeutic challenge. While 5-aminolevulinic acid photodynamic therapy (ALA-PDT) is clinically effective in treating moderate to severe acne, the molecular mechanisms underlying its therapeutic effects remain largely unexplored. …”
Get full text

Nanoparticle‐Mediated CXCL12–CXCR4 Inhibition Reprograms Macrophages and Suppresses Gastric Carcinoma by Qianqian Cao, Xiaolei Cheng, Rongbin Lv, Dianshui Sun, Jihua Wang, Runjia Fu, Rumei Gong, Yueying Xiao, Qin Liu, Xiaomei Li

“…Flow cytometry and ELISA quantify immune activation markers; while, cell migration and invasion assays evaluate tumor suppression effects. …”
Get full text

Deep spatial sequencing revealing differential immune responses in human hepatocellular carcinoma by Yan-Ping Yu, Caroline Obert, Bao-Guo Ren, Marielle Krivit, Kyle Metcalfe, Jia-Jun Liu, Jia-Jun Liu, Tuval Ben-Yehezkel, Silvia Liu, Silvia Liu, Jian-Hua Luo

“…In this study, we performed ultra-depth (∼1 million reads per spot) sequencing of 6,320 spatial transcriptomes on a case of HCC. Sixteen distinct spatial expression clusters were identified. …”
Get full text

Chidamide and cytarabine synergistically treat acute myeloid leukemia: inhibiting ribosome biogenesis via the MYC-RRP9 pathway by Qing Li, Fangfang Wang, Xinrong Xiang, Lei Zhao, Xuefeng Li, Ying Qu, Jingcao Huang, Yunfan Yang, Yang Dai, Xiao Shuai, Jiazhuo Liu, Jie Huang, Yu Liu, Yu Wu

“…In both in vitro and in vivo models, the drug combination demonstrated significant synergism in combating acute myeloid leukemia. Transcriptomic analysis revealed that the combination treatment notably downregulates the MYC signaling pathway. …”
Get full text

snCED-seq: high-fidelity cryogenic enzymatic dissociation of nuclei for single-nucleus RNA-seq of FFPE tissues by Yunxia Guo, Junjie Ma, Ruicheng Qi, Rongrong Ma, Xiaoying Ma, Jitao Xu, Kaiqiang Ye, Yan Huang, Xi Yang, Jianyou Zhang, Guangzhong Wang, Xiangwei Zhao

“…Abstract Recent advances have shown that single-nucleus RNA sequencing (snRNA-seq) can be applied to formalin-fixed, paraffin-embedded (FFPE) tissues, opening avenues for transcriptomic analysis of archived specimens. Yet, isolating intact nuclei remains difficult due to RNA cross-linking. …”
Get full text

Loss of <i>Myostatin</i> Alters Gut Microbiota and Carbohydrate Metabolism to Influence the Gut–Muscle Axis in Cattle by Chao Hai, Hongyu Gong, Yanan Xu, Xuefei Liu, Chunling Bai, Guanghua Su, Lei Yang, Guangpeng Li

“…These changes correlated with reduced serum glucose and insulin levels while increasing muscle gene expression related to glucose-to-lactose conversion. …”
Get full text

Cross-species single-cell RNA-seq analysis reveals disparate and conserved cardiac and extracardiac inflammatory responses upon heart injury by Eric Cortada, Jun Yao, Yu Xia, Friederike Dündar, Paul Zumbo, Boris Yang, Alfonso Rubio-Navarro, Björn Perder, Miaoyan Qiu, Anthony M. Pettinato, Edwin A. Homan, Lisa Stoll, Doron Betel, Jingli Cao, James C. Lo

“…Adult mice and humans lack the ability to fully recover while adult zebrafish spontaneously regenerate after heart injury. …”
Get full text

Organoid‐Guided Precision Medicine: From Bench to Bedside by Boqiang Tao, Xiaolan Li, Ming Hao, Tian Tian, Yuyang Li, Xiang Li, Chun Yang, Qirong Li, Qiang Feng, Hengzong Zhou, Yicheng Zhao, Dongxu Wang, Weiwei Liu

“…In precision medicine, organoid‐guided multiomics analyses identify actionable biomarkers and resistance mechanisms, while clustered regularly interspaced short palindromic repeats‐based functional screens optimize therapeutic targeting. …”
Get full text

Integrated omics reveals the regulatory role of PKCα in Sertoli cell proliferation and apoptosis through the MAPK/ERK signaling pathway in goose testis by Yupu Song, Qiuyuan Liu, Jingyun Ma, Ichraf Mabrouk, Yuxuan Zhou, Xinyue Li, Guizhen Xue, Xiaoming Ma, Jing Xu, Jingbo Wang, Hongxiao Pan, Guoqing Hua, Heng Cao, Jingtao Hu, Yongfeng Sun

“…This study employed integrative transcriptomic and proteomic analysis methods to identify critical regulators of testicular development in geese across three reproductive periods. …”
Get full text

Cholangiocytes contribute to hepatocyte regeneration after partial liver injury during growth spurt in zebrafish by Sema Elif Eski, Jiarui Mi, Macarena Pozo-Morales, Gabriel Garnik Hovhannisyan, Camille Perazzolo, Rita Manco, Imane Ez-Zammoury, Dev Barbhaya, Anne Lefort, Frédérick Libert, Federico Marini, Esteban N. Gurzov, Olov Andersson, Sumeet Pal Singh

“…Minor injuries are repaired by hepatocyte self-duplication, while severe damage triggers cholangiocyte involvement in hepatocyte recovery. …”
Get full text

Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single‐Cell Resolution by Taifeng Zhou, Yu Chen, Zhiheng Liao, Long Zhang, Deying Su, Zhuling Li, Xiaoming Yang, Xiaona Ke, Hengyu Liu, Yuyu Chen, Ricong Weng, Huimin Shen, Caixia Xu, Yong Wan, Ren Xu, Peiqiang Su

“…Although several studies have demonstrated the cellular heterogeneity of adult mature IVDs, a single‐cell transcriptomic atlas mapping early IVD formation is still lacking. …”
Get full text

Delayed Starch Degradation Triggers Chromoplast Structural Aberration to Inhibit Carotenoid Cleavage: A Novel Mechanism for Flower Color Deepening in <i>Osmanthus fragrans</i> by Xiangling Zeng, Yunfei Tan, Xin Wen, Qiang He, Hui Wu, Jingjing Zou, Jie Yang, Xuan Cai, Hongguo Chen

“…Transcriptomic analysis indicates that carotenoid metabolic pathway genes do not correlate directly with the phenotype; however, 49 candidate genes significantly associated with pigment accumulation were identified. …”
Get full text

Epigenetic determinants of an immune-evasive phenotype in HER2-low triple-negative breast cancer by Andrés F. Bedoya-López, Sookyung Ahn, Miquel Ensenyat-Mendez, Javier I. J. Orozco, Sandra Iñiguez-Muñoz, Pere Llinàs-Arias, Samantha M. Thomas, Jennifer L. Baker, Peggy S. Sullivan, Jitin Makker, Julie B. Steele, Sunil M. Kurian, Diego M. Marzese, Maggie L. DiNome

“…Abstract Identifying molecular drivers in triple-negative breast cancer (TNBC) is crucial. While HER2-low expression predicts response to novel antibody-drug conjugates, its biological influence on TNBC biology is unknown. …”
Get full text

Physiological and molecular responses of tomato and citrus to chromium (III) stress at early growth stage by Min Wang, Hangfei Li, Kai Xu, Jiaying Fang, Chao Yu, Weiwei Zheng, Haijie Ma

“…Citrus seed germination declines with increasing Cr(III) concentrations, while low Cr(III) levels promote tomato germination, with inhibition occurring above 1 g/L. …”
Get full text

Molecular characterisation of influenza B virus from the 2017/18 season in primary models of the human lung reveals improved adaptation to the lower respiratory tract by Duygu Merve Çalışkan, Sriram Kumar, Saskia Hinse, Klaus Schughart, Rainer Wiewrodt, Stefan Fischer, Vera Krueger, Karsten Wiebe, Peter Barth, Alexander Mellmann, Stephan Ludwig, Linda Brunotte

“…Instead of influenza A viruses, this season was dominated by infections with influenza B viruses of the Yamagata lineage. While this IBV/Yam dominance was associated with a vaccine mismatch, a contribution of virus intrinsic features to the clinical severity of the infections was speculated. …”
Get full text

Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration by Zhangrong Cheng, Haiyang Gao, Pengzhi Shi, Anran Zhang, Xianglong Chen, Yuhang Chen, Weikang Gan, Kangcheng Zhao, Shuai Li, Cao Yang, Yukun Zhang

“…Abstract Intervertebral disc degeneration (IDD) is a progressive and dynamic process in which the senescence-associated secretory phenotype (SASP) of nucleus pulposus cells (NPC) plays a significant role. While impaired chaperone-mediated autophagy (CMA) has been associated with inflammation and cellular senescence, its specific involvement in the self-perpetuating feedback loop of NPC senescence remains poorly understood. …”
Get full text

FGFR4 p.Gly388Arg polymorphism in PBMCs of LAM patients: findings of a pilot study by Sinem Koc-Gunel, Sinem Koc-Gunel, Amy L. Ryan, Melanie Winter, Thomas O. F. Wagner, Thomas O. F. Wagner

“…Patients with the variant exhibited significantly faster rates of lung function decline, as measured by FEV₁%, compared to those without the variant. Spatial transcriptomic analysis of LAM lung tissue revealed FGFR4 expression predominantly in alveolar fibroblasts and AT2 epithelial cells, key compartments in lung remodeling, while detection in PBMCs supports a potential systemic role. …”
Get full text

The coordinated regulatory impact of AcsS and TpdA on biofilm formation in Vibrio parahaemolyticus by Bin Ni, Jingyang Chang, Yining Zhou, Yining Zhou, Wanpeng Li, Wanpeng Li, Zhukang Tian, Zhukang Tian, Renfei Lu, Yiquan Zhang

“…Intracellular c-di-GMP quantification showed that AcsS elevates c-di-GMP levels, while TpdA reduces them, establishing a feedback loop. …”
Get full text

The unfolded protein response influences therapy outcome and disease progression in chronic lymphocytic leukaemia by Umair Tahir Khan, Kim Clarke, Gina Eagle, Melanie Oates, Peter Hillmen, Sandrine Jayne, Martin J. S. Dyer, Alex Phipps, Francesco Falciani, Rosalind E. Jenkins, Andrew R Pettitt

“…Abstract Since genomics, epigenomics and transcriptomics have provided only a partial explanation of chronic lymphocytic leukaemia (CLL) heterogeneity, and since concordance between mRNA and protein expression is incomplete, we related the CLL proteome to clinical outcome. …”
Get full text

BTLA agonist attenuates Th17-driven inflammation in a mouse model of steroid-resistant asthma by Christine Quach, Xin Li, Pedram Shafiei-Jahani, Meng Li, Stephen Shen, Doumet Georges Helou, Benjamin P. Hurrell, Pejman Soroosh, Omid Akbari

“…The effects of BTLA agonist treatment were assessed on airway hyperreactivity and lung inflammation.ResultsTranscriptomic analysis revealed increased HVEM expression and decreased BTLA expression, both critical immune regulators associated with stimulatory and inhibitory signaling, respectively. …”
Get full text