Mature tertiary lymphoid structures linked to HPV status and anti-PD-1 based chemoimmunotherapy response in head and neck squamous cell carcinoma by Lizao Zhang, Siqi Ren, Tianjun Lan, Ventin Marco, Niu Liu, Bin Wei, Yunsheng Chen, Jiaying Wu, Qunxing Li, Fan Wu, Peichia Lu, Jiahao Miao, Hsinyu Lin, Xinhui Wang, Jianglong Zhong, Jinsong Li, Song Fan

“…Single-cell and spatial transcriptomics further revealed distinct TLS compositions, with mature TLSs enriched in germinal center B cells, follicular helper T cells, and resident memory CD8 T cells, while immature TLSs contained FCRL4+ B cells and peripheral helper T cells. …”
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WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signaling by Jan Hendrik Voss, Zsombor Koszegi, Yining Yan, Emily Shorter, Lukas Grätz, Johanna T. Lanner, Davide Calebiro, Gunnar Schulte

“…Here, we use bioluminescence resonance energy transfer (BRET) to show that different WNT paralogs dynamically trigger FZD-LRP6 association. While WNT-induced receptor interaction was independent of C-terminal LRP6 phosphorylation, it was allosterically modulated by binding of the phosphoprotein Dishevelled (DVL) to FZD. …”
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Insights into KMT2A rearrangements in acute myeloid leukemia: from molecular characteristics to targeted therapies by Sara Zehtabcheh, Hamed Soleimani Samarkhazan, Marjan Asadi, Mitra Zabihi, Sahar Parkhideh, Mohammad Hossein Mohammadi

“…ML models, leveraging transcriptomic data, can predict KMT2A-r and identify biomarkers like LAMP5 and SKIDA1, improving risk stratification. …”
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Mapping the complexity and diversity of tertiary lymphoid structures in primary and peritoneal metastatic gastric cancer by Carolina Martinez-Ciarpaglini, Tanja D de Gruijl, Daniela S Thommen, Nicole C T van Grieken, Joep Grootjans, Sarah Derks, Tessa S Groen-van Schooten, Rosalia Franco Fernandez, Emma N Bos, Jens Seidel, Job Saris, Tania C Fleitas

“…We, therefore, hypothesize that immune cell composition and function of TLS depends on tumor location and the tumor immune environment.Methods Spatial transcriptomics and immunohistochemistry were used to characterize the phenotype of CD45+ immune cells inside and outside of TLS using archival resection specimens from GC primary tumors and peritoneal metastases.Results We identified significant intrapatient and interpatient diversity of the cellular composition and maturation status of TLS in GC. …”
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Avelumab induces greater Fc-Fc receptor-dependent natural killer cell activation and dendritic cell crosstalk compared to durvalumab by Nicole Osborne, Amit Rupani, Vladimir Makarov, Timothy A. Chan, Raghvendra M. Srivastava

“…Several FDA-approved anti-PD-L1 (programmed cell death ligand-1) monoclonal antibodies (mAbs) are used to treat cancer. While these mAbs primarily target and intercept PD-L1:PD-1 inhibitory signaling in T-cells, the Fc-domains of these mAbs are distinct, and the unique cellular cascades triggered by differing Fc-domains of PD-L1 mAbs have not been directly investigated. …”
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Single-nucleus RNA sequencing reveals distinct pathophysiological trophoblast signatures in spontaneous preterm birth subtypes by Cherilyn Uhm, Jianlei Gu, Weina Ju, Stephanie Pizzella, Hande Oktay, Joyce Yao-Chun Peng, Sararose Guariglia, Yong Liu, Hongyu Zhao, Yong Wang, Ramkumar Menon, Nanbert Zhong

“…Our findings revealed distinct trophoblast compositions with pPROM characterized predominantly by extravillous trophoblasts (EVTs), while sPTL showed an abundance of syncytiotrophoblasts (STBs). …”
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Annotation-free deep learning algorithm trained on hematoxylin & eosin images predicts epithelial-to-mesenchymal transition phenotype and endocrine response in estrogen receptor-po... by Kaimin Hu, Yinan Wu, Yajing Huang, Meiqi Zhou, Yanyan Wang, Xingru Huang

“…Our classifier achieved a predicting accuracy of 81.25%, and 88.7% slides labeled as endocrine resistant were predicted as the mesenchymal-phenotype, while 75.6% slides labeled as sensitive were predicted as the epithelial-phenotype. …”
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The dynamic genomes of Hydra and the anciently active repeat complement of animal chromosomes by Koto Kon-Nanjo, Tetsuo Kon, Tracy Chih-Ting Koubkova Yu, Diego Rodriguez-Terrones, Francisco Falcon, Daniel E. Martínez, Robert E. Steele, Elly Margaret Tanaka, Thomas W. Holstein, Oleg Simakov

“…This conservation has been tested by expansions of selfish DNA elements, in particular transposable elements (TEs). While comparative genomics studies have highlighted their diversity across animal genomes, common principles underlying their evolution along deeply conserved chromosomes have been elusive. …”
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A microbiota-derived metabolite, 3-phenyllactic acid, prolongs healthspan by enhancing mitochondrial function and stress resilience via SKN-1/ATFS-1 in C. elegans by Juewon Kim, Yunju Jo, Gyumin Lim, Yosep Ji, Jong-Hwa Roh, Wan-Gi Kim, Hyon-Seung Yi, Dong Wook Choi, Donghyun Cho, Dongryeol Ryu

“…Abstract The mechanisms underlying the impact of probiotic supplementation on health remain largely elusive. While previous studies primarily focus on the discovery of novel bioactive bacteria and alterations in the microbiome environment to explain potential probiotic effects, our research delves into the role of living Lactiplantibacillus (formerly known as Lactobacillus) and their conditioned media, highlighting that only the former, not dead bacteria, enhance the healthspan of Caenorhabditis elegans (C. elegans). …”
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Ferric Uptake Regulator Contributes to <i>Pseudomonas donghuensis</i> HYS-Induced Iron Metabolic Disruption in <i>Caenorhabditis elegans</i> by Donghao Gao, Liwen Shen, Yelong Lin, Shuo Huang, Zhixiong Xie

“…Specifically, Fur was critical for disrupting nematode iron metabolism, as <i>fur</i> deletion eliminated this effect. While Fur regulated two HYS siderophores, neither of them mediated in the iron metabolism disruption of <i>C. elegans</i>. …”
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Mitochondrial respiration atlas reveals differential changes in mitochondrial function across sex and age by Dylan C Sarver, Muzna Saqib, Fangluo Chen, G William Wong

“…Organ function declines with age, and large-scale transcriptomic analyses have highlighted differential aging trajectories across tissues. …”
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Longitudinal analysis of genetic and epigenetic changes in human pluripotent stem cells in the landscape of culture-induced abnormality by Yun-Jeong Kim, Byunghee Kang, Solbi Kweon, Sejin Oh, Dayeon Kim, Dayeon Gil, Hyeonji Lee, Jung-Hyun Kim, Ji Hyeon Ju, Tae-Young Roh, Chang Pyo Hong, Hyuk-Jin Cha

“…Therefore, we conducted comprehensive genomic, single-cell transcriptomic, and single-cell ATAC-seq analyses of an isogenic hESC model displaying definitive ‘culture-adapted phenotypes’. …”
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<i>Colletotrichum gloeosporioides</i> Swiftly Manipulates the Transcriptional Regulation in <i>Citrus sinensis</i> During the Early Infection Stage by Siyu Zhang, Xinyou Wang, Wei Zeng, Leijian Zhong, Xiaoyong Yuan, Zhigang Ouyang, Ruimin Li

“…In this study, we analyzed the physiological and transcriptomic changes in <i>Citrus sinensis</i> at different stages of incubation with <i>C. gloeosporioides</i>. …”
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Choroid plexus organoids reveal mechanisms of Streptococcus suis translocation at the blood-cerebrospinal fluid barrier by Tiantong Zhao, Laura Pellegrini, Bart van der Hee, Jos Boekhorst, Aline Fernandes, Sylvia Brugman, Peter van Baarlen, Jerry M. Wells

“…S. suis was able to replicate at the same rate in CSF and laboratory S. suis culture medium but reached a lower final density. The analysis of transcriptomes in ChP organoids after S. suis infection indicated inflammatory responses, while the addition of Plg further suggested extracellular matrix (ECM) remodeling. …”
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Cannabidiol potentiates p53-driven autophagic cell death in non-small cell lung cancer following DNA damage: a novel synergistic approach beyond canonical pathways by Youngsic Jeon, Taejung Kim, Hyukjoon Kwon, Young Nyun Park, Tae-Hyung Kwon, Min Hong, Kyung-Chul Choi, Jungyeob Ham, Young-Joo Kim

“…To understand this synergistic effect, we conducted extensive transcriptomic and proteomic profiling, which showed that the combination of CBD and etoposide upregulated genes associated with autophagic cell death while downregulating key oncogenes known to drive tumor progression. …”
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Genome-wide identification of the UGT genes family in Acer rubrum and role of ArUGT52 in anthocyanin biosynthesis under cold stress by Khan Arif Kamal, Faheem Afzal Shah, Yue Zhao, Zhu Chen, Songling Fu, Zhiyong Zhu, Jie Ren, Hua Liu

“…Compared to controls, 44 UGTs were upregulated and 99 downregulated in yellow leaves, while 59 were upregulated and 84 downregulated in red leaves. …”
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TGFβ signaling sensitizes MEKi-resistant human melanoma to targeted therapy-induced apoptosis by Benjamin Loos, Adrian Salas-Bastos, Anna Nordin, Julien Debbache, Salome Stierli, Phil F. Cheng, Stefanie Rufli, Conrad Wyss, Mitchell P. Levesque, Reinhard Dummer, Wendy Wei-Lynn Wong, Steve Pascolo, Claudio Cantù, Lukas Sommer

“…However, its involvement in the response to therapy is controversial. While several studies have linked TGFβ signaling to elevated resistance to targeted therapy in melanoma, separate findings have indicated a favorable treatment response through TGFβ-mediated increase of cell death. …”
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Melanin deposition and key molecular features in Xenopus tropicalis oocytes by Hongyang Yi, Weizheng Liang, Sumei Yang, Han Liu, Jiayu Deng, Shuhong Han, Xiaohui Feng, Wenjie Cheng, Yonglong Chen, Jing Hang, Hongzhou Lu, Rensen Ran

“…Additionally, in Xenopus tropicalis oocytes, the expression of the MiT subfamily factor tfe3 is relatively high, while tfeb, mitf, and tfec levels are extremely low. …”
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Deciphering the Complexity of the Immune Cell Landscape in Kidney Allograft Rejection by George Terinte-Balcan, George Terinte-Balcan, George Terinte-Balcan, Emilie Lebraud, Julien Zuber, Dany Anglicheau, Dany Anglicheau, Gener Ismail, Gener Ismail, Marion Rabant, Marion Rabant

“…In the last few years, multiplex IF techniques and automatic counting systems as well as transcriptomics studies (bulk, single-cell and spatial techniques) have provided invaluable clues to further decipher the complex puzzle of rejection. …”
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Root-to-shoot mobile mRNA CmoKARI1 promotes JA-Ile biosynthesis to confer chilling tolerance in grafted cucumbers by Miao Zhang, Wenqian Liu, Cuicui Wang, Shujin Lin, Ying Chen, Heran Cui, Chenggang Xiang, Yujia Ma, Xiaojun Li, Yongzhen Lu, Xiao Han, Yihan Dong, Lihong Gao, Wenna Zhang

“…Abstract Pumpkin rootstock is commonly used to graft cucurbit crops, improving their ability to withstand stress. While the significance of systemic signals from rootstocks to scions is recognized, the role of root-to-shoot transported mRNAs remains understudied. …”
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