Curative timed NK cell-based immunochemotherapy aborts brain tumour recurrence driven by mesenchymal glioma stem cells by Brian Meehan, Lata Adnani, Xianbing Zhu, Nadim Tawil, Delphine Garnier, Ichiro Nakano, Sidong Huang, Janusz Rak

“…Strikingly, molecular subtypes of HGG impacted the repertoire of NK cell sensitivity markers across human HGG transcriptomes, and in a panel of GSCs with either proneural (PN-GSC) or mesenchymal (MES-GSC) phenotypes. …”
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Enhanced Stress Tolerance in Rice Through Overexpression of a Chimeric Glycerol-3-Phosphate Dehydrogenase (OEGD) by Jinhong Wu, Meiyao Chen, Fangwen Yang, Jing Han, Xiaosong Ma, Tianfei Li, Hongyan Liu, Bin Liang, Shunwu Yu

“…Overexpression of <i>OEGD</i> in rice enhanced tolerance to drought, phosphorus deficiency, high temperature, and cadmium (Cd²⁺) stresses, while also improving plant growth and yield under drought stress at the adult stage. …”
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BRD4 sustains p63 transcriptional program in keratinocytes by E. Foffi, A. Violante, R. Pecorari, A. M. Lena, F. Rugolo, G. Melino, E. Candi

“…Our protein–protein interaction assays demonstrated a strong and conserved physical interaction between BRD4 and the p53 family members—p63, p73, and p53—suggesting a shared binding region among these proteins. While the role of BRD4 in cancer development through its interaction with p53 has been explored, the effects of BRD4 and Bromodomain and Extra Terminal (BET) inhibitors in non-transformed cells, such as keratinocytes, remain largely unknown. …”
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Identification of macrophage-associated diagnostic biomarkers and molecular subtypes in gestational diabetes mellitus based on machine learning by Kai Wei, Liyun Yuan, Yongsheng Ge, Han Yu, Guoping Zhao, Guoqing Zhang, Guohua Liu

“…For scRNA-seq data, cell clusters were annotated using the SingleR package and validated with marker gene expression profiles, while hdWGCNA analysis identified three gene modules related to macrophages. …”
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Thyroid‐Targeted Nano‐Bombs Empower HIFU for Graves' Disease by Binhao Wang, Zhuobing Yin, Xiangyue You, Hanwei Peng, Ying Jiang

“…A polydopamine coating enhances biocompatibility, while iodine loading and thyroid‐stimulating hormone receptor (TSHR) antibodies grafting ensure targeted delivery to the thyroid. …”
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Characterization of the aroma and flavor profiles of guava fruit (Psidium guajava) during developing by HS-SPME-GC/MS and RNA sequencing by Jie Zhang, Yi Zhang, Shuaiyu Zou, Endian Yang, Ziyi Lei, Tingting Xu, Chen Feng

“…The early developmental stages are more critical in influencing the variation of VOCs in the flesh, while VOC changes in peel occur more progressively across the developmental stages. …”
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HOX gene expression in the developing human spine by John E. G. Lawrence, Kenny Roberts, Elizabeth Tuck, Tong Li, Lira Mamanova, Petra Balogh, Inga Usher, Alice Piapi, Pavel Mazin, Nathaniel D. Anderson, Liam Bolt, Laura Richardson, Elena Prigmore, Xiaoling He, Roger A. Barker, Adrienne Flanagan, Matthew D. Young, Sarah A. Teichmann, Omer Bayraktar, Sam Behjati

“…Here, we use single-cell and spatial-transcriptomics, along with in-situ sequencing, to create a developmental atlas of the human fetal spine. …”
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Transcriptional Profiling Underscores the Role of Preprocurement Allograft Metabolism and Innate Immune Status on Outcomes in Human Liver Transplantation by Joohyun Kim, MD, PhD, Michael T. Zimmermann, PhD, Angela J. Mathison, PhD, Gwen Lomberk, PhD, Raul Urrutia, MD, Johnny C. Hong, MD

“…Therefore, we sought to characterize transcriptomic profiles of IRI during multiple phases in human OLT. …”
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Artificial intelligence-assisted RNA-binding protein signature for prognostic stratification and therapeutic guidance in breast cancer by Yunxia Zhao, Li Li, Shuqi Yuan, Zixin Meng, Jiayi Xu, Zhaogen Cai, Yijing Zhang, Xiaonan Zhang, Tao Wang

“…Low AIRS scores predicted better responses to immune checkpoint inhibitors, while drug sensitivity analysis highlighted panobinostat and paclitaxel as potential therapies for high-risk patients.ConclusionsThe AIRS model offers a robust tool for BC prognosis and treatment stratification, integrating genomic, transcriptomic, and single-cell data. …”
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Differential ABA sensitivity of superior and inferior rice grains is linked to cell cycle entry into endoreduplication by Anil Kumar Nalini Chandran, Larissa Irvin, Balpreet K. Dhatt, Yuvraj Chopra, Steven McArtney, Marci A. Surpin, Sriram K. Reddy, Harkamal Walia

“…Our results indicate that ABA alone promotes photosynthate remobilization into both superior and inferior grains while ABA + BA regulates grain filling via cell cycle-related transcriptomic changes. …”
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Multi-Omics Profiling Reveals Glycerolipid Metabolism-Associated Molecular Subtypes and Identifies ALDH2 as a Prognostic Biomarker in Pancreatic Cancer by Jifeng Liu, Shurong Ma, Dawei Deng, Yao Yang, Junchen Li, Yunshu Zhang, Peiyuan Yin, Dong Shang

“…<b>Results</b>: Pan-cancer analysis identified molecular features of GMRGs in cancers, while scRNA-seq, spatial transcriptomics, and lipidomics highlighted GLM heterogeneity in PC. …”
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Carboxymethyl chitosan oligosaccharide enzymatic hydroxylates with reactive oxygen species scavenging and anti-inflammatory activity for topical treatment of skin photodamage by Ziming Zhu, Hui Li, Xiansen Lv, Yan Yang, Baoqin Han, Zhiwen Jiang

“…In photodamaged HaCaT cells, CM-COS maintained cell viability and cytoskeletal integrity while inhibiting reactive oxygen species accumulation, apoptosis, and cell cycle arrest. …”
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5-Oxoproline prevents doxorubicin-induced cardiotoxicity and tumor growth by Xinning Guo, Meng Jiang, Zhengyu Tao, Huijuan Dai, Chen Wu, Yinan Wang, Zi Wang, Xiaoning Wang, Zhixuan Zhang, Kun Qian, Shanshan Zeng, Yihua Bei, Jun Pu

“…Given that alterations in metabolic pathways have been observed in both cardiovascular diseases and cancer, targeting specific metabolic pathways may offer dual benefits by mitigating DOX-induced cardiotoxicity while simultaneously enhancing its antitumor efficacy. …”
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IAA-Mediated Haustorium Formation in <i>Phelipanche aegyptiaca</i>: Transcriptional Insights and Anti-Parasitic Strategies by Xin Hu, Xiaolei Cao, Qiuyue Zhao, Xiaojian Zeng, Yingming Wei, Zhaoqun Yao, Sifeng Zhao

“…Here, we employed transcriptomics to investigate the molecular events in <i>P. aegyptiaca</i> haustorium formation induced by indole-3-acetic acid. …”
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Sequential emergence and contraction of epithelial subtypes in the prenatal human choroid plexus revealed by a stem cell model by Haley Masters, Shuxiong Wang, Christina Tu, Quy Nguyen, Yutong Sha, Matthew K. Karikomi, Pamela Shi Ru Fung, Benjamin Tran, Cristina Martel, Nellie Kwang, Michael Neel, Olga G. Jaime, Victoria Espericueta, Brett A. Johnson, Kai Kessenbrock, Qing Nie, Edwin S. Monuki

“…In simplified differentiations from human pluripotent stem cells, derived CPECs (dCPECs) display canonical properties and dynamic motile multiciliated phenotypes that interact with Aβ uptake. Single dCPEC transcriptomes over time correlate well with human organoid and fetal CPECs, while pseudotemporal and cell cycle analyses highlight the direct CPEC origin from neuroepithelial cells. …”
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Longitudinal multi-omics in alpha-synuclein Drosophila model discriminates disease- from age-associated pathologies in Parkinson’s disease by Justin Moore, Timothy Wu, Justin Dhindsa, Omar El Fadel, Anh Le, Alma Perez, Bismark Amoh, Akash Tarkunde, Katy F. Zhu, Matthew Avalos, Eric B. Dammer, Duc M. Duong, Nicholas T. Seyfried, Joshua M. Shulman, Ismael Al-Ramahi, Juan Botas

“…Unexpectedly, single-cell analysis at a mid-disease stage revealed that neurons upregulate protein synthesis and nonsense-mediated decay, while glia drive their overall downregulation. Longitudinal multi-omics approaches in animal models can thus help elucidate the molecular cascades underlying neurodegeneration vs. aging and co-pathologies.…”
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Expression of a humanized viral 2A-mediated lux operon efficiently generates autonomous bioluminescence in human cells. by Tingting Xu, Steven Ripp, Gary S Sayler, Dan M Close

“…<h4>Background</h4>Expression of autonomous bioluminescence from human cells was previously reported to be impossible, suggesting that all bioluminescent-based mammalian reporter systems must therefore require application of a potentially influential chemical substrate. While this was disproven when the bacterial luciferase (lux) cassette was demonstrated to function in a human cell, its expression required multiple genetic constructs, was functional in only a single cell type, and generated a significantly reduced signal compared to substrate-requiring systems. …”
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Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma by Ece Cakiroglu, Sude Eris, Ozden Oz, Gökhan Karakülah, Serif Senturk

“…Transcriptomic profiling of BUB1-depleted cells discloses differential gene expression signatures congruent with cell fate phenotypes, including the reprogramming of mitotic network genes. …”
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Aspergillus fumigatus calcium-responsive transcription factors regulate cell wall architecture promoting stress tolerance, virulence and caspofungin resistance. by Patrícia Alves de Castro, Ana Cristina Colabardini, Adriana Oliveira Manfiolli, Jéssica Chiaratto, Lilian Pereira Silva, Eliciane Cevolani Mattos, Giuseppe Palmisano, Fausto Almeida, Gabriela Felix Persinoti, Laure Nicolas Annick Ries, Laura Mellado, Marina Campos Rocha, Michael Bromley, Roberto Nascimento Silva, Gabriel Scalini de Souza, Flávio Vieira Loures, Iran Malavazi, Neil Andrew Brown, Gustavo H Goldman

“…The ΔzipD mutant displayed altered cell wall organization and composition, while being more susceptible to macrophage killing and eliciting an increased pro-inflammatory cytokine response. …”
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A deep learning model to predict glioma recurrence using integrated genomic and clinical data by Jessica A. Patricoski-Chavez, Seema Nagpal, Ritambhara Singh, Jeremy L. Warner, Ece D. Gamsiz Uzun

“…The prognosis varies considerably; patients with low-grade gliomas (LGGs) have 5-year survival rates of up to 80%, while patients with higher-grade gliomas (HGGs) often experience rates below 5%. …”
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