Sudapyridine (WX-081) inhibits Mycobacterium tuberculosis by targeting ATP synthase and upregulating host innate immunity by Xinda Li, Xiaoyi Luo, Bin Wang, Lei Fu, Xi Chen, Yu Lu

“…This study reveals WX-081’s dual mechanisms: targeting atpE to disrupt ATP synthase and proton motive force via resistance screening, gene sequencing, and functional assays while enhancing host immunity through macrophage transcriptomics. …”
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Chromogranin A deficiency attenuates tauopathy by altering epinephrine–alpha-adrenergic receptor signaling in PS19 mice by Suborno Jati, Daniel Munoz-Mayorga, Shandy Shahabi, Kechun Tang, Yuren Tao, Dennis W. Dickson, Irene Litvan, Gourisankar Ghosh, Sushil K. Mahata, Xu Chen

“…Treatment of WT hippocampal organotypic slice cultures with EPI or Adra1 agonist promoted, while an Adra1 antagonist inhibited Tau hyperphosphorylation and formation of neurofibrillary tangles, which is unaltered upon CgA depletion. …”
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Stochastic variation in the FOXM1 transcription program mediates replication stress tolerance by Hendrika A. Segeren, Kathryn A. Wierenga, Frank M. Riemers, Elsbeth A. vanLiere, Bart Westendorp

“…We identified 37 genes differentially expressed between tolerant and sensitive cells, including several FOXM1 targets. While complete knockdown of FOXM1 impeded cell proliferation, partial knockdown protected cells against DNA damage, and improved recovery from drug‐induced RS. …”
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Functional Characterization of <i>TNFα</i> in the Starry Flounder (<i>Platichthys stellatus</i>) and Its Potential as an Immunostimulant by Min-Young Sohn, Gyoungsik Kang, Kyung-Ho Kim, Ha-Jeong Son, Chan-Il Park

“…Peak activity was observed at 150–200 μg/mL, while a decline at higher concentrations suggested a threshold for immune stimulation. …”
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Intercropping Forage Mulberry Benefits Nodulation and Growth of Soybeans by Xinjie Feng, Minghui Zhong, Xuexian Zhao, Xiuli Zhang, Yanbo Hu, Huihui Zhang

“…It also increased soil available phosphorus, soil available potassium and soil water content, while reducing soil available nitrogen and the pH value. …”
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Vitamin K2 inhibits PGE2-mediated osteoblast ferroptosis by upregulation of CBR1 via the Nrf2/Keap1 pathway by Lin Tao, Hongyang Li, Jinpeng Wang, Qichang Liu, Wenhai Cao, Yue Zhu

“…The mechanism and target of vitamin K2 therapy for osteoporosis was investigated through metabolomics and transcriptomics sequencing. Molecular docking was employed to identify downstream target proteins of vitamin K2, while EMSA and ChIP-qPCR were used to explore the specific molecular mechanisms. …”
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An Intelligent and Conductive Hydrogel with Multiresponsive and ROS Scavenging Properties for Infection Prevention and Anti‐Inflammatory Treatment Assisted by Electrical Stimulatio... by Tao Zhang, Zongwu Meng, Haoyu Yu, Ping Ding, Tianhan Kai

“…Furthermore, the HEPP integrates photodynamic therapy (PDT), photothermal therapy (PTT), and self‐cascading reactive oxygen species (ROS) to prevent bacterial infections while ensuring a continuous supply of oxygen (O2) to the wound. …”
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Human iPSC-derived spinal neural progenitors enhance sensorimotor recovery in spinal cord-injured NOD-SCID mice via differentiation and microenvironment regulation by Xuanbao Yao, Kehua Zhang, Tao Na, Yuchun Wang, Yuhan Guo, Jiajie Xi, Xiang Li, Shufang Meng, Miao Xu

“…Additionally, spNPGs integrated into host neural circuits, enhancing synaptic connectivity, while simultaneously modulating the injury microenvironment by shifting microglia and astrocyte polarization toward anti-inflammatory and neuroprotective phenotypes. …”
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Root growth and branching are enabled by brassinosteroid-regulated growth anisotropy and carbon allocation by Hitaishi Khandal, Guy Horev, Bas van den Herik, Yoram Soroka, Tamar Lahav, Tamar Avin-Wittenberg, Kirsten ten Tusscher, Sigal Savaldi-Goldstein

“…Here, we directly tackle this question using micro-grafts between wild-type and BR-null mutants in both Arabidopsis and tomato, assisted by phenotyping, transcriptomics, metabolic profiling, transmission electron microscopy, and modeling approaches. …”
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Machine learning based anoikis signature predicts personalized treatment strategy of breast cancer by Xiao Guo, Jiaying Xing, Yuyan Cao, Wenchuang Yang, Xinlin Shi, Runhong Mu, Tao Wang

“…Patients with low AIDAS levels were found to be more responsive to immunotherapies, including PD-1/PD-L1 inhibitors, while high-AIDAS patients demonstrated greater susceptibility to specific chemotherapeutic agents, such as methotrexate.ConclusionsThese findings highlight the critical role of anoikis in breast cancer prognosis and underscore AIDAS’s potential to guide individualized treatment strategies. …”
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Targeting alveolar epithelial cell metabolism in pulmonary fibrosis: Pioneering an emerging therapeutic strategy by Tengkun Dai, Tengkun Dai, Yidan Liang, Yidan Liang, Xin Li, Xin Li, Jiamin Zhao, Jiamin Zhao, Guangqin Li, Qihong Li, Lin Xu, Lin Xu, Juanjuan Zhao, Juanjuan Zhao

“…Metabolic reprogramming of AECs affects cellular senescence, endoplasmic reticulum stress, and oxidative stress in AECs, while also promoting fibrotic progression through various signaling pathways. …”
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70% ethanol preserves mycobacterial RNA from cultures more efficiently than GTC-TCEP by Lena Krausser, Magalie Van Dyck-Lippens, Ramata Balde, Rabab Reenaers, Reem Al Mubarak, Leen Rigouts, Nicholas D. Walter, Martin I. Voskuil, Annelies Van Rie, Bouke C. de Jong, Sofie M. Braet

“…Both buffers are reportedly mycobactericidal and, in this study, prevented heat stress-induced transcriptomic changes, thereby conserving a transcriptomic snapshot. …”
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An in vitro neurobacterial interface reveals direct modulation of neuronal function by gut bacteria by Juan Lombardo-Hernandez, Jesús Mansilla-Guardiola, Riccardo Aucello, Cristian Botta, Maria Teresa García-Esteban, Antonio Murciano-Cespedosa, David Muñoz-Rodríguez, Elisa Quarta, Álvaro Mateos González, Carmen Juan-Llamas, Kalliopi Rantsiou, Stefano Geuna, Luca Cocolin, Celia Herrera-Rincon

“…Abstract Interactions between bacteria and somatic cells are increasingly important for understanding cellular communication mechanisms. While the gut microbiome’s influence on the gut–brain axis is established, direct interactions between bacteria and neurons are poorly explored, especially regarding bidirectional information exchange. …”
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Genomic identification of recipient-derived hepatocytes in liver transplantation: a hypothesis linking graft repopulation to immune tolerance by Seoung Hoon Kim

“…BackgroundOperational tolerance, defined as stable liver graft function without immunosuppression, has been observed in select transplant recipients. While immune regulatory mechanisms have been implicated, the biological processes underlying tolerance remain incompletely understood. …”
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Biology and Application of <i>Chaetomium globosum</i> as a Biocontrol Agent: Current Status and Future Prospects by Shailja Sharma, Saurabh Pandey, Sourabh Kulshreshtha, Mukesh Dubey

“…In recent years, <i>C. globosum</i> has attracted considerable scientific interest due to its potential as a biocontrol agent [BCA] against a wide array of diseases in numerous plant species. While the precise mechanisms of <i>C. globosum</i> as a BCA remain poorly understood, interference competition through antibiosis is one of the key mechanisms. …”
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Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva by Léa Torcq, Anne Schmidt

“…Here, we provide experimental details of a protocol we have recently optimized to identify, based on mRNA in situ hybridization, precursors of hematopoietic stem cells while still embedded in the aortic wall (at the embryonic stage) as well as when they reside in specific niches a few days after emergence (at the early larval stage). …”
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Fungal bioremediation in environmental pollution and recent strategies by Linthoingambi Ningombam, Techi Mana, Srimay Pradhan, Gemin Apum, Yengkhom Disco Singh

“…Importantly, this review outlines the current challenges in scalability, substrate specificity, and bioreactor design, while suggesting future research directions, including genetic modification and optimization of consortia compositions. …”
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Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo Pig by Zunqiang Yan, Qiaoli Yang, Pengfei Wang, Shuangbao Gun

“…In this research, we performed 10× Genomics scRNA-seq to conduct an unbiased single-cell transcriptomic analysis in Hezuo pig (HZP) testis at one month of age during prepuberty. …”
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Identification of Anticancer Target Combinations to Treat Pancreatic Cancer and Its Associated Cachexia Using Constraint-Based Modeling by Feng-Sheng Wang, Ching-Kai Wu, Kuang-Tse Huang

“…A fuzzy multi-objective optimization framework was employed within the anticancer target discovery platform to evaluate cell viability and metabolic deviation as dual criteria for assessing therapeutic efficacy and potential side effects. While single-enzyme targets were found to be context-specific and medium-dependent, eight combinatorial targets demonstrated robust, medium-independent effects in both PDAC and PDAC-CX cells. …”
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Investigation of the Roles of Phosphatidylinositol 4-Phosphate 5-Kinases 7,9 and Wall-Associated Kinases 1–3 in Responses to Indole-3-Carbinol and Biotic Stress in Arabidopsis Thal... by Hala Khamesa-Israelov, Alin Finkelstein, Eilon Shani, Daniel A. Chamovitz

“…The first line, <i>ICT2</i>, targets the phosphatidylinositol 4-phosphate 5-kinase family (PIP5K), exhibiting tolerance to I3C, while the second line, <i>ICS1</i>, targets the Wall-Associated Kinases (WAK1–3) family, showing susceptibility to I3C. …”
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