Improved radicchio seedling growth under CsPbI3 perovskite rooftop in a laboratory-scale greenhouse for Agrivoltaics application by Carlo Spampinato, Salvatore Valastro, Gaetano Calogero, Emanuele Smecca, Giovanni Mannino, Valentina Arena, Raffaella Balestrini, Fabiano Sillo, Lucio Ciná, Antonino La Magna, Alessandra Alberti

“…Eu-enriched CsPbI3 layers are chosen to provide semi-transparency and phase stability while radicchio has limited size and grows in pots. …”
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Ocular immune privilege in action: The living eye imposes unique regulatory and anergic gene signatures on uveitogenic T cells by Zixuan Peng, Vijayaraj Nagarajan, Reiko Horai, Yingyos Jittayasothorn, Mary J. Mattapallil, Rachel R. Caspi

“…Using an in vivo immune privilege model, we previously reported that all naive retina-specific T cells entering the eye become primed in situ; about 30% become Foxp3+ regulatory T cells (Tregs), while the rest fail to induce pathology. Here, single-cell transcriptomics and functional validation revealed distinct phenotypes in both populations: ocular Tregs were highly suppressive, whereas non-Tregs expressed suppression- and anergy-associated genes and lacked regulatory function. …”
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Loss of UBE3A impacts both neuronal and non-neuronal cells in human cerebral organoids by R. Chris Estridge, Z. Begum Yagci, Dilara Sen, Tyler J. Johnson, Gautami R. Kelkar, Travis S. Ptacek, Jeremy M. Simon, Albert J. Keung

“…In this study, scRNAseq on organoids reveals the effects of UBE3A disruption on cell type-specific compositions and transcriptomic alterations. In the absence of UBE3A, progenitor proliferation and structures are disrupted while organoid composition shifts away from proliferative cell types. …”
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Chromatin landscape at cis-regulatory elements orchestrates cell fate decisions in early embryogenesis by Francesco Cardamone, Annamaria Piva, Eva Löser, Bastian Eichenberger, Mari Carmen Romero-Mulero, Fides Zenk, Emily J. Shields, Nina Cabezas-Wallscheid, Roberto Bonasio, Guido Tiana, Yinxiu Zhan, Nicola Iovino

“…Using 10× Multiome, we simultaneously analyzed the in vivo epigenomic and transcriptomic states of wild-type, E(z)-, and CBP-depleted embryos during zygotic genome activation at single-cell resolution. …”
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Single cell analysis identifies distinct CD4 + T cells associated with the pathobiology of pediatric obesity related asthma by David A. Thompson, Yvonne B. Wabara, Sarai Duran, Anna Reichenbach, Laura Chen, Kayla Collado, Changsuek Yon, John M. Greally DMed, Deepa Rastogi

“…NR3C1, coding for the glucocorticoid receptor, was downregulated, while genes coding for NLRP3 inflammasome were upregulated, in clusters with CDC42 upregulation and Th1 responses. …”
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Single-cell RNA sequencing using split-pool barcoding reveals transcriptional heterogeneity in Porphyromonas gingivalis with implications for periodontal pathogenesis by Eun-Young Jang, Seok Bin Yang, Jeewan Chun, Kyu Hwan Kwack, Sang-Wook Kang, Jae-Hyung Lee, Ji-Hoi Moon

“…Background Porphyromonas gingivalis is a keystone pathogen in periodontitis, associated with dysbiosis and chronic inflammation. While its virulence mechanisms are well characterized, its transcriptional heterogeneity at the single-cell level remains unexplored.Materials and Methods We applied split-pool barcoding-based single-cell RNA sequencing to profile gene expression in 1,942 individual P. gingivalis W83 cells cultured under anaerobic conditions. …”
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Precise genetic control of ATOH1 enhances maturation of regenerated hair cells in the mature mouse utricle by Tian Wang, Tian Yang, Amanda Kedaigle, Gabriela Pregernig, Ryan McCarthy, Ben Holmes, Xudong Wu, Lars Becker, Ning Pan, Kathy So, Leon Chen, Jun He, Ahmad Mahmoudi, Soumya Negi, Monika Kowalczyk, Tyler Gibson, Noah Druckenbrod, Alan G. Cheng, Joseph Burns

“…In utricles damaged ex vivo, both CMV-ATOH1 and GFAP-ATOH1 increased regeneration more effectively than RLBP1-ATOH1, while GFAP-ATOH1 and RLBP1-ATOH1 induced hair cells with more mature transcriptomes. …”
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Epigenetic insights into physiological resilience: Multigenerational readouts of CO2-induced seawater acidification effects on fish embryos by Tzu-Yen Liu, Jia-Jiun Yan, Ying-Jey Guh, Oki Hayasaka, Li-Yih Lin, Pung-Pung Hwang, Guan-Chung Wu, Ming-Tsung Chung, Yung-Che Tseng

“…Metabolic responses were generation-specific: F0 embryos displayed decreased ammonium excretion, while F1 and F2 maintained stable profiles. Transcriptomic analysis revealed generational changes in neurotransmission, ion regulation, and epigenetic pathways. …”
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Unique immune and other responses of human nasal epithelial cells infected with H5N1 avian influenza virus compared to seasonal human influenza A and B viruses by Kai Sen Tan, Jing Liu, Anand Kumar Andiappan, Zhe Zhang Ryan Lew, Ting Ting He, Hsiao Hui Ong, Douglas Jie Wen Tay, Zhen Qin Aw, Bowen Yi, Arfah Mohd Fauzi, Thinesshwary Yogarajah, Lee Ching Pei Carmen, Justin Jang Hann Chu, Vincent T. Chow, Mookkan Prabakaran, De-Yun Wang

“…The infected cells were subjected to virologic, transcriptomic and secretory protein analyses. While less adapted to infecting the nasal epithelium, HPAI H5N1 elicited unique host responses unlike seasonal influenza. …”
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Exercise training remodels inter-organ endocrine networks by Cheehoon Ahn, Andrea L. Hevener, Laurie J. Goodyear, Sue C. Bodine, Karyn A. Esser, Marcus M. Seldin, Lauren M. Sparks

“…Subcutaneous white adipose tissue emerged as a key endocrine regulator impacted by training, while extracellular matrix-derived factors were identified as globally regulated secretory features in trained vs sedentary animals. …”
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Single-nucleus neuronal transcriptional profiling of male C. elegans uncovers regulators of sex-specific and sex-shared behaviors by Katherine S. Morillo, Jonathan St. Ange, Yifei Weng, Rachel Kaletsky, Coleen T. Murphy

“…Our data demonstrate sex-shared neurons exhibit substantial heterogeneity between the sexes, while sex-specific neurons repurpose conserved molecular pathways to regulate dimorphic behaviors. …”
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From Multi‐Omics to Visualization and Beyond: Bridging Micro and Macro Insights in CAR‐T Cell Therapy by Yuting Gong, Peng Fei, Yicheng Zhang, Yang Xu, Jia Wei

“…The integration of genomics, epigenomics, transcriptomics, proteomics, metabolomics, and microbiomics enables a comprehensive understanding of the intrinsic mechanisms underlying CAR‐T therapy, while single‐cell and spatial omics significantly improve data resolution and analytical depth. …”
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Expression and function of Connexin 43 and Connexin 37 in the murine zona glomerulosa by Gabriel Stölting, Nicole Hellmig, Hoang An Dinh, Frederike Butz, Ali Kerim Secener, Marina Volkert, Ute I. Scholl

“…Based on published transcriptomics data, we re‐examined the presence and functional impact of GJ in the ZG. …”
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Comparative Analysis of the Biochemical and Molecular Responses of <i>Nannochloropsis gaditana</i> to Nitrogen and Phosphorus Limitation: Phosphorus Limitation Enhances Carotenogen... by Sun Young Kim, Hanbi Moon, Yong Min Kwon, Kyung Woo Kim, Jaoon Young Hwan Kim

“…These results were generally consistent with the gene expression pattern observed in transcriptomic analysis. This integrated analysis shows that phosphorus limitation can be an economically competitive solution by enhancing valuable carotenoids while maintaining lipid and biomass production in <i>N. gaditana</i>.…”
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<i>CdGLK1</i> Transcription Factor Confers Low-Light Tolerance in Bermudagrass via Coordinated Upregulation of Photosynthetic Genes and Enhanced Antioxidant Enzyme Activity by Peng Han, Jun Liu, Jingjin Yu, Zhongpeng Liu, Fahui He, Zhimin Yang

“…As a widely cultivated warm-season turfgrass, bermudagrass (<i>Cynodon</i> spp.) faces significant challenges in shaded environments due to its inherent low-light sensitivity. While improving photosynthetic adaptation represents a promising strategy to address this limitation, the associated regulatory mechanisms remain insufficiently characterized. …”
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The CoREST complex is a therapeutic vulnerability in malignant peripheral nerve sheath tumors by Imad Soukar, Robert J. Fisher, Sanjana Bhagavatula, Marianne Collard, Philip A. Cole, Rhoda M. Alani

“…Abstract Malignant peripheral nerve sheath tumor (MPNST) is a highly aggressive sarcoma that may be seen in patients with neurofibromatosis type 1 (NF1) or occur sporadically. While surgery is the primary treatment for localized MPNST with a 61.9% overall survival rate, metastatic disease is often fatal due to resistance to systemic therapies which underscores the urgent need for effective treatments. …”
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Development and pan-cancer validation of an epigenetics-based random survival forest model for prognosis prediction and drug response in OS by Chaoyi Yin, Kede Chi, Zhiqing Chen, Shabin Zhuang, Yongsheng Ye, Binshan Zhang, Cailiang Cai

“…BackgroundOsteosarcoma (OS) exhibits significant epigenetic heterogeneity, yet its systematic characterization and clinical implications remain largely unexplored.MethodsWe analyzed single-cell transcriptomes of five primary OS samples, identifying cell type-specific epigenetic features and their evolutionary trajectories. …”
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Regulation of HSC development and function by Lin28b by Grant Cox, Michihiro Kobayashi, Brian D. Rudd, Momoko Yoshimoto

“…Hematopoietic stem cells (HSCs) provide all kinds of blood cells for life while maintaining self-renewal ability. During development, HSCs are first produced in the mouse embryo around embryonic day (E) 11. …”
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BaCoN (Balanced Correlation Network) improves prediction of gene buffering by Thomas Rohde, Talip Yasir Demirtas, Sebastian Süsser, Angela Helen Shaw, Manuel Kaulich, Maximilian Billmann

“…We quantified 147 million potential buffering relationships by associating CRISPR-Cas9-screening fitness effects with transcriptomic data across 1019 Cancer Dependency Map (DepMap) cell lines. …”
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Bioinformatic RNA-Seq Functional Profiling of the Tumor Suppressor Gene OPCML in Ovarian Cancers: The Multifunctional, Pleiotropic Impacts of Having Three Ig Domains by Adam G. Marsh, Franziska Görtler, Sassan Hafizi, Hani Gabra

“…These results emphasize the role of OPCML’s regulation of receptor tyrosine kinase (RTK) signaling pathways (PI3K/AKT and MEK/ERK) while identifying three new potential RTK transcriptomic linkages to KIT, TEK, and ROS1 in ovarian cancer. …”
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