Stepwise strategy for generating human trophoblast stem-like cells from embryonic stem cells reveals specific role of TFAP2C in acquiring self-renewability by Masatoshi Ohgushi, Kaori Honda, Rina Takagi, Mototsugu Eiraku

“…Notably, while GATA3+ cells lack self-renewal capacity, the TSC culture conditions facilitate their conversion into self-renewing TSLCs. …”
Get full text

Integration of single-cell sequencing and mendelian randomization reveals novel causal pathways between monocytes and hepatocellular carcinoma by Jiang Yu, Jing Yu, Zhou Kang, Yong Peng

“…Within the tumor microenvironment (TME), monocytes play a dual role: they participate in tumor cell recognition and elimination while regulating immune responses through cytokine secretion. …”
Get full text

Prioritizing perturbation-responsive gene patterns using interpretable deep learning by Yan Cui, Zhiyuan Yuan

“…Abstract Spatially resolved transcriptomics enables mapping of multiplexed gene expression within tissue contexts. …”
Get full text

The BeeBiome data portal provides easy access to bee microbiome information by Valentine Rech de Laval, Benjamin Dainat, Philippe Engel, Marc Robinson-Rechavi

“…While each of these datasets by itself has provided important insights, the integration of such datasets provides an unprecedented opportunity to obtain a global picture of the microbes associated with bees and their link to bee health. …”
Get full text

LIF, a mitogen for choroidal endothelial cells, protects the choriocapillaris: implications for prevention of geographic atrophy by Pin Li, Qin Li, Nilima Biswas, Hong Xin, Tanja Diemer, Lixian Liu, Lorena Perez Gutierrez, Giovanni Paternostro, Carlo Piermarocchi, Sergii Domanskyi, Ruikang K Wang, Napoleone Ferrara

“…LIF stimulated growth of BCE while it inhibited, as previously reported, bovine aortic EC (BAE) growth. …”
Get full text

Integrated multi-omics reveals flavonoid-dominated defense strategies in Coptis chinensis under Fusarium root rot infection by Kaidi Yu, Yuying Yang, Yuqing Duan, Jinwen You, Jie Guo

“…This study uncovers flavonoid biosynthesis as the primary defense strategy in C. chinensis during root rot progression, while SA signaling may not be the main defense mechanism. …”
Get full text

Single-Cell Atlas of Spleen Remodeling Reveals Macrophage Subset-Driven ASFV Pathogenesis by Liyuan Wang, Shouzhang Sun, Lei Liu, Yun Chen, Haixue Zheng, Zhonglin Tang

“…Viral gene expression analysis revealed E165R as a central hub in viral replication networks, while host transcriptomics uncovered disruption of Netrin signaling pathways that may facilitate immune evasion. …”
Get full text

Identification of ulcerative colitis diagnostic markers from differentially expressed genes shared with Hirschsprung disease by Wei Zuo, Zhengguang Wang

“…Pearson correlation analysis evaluated the relationship between immune cell infiltration and feature genes, while a Protein-Protein Interaction (PPI) network examined interactions with related proteins. …”
Get full text

Tick HRF-dependent ferroptosis pathway to promote tick acquisition of Babesia microti by Songqin Chen, Shanming Hu, Yongzhi Zhou, Jie Cao, Houshuang Zhang, Yanan Wang, Jinlin Zhou

“…In vivo studies demonstrated that the ferroptosis promoter Erastin increased B. microti load (p<0.05), while the inhibitor Ferrostatin-1 effectively decreased load (p<0.01). …”
Get full text

Phenotypic and transcriptional fidelity of patient-derived colon cancer xenografts in immune-deficient mice. by Jeffrey Chou, Matthew P Fitzgibbon, Christie-Lynn L Mortales, Andrea M H Towlerton, Melissa P Upton, Raymond S Yeung, Martin W McIntosh, Edus H Warren

“…In most but not all cases, the human stroma, vasculature, and hematopoietic elements were systematically replaced by murine analogues while the carcinoma component persisted. Once established as xenografts, human CRC cells that could be propagated by serial transplantation remained transcriptionally stable. …”
Get full text

Advances in tumor subclone formation and mechanisms of growth and invasion by Yuhong Zhang, Weidong Wang

“…Major methodologies for studying tumor subclones include single-cell sequencing, liquid biopsy, and spatial transcriptomics, which provide insights into clonal architecture and dynamic evolution. …”
Get full text

Microglial intervention in ischemic stroke: Roles and intervention strategies by Cuiling Ji, Lixinbei Sheng, Kaijun Han, Ping Yuan, Wei Li, Lu Chen, Yongyue Gao

“…Activated microglia contribute to neuroinflammation through cytokine release and disruption of the blood–brain barrier, while simultaneously promoting tissue repair through anti-inflammatory responses and regeneration. …”
Get full text

Cross-species transmission of an ancient endogenous retrovirus and convergent co-option of its envelope gene in two mammalian orders. by J'Zaria Simpson, Christine A Kozak, Guney Boso

“…ERVs thus represent molecular fossil records of ancient retroviruses and provide a unique opportunity to study viral-host interactions, including cross-species transmissions, in deep time. While most ERVs contain the mutated remains of the original retrovirus, on rare occasions evolutionary selection pressures lead to the co-option/exaptation of ERV genes for a host function. …”
Get full text

Lineage trajectories and fate determinants of postnatal neural stem cells and ependymal cells in the developing ventricular zone. by Jianqun Zheng, Yawen Chen, Yukun Hu, Yujian Zhu, Jie Lin, Manlin Xu, Yunlong Zhang, Weihong Song, Xi Chen

“…Previous lineage tracing studies showed that both NSCs and EPCs were derived from radial glial cells (RGCs). However, the transcriptomic dynamics and the molecular mechanisms guiding the cell fate commitment during the differentiation remain poorly understood. …”
Get full text

ScienceArt Commune 1: Whirling with Waters by Joseph Tin Yum Wong

“…Some cells are closer to the surface with higher irradiance and exposure, while others are screened by the bloom and face potential anaerobic conditions near the bottom. …”
Get full text

Id2 levels determine the development of effector vs. exhausted tissue-resident memory CD8+ T cells during CNS chronic infection by Aboubacar Sidiki K. Coulibaly, Lucie Nozeran, Céline Thomann, Marine Alis, Emilie Bassot, Ali Hassan, Rémi Porte, Marcy Belloy, Nicolas Blanchard, Frederick Masson

“…Although single-cell transcriptomics have revealed Trm heterogeneity in various diseases, the molecular mechanisms behind this diversity are unclear. …”
Get full text

Skeletal muscle lncRNA profile associated with fatty acids in Nellore beef cattle by Bruna Maria Salatta, Maria Malane Magalhães Muniz, Larissa Fernanda Simielli Fonseca, Lucio Flavio Macedo Mota, Caio de Souza Teixeira, Gabriela Bonfá Frezarim, Marta Serna-García, Danielly Beraldo dos Santos Silva, Angélica Simone Cravo Pereira, Fernando Baldi, Lucia Galvão de Albuquerque

“…The proportion of SFA, myristic, palmitic and stearic were significantly (p ≤ 0.05) higher in C3, while C2 presented an intermediate profile. DE analyses were performed on three different comparisons, C1 vs. …”
Get full text

Selective metabolic regulations by p53 mutant variants in pancreatic cancer by Sabrina Caporali, Alessio Butera, Alessia Ruzza, Carlotta Zampieri, Marina Bantula’, Sandra Scharsich, Anna-Katerina Ückert, Ivana Celardo, Ian U. Kouzel, Luigi Leanza, Andreas Gruber, Joan Montero, Angelo D’Alessandro, Thomas Brunner, Marcel Leist, Ivano Amelio

“…Conversely, p53R172H, while not affecting mitochondrial metabolism, attenuates the activation of pro-tumorigenic metabolic pathways such as the urea cycle. …”
Get full text

Oncolytic adenovirus decreases the proportion of TIM-3+ subset of tumor-infiltrating CD8+ T cells with correlation to improved survival in patients with cancer by Matti Kankainen, Timo Joensuu, Akseli Hemminki, Juuso Juhila, Siri Tähtinen, Otto Hemminki, Anna Kanerva, Kristian Taipale, Ilkka Liikanen, Minna Oksanen, Dafne C A Quixabeira, Saru Basnet

“…Following patient stratification with regard to TIM-3 (increase vs decrease in tumors), overall survival and imaging/marker responses were evaluated by log-rank and Fisher’s test, while coinhibitory receptors/ligands, transcriptomic changes and tumor-reactive and tumor-infltrating immune cells in biopsies and blood samples were studied by microarray rank-based statistics and immunoassays.Results Preclinically, TIM-3+ tumor-infiltrating lymphocytes (TILs) in B16 melanoma showed an exhausted phenotype, whereas oncolytic adenovirus treatment significantly reduced the proportion of TIM-3+ TIL subset through recruitment of less-exhausted CD8+ TIL. …”
Get full text

scRL: Utilizing Reinforcement Learning to Evaluate Fate Decisions in Single-Cell Data by Zeyu Fu, Chunlin Chen, Song Wang, Junping Wang, Shilei Chen

“…Single-cell RNA sequencing now profiles whole transcriptomes for hundreds of thousands of cells, yet existing trajectory-inference tools rarely pinpoint where and when fate decisions are made. …”
Get full text