Determining signalling nodes for apoptosis by a genetic high-throughput screen. by Bevan Lin, Derek Huntley, Ghada Abuali, Sarah R Langley, George Sindelar, Enrico Petretto, Sarah Butcher, Stefan Grimm

“…<h4>Methodology/principal findings</h4>We have set up a high-throughput screen in 96-well microtiter plates for genes that induce apoptosis upon their individual transfection into human cells. …”
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Transgelin-2 is upregulated on activated B-cells and expressed in hyperplastic follicles in lupus erythematosus patients. by Kaori Kiso, Hajime Yoshifuji, Takuma Oku, Masaki Hikida, Koji Kitagori, Yoshitaka Hirayama, Toshiki Nakajima, Hironori Haga, Tatsuaki Tsuruyama, Aya Miyagawa-Hayashino

“…TAGLN2-knockdown in Raji cells resulted in impaired phosphorylation of PLCγ2 leading to inhibition of cell migration. Microarray analysis of TAGLN2-knockdown Raji cells showed decreased expression of the genes associated with immune function including CCR6 and as well as of those associated with regulation of the actin cytoskeleton including ABI2, compared to controls. …”
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Extracellular vesicle-packaged miR-4253 secreted by cancer-associated fibroblasts facilitates cell proliferation in gastric cancer by inducing macrophage M2 polarization by Xinxing Duan, Xiong Yu, Jin Gan

“…The molecular mechanism was explored using microarray analysis, dual-luciferase reporter assay, and RNA pull-down analysis. …”
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Advanced Molecular-Genetic Methods and Prospects for Their Application for the Indication and Identification of <i>Yersinia pestis</i> Strains by K. A. Nikiforov

“…The methods are also considered from the perspective of their promising application at three levels (territorial, regional and federal) of the system for laboratory diagnosis of infectious diseases at the premises of Rospotrebnadzor organizations to solve the problem of maintaining the sanitary and epidemiological well-being of the country’s population. The main groups of methods considered are as follows: based on the analysis of the lengths of restriction fragments (ribo- and IS-typing, pulse gel electrophoresis); based on the analysis of specific fragments (DFR typing, VNTR typing); based on sequencing (MLST, CRISPR analysis, SNP analysis); PCR methods (including IPCR, SPA); isothermal amplification methods (LAMP, HDA, RPA, SEA, PCA, SHERLOCK); DNA-microarray; methods using aptamer technology; bio- and nano-sensors; DNA origami; methods based on neural networks. …”
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In Vitro Examination of Fungal and Root Extracts Inspired by Traditional Medicine for Potential Periorbital Eye Infrastructure Treatments by James V. Gruber, Nicole Terpak, Sebastien Massard, Xiang Chen, John Craffey, Robert Holtz

“…In addition, the extracellular matrix that surrounds the eye is exposed to external threats like UV radiation, weather and pollution, as well as lifestyle choices that create fatigue. This causes the eyes to express wrinkles well before they begin to appear on the rest of the face, particularly in the corners of the eyes called the crow’s feet region. …”
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The Prognostic Significance and Co-Expression of Fibroblast Growth Factor Receptor 2 and c-Met in Endometrial Cancer by Gao H, Lu Q, Zhang J

“…Tissue microarray construction, immunohistochemical staining and scoring were employed to study the expression of FGFR2 and c-Met. …”
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FOXO4-DRI induces keloid senescent fibroblast apoptosis by promoting nuclear exclusion of upregulated p53-serine 15 phosphorylation by Yu-Xiang Kong, Zhi-Shuai Li, Yuan-Bo Liu, Bo Pan, Xin Fu, Ran Xiao, Li Yan

“…Here we find increased proportion of pro-inflammatory and mesenchymal fibroblast subpopulations and senescent fibroblasts, and enhanced expression of senescence-associated secretory phenotype genes using single-cell RNA sequencing analysis, as well as elevated p16 protein and more β-galactosidase-positive cells in keloids. …”
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Bioinformatics and network pharmacology-based study to elucidate the multi-target pharmacological mechanism of the indigenous plants of Medina valley in treating HCV-related hepato... by Mubarak A. Alamri

“…Moreover, the molecular docking and Molecular Dynamic (MD) simulation of 20 ns well complemented the binding affinity of the compound and revealed strong stability of predicted compounds at the docked site. …”
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R-locus for roaned coat is associated with a tandem duplication in an intronic region of USH2A in dogs and also contributes to Dalmatian spotting. by Takeshi Kawakami, Meghan K Jensen, Andrea Slavney, Petra E Deane, Ausra Milano, Vandana Raghavan, Brett Ford, Erin T Chu, Aaron J Sams, Adam R Boyko

“…We identified a putative causal variant in this region, an 11-kb tandem duplication (11,131,835-11,143,237) characterized by sequence read coverage and discordant reads of whole-genome sequence data, microarray probe intensity data, and a duplication-specific PCR assay. …”
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Regulation of lifespan, metabolism, and stress responses by the Drosophila SH2B protein, Lnk. by Cathy Slack, Christian Werz, Daniela Wieser, Nazif Alic, Andrea Foley, Hugo Stocker, Dominic J Withers, Janet M Thornton, Ernst Hafen, Linda Partridge

“…Biochemical and genetic data suggest that Lnk functions in both the IIS and Ras/Mitogen activated protein Kinase (MapK) signaling pathways. Microarray studies support this model, showing transcriptional feedback onto genes in both pathways as well as indicating global changes in both lipid and carbohydrate metabolism. …”
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The impact of MITF expression on tumor-infiltrating lymphocytes in melanoma: Insights into immune microenvironment dynamics by Damir Vučinić, Matea Lekić, Gordana Žauhar, Gordana Zamolo

“…Archival formalin-fixed, paraffin-embedded tissue samples from 81 primary melanoma patients were analyzed via tissue microarray immunohistochemistry to assess MITF protein levels. …”
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The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam. by Nhu Thi Khanh Nguyen, Vinh Ha, Nga Vu Thieu Tran, Richard Stabler, Duy Thanh Pham, Thi Minh Vien Le, H Rogier van Doorn, Ana Cerdeño-Tárraga, Nicholas Thomson, James Campbell, Van Minh Hoang Nguyen, Thi Thu Nga Tran, Minh Van Pham, Thuy Thu Cao, Brendan Wren, Jeremy Farrar, Stephen Baker

“…These investigations included PCR amplification for common ESBL genes, plasmid investigation, conjugation, microarray hybridization and DNA sequencing of a bla(CTX-M) encoding plasmid.…”
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The Olive Oil Monophenolic Secoiridoid Ligstroside Aglycone Suppresses Melanoma Progression by Targeting the BRAF Signaling Pathway by Md Ashiq Mahmud, Abu Bakar Siddique, Afsana Tajmim, Judy Ann King, Khalid A. El Sayed

“…A Western blot analysis of the collected animal tumors further validated the downregulation of the mutated BRAF–MAPK axis, as well as the GPD1 and ELOVL6 expression levels. A histopathological analysis of Malme-3M tumor sections showed extensive focal tumor necrosis in treated mice. …”
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A signature of 33 immune‐related gene pairs predicts clinical outcome in hepatocellular carcinoma by Xiao‐Yan Sun, Shi‐Zhe Yu, Hua‐Peng Zhang, Jie Li, Wen‐Zhi Guo, Shui‐Jun Zhang

“…Methods Two RNA‐seq datasets (The Cancer Genome Atlas Program and International Cancer Genome Consortium) and one microarray dataset (GSE14520) were included in this study. …”
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Comparison of effects of p53 null and gain-of-function mutations on salivary tumors in MMTV-Hras transgenic mice. by Dadi Jiang, Catherine I Dumur, H Davis Massey, Viswanathan Ramakrishnan, Mark A Subler, Jolene J Windle

“…Salivary tumors from these mice were characterized with regard to age of tumor onset, tumor growth rates, cell cycle distribution, apoptotic levels, tumor histopathology, as well as response to doxorubicin treatment. Microarray analysis was also performed to profile gene expression. …”
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Promoting Osteogenic Differentiation of Human Adipose-Derived Stem Cells by Altering the Expression of Exosomal miRNA by Shude Yang, Shu Guo, Shuang Tong, Xu Sun

“…Furthermore, in order to investigate the importance of exosomal microRNAs (miRNAs) in osteogenic differentiation of ADSCs, we used microarray assays to analyze the expression profiles of exosomal miRNAs derived from undifferentiated as well as osteogenically differentiated ADSCs; 201 miRNAs were upregulated and 33 miRNAs were downregulated between the two types of exosomes. …”
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Differential BACH1 Expression in Basal-like Breast Tumors of Black Women Identified via Immunohistochemistry by N. M. Dowling, Galina Khramtsova, Olufunmilayo Olopade, Shabnam Samankan, Bok-Soon Lee, Jiyoung Lee

“…Importantly, BACH1 expression was significantly higher in tumors from Black women compared to those from White women, as well as in the basal-like subtype of breast tumors from Black women. …”
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Comparison of In Vivo Gene Expression Profiling of RPE/Choroid following Intravitreal Injection of Dexamethasone and Triamcinolone Acetonide by Zeljka Smit-McBride, Elad Moisseiev, Sara P. Modjtahedi, David G. Telander, Leonard M. Hjelmeland, Lawrence S. Morse

“…Differential gene expression of over 34,000 well-characterized mouse genes in the RPE/choroid of 6-week-old C57BL/6J mice was analyzed after intravitreal steroid injections at 1 week and 1 month postinjection, using Affymetrix Mouse Genome 430 2.0 microarrays. …”
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RETRACTED: EZH2-Mediated microRNA-375 Upregulation Promotes Progression of Breast Cancer via the Inhibition of FOXO1 and the p53 Signaling Pathway by Xin Guan, Aiping Shi, Yabin Zou, Meiyang Sun, Yue Zhan, Yi Dong, Zhimin Fan

“…Differentially expressed genes between tumor and paired normal tissues from BC patients were screened out by microarray analyses. miR-375 was abundantly expressed in BC tissues and cells, and it was correlated with the poor prognosis of patients. …”
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Enhancing Binary Classification Performance in Biomedical Datasets: Regularized ELM with SMOTE and Quantile Transforms Focused on Breast Cancer Analysis by Brilliant Friezka Aina, Meta Kallista, Ig. Prasetya Dwi Wibawa, Ginaldi Ari Nugroho, Ivana Meiska, Syifa Melinda Naf’an

“…Using microarray datasets, this research investigation addresses the problem of unbalanced data in binary classification tasks. …”
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