Modular and efficient assembly of CRISPR vectors and construction of an apple gene editing efficiency evaluation system by HOU Feifan, HUANG Chuanmi, HAO Pengbo, CONG Liu, BAI Tuanhui, SHI Jiangli, WANG Miaomiao, LIU Yu, ZHAO Yujie, WAN Ran, SHEN Yawen, ZHANG Hengtao, ZHANG Kunxi, ZHENG Xianbo, JIAO Jian

“…This platform enables flexible replacement and rapid reconstruction of core components including Cas proteins, promoters, and gRNA expression cassettes, and establishes a system for visual detection of precise plasmid transfection by integrating the RUBY reporter system. …”
Get full text

Early cerebrospinal fluid elevations of pTau-217 in severe traumatic brain injury subjects by Hamad Yadikar, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Claudia Robertson, Spyridoula Tsetsou, John B. Williamson, John B. Williamson, Damon G. Lamb, Damon G. Lamb, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Todd Kibaugh, Shih-Han Kao, Zhifeng Kou, Zhifeng Kou, Robert D. Welch, Jose-Miguel Yamal, Luis Leon-Novelo, Richard Rubenstein, Richard Rubenstein, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang

Get full text

Comprehensive characterization of tobacco-induced changes in enamel surface topography by Tamanna Kaur, Ramya Ramadoss, Nitya Krishnasamy, Sandhya Sundar, Suganya Panneer Selvam, Hema Shree K

“…To assess these changes, various methods such as visual examinations, digital photography, spectrophotometry, stylus profilometry, and atomic force microscopy (AFM) are utilized. …”
Get full text

Basic research on postoperative cognitive dysfunction in the past decade: a bibliometric analysis by Hongwei Wu, Jiannan Song, Jiannan Song, Zhanfei Hu, Zhanfei Hu, Haibo Li, Haibo Li, Qi Zhou, Qi Zhou, Congcong Dai, Congcong Dai, Yi Gao, WanLi Ma, WanLi Ma

“…This study aims to employ bibliometric methods to comprehensively summarize the current status and developmental trends of basic research on POCD, providing new ideas and strategies for future scientific investigations.MethodsRelevant literature published between January 1, 2014, and October 30, 2024, was retrieved from the Web of Science Core Collection. …”
Get full text

Development and validation of a sliding type continuous passive motion automation device for evaluation and rehabilitation of frozen shoulder: a pilot study by Jewoo Lee, Sung-sik Yun, Kyung Rok Oh, Kyung Rok Oh, Sun Gun Chung, Sun Gun Chung, Wonjae Hwang, Wonjae Hwang, Keewon Kim, Keewon Kim, Kyu-jin Cho

“…Current rehabilitation methods, including physical therapy and passive range of motion (PROM) exercise, can be limited by cost and availability. …”
Get full text

A Comprehensive Framework for Parkinson's Disease Detection Using Spiral Drawings and Advanced Machine Learning Techniques by Mohamed J. Saadh, Waleed K. Abdulsahib, Hardik Doshi, Anupam Yadav, J. Gowrishankar, Mayank Kundlas, Nargiza Mansurova, Kamal Kant Joshi, Fadhil Feez Sead, Bagher Farhood

“…It integrates advanced machine learning techniques to improve diagnostic accuracy and practical application in clinical settings. Materials and Methods Spiral drawing data were collected from a comprehensive dataset, including samples from both Parkinson's patients and healthy individuals. …”
Get full text

Personality traits, pain perception, and patient attitudes toward orthodontic treatment with fixed appliances by Monika Lorek, Anna Jarząbek, Magdalena Sycińska-Dziarnowska, Sylwia Gołąb, Ewa Cichocka, Gianrico Spagnuolo, Gianrico Spagnuolo, Krzysztof Woźniak, Liliana Szyszka-Sommerfeld, Liliana Szyszka-Sommerfeld

“…This, in turn, encourages the search for effective methods of pain reduction during orthodontic treatment and attention to communication between orthodontists and patients for a good understanding of the procedures used.…”
Get full text

Development and validation of a nomogram for predicting in-hospital mortality in older adult hip fracture patients with atrial fibrillation: a retrospective study by Zhenli Li, Jing He, Tiezhu Yao, Guang Liu, Jing Liu, Ling Guo, Mengjia Li, Mengjia Li, Zhengkun Guan, Zhengkun Guan, Ruolian Gao, Jingtao Ma

“…Next, 10 features, which were the intersection of features selected by the above methods, were further utilized to develop an XGBoost model to obtain the rank of feature importance. …”
Get full text

Internet of things-based smart system for apple orchards monitoring and management by Bahareh Jamshidi, Hossein Khabbaz Jolfaee, Kazem Mohammadpour, Mohsen Seilsepour, Hossein Dehghanisanij, Hassan Hajnajari, Hossein Farazmand, Alireza Atri

“…Evaluation results indicated that the system based on the determined forecasting methods can reduce the number of spraying times (twice instead of at least three times to control apple powdery mildew, and twice instead of four times to control apple codling moth). …”
Get full text

Deep learning feature-based model for predicting lymphovascular invasion in urothelial carcinoma of bladder using CT images by Bangxin Xiao, Yang Lv, Canjie Peng, Zongjie Wei, Qiao Xv, Fajin Lv, Qing Jiang, Huayun Liu, Feng Li, Yingjie Xv, Quanhao He, Mingzhao Xiao

“…Methods Data and CT images of 577 patients across four medical centers were retrospectively collected. …”
Get full text

A non-linear association of low-density lipoprotein cholesterol with all-cause and cardiovascular mortality among patients with hypertension by Guoliang Liang, Guoliang Liang, Wenhao Zhang, Xinxin Gu, Qiong Zhang, Ankang Liu, Xinran Qing, Jiangwei Ma

“…A restricted cubic spline curve was performed to visually represent the relationship between LDL-C and mortality. …”
Get full text

The corneoscleral shell of the eye: potentials of assessing biomechanical parameters in normal and pathological conditions by E. N. Iomdina, S. Yu. Petrov, A. A. Аntonov, I. A. Novikov, I. A. Pahomova, A. Y. Archakov

“…The paper reviews modern methods of evaluating the biomechanical properties of the corneoscleral shell of the eye that can be used both in the studies of the pathogenesis of various ophthalmic pathologies and in clinical practice. …”
Get full text

Major Salivary Gland Examinations of the Donkey (Equus asinus) Using Sectional Anatomy, Computed Tomography and Magnetic Resonance Imaging by Jamal Nourinezhad, Albert Abdi, Abdolvahed Moarabi, Mohamad Ghasem Hanafi, Rahmat Allah Fatahian Dehkordi, Anna Tomańska

“…ABSTRACT Visualizing the major salivary glands (MSGs) in domestic mammals using plain radiology and identifying damaged their structures is impossible. …”
Get full text

Unmasking the Hidden Morbidity of Ocular Diseases in Primary Care Through a Collaboration with Specialists in Remote Areas: A Cross-Sectional Study from Rural Crete, Greece by Konstantinos Chliveros, Manolis Linardakis, Ioanna Tsiligianni, Miltiadis Tsilimbaris, Ioannis Pallikaris, Christos Lionis

“…The National Eye Institute Visual Function Questionnaire-25 (NEI VFQ-25) was applied to measure self-reported vision-targeted health status. …”
Get full text

Ocular Manifestations in Children with Vernal Keratoconjunctivitis by Matteo Capobianco, Marco Zeppieri, Giuseppe Gagliano, Federico Visalli, Fabiana D’Esposito, Roberta Foti, Ludovica Cannizzaro, Daniele Tognetto, Caterina Gagliano

“…Aims: This review summarizes the main factors to consider when diagnosing, treating, and managing patients with VKC based on the current literature in this field. Methods: This comprehensive review examined peer-reviewed literature from 2010 to 2024 obtained from PubMed. …”
Get full text

Optimal proprioceptive training combined with rehabilitation regimen for lower limb dysfunction in stroke patients: a systematic review and network meta-analysis by Kaiqi Zheng, Li Li, Yahui Zhou, Xiaokun Gong, Gangbin Zheng, Liang Guo

“…For walking ability, PT in combination with visual feedback training (PT + VFT) was superior (SUCRA 96.61%). …”
Get full text

Efficacy of adalimumab in pediatric non-infectious uveitis with and without anterior segment inflammation by Chunbo Zhang, Xiaorong Xue, Jinan Xiao, Qiongge Li, Yuyao Zhai, Ying Chen

“…Abstract Background This study aimed to identify the clinical characteristics of cases that is related to the response rate of adalimumab (ADA) treatment. Methods A retrospective review of medical records was conducted for pediatric patients with non-infectious uveitis undergoing ADA treatment for a minimum of six months. …”
Get full text

Associations between folate intake and knee pain, inflammation mediators and comorbid conditions in patients with symptomatic knee osteoarthritis by Zhenhua Liu, Ze Chen, Zewei Wu, Mingze Tang, Yongcong Lin, Cuixi Wu, Zhaohua Zhu, Guangfeng Ruan, Changhai Ding, Weiyu Han

“…Abstract Background To investigate the associations between folate intake and changes in knee pain, inflammation mediators and comorbid conditions over 2 years in patients with symptomatic knee osteoarthritis (OA). Methods A post-hoc analysis was performed based on data from the VIDEO study, a multicenter, randomized, double-blind, placebo-controlled clinical trial aimed at assessing the impact of vitamin D supplementation on patients with knee OA who were also vitamin D deficient. …”
Get full text

Transcutaneous and transcranial electrical stimulation for enhancing military performance: an update and systematic review by Onno van der Groen, Sara A. Rafique, Nick Willmot, Margaret G. Murphy, Margaret G. Murphy, Eulalia Tisnovsky, Tad T. Brunyé, Tad T. Brunyé

“…Various types of ES, such as transcranial direct current stimulation (tDCS) and transcranial alternating current stimulation (tACS), modulate neuronal membrane potentials and cortical excitability, potentially improving cognitive functions relevant to military training and operations.MethodsThis systematic review updates previous findings by examining studies published between 2019 and 2024 that investigated electrical stimulation effects on cognitive performance in military personnel and tasks. …”
Get full text

Regulation of microtubule nucleation in glioblastoma cells by ARF GTPase-activating proteins GIT1 and GIT2 and protein kinase C by Vadym Sulimenko, Eduarda Dráberová, Vladimíra Sládková, Tetyana Sulimenko, Věra Vosecká, Omar Skalli, Pavel Dráber

“…To further investigate their role in microtubule modulation, we examined the function of GITs in microtubule nucleation and the involvement of protein kinase C (PKC) in this process. Methods Glioblastoma cell lines with depleted GIT protein levels were generated using shRNA lentiviral vectors. …”
Get full text