Molecular cloning of EZH2 oncogene in chronic myeloid leukemia and therapeutic prospects by Juliana Costa Gaspar, Lucas Santos de Santana, Camila Menezes Freire de Souza, Marcos Montani Caseiro, Rosane Rezende de Souza Giuliani, Cleide Barbieri de Souza

“…Among the new therapeutic approaches to CML is gene therapy, which, depending on the target gene, can be efficient to other neoplasms treatment. …”
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Cloned airway basal progenitor cells to repair fibrotic lung through re-epithelialization by Yu Zhao, Yueqing Zhou, Weipan Zhang, Mingzhe Liu, Jun Duan, Xiaopeng Zhang, Qiwang Ma, Yujia Wang, Yuzhen Zhang, Zhongliang Guo, Ting Zhang, Wei Zuo

“…Aided with an automated culture system, we clone and characterize airway basal progenitor cells from 44 donors with various lung conditions. …”
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Cloning, expression, and purification of fusion antigens MPT83 and ESAT6 from the local strain of Mycobacterium tuberculosis in Escherichia coli as a seed vaccine candidate against... by Rusdina Bte Ladju, Ahyar Ahmad, Abdul Karim, Rugaiyah Arfah, Rosana Agus, Najdah Hidayah, Muhammad Nasrum Massi, Astutiati Nurhasanah, Harningsih Karim, Irda Handayani

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Identification of novel anti-CD16a antibody clones for the development of effective natural killer cell engagers by Bill Liao, Christine Tumanut, Lin Li, Adam Corper, Dilip Challa, Alex Chang, Hydari Begum, Elinaz Farokhi, Catherine Woods, Xiaomin Fan

“…These anti-CD16a antibody clones are promising candidates for combination with any TAA of interest, offering the potential for novel NK cell engager-based cancer therapeutics that are minimally affected by the high concentrations of human IgG in the circulation.…”
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Efficient Predictor for Immunotherapy Efficacy: Detecting Pan‐Clones Effector Tumor Antigen‐Specific T Cells in Blood by Nanoparticles Loading Whole Tumor Antigens by Weibiao Zeng, Jin Wang, Zhike Chen, Jian Yang, Ao Zhu, Yan Zheng, Xianlan Chen, Yuhan Liu, Leilei Wu, Yufeng Xie, Sheng Ju, Jun Chen, Cheng Ding, Chang Li, Xin Tong, Mi Liu, Jun Zhao

“…ETASTs in patients with lung cancer are higher than those in healthy individuals and those with benign pulmonary nodules. Therapeutic efficacy positively correlated with the number of ETASTs in the blood. …”
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High CXCR3 on Leukemic Cells Distinguishes IgHVmut from IgHVunmut in Chronic Lymphocytic Leukemia: Evidence from CD5high and CD5low Clones by Gayane Manukyan, Tomas Papajik, Zuzana Mikulkova, Renata Urbanova, Veronika Smotkova Kraiczova, Jakub Savara, Milos Kudelka, Peter Turcsanyi, Eva Kriegova

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Core-genome guided novel therapeutic targets identification and chimeric vaccine designing against Rickettsia rickettsii by Fizza Arshad, Asifa Sarfraz, Muhammad Shehroz, Umar Nishan, Asia Perveen, Riaz Ullah, Mohamed A. Ibrahim, Mohibullah Shah

“…Moreover, the identified drug targets were annotated for essential biological processes, which shed light on their therapeutic potential. The vaccine constructs were cloned and expressed in suitable systems. …”
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Target-specific peptides for BK virus agnoprotein identified through phage display screening: advancing antiviral therapeutics by Xiaofei Xing, Jingxian Han, Keke Wang, Fuyun Tian, CuiXia Jiang, Wei Liang, Lin Qi, Xin Yue, Yinhang Wen, Yuwei Hu, Hui Qiao

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Stromal-Cell-Derived Factor-1 (SDF-1)/CXCL12 as Potential Target of Therapeutic Angiogenesis in Critical Leg Ischaemia by Teik K. Ho, X. Shiwen, D. Abraham, J. Tsui, D. Baker

“…This review will focus on the role of the SDF-1/CXCR4 system in the pathophysiology of PVD and discuss their potential as therapeutic targets for PVD.…”
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Acinetobacter baumannii infection in critically ill patients with COVID-19 from Tehran, Iran: the prevalence, antimicrobial resistance patterns and molecular characteristics of iso... by Mahsa Ghamari, Fereshteh Jabalameli, Shirin Afhami, Shahnaz Halimi, Mohammad Emaneini, Reza Beigverdi

“…Genetic fingerprinting using REP-PCR revealed that 39 typeable A. baumannii isolates were categorized into 12 distinct genotypes, of which 72% (28/39) of isolates belonged to one genotype.ConclusionThe high prevalence of XDR A. baumannii such as carbapenem and colistin-resistant strains, poses a significant concern for the treatment of COVID-19 patients, heightening the risk of therapeutic failure. The data demonstrate the dissemination of a single A. baumannii clone carrying multiple ARGs within our hospital. …”
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Development and evaluation of a recombinant monoclonal human antibody with virus-neutralizing activity against the F glycoprotein of respiratory syncytial virus by Sergey A. Klotchenko, Ekaterina A. Romanovskaya-Romanko, Marina A. Plotnikova, Anastasia A. Pulkina, Aram A. Shaldzhyan, Dmitry S. Balabashin, Victoria A. Toporova, Teimur K. Aliev, Natalia E. Gyulikhandanova, Dmitry A. Lioznov

“…The authors are currently developing a stable FM1 producer clone with high productivity and viability and investigating the therapeutic efficacy of this antibody in a sublethal RSV infection mouse model.…”
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Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma by Yujiao Zhou, Feifei Liu, Chengmin Li, Guo Shi, Xiaolei Xu, Xue Luo, Yuanling Zhang, Jingjie Fu, Aizhong Zeng, Limin Chen

“…Aspartate-β-hydroxylase (AAH), an overexpressed tumor-associated cell surface protein, is considered as a promising biomarker and therapeutic target for HCC. In this study, we constructed adenovirus vector encoding AAH gene by gateway recombinant cloning technology and preliminarily explored the antitumor effects of DC vaccines harboring AAH. …”
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Immunoinformatics Approach to Design a Chimeric CD70-Peptide Vaccine against Renal Cell Carcinoma by Haideh Namdari, Farhad Rezaei, Fatemeh Heidarnejad, Mohammad Yaghoubzad-Maleki, Maryam Karamigolbaghi

“…Overall, the results suggest therapeutic potential for the chimeric CD70-CPP-TNF vaccine against RCC. …”
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Molecular dynamics simulation based prediction of T-cell epitopes for the production of effector molecules for liver cancer immunotherapy. by Sidra Zafar, Yuhe Bai, Syed Aun Muhammad, Jinlei Guo, Haris Khurram, Saba Zafar, Iraj Muqaddas, Rehan Sadiq Shaikh, Baogang Bai

“…The current treatments are only effective in early stages of cancer. To overcome the therapeutic challenges and exploration of immunotherapeutic options, broad spectral therapeutic vaccines could have significant impact. …”
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Utilizing the subtractive proteomics approach to design ensemble vaccine against Candida lusitaniae for immune response stimulation; a bioinformatics study. by Habiba Naz, Rimsha Timotheous, Muhammad Farhan Sarwar, Tariq Nadeem, Mudassar Fareed Awan, Sajed Ali, Sophia Awais, Irfan Ahmed

“…Vaccines have always been one of the promising therapeutic sources against many pathogens including infectious fungi. …”
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Phage Therapy -- Everything Old Is New again by Andrew M Kropinski

“…Because of their ability to transmit genes, phages have been exploited to develop cloning vector systems. They also provide a plethora of enzymes for the modern molecular biologist. …”
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A systematic screening assay identifies efficient small guide RNAs for CRISPR activation by Elin Arvidsson, Diana Duarte Lobo, Diana Duarte Lobo, Ermelinda Sabarese, Fabio Duarte, Rui Jorge Nobre, Rui Jorge Nobre, Rui Jorge Nobre, Luis Quintino, Cecilia Lundberg

“…The overall workflow was validated using therapeutically relevant genes for neurodegenerative diseases, including Tfeb, Adam17, and Sirt1. …”
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Recombinant short TNF-BD protein from smallpox virus is pharmacologically active in an experimental septic shock model by I. P. Gileva, S. N. Yakubitskiy, I. V. Kolosova, S. N. Shchelkunov

“…Recombinant VARV-CrmB protein has been produced in insect cells using molecular cloning methods and its TNF neutralizing activity has been shown in vitro and in vivo. …”
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Immortalized Mesenchymal Stem Cells: A Safe Cell Source for Cellular or Cell Membrane-Based Treatment of Glioma by Yuxuan Zhang, Jie Liu, Yunzhao Mo, Zetao Chen, Taoliang Chen, Yan Li, Yaofeng Zheng, Shaokang Deng, Xiangdong Xu, Huajian Chen, Haoqi He, Jiansheng Chen, Tao Jin, Xinlin Sun, Yiquan Ke, Jihui Wang

“…Mesenchymal stem cells (MSCs) have emerged as putative therapeutic tools due to their intrinsic tumor tropism, and anti-tumor and immunoregulatory properties. …”
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Development of a stable eukaryotic strain producing fully human monoclonal antibody on the basis of the human antibody against ectromelia virus by A. L. Matveev, Ya. A. Khlusevich, I. K. Baykov, I. V.  Babkin, E. P. Goncharova, V. V. Morozova, N. V. Tikunova

“…Fully­human antibodies have a great therapeutic importance; however, the development of stable strains providing a high level of production of full­size antibodies is a challenging task, as antibody molecules contain two types of polypeptide chains. …”
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