Protocol for CRISPR-mediated deletion of cis-regulatory element in murine Th17 cells for in vivo assessment of effector function by Xiancai Zhong, Hongmin Wu, Guanpeng Wang, Zuoming Sun

“…Summary: Studying the cis-regulatory elements (CREs) of genes in Th17 cells during autoimmune disease progression, such as experimental autoimmune encephalomyelitis (EAE), is often limited by the availability of gene-edited mice. …”
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Protocol for purification, crystallization, and structure determination of the mpox virus mRNA cap N7 methyltransferase complex by Qingqing Guo, Anke Chen, Jin Ding, Jixi Li

“…Summary: The mpox virus (MPXV) mRNA cap N7 methyltransferase (RNMT) methylates guanosine at mRNA 5′-cap N7 positions to enable immune evasion. …”
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Protocol for assessing regional pathology in the rodent optic nerve using longitudinal cryosections and cross-sectional electron microscopy by Gabrielle M. Mey, John Shelestak, Muhammad Irfan, Tara M. DeSilva

“…Summary: Visual system pathology has been established as a prominent feature in demyelinating diseases such as multiple sclerosis (MS). …”
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Protocol for multiplex immunofluorescence imaging of mouse intestinal tumors by Shuqin Li, Siyuan You, Kairui Mao

“…Summary: The tumor microenvironment (TME) plays a critical role in both tumor progression and therapeutic efficacy. …”
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Protocol for generating protein profiles and distance-based network analysis of murine tissue slices by Luisa Schmidt, Philipp Antczak, Marcus Krüger

“…Summary: We introduce a protocol for spatial proteomics using thin cryotome sections of mouse skeletal muscle tissue. …”
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Protocol for whole-tissue immunolabeling, optical clearing, and lightsheet imaging of c-Fos protein expression in unsectioned mouse brains by Koukou Fu, Jing Yang

“…Summary: The c-Fos protein has been broadly utilized as a marker of neuronal activity, and conventional immunohistochemistry to determine its expression relies on tissue sections. …”
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Protocol for phase-amplitude coupling analysis in local field potentials from macaque monkeys to investigate neural oscillation dynamics by Mohammad Aliramezani, Ashkan Farrokhi, Christos Constantinidis, Mohammad Reza Daliri

“…Summary: Phase-amplitude coupling (PAC) is a critical neural mechanism that reflects the coordination between oscillatory brain activity across different frequency bands. …”
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Protocol for the quantitative assessment of social and nonsocial reward-seeking in mice using an automated two choice operant assay by Jennifer Isaac, Hymavathy Balasubramanian, Sonia Corbett Karkare, Nicholas Schappaugh, Malavika Murugan

“…Summary: The complexity of social behaviors makes it difficult to study the neural mechanisms that underlie them. …”
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Protocol to study phagocytosis of apoptotic murine splenocytes by syngeneic antigen-presenting cells in vitro by Lukas M. Braun, Robert Zeiser

“…Summary: Extracorporeal photopheresis (ECP) effectively reduces immune checkpoint inhibitor (ICI)-induced colitis and is based on the phagocytic uptake of apoptotic ECP-treated splenocytes. …”
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Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model by Shuyi Liu, Wei Si, Zhengbo Wang

“…Summary: The lack of an etiologic Parkinson’s disease (PD) model in non-human primates has inhibited our understanding of the disease and the development of therapies. …”
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Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach by Nadia Krarup Knudsen, Christine Hjorth Andreassen, Martin Blomberg Jensen, Anne Jørgensen

“…Summary: Testicular function relies on tightly regulated cell-cell interactions, including Sertoli-Leydig cell communication and Sertoli cells nurturing germ cells. …”
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Protocol for in vivo lineage tracing of regeneration-associated macrophages from injured skeletal muscle of adult mice by Neuza S. Sousa, Pedro Sousa-Victor, Joana Neves

“…Summary: Macrophages undergo phenotypic transitions that are essential for successful skeletal muscle (SkM) regeneration. …”
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Protocol to study in vivo organ-specific migration of apoptotic splenocytes in mice with tumor and immune checkpoint inhibitor-induced colitis by Lukas M. Braun, Robert Zeiser

“…Summary: Extracorporeal photopheresis (ECP) reduces immune checkpoint inhibitor (ICI)-induced colitis without affecting anti-tumor immunity. …”
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Protocol for cellular age prediction in yeast and human single cells using transfer learning by Subhadeep Duari, Vishakha Gautam, Gaurav Ahuja

“…Summary: Here, we present a protocol for predicting cellular age via computer vision analysis of cellular morphology and aging-related bioactivities from phase contrast microscopy images. …”
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A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types by Lakshmi Jayaram, Ramith Ramu, Deepthi Puttegowda, Jayanthi M K, Chandana Kumari V B, Khang Wen Goh

“…Summary: Lactic acid bacteria (LAB) are a large group produced during carbohydrate fermentation, resulting in lactic acid. …”
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4D light sheet imaging, computational reconstruction, and cell tracking in mouse embryos by Martin H. Dominguez, Jonathon M. Muncie-Vasic, Benoit G. Bruneau

“…Summary: As light sheet fluorescence microscopy (LSFM) becomes widely available, reconstruction of time-lapse imaging will further our understanding of complex biological processes at cellular resolution. …”
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Protocol to rapidly screen CRISPR-Cas9 gene editing outcomes in a cell population by mutating eGFP to a blue or non-fluorescent phenotype by Danny Wilbie, Enrico Mastrobattista, Olivier Gerrit de Jong

“…Summary: When designing genome editing therapy, it is crucial to measure outcomes of DNA damage repair. …”
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Protocol for high-resolution optical mapping of ex vivo rat heart for electrophysiological studies by Yen Ling Sung, Yuan Si Tsai, Chu Ling Chang, Yu Feng Hu

“…Summary: Cardiac optical mapping provides high-resolution spatiotemporal insights into cardiac electrophysiology. …”
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Protocol to study the genomic profile of histone lactylation with CUT&RUN assay in tumor-associated macrophages by Alessandra De Leo, Alessio Ugolini, Filippo Veglia

“…Summary: Lysine lactylation is a distinctive histone modification that plays a crucial role in epigenetic regulation and gene transcription. …”
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Protocol to quantify energy landscape of high-dimensional oscillatory systems using diffusion decomposition by Shirui Bian, Ruisong Zhou, Wei Lin, Chunhe Li

“…Summary: Here, we present a numerical framework to quantify the energy landscape of high-dimensional stochastic oscillatory systems, based on the diffusion decomposition of Gaussian approximation (DDGA). …”
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