Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis by Na-Na Li, Hui Quan, Jun-Bo Luan

“…Summary: Bacteriocytes are specialized insect cells adapted to harbor symbionts. …”
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Protocol to screen chemicals to enhance homology-directed repair in CRISPR-Cas9 gene editing by Min Ju Jo, Hye Jin Nam

“…Summary: CRISPR-Cas9-based gene editing via homology-directed repair (HDR) enables precise modifications, though its efficiency is limited by the prevalence of non-homologous end joining (NHEJ). …”
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Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice by Jiho Song, Tianqu Zhai, Janggun Jo, Jae Won Chang

“…Summary: Activatable photoacoustic imaging probes offer a strategy to efficiently reduce background noise from endogenous chromophores. …”
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Protocol for the imputation of stimulus-induced single-cell gene expression trajectories from time-series scRNA-seq data by Katherine M. Sheu, Alexander Hoffmann

“…Summary: Single-cell RNA sequencing (scRNA-seq) measures cell-to-cell heterogeneous mRNA abundance but destroys the cell and precludes tracking of heterogeneous gene expression trajectories. …”
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Protocol for click labeling of HIV-1 envelope on amber-free virions prepared using genetic code expansion by Wang Xu, Narendra Kumar Gonepudi, Maolin Lu

“…Summary: Site-specific fluorescent labeling of HIV-1 proteins enables direct visualization of their dynamics and trafficking. …”
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Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity by Anjana Nityanandam, Mary H. Patton, Ildar T. Bayazitov, Kyle D. Newman, Kristen T. Thomas, Stanislav S. Zakharenko

“…Summary: Human induced pluripotent stem cells (hiPSCs) can be used to generate assembloids that recreate thalamocortical circuitry displaying short-term and long-term synaptic plasticity. …”
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Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains by Kira Sofie Baur, Barbara Rühle, Tabea Reith, Anica Krieg, Frank Robert Bengelsdorf

“…Summary: We present a protocol to produce electrocompetent Acetobacterium woodii DSM 1030 and Eubacterium callanderi DSM 3468 cells. …”
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Protocol for fabricating elastomeric stencils for patterned stem cell differentiation by Stefanie Lehr, Jack Merrin, Monika Kulig, Thomas George Minchington, Anna Kicheva

“…Summary: Geometrically controlled stem cell differentiation promotes reproducible pattern formation. …”
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The Role of Artificial Intelligence in Personalized Medicine: A Computer Science Perspective by Raul Mihai RADULESCU

“…The investigation defines an entire framework that outlines steps from gathering and preparing the data to the training, validation, and execution of the model, thus illustrating how predictive models can indeed be employed to suggest personalized therapies. …”
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Protocol for making forecasts exogenous in the International Futures model by José Solórzano, Barry B. Hughes, Mohammod T. Irfan, Yutang Xiong, Stellah Kwasi, Abdelrahman Ibrahim, Solikhakhon Makhmatova, Taylor Hanna, Jonathan D. Moyer

“…Summary: Understanding future dynamics and trends in human and natural development is foundational for creating appropriate policy strategies to address planetary and development challenges. …”
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Protocol for single-cell RNA sequencing and spatial transcriptomics of bladder Ewing sarcoma by Weipu Mao, Houliang Zhang, Jie Ji, Jianpung Wu, Ming Chen, Chao Sun

“…Summary: Single-cell RNA sequencing provides a deep understanding of gene expression patterns at the single-cell level, enabling researchers to study cellular heterogeneity and differentiation. …”
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Protocol for encapsulating ticks to study tick hematophagy and tick-virus-host interactions by Eliane Esteves, Clemence Obellianne, Saravanan Thangamani, Meghan E. Hermance

“…Summary: Hard ticks can acquire and transmit various pathogens during their multi-day blood feeding events. …”
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Protocol for non-invasive pulsed-Doppler evaluation of blood flow in the mouse ophthalmic artery by Radwa Awad, Emil Martin, Anilkumar K. Reddy, Iraida Sharina

“…Summary: The number of reliable and non-invasive techniques to characterize ocular blood flow is limited. …”
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Protocol to monitor activation of a two-component system in Dickeya dadantii during chicory leaf infection using Phos-tag gel by Edwige Madec, Jean-Marie Lacroix, Sébastien Bontemps-Gallo

“…Summary: Quantifying the phosphorylation levels of proteins involved in bacterial signaling pathways is essential for understanding their role in stress adaptation and infection processes. …”
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Protocol for the visualization of pRps6-positive cells in larval zebrafish brains using whole-mount immunofluorescence and light-sheet microscopy by Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska

“…Summary: Due to their small size and transparency, larval zebrafish are a useful model for whole-brain imaging. …”
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Protocol to study how expectations guide predictive eye movements and information sampling in humans by Annika Garlichs, Mark Lustig, Matthias Gamer, Helen Blank

“…Summary: Investigating eye movements provides a unique tool to explore how expectations influence information sampling in the visual domain. …”
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Protocol for quantification of circulating immature granulocytes in patients with a chronic myeloid malignancy using flow cytometry by Margot Morabito, Eric Solary, Dorothée Selimoglu-Buet

“…Summary: Immature granulocytes (iGRANs) circulating in the peripheral blood of patients with a chronic myeloid malignancy, such as chronic myelomonocytic leukemia, are immunosuppressive cells that promote disease progression. …”
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Protocol for measuring beta-secretase activity in cell and tissue lysate using a fluorogenic peptide substrate in a 96-well format by Ahmad Mohammad, Emily N. Copeland, Val A. Fajardo, Rebecca E.K. MacPherson

“…Summary: The protease β-secretase (BACE1) plays a crucial role in the formation of amyloid-beta peptides. …”
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Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry by Coralie Berthoux, Pablo E. Castillo, Kaoutsar Nasrallah

“…Summary: Genetically encoded fluorescent sensors are powerful tools for tracking real-time neuromodulator dynamics. …”
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Protocol for the colocalization of yeast peroxisomal membrane proteins and their binding partners using stimulated emission depletion microscopy by Frank N. Mol, Eline M.F. de Lange, Ida J. van der Klei, Rifka Vlijm

“…Summary: Peroxisomes are highly dynamic organelles that play crucial roles in cellular metabolism. …”
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