Investigation of Knowledge, Attitudes and Practices Related to Incontinence-Associated Dermatitis Among Nursing Home Nurses in Shanghai by Yang X, Yang Y, Wang J

“…IAD-related practice scores differed significantly by ostomy wound-related incontinence group membership status (P< 0.05).Conclusion: Nursing home nurses have positive attitudes towards IAD, but their understanding of IAD is not comprehensive, and their execution in practice is insufficient. The results of this study indicate potential directions for future training.Plain language summary: A self-designed questionnaire was used to investigate IAD-related KAPs among 382 nurses from ten nursing homes in Shanghai. …”
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Protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissue by Maria Lamprou, Ana Krotenberg Garcia, Saskia Jacoba Elisabeth Suijkerbuijk

“…Summary: Cell competition is a quality control mechanism that promotes elimination of suboptimal cells relative to fitter neighbors. …”
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Whole-cell patch clamp and extracellular electrophysiology recordings in mouse brain slices by Huimin Chen, Xiangyi Shi, Fuzheng Guo

“…Summary: Electrophysiological recordings enable the assessment of neuronal excitability, synaptic function, and network activity. …”
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Protocol to analyze deep-learning-predicted functional scores for noncoding de novo variants and their correlation with complex brain traits by Enrique Mondragon-Estrada, Sarah U. Morton

“…Summary: Functional impact of noncoding variants can be predicted using computational approaches. …”
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Protocols for monitoring condensate formation and dynamics between the phase-separating proteins SET/TAF-Iβ and cytochrome c by Miguel Á. Casado-Combreras, Miguel A. De la Rosa, Irene Díaz-Moreno

“…Summary: The targeting of several nuclear stress-response factors by translocated cytochrome c upon genotoxic stress has been demonstrated in recent years to involve liquid-liquid phase separation. …”
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Protocol for analyzing T cell and macrophage motility in a mouse pancreatic cancer spheroid model using light-sheet microscopy by Vanessa Zimmer, Benedikt Slusny, Katrin Roth, Christian Bauer

“…Summary: The interaction of macrophages and T cells is pivotal for shaping the immune response to cancer. …”
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Protocol for assessing mitochondrial cholesterol transport and protein molten globule state in steroidogenic and nonsteroidogenic systems by Himangshu S. Bose, Mahuya Bose, Randy M. Whittal

“…Summary: Steroid hormones are essential for the survival of all mammals for carbohydrate metabolism, stress management, and sexual reproduction. …”
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Protocol for achieving enhanced snRNA-seq data quality using Quality Clustering by Paavo J. Tavi, Johannes Ojanen, Pia Laitinen, Suvi Linna-Kuosmanen

“…Summary: Single-nucleus RNA sequencing (snRNA-seq) data analysis presents a challenge in samples that have high levels of ambient RNA contamination. …”
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Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation by Zhengyao Shao, Hong Qiao

“…Summary: Epigenetic modifications play key roles in regulating transcription by altering chromatin structure and accessibility in response to internal cues or external stimuli. …”
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Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvae by Marek B. Körner, Michal Mitro, Dmitrij Ljaschenko, Nicole Scholz

“…Summary: Chordotonal organs are sensory structures containing ciliated neurons specialized in detecting mechanical forces. …”
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Protocol for assessment of CRISPR base editors and their components in Escherichia coli by Rahul Mahadev Shelake, Jae-Yean Kim

“…Summary: Base editing (BE) is a CRISPR technique that allows precise nucleobase conversions. …”
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Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes by Hannah Arnold, Virginia Panara, Amin Allalou, Katarzyna Koltowska

“…Summary: Characterization of zebrafish mutants is routinely time consuming as morphological phenotypes can be subtle or variable, thus hampering robust quantification. …”
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Protocol to study juxtacrine signaling in human vascular cells using a fluorescence labeling co-culture approach by Jose L. Herrera, Masanobu Komatsu

“…Summary: Endothelial cells interact closely with adjacent endothelial cells and pericytes. …”
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Protocol to identify regulatory modules in Parkinson’s disease progression using miRNA data and Boolean modeling by Ahmed Abdelmonem Hemedan, Venkata Satagopam, Reinhard Schneider, Marek Ostaszewski

“…Summary: Regulatory modules are molecules that interact functionally, driving disease processes. …”
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Protocol for mapping RNA G-quadruplex for chromatin-bound RNA using d-rG4-seq by Yong Woo Lee, Vered Levy, Jeannie T. Lee

“…Summary: Here, we present a protocol for using d-rG4-seq, a technique for mapping RNA G-quadruplex (rG4) for chromatin-bound RNA. …”
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Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique by Alexander Brückner, Adrian Brandtner, Sarah Rieck, Bernd K. Fleischmann, Daniela Wenzel

“…Summary: Endothelial cells (ECs) play a major role in vascular homeostasis and pathology. …”
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Protocol for the visualization of bacteria in the tick gut using whole-mount fluorescence in situ hybridization by Adnan Hodžić, Martin Kunert, David Berry

“…Summary: The tick gut harbors a relatively diverse microbial community that includes commensal, beneficial, and pathogenic bacterial species. …”
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Protocol for generating human craniofacial cartilage organoids from stem-cell-derived neural crest cells by Nagashree Avabhrath, Lauren Foltz, Mark Grimes

“…Summary: Here, we present a protocol to generate craniofacial cartilage organoids from human stem cells via neural crest stem cells (NCSCs). …”
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Protocol for closed-loop optogenetic manipulation of cortical activity following hippocampal sharp-wave ripples in freely behaving rats by Hanna den Bakker, Jyh-Jang Sun, Marine Guyot, Fabian Kloosterman

“…Summary: Temporally and spatially selective closed-loop perturbation of brain activity can be used to reveal the contribution of spontaneous neural events to behavior. …”
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Protocol for genetic load analysis in caribou using a modified genomic evolutionary rate profiling by Rebecca S. Taylor, Micheline Manseau, Peng Liu, Paul J. Wilson

“…Summary: Here, we present a protocol to analyze genetic load in caribou (Rangifer tarandus) using a modified version of the genomic evolutionary rate profiling (GERP) program. …”
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