Protocol for assessing T cell receptor-mediated human T cell cytotoxicity by Kathryne E. Marks, Ifeoluwakiisi Adejoorin, John Sowerby, Deepak A. Rao

“…Summary: Measuring the cytotoxicity of human T cells is a valuable but challenging task. …”
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Protocol to study internalization and localization dynamics of exogenously added proteins in detergent-permeabilized human cells by Sritama Ray, Kamalika Mukherjee, Suvendra N. Bhattacharyya

“…Summary: Cell-permeabilization and resealing assays are essential tools for studying the localization and functional roles of exogenous factors in detergent-permeabilized cells. …”
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Protocol for induction, maintenance, and exit from embryo dormancy in mice by Rui Chen, Rui Fan, Ivan Bedzhov

“…Summary: Embryonic dormancy (diapause) is a reproductive adaptation that allows some mammalian species to prolong pregnancy and delay birth by temporarily suspending embryonic development just before implantation. …”
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Protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system by Jinzhi Li, He Zhang, Jianming Gao

“…Summary: A non-invasive system can measure nutrient uptake and photosynthate partitioning simultaneously while allowing organisms to metabolize without interference in the process of measurement. …”
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Protocol for identifying Mycobacterium tuberculosis infection status through airway microbiome profiling by Geoffrey Olweny, Moses Levi Ntayi, Edward Kyalo, Alex Kayongo

“…Summary: This protocol describes the steps to determine an airway microbiome signature for identifying Mycobacterium tuberculosis infection status. …”
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Protocol for lateral patterning of van der Waals heterostructures using sequential chemical vapor deposition by Soonyoung Cha, Hyeuk-Jin Han, Ji-Hoon Ahn, Gangtae Jin

“…Summary: Previous work demonstrates that scalable area-selective deposition of van der Waals monolayers enables tunable design of atomically thin electronic and photonic platforms. …”
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Protocol for capturing a full transcriptome from single preimplantation embryos using So-Smart-seq by Chunyao Wei, Jeannie T. Lee

“…Summary: Strand-optimized Smart-seq (So-Smart-seq) can capture a comprehensive transcriptome from low-input samples. …”
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Protocol for extracting and evaluating activity distributions in rat electrocorticograms with principal component analysis and k-nearest neighbor by Astrid Mellbin, Udaya Rongala, Fredrik Bengtsson

“…Summary: Principal component analysis (PCA) and k-nearest neighbor (kNN) can be applied to extract and compare activity distributions from electrocorticogram (ECoG) signals across recorded neural activity. …”
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Protocol to isolate stress granules in HeLa cells using fluorescence-activated non-membrane condensate isolation by Yilong Zhou, Maria Shvedunova, Asifa Akhtar

“…Summary: Various stress stimuli induce cytosolic stress granules (SGs) in mammalian cells, which are composed of RNA and RNA-binding proteins and have important physiological functions. …”
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Protocol for generating NGN2 iPSC lines and large-scale human neuron production by Xiwei Shan, Amber L. Cramer, Claire G. Jeong

“…Summary: Large-scale production of induced pluripotent stem cell (iPSC)-derived neurons is valuable in disease modeling and drug discovery. …”
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DOPUSZCZALNOŚĆ SKARGI KASACYJNEJ ZE WZGLĘDU NA PRZEDMIOT ZASKARŻENIA by Agnieszka Kincbok

“… Admissibility of Cassation Complaint Subject to the Matter of Appeal Summary The cassation complaint is an appeal from sentences or decisions of courts of the second instance. …”
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Protocol for enhancing RNA yield and quality from single nuclei isolated from mouse brain tissue by Arif Murat Kocabas, Isaac Marin-Valencia

“…Summary: Isolating RNA from single nuclei is essential for single-cell gene expression analysis, yet obtaining high-quality RNA is challenging. …”
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Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model by Ye Liu, George L. Sen

“…Summary: When skin is exposed to ultraviolet B (UVB) radiation, damaged keratinocytes release double-stranded RNA to trigger an inflammatory response. …”
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Protocol for 4D live fluorescence microscopy to image rapid cellular responses in yeast by Yaneris M. Alvarado-Cartagena, Christopher Huppenbauer, Nava Segev

“…Summary: Micro-autophagy is thought to occur through cargo engulfment by the lysosomal membrane based on electron microscopy of fixed cells. …”
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Protocol to identify genes important for Candida albicans fitness in diverse environmental conditions using pooled bar-seq screening approach by Emily H. Xiong, Xiang Zhang, Nicole Robbins, Chad L. Myers, Leah E. Cowen

“…Summary: Identifying genes important for fitness in Candida albicans advances our understanding of this important pathogen of humans. …”
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Protocol for conditional mutagenesis in zebrafish germ cells using Tol2 transposon and a CRISPR-Cas9-based plasmid system by Yixuan Hu, Yutao Chen, Yu Zhang, Zhiquan Liu, Jianzhen Li

“…Summary: Here, we present a protocol for conditional mutagenesis in zebrafish germ cells using Tol2 transposon and a CRISPR-Cas9-based plasmid system. …”
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Theology as a Science by Gerhard Ludwig Müller

“…Theology is not a science in the full extent of the concept, as it is executed by reason, and through this, it participates in the cognition of all reality. …”
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A mixed methods exploration of motor imagery in autistic and non-autistic adults: Diverse experiences and implications for interventions. by Molly Brillinger, Ying Bai, April Karlinsky, Timothy N Welsh, Ellen Poliakoff, Emma Gowen

“…Research on motor imagery (MI) in non-autistic individuals suggests that there are shared neural circuitries between imagery and execution. The relationship between imagined and executed movements, and the use of MI in autistic adults is poorly understood. …”
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Protocol to measure the impact of Clostridioides difficile toxins on antibody responses using ELISA, ELISPOT, and toxin-neutralization assays by Kaylee M. Norman, Gillian A. Lang, Jimmy D. Ballard, Mark L. Lang

“…Summary: To determine the effects of bacterial toxins on the humoral immune response, accurate measurement of antibody quantity, specificity, and function is critical. …”
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Protocol to study DNA strand breaks during development and apoptosis using in situ nick translation in Drosophila by Deepak Maurya, Bama Charan Mondal

“…Summary: Cellular stress causes DNA strand breaks that are typically repaired to maintain homeostasis and regulate cell fate. …”
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