Protocol to assess engulfment and degradation of synaptosomes by murine microglia in vitro by Alessandro Matera, Anne-Claire Compagnion, Rosa Chiara Paolicelli

“…Summary: Microglia, the innate immune cells of the central nervous system, refine neuronal circuitries both during brain development and in neurodegenerative diseases. …”
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Protocol for determining regulators of competence in regeneration of adventitious roots using hypocotyl wounding approach in Arabidopsis by Suresh Damodaran, Lucia C. Strader

“…Summary: Plant plasticity enables the regeneration of adventitious roots upon wounding. …”
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Protocol to identify proteins as regulators of gene expression noise by Óscar García-Blay, Maike M.K. Hansen

“…Summary: Noise regulatory proteins are key to understanding the dynamic regulation of cell-to-cell heterogeneity in gene expression. …”
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Protocol for odorant-binding protein 2A gene knockdown by dual siRNA transfection in a three-dimensional human epidermal equivalent model by Shinobu Nakanishi

“…Summary: Gene knockdown by small interfering RNA (siRNA) transfection is widely used to investigate gene function. …”
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Protocol for synthesizing both racemic and chiral allenylsilanes through copper-catalyzed silylation of propargyl dichlorides by Ying Zhou, Zhengli Liu

“…Summary: Chiral allenylsilanes serve as crucial intermediates in the synthesis of complex pharmaceutical molecules and natural products. …”
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Protocol for identifying key genes using network-based approach as an alternative to differential expression analysis by Piyush Agrawal, Sridhar Hannenhalli

“…Summary: In a variety of biological contexts, characterizing genes associated with disease etiology and mediating global transcriptomic change is a key initial step. …”
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Protocol for predicting suppressors of cell-death pathways based on transcriptomic and vulnerability data by Yaron Vinik, Avi Maimon, Sima Lev

“…Summary: Here, we present a computational protocol for predicting repressors of cancer cell death by combining vulnerability and transcriptomic responses to cell death inducers. …”
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Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice by Kyohei Tokizane, Shin-ichiro Imai

“…Summary: Here, we present a protocol for assessing the impact of a chemogenetic manipulation in a subpopulation of the hypothalamic neurons on aging and lifespan control using a mouse model developed specifically for this purpose. …”
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Protocols for translocation processes of flexible polymers through a pore using LAMMPS by Vrinda Garg, Rejoy Mathew, Arindam Chatterjee, Surya K. Ghosh

“…Summary: Large-scale Atomic/Molecular Massively Parallel Simulator (LAMMPS) is an open-source, powerful simulator with a customizable platform for extensive Langevin dynamics simulations. …”
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Protocol for preparing cellulose-based aerogels in a deep eutectic solvent as surface-enhanced Raman scattering substrates by Kaori Sánchez-Carrillo, Sandeep Surendra Panikar, Josué D. Mota-Morales

“…Summary: There is an increasing need to detect and identify analytes of diverse origins in low molar concentrations and from complex matrices. …”
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Protocol for assessing mobilization of peritoneal B cells to the pre-metastatic omentum in an orthotopic mouse model of ovarian cancer by WonJae Lee, Hironari Akasaka, Honami Naora

“…Summary: The omentum is a visceral adipose tissue that undergoes dynamic immunological changes prior to and following metastasis. …”
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Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus by Lillian I.J. Byer, Huixin Xu, Maria K. Lehtinen

“…Summary: Extracting the choroid plexus (ChP) for histology provides a comprehensive view of the whole tissue and is crucial for understanding its structure and cellular interactions. …”
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Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice by Jiameng Sun, Brian J. DeBosch

“…Summary: To investigate the role of hepatocyte genes in fasting metabolism, it is essential to analyze their expression across the entire 24-h circadian cycle. …”
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Protocol for differential analysis of pseudouridine modifications using nanopore DRS and unmodified transcriptome control by Oleksandra Fanari, Michele Meseonznik, Dylan Bloch, Sara H. Rouhanifard

“…Summary: In this protocol, we detect pseudouridine using nanopore direct RNA sequencing (DRS 002/004) and analyze dynamic changes to mRNA modifications in response to perturbation. …”
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Protocol to isolate dorsal root ganglion neurons from embryonic rats by immunopanning and characterize them using RNAscope and immunofluorescence by Samuel Faraguna, Karina A. Peña, Husniye Kantarci

“…Summary: Dorsal root ganglion (DRG) neurons innervate the periphery of the body to transmit somatosensory information, including touch, pain, and temperature. …”
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Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes by Gaëlle Letort, Elvira Nikalayevich

“…Summary: Mouse oocytes devoid of branched actin display prolonged cortical contractions during the first meiotic division. …”
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Novel Split Latissimus Dorsi Fasciocutaneous Flap With Vascularized Lymph Node Transfer by Fiona R. Fragomen, BS, Julia Maxey, DO, James R. Gatherwright, MD

“…We highlight incorporating VLNT with s-LDF in a 1-stage execution to address both the soft tissue defect and lymphedema. …”
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Forgetting Pronatalism? Abortion Governance and Pro-life Discourses in Post-communist Romania by Lorena Anton

“…The first day after Ceaușescu’s execution, the new government (re)legalized elective abortion. …”
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Protocol for microinjection of rapamycin into the zebrafish habenula by Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska

“…Summary: Mechanistic target of rapamycin complex 1 (mTorC1) activity plays a crucial role in brain development. …”
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Protocol to quantify site-specific carbohydrate sulfation at immune checkpoint proteins in mammalian cells by Wen-Cheng Chou, Wei-Ting Chen, Yuh-Shan Jou, Chen-Yang Shen

“…Summary: Carbohydrate sulfotransferase 8 (CHST8) promotes immune checkpoint activation by sulfating GalNAcβ1-4GlcNAc (LacdiNAc) termini on glycoproteins. …”
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