Protocol to perform polysome profiling in primary differentiating murine adipocytes by Mirian Krystel De Siqueira, Zaynab Nouhi, Yutian Zhao, Siqi Wang, Xinshu Xiao, Xia Yang, Laura Hulea, Claudio J. Villanueva

“…Summary: Here, we present a protocol for polysome profiling in differentiating adipocytes from the mouse stromal vascular fraction. …”
Get full text

Protocol for embedded 3D bioprinting of arterial models with customized contractile and metabolic functions by Huilong Du, Shuyuan Yu, Ziqi Gao, Yuxuan Wang, Huayong Yang, Hongzhao Zhou, Qi Li

“…Summary: 3D bioprinting enables the fabrication of complex tissue structures in vitro. …”
Get full text

Protocol for in vitro phase separation of N terminus of CIDEC proteins by Yiming Lu, Zanzan Zhu, Jianqin Wang, Xuchao Lyu, Peng Li, Feng-Jung Chen

“…Summary: Cell death-inducing DFF45-like effector C (CIDEC) condensation through N-terminal phase separation is a prerequisite for CIDEC-mediated lipid droplet (LD) fusion and lipid storage promotion. …”
Get full text

Protocol for the isolation and characterization of mouse alveolar bone marrow hematopoietic stem cells by Kouta Niizuma, Satoru Morikawa, Eric Gars, Alyssa H. Chang, Adam C. Wilkinson, Hiromitsu Nakauchi, Ryo Yamamoto

“…Summary: Here, we present a protocol for isolating and characterizing hematopoietic stem cells (HSCs) from mouse alveolar bone marrow. …”
Get full text

Protocol for the differentiation of human alveolar epithelial type I cells from pluripotent stem cell-derived type II-like cells by Claire L. Burgess, Lauren J. Ayers, Kasey Minakin, Konstantinos-Dionysios Alysandratos, Xaralabos Varelas, Darrell N. Kotton

“…Summary: Pulmonary alveolar epithelial type I (AT1) cells have a flattened morphology to permit the diffusion of oxygen into the capillaries and historically have been difficult to isolate or maintain in culture. …”
Get full text

Protocol for the establishment of murine orthotopic lung tumors and the assessment of contralateral pulmonal metastasis by flow cytometry by Gregor Zaun, Siyang Liu, Maximilian Webendörfer, Abubakar Wani, Diego Rodriguez, Douglas R. Green, Halime Kalkavan

“…Summary: Murine orthotopic tumor models can accurately resemble cancer biology characteristics including metastasis, drug sensitivity, and remodeling of the tumor microenvironment. …”
Get full text

Protocol for the isolation and characterization of murine hematopoietic stem and progenitor cell-derived extracellular vesicles by Federica Zanotti, Ilaria Zanolla, Massimo Bonora, Claudia Morganti, Barbara Zavan, Letizia Ferroni, Paolo Pinton, Keisuke Ito

“…Summary: Hematopoietic stem cells (HSCs) maintain their self-renewal capacity in an autocrine manner through hematopoietic stem and progenitor cell (HSPC)-derived extracellular vesicles (EVs). …”
Get full text

Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons by Leonardo A. Parra-Rivas, Rohan Sharma, Trinity E. Rust, Hannah O. Bazick, Jared Carlson-Stevermer, Mark J. Zylka, Yuki Ogawa, Subhojit Roy

“…Summary: CRISPR-Cas9 technology enables acute gene knockdown and endogenous tagging to study single-synapse function. …”
Get full text

Protocol for assessing regulatory elements in murine heart using an AAV9-based massively parallel reporter assay by Kaiyu Wang, Meng Meng, Minghong Leng, Yanqing Wang, Jie Ni, Haiyun Yuan, Xiaoran Zhang, Yangpo Cao

“…Summary: The mammalian genome contains thousands of regulatory elements whose activities are highly sensitive to external and internal stimulation. …”
Get full text

Protocol for detecting and analyzing non-oscillatory traveling waves from high-spatiotemporal-resolution human electrophysiological recordings by Veronica M. Zarr, Jyun-You Liou, Edward M. Merricks, Tyler S. Davis, Kyle Thomson, Bradley Greger, Paul A. House, Ronald G. Emerson, Robert R. Goodman, Guy M. McKhann, II, Sameer A. Sheth, Catherine A. Schevon, John D. Rolston, Elliot H. Smith

“…Summary: Innovations in electrophysiological recordings and computational analytic techniques enable high-resolution analysis of neural traveling waves. …”
Get full text

Protocol for the purification, analysis, and handling of acyl carrier proteins from type I fatty acid and polyketide synthases by Christian Gusenda, Svenja Berlage, Ines Burkhart, Benjamin Chagot, Kira J. Weissman, Harald Schwalbe, Martin Grininger

“…Summary: Acyl carrier proteins (ACPs) are key domains in fatty acid and polyketide synthases (PKSs), shuttling intermediates to catalytic partners. …”
Get full text

Protocol for the in vivo tracing of alveolar type I cells from mice using two distinct dual recombinase-mediated genetic approaches by Xueying Yang, Xinfeng Meng, Jingting Zhu, Zhongxiao Wang, Bin Zhou, Kuo Liu

“…Summary: Revealing the origins of alveolar epithelial stem cells is crucial for preventing and treating lung diseases. …”
Get full text

Nicaraguan Migrants in Costa Rica by Catherine M. Marquette

“…This document is the executive summary of a detailed document entitled, Nicaraguan Migrants and Poverty in Costa Rica, which was prepared for the World Bank in 2006. …”
Get full text

Nicaraguan Migrants in Costa Rica by Marquette, Catherine M.

“…This document is the executive summary of a detailed document entitled, Nicaraguan Migrants and Poverty in Costa Rica, which was prepared for the World Bank in 2006. …”
Get full text

Protocol to analyze marrow B lineage cell dynamics by in vivo three-photon microscopy in intact mouse tibia by Anne Bias, Alexander F. Fiedler, Robert Günther, Ruth Leben, Ingeborg Beckers, Anja E. Hauser, Asylkhan Rakhymzhan, Raluca A. Niesner

“…Summary: Three-photon microscopy (3PM) allows deep-tissue imaging beyond the capabilities of two-photon microscopy (2PM) owing to infrared excitation. …”
Get full text

Protocol for deep-learning-driven cell type label transfer in single-cell RNA sequencing data by Zoe Zabetian, Jesus Gonzalez-Ferrer, Julian Lehrer, Vanessa D. Jonsson, Mircea Teodorescu, David Haussler, Mohammed A. Mostajo-Radji

“…Summary: Here, we present a protocol for using SIMS (scalable, interpretable machine learning for single cell) to transfer cell type labels in single-cell RNA sequencing data. …”
Get full text

Protocol for characterizing craniopharyngioma subtypes and their microenvironments using single-cell RNA sequencing and immunohistochemistry by Takashi Kono, Tatsuma Matsuda, Masanori Fujimoto, Yuki Taki, Ikki Sakuma, Naoko Hashimoto, Yasuhiro Nakamura, Kentaro Horiguchi, Yoshinori Higuchi, Atsushi Onodera, Takashi Miki, Tomoaki Tanaka

“…Summary: Here, we present a comprehensive protocol for analyzing craniopharyngioma subtypes and their tumor microenvironments at single-cell resolution. …”
Get full text

A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells by Erdene Baljinnyam, Laura Grisanti, Shalini Tattari, Chiara Pedicone, Bhavana Shewale, Nicole Dubois, Alison M. Goate, William G. Kerr, Samuele G. Marro

“…Summary: The generation of loss-of-function alleles in human pluripotent stem cells (hPSCs) is used to interrogate gene function and validate reagents; however, identifying clones harboring true loss-of-function alleles remains inefficient. …”
Get full text

Bridging the policy-action gap: a scoping review of climate-resilient malaria control policies in Kenya and Zambia with recommendations for strengthening the WHO framework by Isaac Ntwiga, Samuel Kamau Kang’ethe, Josphat Martin Muchangi, Sheila Lumumba, Jackline Kiarie, Sarah Kogei, Levy Mkandawire

“…Policy documents were also reviewed and included. A summary table capturing the related climate-resilient policies was presented, along with supporting discussions.ResultsOf the 274 articles identified, nineteen articles were included in the review. …”
Get full text

Protocol to study the impact of breast cancer on colonization resistance of mouse microbiota using network node manipulation by Ana Laura Cano-Argüelles, Alejandra Wu-Chuang, Lourdes Mateos-Hernandez, Lianet Abuin-Denis, Apolline Maitre, Janet Avellanet, Arlem García, Dasha Fuentes, Alejandro Cabezas-Cruz

“…Summary: Network analysis is a powerful tool for investigating complex interactions between different microbial taxa within a community. …”
Get full text