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261
Protocol for constructing asymmetric triple-atoms supported on nitrogen-doped carbon nanotubes via atomic layer deposition
Published 2025-09-01“…Summary: The precise construction of highly efficient triple-atom catalysts (TACs) remains a significant challenge. …”
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262
Protocol for dorsal ovariectomy and elastase-induced abdominal aortic aneurysm in rats
Published 2025-09-01“…Summary: Women have increased abdominal aortic aneurysm (AAA) growth rates and a higher rupture risk, yet hormonal effects remain unclear. …”
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263
Protocol for the decellularization of human intestinal tissue from patients with inflammatory bowel disease
Published 2025-06-01“…Summary: Decellularization of the human intestine in patients with inflammatory bowel disease (IBD) provides an experimental platform for the investigation of the bowel extracellular matrix (ECM). …”
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264
Protocol for generating brain metastatic tumor cells through repeated intracardiac injections in mice
Published 2025-03-01“…Summary: Here, we present a protocol for generating the lung cancer cell line, LLC1-BMT5, with highly brain metastatic tropism through multiple rounds of in vivo selection. …”
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265
Protocol to reconstruct an in vitro 3D full-thickness skin equivalent model with collagen I in 6- and 12-well inserts
Published 2025-03-01“…Summary: In vitro 3D full-thickness reconstituted human skin has high physiological relevance due to the presence of differentiation features often lacking in 2D cell cultures. …”
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266
Protocol for directed differentiation of human induced pluripotent stem cells into thyroid follicular epithelial cells
Published 2025-09-01“…Summary: We provide a directed differentiation protocol deriving thyroid follicular cells (TFCs) from human induced pluripotent stem cells (iPSCs) or embryonic stem cells without exogenous transcription factors, recapitulating the sequence of developmental milestones of thyroid development. …”
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267
Protocol to study ductal progenitor-like cells from the adult human pancreas using 3D suspension and methylcellulose-based culture systems
Published 2025-06-01“…Summary: Primary human ductal progenitor-like cells derived from donated pancreas have the potential to serve as a source of therapeutic insulin-producing beta cells for the treatment of diabetes. …”
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268
Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants
Published 2025-09-01“…Summary: Here, we present a protocol for a mouse model for studying mechanisms of post-primary pulmonary tuberculosis (PTB) caused by virulent Mycobacterium tuberculosis (Mtb) using subcutaneous hock infection and lung tissue implantation. …”
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269
Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice
Published 2025-03-01“…Summary: The medial entorhinal cortex (MEC) is crucial for context memory, yet its role in context-induced retrieval of morphine withdrawal memory remains to be investigated. …”
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270
Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure
Published 2025-03-01“…Summary: Contrast transfer function (CTF) estimation is essential to the data processing workflow of cryo-electron tomography (cryoET). …”
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271
Protocol for detection of Oropouche viruses from human serum
Published 2025-06-01“…Summary: Oropouche virus (OROV), an arthropod-borne virus, has reemerged in the Americas. …”
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272
Protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of Chagas disease vectors using fluorescent probes
Published 2025-06-01“…Summary: In insects, the circulating lipoproteins vitellogenin and lipophorin function as yolk protein precursors during oogenesis. …”
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273
Protocol to measure temporal consumption and feeding choices of Drosophila adults using capillaries
Published 2025-09-01“…Summary: Here, we present a high-throughput assay for continuously measuring the consumption and feeding choices of individual Drosophila adults over periods of 30 min to several hours. …”
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274
Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate
Published 2025-06-01“…Summary: Here, we present a protocol for a glycolytic stress test using HYlight, a fluorescent protein-based biosensor that specifically binds fructose 1,6-bisphosphate (FBP), a product of the rate-limiting step of glycolysis. …”
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275
Protocol for in vitro generation of innate lymphoid cells from human embryonic tissues
Published 2025-03-01“…Summary: Deciphering the origins of innate lymphoid cells (ILCs) is critical for a deeper understanding of innate immunity. …”
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276
Protocol for tandem enrichment of ubiquitinated, phosphorylated, and glycosylated peptides with SCASP-PTM
Published 2025-09-01“…Summary: Here, we present a protocol for the tandem enrichment of ubiquitinated, phosphorylated, and glycosylated peptides from one sample using the SCASP-PTM (SDS-cyclodextrin-assisted sample preparation-post-translational modification) approach. …”
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277
Distinct role of primate DLPFC and LIP in hierarchical control of learned saccade sequences
Published 2025-01-01“…Summary: Learned action sequences are suggested to be organized hierarchically, but how the various hierarchical levels are processed by different cortical regions remains largely unknown. …”
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278
Protocol for the biofabrication of immunocompetent tumor-on-chip models from patient solid tumors for assessment of anticancer therapies
Published 2025-09-01“…Summary: We present a protocol to generate immunocompetent 3D tumor-on-chip models from human solid tumors, enabling more accurate therapy response assessment than traditional 2D assays. …”
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279
Public Policy Analysis : an integrated approach /
Published 2018Table of Contents: “…Preface -- Methodology of policy analysis -- The process of policy analysis -- Policy analysis in the policy-making process -- Methods of policy analysis -- Structuring policy problems -- Forecasting expected policy outcomes -- Prescribing preferred policies -- Monitoring observed policy outcomes -- Evaluating policy performance -- Methods of policy communication -- Developing policy arguments -- Communicating policy analysis -- Appendix 1: Policy issue papers -- Appendix 2: Executive summaries -- Appendix 3: Policy memoranda -- Appendix 4: Oral briefings -- Index.…”
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280
Protocol for reconstructing ancestral genomes from present-day samples by applying local ancestry inference
Published 2025-03-01“…Summary: The genome of the most recent common ancestor is generally not available but can greatly facilitate the inference of demographic history and the detection of local adaptations. …”
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