Protocol to profile snATAC-seq datasets and motif enrichment analysis during zebrafish early embryogenesis by Jie Zhou, Xueqian Yang, Xiumei Lin, Kaichen Zhao, Xue Wang, Zhiqiang Dong, Chuanyu Liu, Chang Liu

“…Summary: The scarcity of zebrafish-specific motif databases presents a challenge to the analysis of transcription factor (TF) motif within zebrafish single-cell assay for transposase-accessible chromatin using sequencing (scATAC-seq) data, thus hindering the identification of regulatory elements throughout zebrafish embryonic development. …”
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Protocol for assessing the structural architecture, integrity, and cellular composition of murine bone ex vivo by Jonathan W. Lewis, Kathryn Frost, Georgiana Neag, Holly Adams, Emily Powell, Orla Gallagher, Ilaria Bellantuono, Amy J. Naylor, Helen M. McGettrick

“…Summary: Understanding changes in murine bone is essential to comprehend bone homeostasis and the role of therapeutics in bone diseases. …”
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Protocol for a two-clone system to study signaling interactions among neuronal cells in a pre-clinical Alzheimer’s disease model by Aditi Singh, Prajakta Deshpande, Ankita Sarkar, Catherine Yeates, Anuradha Venkatakrishnan Chimata, Madhuri Kango-Singh, Amit Singh

“…Summary: This protocol outlines a two-clone genetic mosaic approach in Drosophila to study cell signaling using the FLP/FRT (Flippase/Flippase recombination target) and Gal4/UAS (upstream activating sequence)/Gal80 systems. …”
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Protocol to derive postganglionic parasympathetic neurons using human pluripotent stem cells for electrophysiological and functional assessment by Christina James, Hsueh-Fu Wu, Jennifer Art, Archie Patel, Nadja Zeltner

“…Summary: Accessing untransformed human primary parasympathetic neurons is extremely challenging due to their inaccessibility in patients. …”
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Threats to validity in the design and conduct of preclinical efficacy studies: a systematic review of guidelines for in vivo animal experiments. by Valerie C Henderson, Jonathan Kimmelman, Dean Fergusson, Jeremy M Grimshaw, Dan G Hackam

“…Please see later in the article for the Editors' Summary.…”
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Protocol for the induction of human spinal motor neurons from human induced pluripotent stem cells for studying amyotrophic lateral sclerosis by Selena Setsu, Satoru Morimoto, Shiho Nakamura, Fumiko Ozawa, Hideyuki Okano

“…Summary: Here, we present a protocol for inducing spinal lower motor neurons (LMNs) from human induced pluripotent stem cells (iPSCs). …”
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Protocol for investigating chromatin architecture in adult skeletal muscle stem cells using high-throughput chromosome conformation capture by Jinjin Kang, Yaxin Zeng, Nianle Li, Liangqiang He, Huating Wang, Kun Sun, Yu Zhao

“…Summary: 3D genome architecture is pivotal for gene transcription and cellular lineage development. …”
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Islamic Entrepreneurship: A Study of the Waqf Well Story Saidina Uthman r.a. by Khaulah Hilaluddin, Mohd Nazri Muhayiddin, Mohd Zulkifli Muhammad, Anis Amira Ab Rahman

“…The study's findings shed light on the Companions of the Prophet's practice of Islamic entrepreneurship, highlighting their character, behavior, thought, action, and strategy to seize opportunities, prioritize the well-being of Muslims, and achieve success in the afterlife. In summary, this story also shows how important it is to prioritize mastery of religious knowledge in understanding creed, worship, and morals while venturing into other fields to produce an execution and thoughts that align with Islamic principles. …”
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Protocol for real-time measurement of mitochondrial bioenergetics in 3D-cultured brain tumor stem cells using the Resipher system by Cloé Tessier, Maxime Toujas, Antonio C. Pagano Zottola, Andreas Bikfalvi, Thomas Mathivet, Thomas Daubon, Lucie Brisson, Audrey Burban, Ahmad Sharanek

“…Summary: Aberrant mitochondrial function can lead to severe human diseases, including neurodegenerative diseases and cancer. …”
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The Surgical Approach to a Medial Epicondylectomy in Cubital Tunnel Syndrome by Abdus S. Burahee, MBChB, Liron S. Duraku, PhD, Rajive Jose, MD, Michiel J. Zuidam, PhD, Dominic M. Power, MB, BChir

“…Summary:. Cubital tunnel syndrome, a common neuropathy of the upper limb, presents with sensory and motor symptoms, impacting hand function and grip strength. …”
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Protocol for dual metabolomics and proteomics using nanoflow liquid chromatography-tandem mass spectrometry by Weiwei Lin, Fatemeh Mousavi, Benjamin C. Blum, Christian F. Heckendorf, Matthew Lawton, Noah Lampl, Ryan Hekman, Mark McComb, Andrew Emili

“…Summary: Nanoflow liquid chromatography-tandem mass spectrometry (nLC-MS) benefits untargeted metabolomics by enhancing sensitivity and integrating proteomics for the same sample. …”
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Calvarial Resurfacing for the Surgical Management of Ossified Cephalohematoma Using Computer-assisted Planning and 3 Dimensional–printed Guides by Taylor Blount, BS, Jordan Whittles, BS, Joseph Lopez, MD, MBA, FAAP, FACS

“…Summary:. Cephalohematoma, a common complication of birth trauma, typically resolves spontaneously within the first month of life. …”
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Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae) by Lourdes E. Córdoba, Alicia R. Pérez de Rosas, Beatriz A. García, María M. Stroppa

“…Summary: RNA interference (RNAi) is a technique that enables post-transcriptional gene silencing in organisms. …”
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Protocol for differentiating human pluripotent stem cells into midbrain organoids for targeted microinjection of viruses by Mengdan Tao, Qi Chen, WeiWei Gao, Shanshan Wu, Yan Liu

“…Summary: Midbrain organoids serve as a valuable model for studying brain development and disease. …”
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Protocol for Agrobacterium-mediated transformation of tomato and flow cytometry-based ploidy confirmation by Yiming Wang, Yixuan Feng, Tai Wang, Lingtong Liu

“…Summary: Here, we present an Agrobacterium-mediated transformation protocol for tomato, Solanum lycopersicum cv. …”
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The Effect of Educational Games on Creativity: A systematic Review by Mohadeseh Ziaee, Mohsen Rostamy-MalKhalifeh, Tofigh Allahviranloo

“…Results: The study's outcomes revealed that scholars have predominantly concentrated their inquiries on the ramifications of educational games concerning critical thinking and problem-solving, the correlation between educational games and motivational aspects of learning, the effects of both digital and physical gaming environments on creativity, the significance of social interactions within educational gaming contexts, the influence of educational games on cognitive and social-cognitive competencies, and frameworks for evaluating creativity within educational games. Conclusions: In summary, these thematic findings suggest that educational games constitute a potent instrument for augmenting creativity among students, contingent upon their thoughtful design and execution.…”
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Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures by Mariana Rebocho da Costa, Ana Ribeiro, Raquel Quitéria, Dalila Neves-Silva, Leonor Saúde

“…Summary: The vascular repair, a pivotal element of the spinal cord (SC) injury response, has been notably neglected in zebrafish research. …”
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Protocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays by Stefano Conti Nibali, Andrea Magrì, Angela Messina, Armin Wagner, Ramona Duman, Vito De Pinto, Cristian Turato, Cristina Arrigoni, Marco Lolicato

“…Summary: Voltage-dependent anion channel 1 (VDAC1) is a key protein in cellular metabolism and apoptosis. …”
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Protocol for recording neuronal activity in food and cocaine self-administration in freely behaving mice using calcium imaging by Jiale Wang, Bin Wang, Michael W. Wang, Nicholas J. Beacher, Da-Ting Lin, Yan Zhang

“…Summary: In vivo calcium imaging has revealed complex neural activity during operant conditioning. …”
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Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel by Connie S. Chamberlain, Daniel M. Tremmel, Ayesha Palwasha Khan, Ellen Abad Santos, Deep Kapadia, Caterra Leavens, Katherine M. Gorski, Rawan Hamadeh, Austin K. Feeney, Jon Odorico, Sara D. Sackett

“…Summary: We present a protocol for transforming human pancreatic tissue into a decellularized and delipidized extracellular matrix (ECM) for hydrogel/scaffold applications. …”
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