Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique by Tomofumi Yamamoto, Fumihiko Urabe, Yusuke Yoshioka, Yusuke Yamamoto, Takahiro Ochiya

“…Summary: Extracellular vesicles (EVs) play a key role in cancer development and cellular homeostasis by transferring the biological cargo to recipient cells. …”
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Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA by Lyan van der Sleen, Marco van den Noort, Laura-Marie Silbermann, Bert Poolman, Katarzyna Tych

“…Summary: OpuA is an osmoregulatory ATP-binding cassette transporter that undergoes different conformations upon varying salt concentrations and ligand conditions. …”
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<IN> IIS QUI AD ME TAMQUAM CHRISTIANI DEFEREBANTUR, HUNC SUM SECUTUS MODUM. ŚRODKI DOWODOWE ZASTOSOWANE W PROCESIE CHRZEŚCIJAN PONTYJSKICH W RELACJI PLINIUSZA MŁODSZEGO (EP. 10,96)... by Maciej Jońca

“…A governor asked those who refused to abandon their faith before executing them. The execution did not apply to Roman citizens who, according to law, were sent to Rome. …”
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Protocol to assess the impact of sperm RNA on mouse preimplantation embryos using microinjection by Grace S. Lee, Natalie A. Trigg, Colin C. Conine

“…Summary: Zygotic microinjections show that sperm RNAs transmit non-genetically inherited phenotypes to offspring and influence embryonic development. …”
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Protocol for the detection of proteolytic activity in female Aedes aegypti mosquito midgut extracts using fluorogenic AMC protease substrates by Abigail G. Ramirez, Daniel Fong, My Anh Le, Jun Isoe, Alberto A. Rascón, Jr.

“…Summary: Female Ae. aegypti mosquitoes utilize midgut proteolytic enzymes to digest blood meal proteins, but activity detection has largely depended on the use of chromogenic substrates. …”
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Protocol for the synthesis and activation of hydrogels with photocaged oligonucleotides by Danielle Herubin, Amanda Yang, Saanvi Gaddam, Katelyn Mathis, Brian Meckes

“…Summary: Hydrogels with spatial-temporal control over chemical and physical properties allow for the creation of cellular niches with controllable properties that better mimic tissue environments. …”
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Protocol for monitoring Yersinia pestis colonization of the proventriculus in the flea Xenopsylla cheopis using microscopy by Amélie Dewitte, Elisabeth Werkmeister, Florent Sebbane, Sébastien Bontemps-Gallo

“…Summary: Colonization of the flea proventriculus is essential for increasing the likelihood of Yersinia pestis transmission. …”
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Protocol to co-culture SCLC cells with human CD8+ T cells to measure tumor cell killing and T cell activation by Subhamoy Chakraborty, Subhasree Sridhar, Kedwin Ventura, Ramja Sritharan, Anna Tocheva, Triparna Sen

“…Summary: Small-cell lung cancer (SCLC) is an aggressive malignancy with immunosuppressive tumor microenvironment limiting immunotherapy efficacy. …”
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A guide for nanomechanical characterization of soft matter via AFM: From mode selection to data reporting by Eunyoung Kim, Alexandra L. Ramos Figueroa, Max Schrock, Elizabeth Zhang, Christina J. Newcomb, Zhenan Bao, Lukas Michalek

“…Summary: Atomic force microscopy (AFM) enables high-resolution mechanical characterization of soft materials at the nanoscale. …”
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Protocol for generation of transmitochondrial cybrids under pyruvate/uridine-supplemented conditions using a microfluidic device by Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda, Yui Harada, Yoshikazu Yonemitsu

“…Summary: Under conventional approaches, selective culture with a pyruvate/uridine (PU)-free medium is essential for generating transmitochondrial cybrids. …”
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Protocol for the measurement of secreted acetylcholine in the lumen of mouse intestine using LC-MS by Evgenia Turtoi, Sylvain Henry, Charlène Joséphine, Marième Ndjim, Imène Gasmi, François Gerbe, Philippe Jay, Andrei Turtoi

“…Summary: Acetylcholine (ACh) is a small-molecule neurotransmitter that regulates a wide range of neuronal and non-neuronal physiological functions. …”
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Protocol for assessing auditory function, cochlear synaptopathy, and afferent terminal morphology in laboratory mice by Indra Pal, Atri Bhattacharyya, Babak V-Ghaffari, Essence Devine Williams, Maolei Xiao, Mark Allen Rutherford, María Eulalia Rubio

“…Summary: Here, we present a protocol for evaluating auditory function and synaptic molecular-structural integrity in mice, specifically female GluA3-knockout (GluA3KO) mice raised in quiet or ambient sound levels. …”
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REGULACJA PRAWNA INTERPELACJI POSELSKICH W SEJMIE ŚLĄSKIM W LATACH 1922-1939 by Józef Ciągwa

“…A specific procedure of the execution of this right was to be determined by the Silesian Act on the Internal Government of the Silesian Voivodship. …”
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Protocol to study the contribution of tendon lineage in the development of mouse temporomandibular joint condylar cartilage using 3D visualization by Paria Dehghanian, Vishal Gokani, Matthew J. Kesterke, Yan Jing, Chi Ma

“…Summary: Tendon cells have been shown to play a critical role during the postnatal growth of the temporomandibular joint (TMJ) condyle by transdifferentiating into chondrocytes. …”
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Protocol for immunofluorescent staining, high-resolution imaging, and spatial projection of Schlemm’s canal in mouse whole-mount corneal limbus tissue by Weifa Wang, Peiyao Peng, Wenfo Hu, Xuyang Liu, Liming Yang, Rong Ju, Feng Zhang

“…Summary: Schlemm’s canal (SC) is an endothelium-lined conduit with uniquely combined features of blood vessels and lymphatic vessels required for aqueous humor drainage from the eye. …”
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Protocol for measuring D-amino acid transport in mammalian cells using a fluorescent biosensor by Danisha Johal, Keeley G. Hewton, Sadie J. Gale, Amritpal S. Johal, Seth J. Parker

“…Summary: The movement of amino acids into cells and organelles is facilitated by transporter proteins, which support homeostasis, metabolism, and disease progression. …”
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Protocol to detect m22G modification on single tRNA iso-decoders by immuno-northern blotting by Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt

“…Summary: tRNA modifications are highly dynamic and play crucial roles in regulating gene expression. …”
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Protocol for assembling photostable fluorescence-labeled tiny DNA origami blocks to observe kinesin cooperation by Tomoki Kita, Ryota Sugie, Yuki Suzuki, Shinsuke Niwa

“…Summary: Fluorescence-labeled tiny DNA origami blocks (FTOBs) enable observation of kinesin motility and their cooperation with minimal blinking and photobleaching. …”
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Protocol for performing and analyzing a live-cell imaging-based microglia phagocytosis assay using AI on the Olympus ScanR system by Alexander Zähringer, Janaki Manoja Vinnakota, Tobias Wertheimer, Marie Follo, Robert Zeiser

“…Summary: Various neurological disorders show dysregulation of microglial function, including phagocytosis, necessitating accurate and efficient analysis. …”
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Protocol to analyze tRNA and rRNA processing using biotin-labeled probes by Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt

“…Summary: Mature tRNAs and rRNAs are derived from larger precursor transcripts through intricate endo- and exonuclease processing steps. …”
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