A non-hypothesis-driven practical laboratory activity on functional metagenomics: “fishing” protein-coding DNA sequences from microbiomes by Melissa Morra, Denise Marradi, Luca Gandini, Vittorio Ivagnes, Giulia Ottolini, Alessandro Bovio, Grace Jabali, Lorenzo Maraschi, Ifeoluwa Ayomide Dada, Tonderai Vitalis Chawanda, Martina Gorla, Olga Tarasiuk, Chiara Mocchetti, Maria Felicia Soluri, Maria Felicia Soluri, Francesca Boccafoschi, Daniele Sblattero, Diego Cotella

“…Here, we describe a simple protocol for the construction of a metagenomic DNA library, optimized and tested by a team of undergraduate biotechnology students. …”
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The biological effect of a combination of platelet lysate and amniotic membrane in buccal epithelium culture by E. V. Chentsova, N. V. Borovkova, P. V. Makarov, D. A. Bozhenko, I. N. Ponomarev, M. V. Storozheva, M. S. Makarov

“…Purpose: To study the biological effect of a combination of platelet lysate and amniotic membrane, preserved by various techniques, on human buccal epithelium culture. Materials and methods. Human amnion transplants were preserved using 3 methods: silicate drying, lyophilization, cryopreservation. …”
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Breeding value of spring durum wheat accessions for performance traits under environment of Ukrainian Forest-Steppe by С. О. Хоменко, В. С. Кочмарський, І. В. Федоренко, М. В. Федоренко

“…To identify spring durum wheat accessions with high yield level and by yield components for their involvement in breeding programs as a source material. Methods. Field, statistical. Results. …”
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Life Cycle Assessment of Municipal Solid Waste Management Strategies in Babolsar: Evaluating Environmental Impacts and Energy Consumption by Babak Sheydaei, Gholamreza Nabi, Fatemeh Yazdian

“…The implications of these findings extend to similar urban areas seeking to optimize their waste management practices for better environmental outcomes.…”
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Current status and obstacles in the sustainable synthesis of biohydrogen from microalgal species by Akshay Loyte, Jiwak Suryawanshi, Sacheth Sri Kiran Bellala, Roshan V. Marode, Yuvarajan Devarajan

“…Techniques such as optimizing growth conditions, genetic engineering, using microalgal consortia, innovative photobioreactor design, and enzymatic processes are used to develop a concise understanding and thus formulate methods and techniques to increase hydrogen production from microalgae. …”
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Antioxidant Activities of Glycyrrhiza glabra L. and Momordica charantia L. Collected From Kahramanmaraş Charantia L. Collected From Kahramanmaraş Kahramanmaraş, Türkiye by Ayşegül Gölcü, Hatice Elmacioglu, Beril S. Kaya, Abdullah Al Faysal

“…The antioxidant capacity of the crude products from two Kahramanmaraş plants, licorice (Glycyrrhiza glabra) and bitter melon (Momordica charantia L.), was examined. Methods: This study systematically investigates, for the first time, various combinations of temperature, stirring rpm and time, and solvent to optimally extract the bioactive properties from these plants. …”
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Fine‐Tuning Porous Structure of Zirconium‐Based Metal–Organic Frameworks for Efficient Separation and Purification of Astaxanthin by Defect Engineering by Xin Na, Shanghua Xing, Mingqian Tan, Wentao Su

“…Abstract Efficient separation of bioactive compounds from nature source, particularly that of astaxanthin (AXT), remains challenging due to their low content in complicated matrix and readily degradable structure. …”
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Integrating wastewater surveillance for future pandemic preparedness by tracking SARS-CoV-2 RNA in municipal wastewater in Southwest Iran by Rozhan Feizi, Neematollah Jaafarzadeh, Masoud Panahi Fard, Niloofar Neisi, Ali Zafarzadeh, Mohammad Sabaghan, Ali Veisi

“…Further research is required to investigate seasonal variations in virus shedding, optimize detection methods to improve sensitivity, and further investigate the relationship between wastewater viral loads and disease transmission dynamics to enhance pandemic preparedness and response.…”
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A systematic review and meta-analysis of the impact of stretching techniques on balance performance by Weishuai Guo, Youngsuk Kim, Chaojie Wu, Sukwon Kim

“…Meta-regression identified stretching duration as a significant source of heterogeneity, with durations between 20 and 200 s associated with better balance outcomes. …”
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Research and Construction of Knowledge Map of Golden Pomfret Based on LA-CANER Model by Xiaohong Peng, Hongbin Jiang, Jing Chen, Mingxin Liu, Xiao Chen

“…To address the issues of fragmented species information, low knowledge extraction efficiency, and insufficient utilization in the aquaculture domain, the main objective of this study is to construct the first knowledge graph for the Golden Pomfret aquaculture field and optimize the named entity recognition (NER) methods used in the construction process. …”
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Use of whole body PET scan in patients with infective endocarditis may impact care of those with intravascular devices: results from a comparative retrospective cohort study by Jason Park, Kaitlyn Simpson, Megan Neils, James Riddell

“…Changes in management included further workup, performance of a source control procedure, or a change in the antibiotic regimen. …”
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Multi-Domain Feature Incorporation of Lightweight Convolutional Neural Networks and Handcrafted Features for Lung and Colon Cancer Diagnosis by Omneya Attallah

“…It also applies feature selection based on minimum redundancy maximum relevance to the integrated deep and handcrafted features, guaranteeing optimal computational efficiency and high diagnostic accuracy. …”
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Residual capsule network with threshold convolution and attention mechanism for forest fire detection using UAV imagery by Soufiane Ben Othman, Obaid Ali

“…Evaluated on a comprehensive UAV-sourced dataset of 14,140 images, ResCaps-TC-Attn-Fire achieves an impressive 99.78% accuracy, 99.7% precision, and 99.8% recall, surpassing existing methods like YOLOv3 (85.2%), ABi-LSTM (96.2%), and Enhanced YOLOv8n (99.0%). …”
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Machine learning and spatio-temporal analysis of meteorological factors on waterborne diseases in Bangladesh. by Arman Hossain Chowdhury, Md Siddikur Rahman

“…Therefore, this study aimed to fill this gap by investigating the spatio-temporal pattern and identifying the best tree-based ML models for determining the meteorological factors associated with waterborne diseases in Bangladesh.<h4>Methods</h4>This study used district-level reported cases of waterborne diseases (cholera, amoebiasis, typhoid and hepatitis A) obtained from the Bangladesh Bureau of Statistics (BBS) and meteorological data (temperature, relative humidity, wind speed, and precipitation) sourced from NASA for the period spanning 2017 to 2020. …”
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Impact of artificial intelligence-driven urban renewal strategies green economic efficiency and resident health in China by Jing Peng, Xin Fu, Yufan Peng, Yang Ding

“…It further explores how industrial structure rationalization, upgrading, and technological innovation capacity moderate these effects, aiming to provide actionable insights for sustainable urban governance.MethodsTo account for potential policy spillover effects, the study adopts a Difference-in-Differences (DID) approach integrated with a Spatial Durbin Model (SDM). …”
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The Therapeutic Potential of Cannabidiol in the Management of Temporomandibular Disorders and Orofacial Pain by Karolina Walczyńska-Dragon, Anna Kurek-Górecka, Jakub Fiegler-Rudol, Aleksandra Nitecka-Buchta, Stefan Baron

“…Relevant studies were sourced from PubMed, Scopus, and Web of Science databases. …”
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BoostPolyGlot: A Structured IR Generation-Based Fuzz Testing Framework for GCC Compiler Frontend by Hui Liu, Hanbin Guo, Peng Liu, Tongding Hou

“…The compiler serves as a bridge connecting hardware architecture and application software, converting source code into executable files and optimizing code. …”
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Evaluation of grain yield and quality in maize hybrids of various origin under the conditions of Primorsky Territory by E. S. Butovets, I. N. Danilenko, N. A. Kraskovskaya

“…This study was dedicated to the evaluation and selection of maize hybrids of different origin for important agronomic traits and for protein and oil accumulation in grain under the conditions of Primorsky Territory, as well as for their possible use as source material for breeding.Materials and methods. …”
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Design of e-commerce product price prediction model based on generative adversarial network with adaptive weight adjustment by Abuduaini Abudureheman, Yan Zhao, Aishanjiang Nilupaer

“…Moreover, the AWA-CWGAN algorithm surpasses baseline methods in accuracy (88.8%), precision (88.81%), recall (89.255%), and F1 score (87.95%). …”
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Association between out-of-pocket health expenditure and the disease burden of diabetes mellitus: insights from GBD 2021 by Luyou Dong, Zhifu Dong

“…This study analyzes the relationship between out-of-pocket (OOP) expenditure levels and the disease burden of diabetes mellitus, provides evidence-based recommendations for optimizing OOP expenditure strategies, and seeks to uncover any potential impact of healthcare inequalities on the disease burden of diabetes mellitus.MethodsThis cross-sectional study was performed among 36 countries with varying percentages of OOP payments from Health System in Transition. …”
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